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机荷高速公路立体复合改扩建工程水环境治理研究

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交通节能与环保 2024年第3期20卷 131-134页

作者：马盼盼张梦利尹静于慧卿中交第一公路勘察设计研究院有限公司陕西西安710065 西安中交环境工程有限公司陕西西安710065

为探究城市高度建成区高速公路水环境治理问题,本文以深圳市机荷高速公路立体复合改扩建工程为例,论述了城市高度建成区高速公路水环境治理的困境,结合机荷高速公路不同区段径流及污染特征,确定基于海绵设施的水环境治理思路,规划设计... 详细信息

为探究城市高度建成区高速公路水环境治理问题,本文以深圳市机荷高速公路立体复合改扩建工程为例,论述了城市高度建成区高速公路水环境治理的困境,结合机荷高速公路不同区段径流及污染特征,确定基于海绵设施的水环境治理思路,规划设计了基于海绵城市理念的城市高度建成区水环境治理方案,为城市高度建成区高速公路水环境治理提供借鉴。

关键词：高速公路海绵城市水环境治理机荷高速公路

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HFO类典型工质的基础特性及其在冷水机组的循环性能初步分析!

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制冷与空调 2017年第6期17卷 28-32页

作者：贾磊赵盼盼钟瑜何亚峰张秀平王汝金合肥通用机械研究院压缩机技术国家重点实验室

介绍R1234yf,R1234ze(E),R452B,R513A和R515A这5种典型HFO类工质的基础特性,分别针对水冷式和风冷式冷水机组的典型设计工况,开展上述5种工质与R134a,R410A和R22这3种传统工质的循环性能初步对比分析,结果表明:R1234ze(E)和R515A具有较... 详细信息

介绍R1234yf,R1234ze(E),R452B,R513A和R515A这5种典型HFO类工质的基础特性,分别针对水冷式和风冷式冷水机组的典型设计工况,开展上述5种工质与R134a,R410A和R22这3种传统工质的循环性能初步对比分析,结果表明:R1234ze(E)和R515A具有较高的COP和较低的排气温度,但单位容积制冷量偏低;R452B虽然具有较高的单位容积制冷量,但还须在系统方面进行深入优化以提升机组的整体能效。

关键词：制冷剂 HFO 冷水机组制冷循环性能

高通量测序技术分析酱香型白酒下沙轮次堆积过程的微生物多样性

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酿酒 2020年第4期47卷 51-55,59页

作者：姜明慧许玲于文娟董乔娟于盼盼杨庆振白秀彬赵纪文山东扳倒井股份有限公司山东高青256300

研究通过高通量测序技术对酱香型白酒下沙轮次堆积发酵过程中细菌、真菌进行了全面的测序分析,从而解析堆积不同时间节点的微生物的群落变化规律。结果表明,堆积过程中八个时间节点分别检测到113个、134个、176个、169个、198个、207个... 详细信息

研究通过高通量测序技术对酱香型白酒下沙轮次堆积发酵过程中细菌、真菌进行了全面的测序分析,从而解析堆积不同时间节点的微生物的群落变化规律。结果表明,堆积过程中八个时间节点分别检测到113个、134个、176个、169个、198个、207个、158个、142个细菌属,51个、79个、60个、105个、121个、166个、114个、103个真菌属。堆积过程中细菌优势菌属为乳酸菌,占比可达85%以上,真菌优势菌属均为生香酵母。该结果为进一步研究酱香型白酒的酿造机理提供了前期基础数据。

关键词：高通量测序酱香型白酒堆积酒醅微生物群落结构

高通量测序技术解析扳倒井浓香型白酒窖泥原核微生物群落结构

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酿酒 2021年第3期48卷 81-85页

作者：于文娟许玲杨庆振于盼盼姜明慧董乔娟白秀彬赵纪文山东扳倒井股份有限公司山东高青256300

通过Illumina Miseq 2×300bp高通量测序技术对山东扳倒井股份有限公司浓香型白酒窖泥原核微生物群落结构进行了研究。发现:窖泥中细菌主要为梭菌纲(Clostridia)、拟杆菌纲(Bacteroidia)、甲烷杆菌纲(Methanobacteria)和芽孢杆菌纲(Baci... 详细信息

通过Illumina Miseq 2×300bp高通量测序技术对山东扳倒井股份有限公司浓香型白酒窖泥原核微生物群落结构进行了研究。发现:窖泥中细菌主要为梭菌纲(Clostridia)、拟杆菌纲(Bacteroidia)、甲烷杆菌纲(Methanobacteria)和芽孢杆菌纲(Bacilli);古菌主要为甲烷微菌纲(Methanomicrobia)、甲烷杆菌纲(Methanobacteria)和热原体纲(Thermoplasmata)。同时得出窖池位置对窖泥原核微生物群落结构的丰富度和多样性影响最大,压池时间越长窖泥菌落结构的丰富度和多样性越高,多粮发酵窖泥菌落结构与单粮发酵窖泥菌落结构既有特异性也保持相关性。

关键词：浓香型白酒窖泥高通量测序原核微生物群落结构

Integrating thermal energy storage and microwave absorption in phase change material-encapsulated core-sheath MoS_(2)@CNTs

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Journal of Energy Chemistry 2023年第9期84卷 41-49页

作者： panpan Liu Yang Li Zhaodi Tang Junjun Lv Piao Cheng Xuemei Diao yu Jiang Xiao Chen Ge Wang Institute of Advanced Materials Beijing Normal UniversityBeijing 100875China College of Nuclear Science and Technology Beijing Normal UniversityBeijing 100875China Beijing Advanced Innovation Center for Materials Genome Engineering Beijing Key Laboratory of Function Materials for Molecule&Structure ConstructionSchool of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083China

Developing advanced nanocomposite integrating solar-driven thermal energy storage and thermal management functional microwave absorption can facilitate the cutting-edge application of phase change materials(PCMs).To conquer this goal,herein,two-dimensional MoS_(2) nanosheets are grown in situ on the surface of one-dimensional CNTs to prepare core-sheath MoS_(2)@CNTs for the encapsulation of paraffin wax(PW).Benefiting from the synergistic enhancement photothermal effect of MoS_(2) and CNTs,MoS_(2)@CNTs is capable of efficiently trapping photons and quickly transporting phonons,thus yielding a high solar-thermal energy conversion and storage efficiency of 94.97%.Meanwhile,PW/MoS_(2)@CNTs composite PCMs exhibit a high phase change enthalpy of 101.60 J/g and excellent lo ng-term thermal storage durability after undergoing multiple heating-cooling *** attractively,PW/MoS_(2)@CNTs composite PCMs realize thermal management functional microwave absorption in heat-related electronic application scenarios,which is superior to the single microwave absorption of traditional *** minimum reflection loss(RL) for PW/MoS_(2)@CNTs is-28 dB at 12.91 GHz with a 2.0 mm *** functional integration design provides some insightful references on developing advanced microwave absorbing composite PCMs,holding great potential towards high-efficiency solar energy utilization and thermally managed microwave absorption fields.

关键词： Phase change materials Core-sheath MoS_(2)@CNTs Solar-thermal energy conversion Thermal energy storage Microwave absorption

浓香型白酒酒醅充氧时间对蒸馏酒质的影响研究

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酿酒 2020年第5期47卷 88-89页

作者：许玲白秀彬于盼盼董乔娟于文娟姜明慧杨庆振张聪聪山东扳倒井股份有限公司山东高青256300

研究针对度夏后的浓香型窖池底部酒醅进行堆积放置,自然接触空气,氧化糟醅,设定不同时间,然后进行蒸馏,发现放置24h后的酒醅蒸馏后酒质明显提高,且清蒸后的糟醅活力较好,可以作为下轮次面糟使用。

关键词：浓香型白酒酒醅充氧酒质

酵母双杂交文库筛选及N型钙离子通道与BART相互作用的初步分析

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医学分子生物学杂志 2006年第1期3卷 22-25,F0003页

作者：朱慧琴于盼盼林琳杭勤陆佩华黄立东上海第二医科大学神经生物学实验室上海市200025

目的应用酵母双杂交技术筛选N型钙离子通道相关蛋白,并对候选蛋白BART(binder of arltwo)进行初步分析。方法用N型钙离子通道α1亚基C端630个氨基酸残基肽段(Calcium channel630a-mino acids,CaCh630)作为探针,运用酵母双杂交技术筛选... 详细信息

目的应用酵母双杂交技术筛选N型钙离子通道相关蛋白,并对候选蛋白BART(binder of arltwo)进行初步分析。方法用N型钙离子通道α1亚基C端630个氨基酸残基肽段(Calcium channel630a-mino acids,CaCh630)作为探针,运用酵母双杂交技术筛选出与之相互作用的基因。通过PCR扩增并克隆候选基因BART,然后分别构建带有红色荧光蛋白标签的表达载体pDsRed-BART和带绿色荧光蛋白标签的pEGFP-CaCh630,共转染海马神经元后,用激光共聚焦扫描显微镜观察这两个外源蛋白在细胞中的定位情况。结果应用酵母双杂交技术筛选出6个相关基因,体外共转染试验发现BART和CaCh630分子在海马神经元内有共定位现象,进一步证明其可能存在相互作用。结论BART可能在维持钙离子通道细胞骨架中定位起重要作用。

关键词：酵母双杂交技术钙离子通道共定位

RcSPL1-RcTAF15b regulates the flowering time of rose (Rosa chinensis)

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Horticulture Research 2023年第6期10卷 182-194页

作者： Rui yu Zhiying Xiong Xinhui Zhu panpan Feng Ziyi Hu Rongxiang Fang yuman Zhang Qinglin Liu Department of Ornamental Horticulture College of HorticultureChina Agricultural UniversityBeijing 100193China National Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciencesand National Plant Gene Research CenterBeijing 100101China University of Chinese Academy of Sciences Beijing 101408China

Rose(Rosa chinensis),which is an economically valuable floral species worldwide,has three types,namely once-flowering(OF),occasional or re-blooming(OR),and recurrent or continuous flowering(CF).However,the mechanism underlying the effect of the age pathway on the duration of the CF or OF juvenile phase is largely *** this study,we observed that the RcSPL1 transcript levels were substantially upregulated during the floral development period in CF and OF ***,accumulation of RcSPL1 protein was controlled by *** ectopic expression of RcSPL1 in Arabidopsis thaliana accelerated the vegetative phase transition and ***,the transient overexpression of RcSPL1 in rose plants accelerated flowering,whereas silencing of RcSPL1 had the opposite ***,the transcription levels of floral meristem identity genes(APETALA1,FRUITFULL,and LEAFY)were significantly affected by the changes in RcSPL1 ***15b protein,which is an autonomous pathway protein,was revealed to interact with *** silencing and overexpression of RcTAF15b in rose plants led to delayed and accelerated flowering,***,the study findings imply that RcSPL1–RcTAF15b modulates the flowering time of rose plants.

关键词： TAF flowering floral

Increased leptin by hypoxic-preconditioning promotes autophagy of mesenchymal stem cells and protects them from apoptosis

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Science China(Life Sciences) 2014年第2期57卷 171-180页

作者： WANG LiHan HU XinYang ZHU Wei JIANG Zhi ZHOU yu CHEN panpan WANG JianAn Cardiovascular Key Lab of Zhejiang Province the Second Affiliated Hospital School of Medicine Zhejiang University

Autophagy is the basic catabolic progress involved in cell degradation of unnecessary or dysfunctional cellular *** has been proven that autophagy could be utilized for cell survival under ***-preconditioning(HPC)could reduce apoptosis induced by ischemia and hypoxia/serum deprivation(H/SD)in bone marrow-derived mesenchymal stem cells(BMSCs).Previous studies have shown that both leptin signaling and autophagy activation were involved in the protection against apoptosis induced by various stress,including ***,it has never been fully understood how leptin was involved in the protective effects conferred by *** the present study,we demonstrated that HPC can induce autophagy in BMSCs by increased LC3-II/LC3-I ratio and autophagosome ***,similar effects were also observed when BMSCs were pretreated with *** beneficial effects offered by HPC were absent when BMSCs were incubated with autophagy inhibitor,3-methyladenine(3-MA).In addition,down-regulated leptin expression by leptin-shRNA also attenuated HPC-induced autophagy in BMSCs,which in turn was associated with increased apoptosis after exposed to sustained H/***,increased AMP-activated protein kinase phosphorylation and decreased mammalian target of rapamycin phosphorylation that were observed in HPC-treated BMSCs can also be attenuated by down-regulation of leptin *** data suggests that leptin has impact on HPC-induced autophagy in BMSCs which confers protection against apoptosis under H/SD,possibly through modulating both AMPK and mTOR pathway.

关键词： BMSCs autophagy hypoxic-preconditioning leptin apoptosis