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Characterization of the tunicamycin gene cluster unveiling unique steps involved in its biosynthesis

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protein & Cell 2010年第12期1卷 1093-1105页

作者： Wenqing Chen Dongjing Qu Lipeng Zhai Meifeng Tao Yemin Wang Shuangjun Lin Neil p.J.price Zixin Deng Laboratory of Microbial Metabolism and School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai 200030China National Center for Agricultural Utilization Research(NCAUR) USDA-ARS1815 North University StreetPeoriaIllinois 61604USA College of pharmacy Wuhan UniversityWuhan 430072China

Tunicamycin,a potent reversible translocase I inhibitor,is produced by several Actinomycetes *** tunicamycin structure is highly unusual,and contains an 11-carbon dialdose sugar and anα,β-1″,11′-glycosidic *** we report the identification of a gene cluster essential for tunicamycin biosynthesis by high-throughput heterologous expression(HHE)strategy combined with a *** of the genes into heterologous non-producing Streptomyces hosts results in production of tunicamycin by these strains,demonstrating the role of the genes for the biosynthesis of *** disruption experiments coupled with bioinformatic analysis revealed that the tunicamycin gene cluster is minimally composed of 12 genes(tunA–tunL).Amongst these is a putative radical SAM enzyme(Tun B)with a potentially unique role in biosynthetic carbon-carbon bond ***,a seven-step novel pathway is proposed for tunicamycin ***,two gene clusters for the potential biosynthesis of tunicamycin-like antibiotics were also identified in Streptomyces clavuligerus ATCC 27064 and Actinosynnema mirums DSM *** data provide clarification of the novel mechanisms for tunicamycin biosynthesis,and for the generation of new-designer tunicamycin analogs with selective/enhanced bioactivity via combinatorial biosynthesis strategies.

关键词： tunicamycin biosynthetic gene cluster high-throughput heterologous expression bioassay combinatorial biosynthesis

Interleukin-1 gene polymorphism disease activity and bone mineral metabolism in rheumatoid arthritis

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Chinese Medical Journal 2002年第1期115卷 46-49,145页

作者：张晓中 L Llamado I pillay p price R Will 山医科大学第三附属医院风湿科广州510630

Objective To determine whether interleukin-1α and 1β gene polymorphism is associated with rheumatoid arthritis disease activity and bone mineral metabolism, and whether there is any relationship between IL-1β and rheumatoid arthritis (RA) motif gene. Methods IL-1 gene polymorphisms were analyzed in 65 RA patients who met American College of Radiology (ACR) criteria and 60 controls. From genomic DNA, 2 polymorphisms in each gene for IL1α-889 and IL-1β+3953 were typed by pCR-RFLp and HLA-DRB1 allele typing was also undertaken by pCR-SSOp. Some clinical and laboratory parameters were collected. The allelic frequencies and carriage rates were compared between RA patients and controls and between patients with active and quiescent disease. Comparison was also made between IL-1 polymorphism and parameters of bone mineral metabolism and between patients with the HLA-DRB1 RA motif plus IL-1β 2 and patients without the two alleles. Fisher test and the analysis of variance was used to analyze the *** There was no significant difference in the frequency and carriage rate of IL-1α polymorphisms between RA patients and the controls. The β2/2 genotype of IL-1β was more common in female RA patients compared with controls (p=0.001). A lower carriage rate of IL-1β 2 occurred in male RA patients (p=0.001). A higher carriage rate of IL-1α2 is associated with a higher ESR (p=0.008), HAQ score (p=0.03), and vit-D 3 (pp=0.002), a lower RF (p=0.002) and a lower BMD at the lumbar spine (p=0.001). A higher frequency of IL-1α1 is associated with a lower CRp value (p=0.009). An increased IL-1β2 carriage is associated with active rheumatoid disease as indicated by a higher CRp (pppain score (p=0.001) and a higher BMD at the lumbar spine (p=0.007), lower vit-D 3 and. Udpd/Crea level The presence of the HLA DRB1 RA motif and IL-1β allele 2 at same time did not contribute to disease ***

关键词： rheumatoid arthritis · interleukin-1 · gene polymorphism · disease activity · bone mineral metabolism

Diagnostic technology:alternative sampling methods for collection of urine specimens in older adults

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Family Medicine and Community Health 2013年第2期1卷 43-49页

作者： KATRIEN LATOUR ANNETTE pLÜDDEMANN MATTHEW THOMpSON BOUDEWIJN CATRY CHRISTOpHER p price CARL HENEGHAN FRANK BUNTINX University of Leuven Belgium

Clinical Question Which sampling methods other than in-and-out catheterization or suprapubic aspiration can be used to collect valid uncontaminated urine specimens for microbiological culture in older adults?Background,Current practice and Advan-tages over Existing Technology Urinary tract infections(UTIs)are one of the most common bacterial infections in older *** increasing research into this infectious disease,substantial uncertainty still surrounds its *** tend to be less specific in frail elderly and difficulties with cognition or communication can further impair clinical evaluation[1,2].Consequently,physi-cians often resort to urinary analysis to support the diagnosis.

关键词： urine urinary resort

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河流数学模型

水道港口 1984年第3期 42-46页

作者： p K price 汤卫

一、模型结构一个河流数学模型有三个主要部分。(1)一组方程,用以从各种途径描述水流;(2)河段的特征资料;(3)给定资料的方程求解方法。一维渐变流方程的简化形式如下: 连续方程

关键词：方程稳定流复式断面修正系数数学模型试验模型河漫滩泛滥平原冲积平原

Acetabular cup size trends in total hip arthroplasty

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World Journal of Orthopedics 2024年第1期15卷 39-44页

作者： Daniel patrick McKenna Alex price Timothy McAleese Darren Dahly paul McKenna May Cleary Department of Trauma and Orthopaedics University Hospital WaterfordWaterford X91 ER8EIreland Department of Epidemiology and public Health University College CorkCork T12 XF62Ireland

BACKGROUND Total hip arthroplasty(THA)is a common procedure for end stage *** learning curve for THA is complex and *** of the most difficult skills to master is acetabular *** wish to identify if experience in arthroplasty leads to preservation of more bone *** To investigate if increasing surgeon experience will predict an ever decreasing acetabular cup *** A retrospective case series of four attending orthopaedic surgeons was *** uncemented elective total hip arthroplasties since appointment were selected for *** size of acetabular cup used was noted and logistic regression was used to identify if a trend to smaller cups *** A total of 1614 subjects were included with a mean age of 64 *** cups were on average 0.18mm smaller per year(95%confidence interval-0.25 to-0.11,pp sizes to decrease 0.27 mm/year for surgeon A,0.02 mm/year for surgeon B,0.15 mm/year for surgeon C and 0.29 mm/year for surgeon *** of the four surgeons had a more pronounced trend to smaller cups for male subjects than their female *** We found increasing surgeon experience to be associated with an ever-decreasing acetabular cup *** acetabular cup size may act as a surrogate marker of surgical proficiency by virtue of decreased acetabular reaming.

关键词： Hip Arthroplasty Acetabulum Cup Learning

巴西亚马孙流域热带森林的开垦与大豆生产:以朗多尼亚州维列纳市为例

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AMBIO－人类环境杂志 2005年第6期34卷 456-463页

作者： J.ChristopherBrown Matthew Koeppe Benjamin Coles Kevin p.price 潘韬堪萨斯大学地理与环境工程研究系堪萨斯大学堪萨斯大学地理系

近来巴西大豆生产的增长引发了环境保护团体以及一些学者对于亚马孙盆地的热带自然环境正在向大豆种植地转换的关注。大豆生产的支持者则认为大豆是区域的一种可行性农产品;对于环境的关注是没有根据的,因为新的大豆种植地正在替代已经... 详细信息

近来巴西大豆生产的增长引发了环境保护团体以及一些学者对于亚马孙盆地的热带自然环境正在向大豆种植地转换的关注。大豆生产的支持者则认为大豆是区域的一种可行性农产品;对于环境的关注是没有根据的,因为新的大豆种植地正在替代已经被砍伐的林地或者是转换了的土地。两种观点虽被提出却都没有对大豆生产扩充区的土地利用与土地覆被(LULC)状况进行过综合研究与估量。本案例研究是在巴西的朗多尼亚州,亚马孙盆地西南部的维列纳市(Vilhena),通过遥感手段对1996～2001年以来该市大豆种植面积的大幅度增加所伴随的土地利用与土地覆被变化状况进行评估。结果表明虽然森林变成了大豆生产基地,但是大部分的大豆生产的增长归功于对已有土地的微量扩充、已伐林地的转换以及更高的单位产量。

关键词：大豆生产热带森林亚州土地覆被变化开垦流域西亚自然环境土地利用环境保护

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用遥感和GIS技术评价留用地的土壤特性

水土保持科技情报 1999年第1期17卷 10-13页

作者： J.Wu M.D.Nellis M.D.Ransom K.p.price S.L.Egbert 马宗兆杞杰山西林校

美国国会确定留用地保护项目（CRp）的最初国的是为减少农用地边缘的土壤侵蚀。全美目前有2.43×1.06hm2农田登记纳入该项计划。对该项目减少侵蚀的有效性评价将对其令后的实施修订有很大应用价值。在堪萨斯州Finney县利用卫星图像导出... 详细信息

美国国会确定留用地保护项目（CRp）的最初国的是为减少农用地边缘的土壤侵蚀。全美目前有2.43×1.06hm2农田登记纳入该项计划。对该项目减少侵蚀的有效性评价将对其令后的实施修订有很大应用价值。在堪萨斯州Finney县利用卫星图像导出土壤使用和覆差情况图。通过地理信息系统（GIS）将该图与计算机编程的土壤探测信息（SSURGO）结合，对每种利用地块类型（去除了该县的最高侵蚀系数和最易受侵蚀的地块）土壤特性和侵蚀系数进行分析，该研究表明CIS和遥感技术对评价和监测该留用地保护计划有许多优点。

关键词：留用地保护计划侵蚀系数 GIS 遥感土壤特性

performance of Bt-susceptible and -heterozygous dual-gene resistant genotypes of Spodoptera frugiperda (***) (Lepidoptera:Noctuidae) in seed blends of non-Bt and pyramided Bt maize

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Insect Science 2021年第4期28卷 1147-1158页

作者： Marcelo Dimase Sebe Brown Graham p.Head paula A.price Wade Walker Wenbo Yu Fangneng Huang Department of Entomology Louisiana State University Agricultural CenterBaton RougeLouisianaUSA Dean Lee Research Station Louisiana State University Agricultural CenterAlexandriaLouisianaUSA Bayer Crop Science ChesterfieldMissouriUSA Macon Ridge Research Station Louisiana State University Agricultural CenterWinnsboroLouisianaUSA

A seed blend refuge has been implemented in the *** Belt for Bt maize resistance *** fall armyworm,Spodoptera frugiperda(***),is a target pest of Bt maize in the *** larvae of this pest are mobile,which may affect the efficacy of seed blend *** this study,field and greenhouse trials were conducted to determine the performance of Bt-susceptible(aabb)and-heterozygous dual-gene-resistant(AaBb)genotypes of *** in seed blends of non-Bt and pyramided Bt *** field trials evaluated larval survival,larval growth,and plant injury with aabb in seed blends of Bt maize expressing Cry1A.105/Cry2Ab2/Vip3A with 0–30%non-Bt *** tests investigated the performance of aabb and AaBb in seed blends of Cry1A.105/Cry2Ab2 with 0–30% non-Bt *** pure non-Bt maize plots,after 9–13 d of neonates being released on the plants,0.39 and 0.65 larvae/plant survived with leaf injury ratings of 4.7 and 5.9(Davis's 1–9 scale)in the field and greenhouse,*** contrast,live larvae and plant injury were virtually not observed on Bt plants across all planting *** occurrence and plant injury by aabb on non-Bt plants were similar between seed blends and pure non-Bt plantings,suggesting that the blended refuges could provide an equivalent susceptible population as structured refuge under the test *** the greenhouse,the two insect genotypes in seed blends performed similarly,indicating that the seed blends did not provide more favorable conditions for AaBb over *** information generated from this study should be useful in managing *** and evaluating if send blends could be suitable refuge options for Bt resistance management in the regions where the insect is a primary target pest.

关键词： Bt maize dual-gene resistance fall armyworm resistance management seed blend

Levels of CMV-reactive antibodies correlate with the induction of CD28^null T cells and systemic inflammation in chronic obstructive pulmonary disease （COpD）

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Cellular & Molecular Immunology 2016年第4期13卷 551-553页

作者： Dino BA Tan Fathiah S Amran Teck-Hui Teo patricia price Yuben p Moodley Centre for Asthma Allergy and Respiratory Research School of Medicine& Pharmacology University of Western Australia Nedlands WA Australia Stem Cell Unit Lung Institute of WA University of Western Australia Nedlands WA Australia School of pathology and Laboratory Medicine University of Western Australia Nedlands WA Australia School of Biomedical Science Curtin University Bentley WA Australia Department of Respiratory and Sleep Medicine Royal Perth Hospital Perth WA Australia

Chronic obstructive pulmonary disease （COpD） is characterized by chronic irreversible inflammation and progressive decline in lung function. While smoking and other environmental agents （e.g., air pollutants） are common causes of COpD, the major challenges in managing this condition are the persistent inflammation and functional deterioration which are not reversed by removal of precipitating agents （e.g., smoking cessation） or treatment with anti-inflammatory agents.

关键词：肺肺疾病慢性阻塞性戒烟巨细胞病毒抗原 CD28 吸烟消炎药炎症