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Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean(Indian sector)water

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Acta Oceanologica Sinica 2015年第8期34卷 73-82页

作者： GUPTA Pratibha BALAJI Raju parani m CHANDRA T S SHUKLA P KUmAR Anil BANDOPADHYAY Rajib Department of Bio-Engineering Birla Institute of Technology Department of Genetic Engineering Kattankulathur Campus SRM University microbiology and Genetics Laboratory Department of Biotechnology Indian Institute of Technology Madras Department of microbiology Maharshi Dayanand University OSSG Department National Centre for Antarctic and Ocean Research Ministry of Earth Sciences Government of India UGC Centre of Advanced Study Department of Botany the University of Burdwan

Fifty-seven bacteria were isolated from Southern Ocean （Indian sector） water samples which were collected from different latitude and longitude of the ocean. All the isolates were able to grow at 4℃, 20℃, 37℃ and tolerable NaCI concentration up to 13.5% （w/v）. 29 out of 57 isolates were identified using 16S rDNA amplification and the sequences were submitted to National Center for Biotechnology Information （NCBI）. All the isolates were classified by using Ribosomal Database Project （RDP） and found that isolates belongs to Proteobacteria and Bacteriodes. The average G＋C content was 56.4%. The isolates were screened for the presence of extracellular enzymes, viz. amylase, catalase, urease, esterase, lipase and protease. The disc diffusion method is used to screen antibiotic production by the isolates against four pathogenic bacteria, viz. Salmonella typhimurium （NCIm 2501）, Staphylococcus aureus （NCIm 2122）, Bacillus subtilis （NCIm 2193）, and Pseudomonas aeruginosa （NCIm 2036）. Nine out of 29 were found to be antibiotic producer.

关键词： Southern Ocean marine bacteria 16S rDNA phylogenetic tree antibacterial

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