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Comparison between the quality and function of sperm after semen processing with two different methods

Asian Journal of Andrology 2001年第2期3卷 125-130页

作者： m.E.Hammadeh p.m.zavos p.Rosenbaum W.Schmidt Dept. of Obs/Gyn University of Saarland 66421 Homburg/Saar GermanyAndrology Insitute of America and Kentucky Center for Reproductive Medicine & IVF Lexington KY USADept. of Obs/Gyn University of Saarland 66421 Homburg/Saar GermanyDept. of Obs/Gyn University of Saarland 66421 Homburg/Saar Germany

Aim: To compare the recovery rate of morphologically normal and chromatin condensed spermatozoa from native se-men samples using the Spermprep^(Tm) filtration columns and percoll gradient centrifugation and to determine the influenceof the two processing techniques on fertilization and pregnancy rates in an IVF-ET program. methods: Sixteen se-men samples obtained from patient's husband were included in this study. Each was divided into two aliquots. The firstaliquot was processed with Spermprep^(Tm) filtration columns and the second, percoll gradient centrifugation. Smears weremade before and after semen processing with both methods for the evaluation of chromatin condensation (chromomycineCmA3) as well as morphology (strict criteria) of spermatozoa. One hundred and seventy oocytes were retrieved fromthe patients and the oocytes from each patient were subdivided into two sets : one set was inseminated using spermatozoaprocessed with Spermprep^(Tm) and the other inseminated after semen processing with percoll gradient centrifugation. Re-sults: The percoll method yielded a significantly higher percentage of chromatin condensed (90.8 ±6.5% vs 82.3±8.8%, p = 0.017) and morphologically normal spermatozoa (12.9±7.4% vs 6.9±4.8%, p =0.001) in com-parison to Spermprep^(Tm). Whereas, sperm count recovery rate was significantly higher after the use of Spermprep^(Tm) thanafter the percoll gradient centrifugation. The fertilization rate was similar between the two methods. Conclusion:Semen processing with percoll should be recommended for intracytoplasmic sperm injection as the natural selection isbypassed and the Spermprep^(Tm) technique could be recommended for IVF and IUI programs as the sperm concentrationplays a more significant role in these procedures.

关键词： sperm semen processing spermprep^(Tm) percoll gradient centrifugation IVF

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用免疫学方法分离X精子Y精子

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国外畜牧科技 1989年第5期16卷 14-18页

作者： p.m.zavos 周鼎年美国肯塔基大学

前言当今很多研究者在寻求分离X精子、Y精子的方法,以改变家畜后代的自然性比例。奶牛业希望多产母犊。人工授精站如果能改变后代性比,种公牛的选配方案会变得更有效。而一般奶牛场,如果所产犊全为雌性,则可将目前对母牛某性状的选择压... 详细信息

前言当今很多研究者在寻求分离X精子、Y精子的方法,以改变家畜后代的自然性比例。奶牛业希望多产母犊。人工授精站如果能改变后代性比,种公牛的选配方案会变得更有效。而一般奶牛场,如果所产犊全为雌性,则可将目前对母牛某性状的选择压提高一倍。马匹用性别分离的精液进行选配,可以选择符合市场需要的性比。在肉畜(肉牛、猪、羊)中,性别选择可使基础群多生母仔,遗传性较差的母畜只产生公仔,从而最终淘汰这些品系。在肉牛中,可通过性控使第一次杂交产母犊,第二次杂交(终端杂交)产公犊,以利发挥杂种优势。

关键词：家畜 X精子 Y精子分离免疫学方法

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阴道内注入H-Y抗血清确定家兔性别

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草食家畜 1984年第5期 46-47页

作者： p.m.zavos 刘铁铮周光恒

Eichwald等报告过雄性小鼠的皮肤同基因移植片常为雌鼠所排斥。这表明存在着一种受Y染色体影响的抗原。由于该抗原与细胞介导的免疫应答有关,故称为小鼠的H-Y抗原(histocompatibility-Y)。有人用补体介导的细胞毒性试验检出了小鼠精子的... 详细信息

Eichwald等报告过雄性小鼠的皮肤同基因移植片常为雌鼠所排斥。这表明存在着一种受Y染色体影响的抗原。由于该抗原与细胞介导的免疫应答有关,故称为小鼠的H-Y抗原(histocompatibility-Y)。有人用补体介导的细胞毒性试验检出了小鼠精子的H-Y抗原。免疫电镜的研究发现,H-Y抗原集中在小鼠精子的顶体部位。从C57BL/6J小鼠附睾和输精管收集的精子,经H-Y抗血清和马的补体处理后再行人工授精,结果显著地提高了雌性小鼠产出数。

关键词：抗血清小鼠精子抗原母兔 H-Y 精子活力精子活率对照血清阴道内性别

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Influence of cerium ions on structure-dependent magnetic properties of Ba-Sr m-type hexaferrite nanocrystals

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Journal of Rare Earths 2024年第2期42卷 364-372,I0005页

作者： Ram A.pawar Sunil m.patange p.m.Dighe Sher Singh meena Abdullah GAl-Sehemi mehboobali pannipara Santosh S.Jadhav Department of physics ArtsCommerce and Science CollegeAhmednagar413711MaharashtraIndia Department of physics Shri Krishna MahavidyalayaGunjotiDistOsmanabad413606MaharashtraIndia padmashri Vikhe patil College of Arts Science and CommercePravaranagarA/P.Loni(Kd)Tal.RahataDistAhmednagar413713MaharashtraIndia Solid State physics Division Bhabha Atomic Research CentreMumbai400085MaharashtraIndia Department of Chemistry King Khalid UniversityAbha61413Saudi Arabia Department of physics D.S.M's ArtsCommerce and Science CollegeJintur431509MaharashtraIndia

The present study is a systematic effort to investigate the structure-sensitive magnetic parameters of Ce^(3+) substituted Ba-Sr hexaferrite nanocrystals chemically formulated as Ba_(0.5)Sr_(0.5)Ce_(x)Fe_(12-x)O_(19) where x=0.0-0.2 with Δx=*** hexaferrite powders were prepared using the sol-gel self-ignition route and structurally characterized by means of powder X-ray diffraction and Fourier transform infrared *** creation of the m-type hexaferrite phase within the synthesized samples was revealed from the Rietveld refinement of the X-ray *** occurrence of a secondary phase of CeO_(2) was revealed within the hexaferrites for the substitution,x> *** refined X-ray diffraction data were utilized to compute the lattice parameters,X-ray density,and lattice parameter *** crystal structure plotted from the refined XRD data reveals the occupancy of the ions at different lattice *** XpS data of the hexaferrite were analyzed to confirm the oxidation states of the constituent *** nanocrystalline nature of the hexaferrites was revealed from the crystallite sizes calculated using Scherer's *** analysis of FTIR spectra confirms that only a fraction of Ce^(3+)accommodated in the lattice and the remaining Ce content reside in the form of the CeO_(2) *** morphology of the hexaferrites was analyzed from the FESEm profiles of the ferrite *** magnetic behavior study was performed by analyzing the Curie temperature,hysteresis loops,and hyperfine interactions by means of susceptibility,V.S.m,and mossbauer spectroscopy,*** hexaferrites with increasing coercivity,decreasing saturation magnetization,and decreasing Curie temperature are reported in the *** substituted Ce^(3+)ions inhibit the grain growth and create lattice imperfections giving rise to hexaferrites with tuned magnetic parameters suitable for different applications.

关键词： Ba-Sr hexaferrite Ce^(3+)substitution XpS Saturation magnetization Coercivity m?ssbauer spectroscopy

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Intertype superconductivity evoked by the interplay of disorder and multiple bands

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Frontiers of physics 2024年第4期19卷 81-89页

作者： p.m.marychev A.A.Shanenko A.V.Vagov HSE University 101000 MoscowRussia Center for Advanced mesoscience and Nanotechnology MIPTDolgoprudny141700Russia

Nonmagnetic impurity scattering is known to shift up the Ginzburg-Landau parameter k of a *** this case,when the system is initially in type I,it can change its magnetic response,crossing the intertype domain with k~1 between the two standard superconductivity types and arriving at type *** the present work we demonstrate that the impact of disorder can be much more profound in the presence of the multiband structure of the charge carrier *** particular,when the band diffusivities differ from each other,the intertype domain tends to expand significantly,including points with k>1 that belong to deep type-Ⅱ in conventional single-band *** finding sheds light on the nontrivial disorder effect and significantly complements earlier results on the enlargement of the intertype domain in clean multiband superconductors.

关键词： superconductivity disorder intertype superconductivity twoband model

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mechanisms behind the environmental sensitivity of carbon fiber reinforced polytetrafluoroethylene(pTFE)

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Friction 2024年第5期12卷 997-1015页

作者： pontus JOHANSSON p.mARKLUND m.BJORLING Y.SHI Division of machine Element LuleåUniversity of TechnologyLuleåSE-97187Sweden

Carbon fiber reinforced polytetrafluoroethylene (CF/pTFE) composites are known for their exceptional tribological performance when sliding against steel or cast iron in inert gas environments. Compared to experiments in humid air, about an order of magnitude lower wear rate and several times lower coefficient of friction have been reported for tests conducted in dry nitrogen and hydrogen. moreover, trace moisture has been shown to affect the friction and wear significantly of this tribosystem, although a possible effect of oxygen cannot be ruled out due to uncertainties regarding the oxygen concentrations. While several studies have pointed out the environmental sensitivity of CF/pTFE, the understanding of the underlying mechanisms are very limited. The objective of this research is to investigate the individual and combined effect of oxygen and moisture on the tribological behavior of CF/pTFE sliding against steel. Additionally, this study aims to elucidate the underlying mechanisms that govern the environmental sensitivity of the system. Climate-controlled three-pin-on-disc experiments were conducted in nitrogen atmospheres at various concentrations of oxygen and moisture. The tribological results clearly demonstrate that both moisture and oxygen contribute to increased friction and wear. However, the adverse effect was much more pronounced for oxygen than moisture. A qualitative method was developed to estimate the tribofilm coverage on the CF/pTFE surface. Results showed strong correlation between high coverage of strongly adhered tribofilm and low wear rate. moreover, a loosely adhered tribofilm was observed on top of the CF/pTFE surface in presence of moisture. FTIR analysis indicated that the loosely adhered tribofilm found in the moisture-enriched environment contained a significant amount of adsorbed water, which may explain the lower coefficient of friction in presence of moisture compared to oxygen. The adsorbed water in the loosely adhered tribofilm coul

关键词： polymer composite tribofilm tribochemistry atmosphere

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Drought events influence nutrient canopy exchanges and green leaf partitioning during senescence in a deciduous forest

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Forest Ecosystems 2024年第1期11卷 110-119页

作者： J.Touche C.Calvaruso p.De Donato m.-p.Turpault INRAE BEFF-54000NancyFrance UmR 7359 GeoRessourcesFaculte des Sciences-Campus des Aiguillettes54506Vandoeuvre Les NancyFrance EcoSustain Environmental Engineering OfficeResearch and DevelopmentKanfen57330France

The increase in the frequency and intensity of drought events expected in the coming decades in Western Europe may disturb forest biogeochemical cycles and create nutrient deficiencies in *** possible origin of nutrient deficiency is the disturbance of the partitioning of the green leaf pool during the leaf senescence period between resorption,foliar leaching and senesced ***,the effects of drought events on this partitioning and the consequences for the maintenance of tree nutrition are poorly *** experiment in a beech forest in meuse(France)was conducted to assess the effect of drought events on nutrient canopy exchanges and on the partitioning of the green leaf pool during the leaf senescence *** aim was to identify potential nutritional consequences of droughts for *** nutrient dynamics,including resorption,chemistry of green and senesced leaves,foliar absorption and leaching in mature beech stands from 2012 to 2019 allowed us to compare the nutrient exchanges for three nondry and three dry years(i.e.,with an intense drought event during the growing season).During dry years,we observed a decrease by almost a third of the potassium(K)partitioning to resorption(*** efficiency),thus reducing the K reserve in trees for the next growing *** result suggests that with the increased drought frequency and intensity expected for the coming decades,there will be a risk of potassium deficiency in trees,as already observed in a rainfall exclusion experiment on the same study *** foliar leaching and higher parititioning to the senesced leaves for K and phosphorus(p)were also *** addition,a slight increase in nitrogen(N)resorption efficiency occurred during dry years which is more likely to improve tree *** calcium(Ca)negative resorption decreased,with no apparent consequence in our study *** results show that nutrient exchanges in the canopy and the partitioning of the green leaf

关键词： Natural drought Beech forest Canopy Resorption Foliar leaching Senesced leaves Nutrition

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Tensile deformation of fine-grained mg at 4K,78K and 298K

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Journal of magnesium and Alloys 2024年第6期12卷 2534-2556页

作者： m.Walag A.Kula p.Noga T.Tokarski G.Cios m.Niewczas AGH University of Krakow Faculty of Non-Ferrous MetalsKrakowPoland AGH University of Krakow Academic Centre for Materials and NanotechnologyKrakowPoland materials Science and Engineering McMaster UniversityHamiltonCanada

The impact of grain size,ranging from 0.9μm to 9μm,on the mechanical properties of commercially pure mg is investigated at temperatures of 4K,78K,and *** mechanisms governing plastic flow are influenced by both grain size and *** 4K and 78K,dominant deformation modes in mg involve dislocation glide and extension twinning,regardless of grain *** interactions between basal and non-basal dislocations and dislocations with grain boundaries promote an unusually high rate of work hardening in the plastic regime,leading to premature *** yield stress follows the Hall-petch relationshipσ_(y)∼k/√d,with the slope k increasing with decreasing *** 298K,in addition to dislocation glide and twinning,grain boundary sliding(GBS)becomes significant in samples with grain sizes below 3μm,considerably enhancing the material’s *** activation provides an additional recovery mechanism for dislocations accumulating at grain boundaries,facilitating their absorption during sliding and *** ofσrelationship suggests that the basal slip is the dominant dislocation mode in mg at *** grain size suppresses dislocation activity and twinning and increases GBS,resulting in lowerandσ*** conventional deformation modes coupled with enhanced GBS yields stress softening,breaking down the Hall-petch relationship in mg below 3μm grain size,leading to an inverse Hall-petch *** work reports new data on the strength,ductility,work hardening and fracture behaviour,and their variations with mg grain size across different temperature regimes.

关键词： polycrystalline mg mechanical properties Deformation mechanisms Work-hardening Texture evolution Cryogenic temperatures Hall-petch relationship

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Consumer transparency in the production chain for plant varieties produced using new genomic techniques

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aBIOTECH 2024年第2期5卷 239-246页

作者： J.m.Lukasiewicz C.C.m.van de Wiel L.A.p.Lotz m.J.m.Smulders Wageningen University&Research P.O.Box 386NL-6700 AJ WageningenThe Netherlands

plants edited with new genomic techniques(NGTs)currently fall under the Genetically modified Organisms Directive(2001/18/EC)in the European *** the proposal of the European Commission,NGT plants are partially exempted from the regulations of this *** proposal makes a distinction between two categories of NGT plants:NGT-1 and ***-1 category plants are considered equal to plants obtained through conventional breeding *** plants will not be labelled for the consumer,although they will be labelled as ***-2 category plants may be labelled with additional information as a positive *** of seeds of varieties made with gene editing,but not the products,would mean that most steps in the production chain are transparent,but not the last step towards ***“right to know”and increasing knowledge of gene-edited food is a common theme in food labelling towards ***,we describe current labelling regimes and registers and how these may be applied to provide transparency on gene-edited products to ***,we also look into consumer studies,which indicate a greater acceptance of gene-edited food among consumers,especially when additional benefits such as sustainability are mentioned.

关键词： Labelling New genomic techniques Consumer transparency plant products

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Exact solutions for magnetohydrodynamic nanofluids flow and heat transfer over a permeable axisymmetric radially stretching/shrinking sheet

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Chinese physics B 2024年第2期33卷 108-114页

作者： U.S.mahabaleshwar G.p.Vanitha L.m.pérez Emad H.Aly I.pop Department of Studies in mathematics Davangere UniversityShivagangothriDavangereIndia Departamento de F´ısica FACIUniversidad de Tarapac´aCasilla 7DAricaChile Department of mathematics Faculty of EducationAin Shams UniversityRoxyCairoEgypt Department of mathematics Babes-Bolyai University400084 Cluj-NapocaRomania

We report on the magnetohydrodynamic impact on the axisymmetric flow of Al_(2)O_(3)/Cu nanoparticles suspended in H_(2)O past a stretched/shrinked *** the use of partial differential equations and the corresponding thermophysical characteristics of nanoparticles,the physical flow process is *** resultant nonlinear system of partial differential equations is converted into a system of ordinary differential equations using the suitable similarity *** transformed differential equations are solved *** of the magnetic parameter,solid volume fraction and stretching/shrinking parameter on momentum and temperature distribution have been analyzed and interpreted *** skin friction and Nusselt number were also *** addition,existence of dual solution was deduced for the shrinking sheet and unique solution for the stretching ***,Al_(2)O_(3)/H_(2)O nanofluid flow has better thermal conductivity on comparing with Cu/H_(2)O ***,it was found that the first solutions of the stream are stable and physically realizable,whereas those of the second ones are unstable.

关键词： magnetohydrodynamic nanofluid stretching/shrinking sheet axisymmetric flow analytical solution suction/injection

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