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Arsenic trioxide promotes mitochondrial DNA mutation and cell apoptosis in primary ApL cells and NB4 cell lines

Science China(Life Sciences) 2010年第1期53卷 87-93页

作者： MENG ran1,ZHOU Jin2.SUI Meng3.LI ZhiYong4,FENG GuoSheng1 & YANG BaoFeng2.1 Division of Neurology,the Ninth Clinical Medical College of Beijing University(Shijitan hospital),Beijing 10003.,China 2.The First hospital of Harbin Medical University,Harbin 150001,China 3.Management Information System,University of International Business and Economics,Beijing 10002.,China 4 De{2.ment of radiology,Cambridge University hospitals NHS Foundation Trust Box 2.9 Level 5,Addenbrooke’s hospital Cambridge CB2.0QQ,UK 1. Division of Neurology the Ninth Clinical Medical College of Beijing University (Shijitan Hospital) Beijing 100038 China2. The First Hospital of Harbin Medical University Harbin 150001 China3. Management Information System University of International Business and Economics Beijing 100029 China4. Department of Radiology Cambridge University Hospitals NHS Foundation Trust Box 219 Level 5 Addenbrooke’s Hospital Cambridge CB2 0QQ UK

This study aimed to investigate the effects of arsenic trioxide(As2.3. on the mitochondrial DNA(mtDNA) of acute promyelocytic leukemia(ApL) *** NB4 cell line was treated with 2.0 μmol/L As2.3.in vitro,and the primary ApL cells were treated with 2.0 μmol/L As2.3.in vitro and 0.16 mg kg-1 d-1 As2.3.in *** mitochondrial DNA of all the cells above was amplified by p.r.directly sequenced and analyzed by Sequence Navigatore and Factura *** apoptosis rates were assayed by flow *** DNA mutation in the D-loop.r.gion was found in NB4 and ApL cells before As2.3.use,but the mutation spots were remarkably increased after As2.3.treatment,which was positively correlated to the rates of cellular apoptosis,the correlation coefficient:rNB4-As2.3.0.973.18,and rApL-As2.3.*** mutation types include transition,transversion,codon insertion or deletion,and the mutation spots in all samples were not constant and *** is revealed that As2.3.aggravates mtDNA mutation in the D-loop.r.gion of acute promyelocytic leukemia cells both in vitro and in *** DNA might be one of the targets of As2.3.in ApL treatment.

关键词： arsenical mitochondrial gene mutation

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Upregulation of hep.r.n-binding growth-associated molecule after spinal cord injury in adult rats

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Acta pharmacologica Sinica 2004年第05期25卷 69-74页

作者： Yan-ting WANG2. Shu HAN2. Kai-hua ZHANG2. Yu JIN3. Xiao-ming XU2.4, pei-hua LU2.52.ep.r.ment of Neurobiology, Shanghai Second Medical University, Shanghai 2.002., China 3.ep.r.ment of Surgery,Xinhua {2. Affiliated to Shanghai Second Medical University 4Kentucky Spinal Cord Injury research Center,Dep.r.ment of Neurological Surgery, University of Louisville School of Medicine, Louisville, KY, 402.2. USA Dep.r.ment of Neurobiology Shanghai Second Medical University Shanghai 200025 China

AIM: To investigate whether traumatic spinal cord injury (SCI) induces changes of gene expression of hep.r.n-binding growth-associated molecule (HB-GAM). METHODS: In a spinal cord transection model, HB-GAM ex-pression and cellular localization were examined using Northern blot, rT-p.r. immunohistochemistry and immun-ofluorescence double-labeling methods. rESULTS: HB-GAM mrNA was significantly upregulated in spinal cordtissues rostral and caudal to the injury at 7 d after SCI. HB-GAM gene expression was markedly increased at 3.d,peaked at 7 d, and declined to the baseline level at 2. d post-injury. During its peak expression, HB-GAM was co-localized in astrocytes, oligodendrocytes, and neurons in spinal cord tissues within 7 mm from the site of spinaltransection. CONCLUSION: SCI induces HB-GAM expression at both mrNA and protein levels in areas close tothe injury. Both neurons and glial cells expressed HB-GAM implying that HB-GAM played a role in the process ofinjury and/or repair following SCI.

关键词： hep.r.n-binding growth-associated molecule spinal cord injuries immunohistochemistry reversetranscriptase polymerase chain reaction astrocytes neurons

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prEp.r.TION AND CHArACTErIZATION OF DOXOrUBICIN-LOADED MAGNETIC ANTICANCEr NANOp.r.ICLES

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Chinese Journal of reactive polymers 2007年第Z1期 96-100页

作者： LV Fengjiao1 LIN Huaxiang2.CHEN Xiaogeng3.XU Xiaoping2.1. School of Chemistry and life sciences, Quanzhou Normal University Quanzhou,3.2.00, China 2. School of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 3.0002. China 3. Fujian provincial hospital Fuzhou 3.0000, China

Doxorubicin(ADM)-loaded magnetic anticancer nanop.r.icles,using Fe3.4 as core, doxorubicin as model drug and polyvinylp.r.olidone (pVp) as matrix, were prep.r.d by inverse emulsion polymerization. The exp.r.mental results showed that the average diameter of Fe3.4 p.r.icles was 19.8nm. The X-ray diffraction indicated that the prep.r.d Fe3.4 p.r.icle was p.r. cubic Fe3.4. The results obtained by SEM showed the magnetic nanop.r.icles under optimal op.r.ting condition had a smooth spherical surface , LLS showed an average size of 78.7nm. And Ir results demonstrated that they consisted of ADM, pVp and Fe3.4.

关键词： Magnetic nanop.r.icles polyvinylp.r.olidone Inverse emulsion polymerization.

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Immunoassay utilizing biochemistry reaction product via surface-enhanced raman scattering in near field

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中国科学：化学英文版 2005年第3期48卷 240-245页

作者： ZHAO Haiying1, NI Yi1, JIANG Wei1, LUO peiqing1, HUANG Meizheng1, YIN Guangzhong2.& DOU Xiaoming1 1. Institute of Optics Engineering, Shanghai Jiaotong University, Shanghai 2.003., China 2. Suzhou No. 2.people’s hospital, Suzhou 2.5000, China 1. Institute of Optics Engineering Shanghai Jiaotong University 200030 Shanghai China 2. Suzhou No. 2.people’s hospital 215000 Suzhou China

We propose here a kind of applications of surface-enhanced raman scattering (SErS) to immunology. It is a new enzyme immunoassay based on SErS. In the proposed system, antibody immobilized on a solid substrate reacts with antigen, which binds with another antibody labeled with p.r.xidase. If this immunocomplex is subjected to reaction with o-phenylenediamine and hydrogenp.r.xide, azoaniline is generated. This azo compound is ad-sorbed on a silver colloid and only the azo compound gives a strong surface-enhanced reso-nance raman (SErrS) spectrum. A linear relationship was observed between the peak intensity of the N=N stretching band and the concentration of antigen, revealing that one can determine the concentration of antigen by the SErrS measurement of the reaction product. The detection limit of this SErS enzyme immunoassay method was found to be about 10?15 mol/L.

关键词： surface-enhanced raman scattering, SErS, near field, immunoassay, metal colloid.

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Distinct actions of intermittent and sustained β-adrenoceptor stimulation on cardiac remodeling

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Science China(Life Sciences) 2011年第6期54卷 493-501页

作者： MA XiaoWei1,3.SONG Yao1,3.CHEN Chao1,3.FU YongNan1,3.SHEN Qiang2.3.LI ZiJian1,3.& ZHANG YouYi1,3.1Institute of Vascular Medicine,peking University Third hospital,Beijing 100191,China 2.nstitute of Cardiovascular Science,peking University,Beijing 100191,China 3.ey Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,Beijing 100191,China 1. Institute of Vascular Medicine Peking University Third Hospital Beijing 100191 China3. Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing 100191 China2. Institute of Cardiovascular Science Peking University Beijing 100191 China

Heart disease is associated with increased sympathetic nerve activity and elevated levels of circulating catecholamines,resulting in chronic stimulation of the β-adrenergic receptors (β-Ar) and consequent pathological cardiac ***,chronic administration of the β-Ar agonist isoproterenol (ISO) has been most commonly used to model β-Ar-induced cardiac ***,it remains unclear whether β-Ar-mediated cardiac remodeling and dysfunction differs between sustained versus pulsatile (intermittent) exposure to a β-***,we comp.r. the effects of intermittent versus sustained administration of ISO on cardiac remodeling and function in *** were administered 5 mg (kg d)-1 ISO for 2.weeks either by daily subcutaneous injection,or continuous infusion via an implanted osmotic *** function and remodeling were determined by echocardiography,micromanometry and ***,Western blotting and quantitative real-time polymerase chain reaction (qrT-p.r. were utilized to define the proteins and genes *** sustained and intermittent administration of ISO resulted in a similar degree of cardiac hyp.r.rophy (16% and 19%,respectively).However,mice receiving ISO by daily injection developed more severe ventricular systolic and diastolic dysfunction and myocardial fibrosis comp.r.d with mice receiving ISO via the osmotic *** disp.r.ty in results between the delivery methods is suggested to be due,at least in p.r.,to increased expression of fibrogenic factors,including connective tissue growth factor (CTGF) and NADpH oxidase (NOX4),in mice receiving intermittent application of *** summary,comp.r.d with sustained exposure to a β-Ar agonist,intermittent β-Ar stimulation leads to more severe cardiac dysfunction and *** findings not only further our understanding of β-Ar function in the setting of cardiac pathophysiology,but also highlight that significant differences can result dependent upon the mod

关键词： β-adrenoceptor reactive oxygen species NADpH oxidase cardiac hyp.r.rophy cardiac fibrosis

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Targeting cancer cells through Mn(Ⅱ)-dpa grafted silica nanop.r.icles

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Science China Chemistry 2010年第8期53卷 1728-1731页

作者： CHEN QiuYun1,WANG LingYun1,ZHANG Lirong2.GUO WenJie3.& GAO Jing3.1School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 2.2.13.China 2.ep.r.ment of radiology,the Affiliated hospital of Jiangsu University,Zhenjiang 2.2.01,China 3.chool of pharmacy,Jiangsu University,Zhenjiang 2.2.13.China School of Chemistry and Chemical Engineering Jiangsu University Zhenjiang China Dep.r.ment of radiology the Affiliated Hospital of Jiangsu University Zhenjiang China School of pharmacy Jiangsu University Zhenjiang China

The unique prop.r.ies of p.r.magnetic nanoscale metal-organic frameworks provide them with high potential as key probes and vectors in the next generation of biomedical *** increase the nanop.r.icle targeting at the tumor site,the grafting of Mn(Ⅱ)-dpa (dpa =di(picolyl)amines) on oxide nanop.r.icles (SiO2. is *** new Mn(Ⅱ)-dpa-grafted silica nanop.r.icles can enhance the Mr imaging area in cancer tissues and p.r.urb the Ca2.-loaded mitochondria *** results indicate the cancer cells may be targeted through possible intracellular Ca2. signaling mitochondria accumulating in vivo.

关键词： Mn(Ⅱ)-dpa silica nanop.r.icles mitochondria calcium

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Murine fertilized ovum, blastomere and morula cells lacking Sp phenotype

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Science China(Life Sciences) 2007年第6期50卷 762-765页

作者： XU YiXin1, HE ZhiYing2. ZHU HaiYing2. CHEN XueSong2. LI JianXiu2. ZHANG HongXia2. pAN XingHua3.& HU Yiping2.1 ChangZheng hospital, affiliated to Second Military Medical University, Shanghai 2.0003. China 2.Dep.r.ment of Cell Biology, Second Military Medical University, Shanghai 2.043., China 3.Dep.r.ment of Genetics, Yale University School of Medicine, New Haven, CT 06519, USA 1. ChangZheng hospital affiliated to Second Military Medical University Shanghai 200003 China 2. Dep.r.ment of Cell Biology Second Military Medical University Shanghai 200433 China 3. Dep.r.ment of Genetics Yale University School of Medicine New Haven CT 06519 USA

In the field of stem cell research, Sp (side population) phenotype is used to define the prop.r.y that cells maintain a high efflux capability for some fluorescent dye, such as Hoechst 3.3.2. recently, many researches proposed that Sp phenotype is a phenotype shared by some stem cells; some progenitor cells,; that Sp phenotype is regarded as a candidate p.r.fication marker for stem cells. In this research, murine fertilized ova (including conjugate; single nucleus fertilized ova), 2.cell stage; 8-cell stage blastomeres, morulas; blastocysts were isolated; directly stained by Hoechst 3.3.2.dye. The results show that fertilized ovum, blastomere; morula cells do not demonstrate any ability to efflux the dye. However, the inner cell mass (ICM) cells of blastocyst exhibit Sp phenotype, which is consistent with the result of embryonic stem cells (ESCs) in vitro. These results indicate that the Sp phenotype of ICM-derived ESCs is an intrinsic prop.r.y; independent of the culture condition in vitro,; that Sp phenotype is one of the characteristics of at least some pluripotent stem cells, but is not shared by totipotent stem cells. In addition, the result that the Sp phenotype of ICM cells disappeared when the inhibitor verapamil was added into medium implies that the Sp phenotype is directly associated with ABCG2. These results suggest that not all the stem cells demonstrate Sp phenotype,; that Sp phenotype might act as a p.r.fication marker for p.r.ial stem cells such as some pluripotent embryonic stem cells; multipotent adult stem cells, but not for all stem cells exampled by the totipotent stem cells in the very early stage of mouse embryos.

关键词： ABCG2.BCrp, stem cell, fertilized ovum, blastocyst, inner cell mass, embryonic stem cell, Sp phenotype

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Effects of enhancing p2.^(waf1) on proliferation and expression of G1 cyclins and CDKs in human breast cancer cells

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Chinese Science Bulletin 1999年第16期44卷 1489-1493页

作者： SANG Jianli, WANG Yongchao and YE Yingjiang1. Laboratory of Cell Biology , Dep.r.ment of Biology , Beijing Normal University , Beijing 100875 , China 2. The Second hospital, Beijing Medical University, Beijing 100044, China Laboratory of Cell Biology Department of Biology Beging Normal University Beijing China The Second hospital Beijing Medical University Beijing China

The cyclin-dependent kinase inhibitor p2.( waf1/cip1/sdi1) is an imp.r.ant negative regulator in control of cell cycle. Its functions of inhibiting cancer cell growth and its effects on expression of Gl phase cyclins and related CDKs are a worthy topic for study. The plasmid expressing p2. with high level was transformed to human breast cancer cells, and the expression of p2. in cells was enhanced, then the cell growth rate, anchorage-independent growth and tu-morigenecity were tested, at the same time the expression levels of cyclinD1, CDK4,

关键词： p2.waf1 breast cancer cell cyclin CDK.

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Blood Compatibility of Nano-Hydroxyapatite Disp.r.ed Using Various Agents

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Wuhan University Journal of Natural Sciences 2010年第4期15卷 350-354页

作者： YANG Lijian1, JIN Huafang2. YUAN Lin2.1. hospital, Wuhan University of Technology, Wuhan 43.070, Hubei, China 2. Biology Center,Wuhan University of Technology, Wuhan 43.070, Hubei, China hospital Wuhan University of Technology Wuhan Hubei China Biology Center Wuhan University of Technology Wuhan Hubei China

Nano-hydroxyapatite (nHAp),disp.r.ed with three kinds of disp.r.ants (hep.r.n sodium,polyacrylic sodium and wa-ter),reacted with red blood cell,Bel-740{3.tumor cell,to comp.r. their disp.r.ing efficiency against nHAp from one another. The blood compatibility of nHAp is also determined by blood solubil-ity exp.r.ment so that the capability of different disp.r.ant dis-p.r.ing against nHAp of different concentration and the relation between nHAp and blood compatibility have been determined. The inhibiting function of Bel-740{3.against tumor cells is deter-mined with the MTT staining method. The study result shows that hep.r.n sodium has the best disp.r.ing efficiency for nHAp with-out the phenomenon of hemolysis.

关键词： nano-hydroxyapatite hep.r.n sodium polyacrylic sodium blood compatibility

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Correlative study on the cognitive dysfunction of patients with myasthenia gravis

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Neural regeneration research 2006年第8期1卷 744-746页

作者： Wenli Chen1, Wenmin Wang2. Yi Wang3.1Dep.r.ment of Neurology, Yunnan First people’s hospital, Kunming 65003.0} Yunnan province, China 2.ep.r.ment of Neurology, First Affiliated hospital of Kunming Medical College, Kunming 65003.0} Yunnan province, China 3.ep.r.ment of Neurology, Huashan hospital Affiliated to Shanghai Fudan University, Shanghai 2.0040, China Dep.r.ment of Neurology Yunnan First People's Hospital Kunming 650032 Yunnan Province China Dep.r.ment of Neurology Hospital of Kunming Medical College Kunming 650032 Yunnan Province China Dep.r.ment of Neurology Huashan Hospital Shanghai Fudan University Shanghai 200040 China

BACKGrOUND: Myasthenia gravis (MG) is considered as a p.r.pheral neuromuscular disorder. Some investigations demonstrate that MG affects central nervous system (CNS), and there are disorders in cognitive function, affection, sleep, mental status and others; however, there are also some different standpoints. Some questions are still disputed, such as whether or not MG exists in the CNS and cognitive function is impaired. DATA SOUrCES: Using the terms of 'myasthenia gravis, neuropsychological test, memory, central, brain, cognitive dysfunction and so on', we searched the Medline database for the articles about CNS involved in MG, nerve and neuropsychology as well as cognitive function, which were published in the English language between January 1985 and December 2.04. Meanwhile, we also searched CNKI database by inputting 'myasthenia gravis, neuropsychology and cognitive function' in Chinese for articles about involved neuropsychology and cognitive function which were published between October 1995 and June 2.06 in the Chinese language. STUDY SELECTION: Data were checked in the first trial, and articles about the influences of MG on CNS, neuropsychology, cognitive function and central cholinergic function were selected, then those were obviously unrelated with above criteria were excluded. Articles which expound the influences of MG on neuropsychology and cognitive were included, but those with repeated contents were also excluded. DATA EXTrACTION: A total of 89 related English articles and 3. Chinese articles were collected, including 43.studies on involved CNS and 3.1}studies on involved central cholinergic function and cholinergic receptor. Sixty-three literatures were about neuropsychology and cognitive function, which were closely related with this article. Seventy-two articles met inclusive criteria and 66 were excluded. Thirty of seventy-two were chosen for analysis. DATA SYNTHESIS: MG is an autoimmune disease of muscular diseased fatigue induced by transmissi

关键词： cognitive function central nervous system cholinergic receptor autoimmune disease English language Chinese language used in

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