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Science China(Life Sciences) 2005年第1期48卷 89-96页

作者： WANG Hanqian ZHANG Xueyong WANG Hongmei pang binshuang Key Laboratory of Crop Germplasm and Biotechnology Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural SciencesBeijing100081China

Wheat high molecular weight glutenin subunits(HMW-GS)1Bx14 and 1By15 isolated by preparative SDS-PAGE are used as antigen to immunize BALB/c *** inoculation of the antigen is *** intra-peritoneal injection is completed 3 days before fusion with myeloma cell(SP2/0)via *** fusion cells are selected by indirect enzyme-linked immuno-sorbent assay(ELISA).Positive hybrid cells are further verified three times by limit dilution of the culture cells.A hybridoma cell line is successfully *** monoclonal antibody belongs to IgG1 *** immunoblotting,the antibody binds to all HMW-GS of *** cultivars,but does not bind to other storage proteins in seeds of *** result is consisting with the high homology in amino acid sequences among the HMW glutenin subunits in *** antibody also binds to HMW-GS storage proteins in Aegilops squarrosa and ***(durum wheat).Furthermore,it also binds to HMW storage proteins in Secale cereale(rye),Hordeum vulgare(barley).However,it never binds seed storage proteins in other cereals such as maize,oat,rice,foxtail millet,sorghum *** antigen determinant recognized by the antibody has been located within hexapeptide[PGQGQQ]or/and nonapeptide[GYYPTSPQQ]in the central repetitive region of HMW-GS.

关键词： wheat HWM-GS monoclonal antibody antigenic determinant immunoblotting.

Consensus linkage map construction and QTL mapping for eight yield-related traits in wheat using the BAAFS Wheat 90K SNP array

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Journal of Integrative Agriculture 2024年第11期23卷 3641-3656页

作者： Lihua Liu Pingping Qu Yue Zhou Hongbo Li Yangna Liu Mingming Zhang Liping Zhang Changping Zhao Shengquan Zhang binshuang pang Institute of Hybrid Wheat Bejing Academy of Agriculture and Forestry SciencesBeijing 100097China The Municipal Key Laboratory of Molecular Genetic of Hybrid Wheat Bejing Academy of Agriculture and Forestry SciencesBeijing 100097China Key Laboratory of Crop DNA Fingerprinting Innovation and Utilization(Co-construction by Ministry and Province) Ministry of Agriculture and Rural AffairsBeijing 100097China

Identifying stable quantitative trait loci(QTLs)for yield-related traits across populations and environments is crucial for wheat breeding and genetic *** maps also play important roles in wheat genetic and genomic *** the present study,a wheat consensus map was constructed using a doubled haploid(DH)population derived from Jinghua 1×Xiaobaidongmai(JX),an F_(2)population derived from L43×Shanxibaimai(LS)and the BAAFS Wheat 90K SNP array single nucleotide polymorphism(SNP)array.A total of 44,503 SNP markers were mapped on the constructed consensus map,and they covered 5,437.92 cM across 21 *** consensus map showed high collinearity with the individual maps and the wheat reference genome IWGSC RefSeq *** data on eight yield-related traits were collected in the JX population,as well as the F_(2:3)and F_(2:4)populations of LS,in six,two and two environments,respectively,and those data were used for QTL *** composite interval mapping(ICIM)identified 32 environmentally stable QTLs for the eight yield-related *** them,four QTLs(***-4B,***-4B,***-4B,and ***-5A.3)were detected across mapping populations and environments,and nine stable QTLs(***-1D,***-2B,***-3D,***-3D,***-4B,***-5D,***-6A.1,***-6A,and ***-6D)are likely to be *** physical region of 17.25–44.91 Mb on chromosome 4B was associated with six yield-related traits,so it is an important region for wheat *** physical region around the dwarfing gene Rht24 contained QTLs for kernel length(KL),kernel width(KW),spike length(SL),and thousand-grain weight(TGW),which are either from a pleiotropic effect of Rht24 or closely linked *** the stable QTLs,254 promising candidate genes were *** them,TraesCS5A03G1264300,TraesCS1B03G0624000 and TraesCS6A03G0697000 are particularly noteworthy since their homologous genes have similar functions for the corresp

关键词： consensus map QTL yield-related traits wheat(Triticum aestivum L.)

高粱DNA导入引起小麦HMW-GS的变异及其品质改良和变异机理分析

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中国粮油学报 2011年第1期26卷 15-19页

作者：欧巧明倪建福崔文娟庞斌双甘肃省农业科学院生物技术研究所兰州730070 北京市农林科学院杂交小麦工程技术研究中心北京100089

采用花粉管通道法,将高粱(Sorghum bicolor L.)基因组DNA导入粉质籽粒的稳定品系89122中,后代经多代连续选择优良变异系,并进行了高分子质量麦谷蛋白亚基的SDS-PAGE电泳检测和品质化验分析。获得1个稳定遗传变异新品种,与导入受体89122... 详细信息

采用花粉管通道法,将高粱(Sorghum bicolor L.)基因组DNA导入粉质籽粒的稳定品系89122中,后代经多代连续选择优良变异系,并进行了高分子质量麦谷蛋白亚基的SDS-PAGE电泳检测和品质化验分析。获得1个稳定遗传变异新品种,与导入受体89122相比,后代新品系2001502-23含有7+8、5+10优质亚基,HMW-GS发生明显变异,Gul D1位点基因发生等位变异,其表达产物由原来(89122)的2+12亚基为5+10亚基;其品质指标较受体得到改善,从而实现了受体小麦HMW-GS基因的遗传转化和品质改良。说明利用花粉管通道法完全可以实现小麦HMW-GS基因的转化和品质目标性状的遗传改良。而生物诱变是其可能的遗传转化和变异机理之一。

关键词：小麦高粱DNA 花粉管通道法高分子质量谷蛋白亚基(HMW-GS) 等位变异

Improvement of Cold Recycled Mixture Performance Based on Improved Density Test Method and RAP Characteristics

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 79-87页

作者： HAN Zhanchuang pang Yafeng LIN Hongwei China First Highway Engineering Group Co. LtdBeijing 100024China The Key Laboratory of Road and Traffic Engineering Ministry of EducationTongji UniversityShanghai 201804China Guangzhou Baiyun International Airport Co. Ltd.Guangzhou 510470China

The available test methods for optimal moisture content of cold recycled mixture(CRM)as well as its bulk specific gravity,and theoretical maximum relative density were analyzed in this *** test improvements were suggested to improve test control of the CRM road performance based on the discovered ***,the properties of reclaimed asphalt pavement(RAP),including the content of old asphalt,penetration index,passing rate of 4.75 mm sieve,and gradation change rate after extraction,were *** effects of RAP characteristics on splitting tensile strength,water stability,the high-and low-temperature performance of emulsified asphalt CRM were *** results show that the optimum moisture content of CRM should be determined when the compaction work matches the specimen’s molding *** the analyzed methods of bulk specific gravity assessment,the dry-surface and CoreLok methods provide more robust and accurate results than the wax-sealing method,while the dry-surface method is the most *** modified theoretical maximum relative density test method is proposed,which can reduce the systematic error of the vacuum test *** following RAP-CRM trends can be *** lower the content of old asphalt and the smaller the change rate of gradation,the smaller the voids and the better the water stability of *** greater the penetration of old asphalt,the higher the fracture work and low-temperature splitting *** greater the penetration,the higher the passing rate of 4.75 mm sieve after extraction,and the worse the high-temperature performance of CRM.

关键词： RAP volume parameters high-temperature performance low-temperature performance water stability

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表土干旱和根信号对春小麦产量形成的影响

应用生态学报 1999年第6期10卷 689-695页

作者：郭安红李凤民李召祥庞斌双山仑兰州大学干旱农业生态国家重点实验室兰州730000 甘肃省农业科学院

本实验模拟大田条件，探讨一定程度水分亏缺下春小麦能否产生非水力根信号，以及对春小麦产量形成的影响．用３个春小麦品种，３个水分处理：充分供水（ＣＴ），上层供水（ＤＩｕ）和上层干旱下层供水（ＤＩｄ）．出苗后１７～２... 详细信息

本实验模拟大田条件，探讨一定程度水分亏缺下春小麦能否产生非水力根信号，以及对春小麦产量形成的影响．用３个春小麦品种，３个水分处理：充分供水（ＣＴ），上层供水（ＤＩｕ）和上层干旱下层供水（ＤＩｄ）．出苗后１７～２８ｄ，非充分供水处理出现了植物叶片水势未显著改变而气孔导度和蒸腾作用却显著下降的现象，这是非水力根信号的典型特征．随着时间的推移，植物叶片水势同对照处理相比显著下降．在本试验的３个品种中，Ｂ品种上层根系的比根重（ＳＲＷ，单位根长的根重）在出苗后３４ｄ和出苗后５４ｄ较Ａ、Ｃ品种高，且在出苗后５４ｄ上层根重的绝对量也显著高于Ａ、Ｃ品种的对应处理，对Ｂ品种获得较高的产量十分有利．Ｃ品种同Ａ、Ｂ品种相比，在出苗后３４ｄ和５４ｄ，ＤＩｕ处理中总根重、总根长分别向上层根重和上层根长分配的比例较Ａ、Ｂ品种高，中层根重和中层根长的相对较低；而ＤＩｄ处理中表现的趋势正好相反．以上表明Ｃ品种对浅层土壤水分十分敏感，其根系的可塑性较强，也反映了其较高的根信号敏感性．相对而言，Ａ品种和Ｂ品种根系的可塑性较弱，但这两个品种的籽粒产量显著高于Ｃ品种．

关键词：干旱春小麦根信号气孔导度产量表土

Cholesterol metabolism: physiological versus pathological aspects in intracerebral hemorrhage

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Neural Regeneration Research 2025年第4期20卷 1015-1030页

作者： Ruoyu Huang Qiuyu pang Lexin Zheng Jiaxi Lin Hanxi Li Lingbo Wan Tao Wang Department of Forensic Science School of Basic Medicine and Biological SciencesSuzhou Medicine College of Soochow UniversitySuzhouJiangsu ProvinceChina

Cholesterol is an important component of plasma membranes and participates in many basic life functions,such as the maintenance of cell membrane stability,the synthesis of steroid hormones,and *** plays a key role in the establishment and maintenance of the central nervous *** brain contains 20%of the whole body’s cholesterol,80%of which is located within myelin.A huge number of processes(e.g.,the sterol regulatory element-binding protein pathway and liver X receptor pathway)participate in the regulation of cholesterol metabolism in the brain via mechanisms that include cholesterol biosynthesis,intracellular transport,and *** brain injuries or diseases involving crosstalk among the processes above can affect normal cholesterol metabolism to induce detrimental ***,we hypothesized that cholesterol-related molecules and pathways can serve as therapeutic targets for central nervous system *** hemorrhage is the most severe hemorrhagic stroke subtype,with high mortality and *** cholesterol levels are associated with the risk of intracerebral ***,secondary pathological changes after intracerebral hemorrhage are associated with cholesterol metabolism dysregulation,such as neuroinflammation,demyelination,and multiple types of programmed cell *** cholesterol accumulation in the brain has been found after intracerebral *** this paper,we review normal cholesterol metabolism in the central nervous system,the mechanisms known to participate in the disturbance of cholesterol metabolism after intracerebral hemorrhage,and the links between cholesterol metabolism and cell *** also review several possible and constructive therapeutic targets identified based on cholesterol metabolism to provide cholesterol-based perspectives and a reference for those interested in the treatment of intracerebral hemorrhage.

关键词： cell death cholesterol metabolism intracerebral hemorrhage myelination therapeutic target

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基于SNP标记的小麦高通量身份鉴定模式

麦类作物学报 2018年第5期38卷 529-534页

作者：刘丽华庞斌双刘阳娜李宏博王娜王拯赵昌平北京市农林科学院杂交小麦工程技术研究中心杂交小麦分子遗传北京市重点实验室北京100097

为探索小麦品种高通量SNP身份鉴定模式,利用wheat 90K SNP芯片对380份小麦品种进行了全基因组扫描、分析和评价,从中筛选出高质量、高分辨率、单拷贝和均匀分布的候选SNP标记384个,能将除近等基因系以外的所有品种区分开;基于组合最优... 详细信息

为探索小麦品种高通量SNP身份鉴定模式,利用wheat 90K SNP芯片对380份小麦品种进行了全基因组扫描、分析和评价,从中筛选出高质量、高分辨率、单拷贝和均匀分布的候选SNP标记384个,能将除近等基因系以外的所有品种区分开;基于组合最优化算法,获得小麦品种高通量鉴定最少SNP位点组合一套,包含14个SNP标记,区分能力与384个SNP标记相同。将14个SNP位点转化成KASP标记,分析选取的95份样品,结果显示,芯片平台和KASP平台上的基因分型结果一致。考虑品种实际鉴定过程中存在样本量大、高度近似品种少等情况,权衡准确、经济、灵活、快速、通量高等检测需求,建议品种高通量身份鉴定可采取"核心位点+扩展位点"的模式进行。本研究为小麦等农作物品种SNP高通量身份鉴定技术体系的建立和指纹数据库的构建提供了有利的参考。

关键词：小麦 SNP 高通量身份鉴定模式

Gut microbiota-astrocyte axis: new insights into age-related cognitive decline

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Neural Regeneration Research 2025年第4期20卷 990-1008页

作者： Lan Zhang Jingge Wei Xilei Liu Dai Li Xiaoqi pang Fanglian Chen Hailong Cao Ping Lei Haihe Laboratory of Cell Ecosystem Department of GeriatricsTianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalNational Key Clinical SpecialtyTianjin Institute of Digestive DiseasesTianjin Key Laboratory of Digestive DiseasesTianjinChina Department of Urology Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institution Tianjin Medical University General HospitalTianjinChina

With the rapidly aging human population,age-related cognitive decline and dementia are becoming increasingly prevalent *** is considered the main risk factor for cognitive decline and acts through alterations in the composition of the gut microbiota,microbial metabolites,and the functions of *** microbiota–gut–brain axis has been the focus of multiple studies and is closely associated with cognitive *** article provides a comprehensive review of the specific changes that occur in the composition of the gut microbiota and microbial metabolites in older individuals and discusses how the aging of astrocytes and reactive astrocytosis are closely related to age-related cognitive decline and neurodegenerative *** article also summarizes the gut microbiota components that affect astrocyte function,mainly through the vagus nerve,immune responses,circadian rhythms,and microbial ***,this article summarizes the mechanism by which the gut microbiota–astrocyte axis plays a role in Alzheimer’s and Parkinson’s *** findings have revealed the critical role of the microbiota–astrocyte axis in age-related cognitive decline,aiding in a deeper understanding of potential gut microbiome-based adjuvant therapy strategies for this condition.

关键词： age aging Alzheimer’s disease astrocytes cognitive decline dementia gut microbiota gut–brain axis microbial metabolites neuroinflammation Parkinson’s disease

Overview of in-situ oxygen production technologies for lunar resources

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 233-255页

作者： Youpeng Xu Sheng pang Liangwei Cong Guoyu Qian Dong Wang Laishi Li Yusheng Wu Zhi Wang College of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China CAS Key Laboratory of Green Process and Engineering National Engineering Research Center for Green Recycling of Strategic Metal ResourcesInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China

The rich resources and unique environment of the Moon make it an ideal location for human expansion and the utilization of extraterrestrial ***,crucial for supporting human life on the Moon,can be extracted from lunar regolith,which is highly rich in oxygen and contains polymetallic *** oxygen and metal extraction can be achieved using existing metallurgical ***,the ample reserves of water ice on the Moon offer another means for oxygen *** paper offers a detailed overview of the leading technologies for achieving oxygen production on the Moon,drawing from an analysis of lunar resources and environmental *** delves into the principles,processes,advantages,and drawbacks of water-ice electrolysis,two-step oxygen production from lunar regolith,and one-step oxygen production from lunar *** two-step methods involve hydrogen reduction,carbothermal reduction,and hydrometallurgy,while the one-step methods encompass fluorination/chlorination,high-temperature decomposition,molten salt electrolysis,and molten regolith electrolysis(MOE).Following a thorough comparison of raw materials,equipment,technology,and economic viability,MOE is identified as the most promising approach for future in-situ oxygen production on the *** the corrosion characteristics of molten lunar regolith at high temperatures,along with the Moon's low-gravity environment,the development of inexpensive and stable inert anodes and electrolysis devices that can easily collect oxygen is critical for promoting MOE technology on the *** review significantly contributes to our understanding of in-situ oxygen production technologies on the Moon and supports upcoming lunar exploration initiatives.

关键词： lunar resources in-situ oxygen production space metallurgy molten lunar regolith electrolysis

我国75份小麦品种SNP和SSR指纹图谱构建与比较分析

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中国农业科技导报 2020年第5期22卷 15-23页

作者：刘丽华刘阳娜张明明李宏博庞斌双赵昌平北京市农林科学院北京杂交小麦工程技术研究中心杂交小麦分子遗传北京市重点实验室北京100097

联合利用Illumina 90K SNP芯片和毛细管高通量SSR检测技术,构建75份育成品种384个SNP和42个SSR位点的指纹图谱,比较两种标记在遗传多样性、遗传相似系数和鉴别能力等方面的特征。结果显示,SNP标记揭示的遗传多样性指数明显低于SSR标记,... 详细信息

联合利用Illumina 90K SNP芯片和毛细管高通量SSR检测技术,构建75份育成品种384个SNP和42个SSR位点的指纹图谱,比较两种标记在遗传多样性、遗传相似系数和鉴别能力等方面的特征。结果显示,SNP标记揭示的遗传多样性指数明显低于SSR标记,但能较好地反映品种间的遗传多样性;SNP标记揭示的遗传相似系数明显高于SSR标记,但两者呈极显著线性相关;384个SNP位点鉴定近等基因系的能力低于42个SSR位点,但去除近等基因系后,仅需8个SNP或4个SSR位点组合即可区分剩余的74份品种,表明最优位点组合具有较高的鉴定效率,在品种鉴定时可先采用少量标记进行初鉴,对于极近似品种可加大标记密度。首次结合SSR和SNP标记构建指纹图谱,证实了两者之间的一致性,提出了分子身份鉴定技术标记数量的选择思路,为小麦品种DNA身份鉴定技术标准制定提供了重要的参考依据。

关键词： SNP SSR 小麦品种指纹图谱