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Optimization of chemistry and process parameters for control of intermetallic formation in Mg sludges

Journal of Magnesium and Alloys 2024年第4期12卷 1431-1448页

作者： Y.Fu G.G.Wang A.Hu Y.Li k.B.Thacker J.p.Weiler H.Hu Department of Mechanical Automotive and Materials EngineeringUniversity of Windsor401 Sunset AveWindsorOntario N9B 3P4Canada Global Technology Centre Meridian Lightweight Technologies25 MacNab AveStrathroyOntario N7G 4H6Canada

Intermetallic formation in sludge during magnesium(Mg)melting,holding and high pressure die casting practices is a very important ***,very often it is overlooked by academia,original equipment manufacturers(OEM),metal ingot producers and even die *** aim of this study was to minimize the intermetallic formation in Mg sludge via the optimization of the chemistry and process *** Al8Mn5 intermetallic particles were identified by the microstructure analysis based on the Al and Mn *** design of experiment(DOE)technique,Taguchi method,was employed to minimize the intermetallic formation in the sludge of Mg alloys with various chemical compositions of Al,Mn,Fe,and different process parameters,holding temperature and holding *** sludge yield(SY)and intermetallic size(IS)was selected as two *** optimum combination of the levels in terms of minimizing the intermetallic formation were 9 wt.%Al,0.15 wt.%Mn,0.001 wt.%(10 ppm)Fe,690℃ for the holding temperature and holding at 30 mins for the holding time,*** best combination for smallest intermetallic size were 9 wt.%Al,0.15 wt.%Mn,0.001 wt.%(10 ppm)Fe,630℃ for the holding temperature and holding at 60 mins for the holding time,*** groups of sludge factors,Chemical Sludge(CSF),physical Sludge(pSF)and Comprehensive Sludge Factors(and CpSF)were established for prediction of sludge yields and intermetallic sizes in Al-containing Mg *** CpSF with five independent variables including both chemical elements and process parameters gave high accuracy in prediction,as the prediction of the pSF with only the two processing parameters of the melt holding temperature and time showed a relatively large deviation from the experimental *** Chemical Sludge Factor was primarily designed for small ingot producers and die casters with a limited melting and holding capacity,of which process parameters could be fixed *** physical Sludge Factor could be used f

关键词： Magnesium sludge Al-Mn intermetallic Optimization Taguchi method Sludge factor Chemical composition process parameter

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Organic-inorganic hybrid materials and architectures in optoelectronic devices: Recent advancements

ChemPhysMater

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ChemphysMater 2024年第3期3卷 252-272页

作者： M.Arya S.Heera p.Meenu k.G.Deepa Department of physics University of KeralaThiruvananthapuram 695581India Department of Nanoscience and Nanotechnology University of KeralaThiruvananthapuram 695581India

Organic-inorganic hybrids are next-generation materials for use in high-performance optoelectronic devices owing to their adaptabilities in terms of design and *** article reviews the application of hybrid materials and layers in several widely used optoelectronic devices,i.e.,light amplification by stimulated emission of radiation(LASER),solar cells,and light-emitting diodes(LEDs).The effects of the incorporation of inorganic particles on photostability and optical gain are analyzed in the first section with reference to dye and perovskite ***,the strategies used in blending inorganic nanostructures into organic solar cells and bulk heterojunctions are *** use of various organic layers as electron-and hole-transport materials in Si heterojunction solar cells is reviewed in ***,the benefits of the presence of organic components in quantum-dot-and perovskite-based LEDs are derived from the *** integration of organic and inorganic components with optimal interfaces and morphologies is a challenge in developing hybrid materials with improved efficiencies.

关键词： Optoelectronics Hybrid material Device performance photostability Advantages

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Unique anti-angiogenic effects,pharmacological targets and therapeutic mechanisms of Chinese herbal medicines for endometriosis

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Genes & Diseases 2024年第5期11卷 63-65页

作者： Bo Liang Rui Dong Sze Wan Hung Yiran Li Yuezhen Lin Ling Wu Tao Zhang Gene Chi wai Man Hui Xu Jacqueline pui Wah Chung Chi Chiu Wang Guangdong provincial key Laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantouGuangdong 515063China Department of Obstetrics and Gynaecology The Chinese University of Hong KongHong Kong SARChina Reproduction and Development Laboratory Li Ka Shing Institute of Health SciencesThe Chinese University of Hong KongHong Kong SARChina School of Biomedical Sciences The Chinese University of Hong KongHong Kong SARChina Chinese University of Hong kong-Sichuan University Joint Laboratory in Reproductive Medicine The Chinese University of Hong KongHong Kong SARChina

Endometriosis is a common and benign angiogenesisdependent gynecological disorder,which refers to the proliferation and growth of endometrium-like tissues with neovasculature formation outside of the uterus.1 Available medical treatments for endometriosis containing hormonal and non-hormonal treatments had been limited for longterm usage by their side effects.2 Ideal medical treatment for endometriosis with efficacy to relieve symptoms and suppress endometriotic lesion growth and minimal side effects has been longing for decades.3 Angiogenesis is a promising therapeutic target for endometriosis.

关键词： therapeutic endometriosis herbal

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RBC aggregation,deformation and adhesion to endothelium:Role of nitric oxide derived from L-Arginine and sodium nitroprusside

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Journal of Innovative Optical Health Sciences 2024年第5期17卷 53-65页

作者： M.k.Maksimov p.B.Ermolinskiy O.N.Scheglovitova N.N.Sklyankina A.V.Muravyov A.E.Lugovtsov A.V.priezzhev Faculty of physics M.V.Lomonosov Moscow State University bld.1subbuilding.2Leninskie goryMoscow 11991Russia N.F.Gamaleya National Research Center for Epidemiology and Microbiology 18 Gamaleya StreetMoscow 123098Russia k.D.Ushinsky Yaroslavl State pedagogical University 108/1 Respublikanskaya StreetYaroslavl 150000Russia

Red blood cells(RBCs)are the most abundant human blood *** aggregation and deformation strongly determine blood viscosity which impacts hemorheology and *** turn,RBC properties depend on di®erent endogenous and exogenous *** such factor is nitric oxide(NO),which is mainly produced by endothelial cells(EC)from L-arginine amino acid in the circulatory *** the mechanisms of the RBC-endothelium interplay are not clear up to date and considering its possible clinical importance,the aims of this study are to investigate in vitro:(1)The e®ect of L-arginine induced NO on RBC aggregation and adhesion to endothelium;(2)the NO e®ect on RBC aggregation and deformation induced by L-arginine and sodium nitroprusside without the presence of endothelium in the *** RBC aggregation and adhesion to a monolayer of EC were studied using optical tweezers(OT).The RBC deformability and aggregation without endothelium in the samples were studied using the°ow chamber method and Myrenne *** con¯rmed that NO increases deformability and decreases aggregation of *** showed that the soluble guanylate cyclase pathway appears to be the only NO signaling pathway *** the samples with the endothelium,the\bell-shaped"dependence of RBC aggregation force on L-arginine concentration was observed,which improves our knowledge about the process of NO production by ***,data related to L-arginine accumulation by endothelium were obtained:Necessity of the presence of extracellular L-arginine stated by other authors was put under *** our study,NO decreased the RBC-endothelium adhesion,however,the tendency appeared to be weak and was not con¯rmed in another set of *** our knowledge,this is the¯rst attempt to measure the forces of RBC adhesion to endothelium monolayer with OT.

关键词： Red blood cells aggregation L-arginine endothelium optical tweezers Myrenne flow chamber

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*** model of riboflavin transporter deficiency(RTD)disorder reveals deficits in synaptic transmission and movement

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Genes & Diseases 2024年第4期11卷 120-123页

作者： Ramesh k.Narayanan Gonzalo perez-siles kamila A.Marzec Alexandra Boyling Brent Neumann Manoj p.Menezes Marina L.kennerson Northcott Neuroscience Laboratory ANZAC Research Institute-Sydney Local Health DistrictSydneyNSW 2139Australia Sydney Medical School University of SydneySydneyNSw 2050Australia ANZAC Research Institute-Sydney Local Health District SydneyNSW 2139Australia Neuroscience program Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental BiologyMonash UniversityMelbourneVictoria 3800Australia TY Nelson Department of Neurology and Neurosurgery and kids Neuroscience Children's Hospital at WestmeadWestmeadSydneyNSW2145Australia paediatrics and Child Health The University of SydneySydneySydneyNSW 2145Australia Molecular Medicine Laboratory Concord General Repatriation HospitalSydneyNSW 2139Australia

Riboflavin transporter deficiency(RTD),previously known as Brown-Vialetto-Van Laere syndrome,is a childhoodonset neurodegenerative disorder characterized by sensory and motor neuron degeneration causing ataxia,muscle weakness,optic atrophy,and respiratory *** in SLC52A2 and SLC52A3,solute carrier family members that encode riboflavin(RF)transporters RFVT2 and RFVT3,are known to cause RTD types 2 and 3,respectively.

关键词： RTD degeneration TD

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Verification and Validation of High-Resolution Inviscid and Viscous Conical Nozzle Flows

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Communications on Applied Mathematics and Computation 2024年第1期6卷 533-549页

作者： Luciano k.Araki Rafael B.de R.Borges Nicholas Dicati p.da Silva Chi-Wang Shu Department of Mechanical Engineering Federal University of ParanáCuritiba81531-980ParanáBrazil Mathematics and Statistics Institute Rio de Janeiro State UniversityRio de Janeiro20550-900Rio de JaneiroBrazil Department of Mechanical Engineering State University of MaringáMaringá87020-900ParanáBrazil Division of Applied Mathematics Brown UniversityProvidenceRI02912USA

Capturing elaborated flow structures and phenomena is required for well-solved numerical *** finite difference methods allow simple discretization of mesh and model ***,they need simpler meshes,e.g.,*** inverse Lax-Wendroff(ILW)procedure can handle complex geometries for rectangular ***-resolution and high-order methods can capture elaborated flow structures and *** also have strong mathematical and physical backgrounds,such as positivity-preserving,jump conditions,and wave propagation *** perceive an effort toward direct numerical simulation,for instance,regarding weighted essentially non-oscillatory(WENO)***,we propose to solve a challenging engineering application without turbulence *** aim to verify and validate recent high-resolution and high-order *** check the solver accuracy,we solved vortex and Couette ***,we solved inviscid and viscous nozzle flows for a conical *** employed the finite difference method,positivity-preserving Lax-Friedrichs splitting,high-resolution viscous terms discretization,fifth-order multi-resolution WENO,ILW,and third-order strong stability preserving *** showed the solver is high-order and captured elaborated flow structures and *** can see oblique shocks in both nozzle *** the viscous flow,we also captured a free-shock separation,recirculation,entrainment region,Mach disk,and the diamond-shaped pattern of nozzle flows.

关键词： High-resolution Compressible Navier-Stokes Free-shock separation Nozzle flow

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In vivo and in vitro study of resorbable magnesium wires for medical implants:Mg purity,surface quality,Zn alloying and polymer coating

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Journal of Magnesium and Alloys 2024年第6期12卷 2472-2488页

作者： k.Tesar J.Luňácková M.Jex M.Žaloudková R.Vrbová M.Bartoš p.klein L.Vištejnová J.Dušková E.Filová Z.Sucharda M.Steinerová S.Habr k.Balík A.Singh Department of Materials Faculty of Nuclear Sciences and Physical EngineeringCzech Technical University in PragueTrojanova 13Prague 12000Czech Republic Institute of physics Czech Academy of SciencesNa Slovance 2Prague 18221Czech Republic Institute of Dental Medicine First Faculty of MedicineCharles University and General University Hospital in PragueKaterinská32Prague 12801Czech Republic Department of physics Faculty of Nuclear Sciences and Physical EngineeringCzech Technical University in PragueBrehová7Prague 11519Czech Republic Department of Composites and Carbon Materials Institute of Rock Structure and MechanicsCzech Academy of SciencesV Holešovickách 41Prague 18209Czech Republic Institute of Anatomy First Faculty of MedicineCharles UniversityU Nemocnice 3Prague 12800Czech Republic Biomedical Center Faculty of Medicine in PilsenCharles UniversityAlej Svobody 76Pilsen 32300Czech Republic Department of pathological physiology Faculty of Medicine in PilsenCharles UniversityAlej Svobody 7632300 PilsenCzech Republic Department of Histology and Embryology Faculty of Medicine in PilsenCharles UniversityAlej Svobody 7632300 PilsenCzech Republic Institute of pathology First Faculty of MedicineCharles University and General University Hospital in PragueStudnickova 2Prague 12800Czech Republic Department of Biomaterials and Tissue Engineering Institute of PhysiologyCzech Academy of SciencesVidenska 1083Prague 14200Czech Republic Research Center for Structural Materials National Institute for Materials Science1-2-1 SengenTsukuba 305-0047Japan

Magnesium is an excellent material in terms of biocompatibility and its corrosion products can serve as an active source for new bone ***,localized corrosion and H_(2)generation limit the potential of Mg-based *** low-alloyed Mg-Zn wires can strongly reduce problems with large H_(2)bubbles and improve the mechanical properties considerably while maintaining excellent long-term *** pickling and a polymer coating can be effectively used to lower the rate of in vivo *** this work,microstructural,mechanical,and in vitro characterization of 250μm and 300μm extruded wires made from ultra-pure Mg,commercially pure Mg,Mg-0.15Zn,Mg-0.4Zn and Mg-1Zn was ***,Mg-0.4Zn wires together with a variant coated with a copolymer of L-lactide andε-caprolactone were tested in vivo on artificially damaged Wistar rat *** on the observed Mg-induced osteogenesis,polymer-coated Mg wires with a small addition of Zn are a perspective material for bone-support applications,such as cerclage and fixation wires.

关键词： Magnesium Resorbable Mg wire Mg-Zn implant degradation Biocompatibility study Zn grain boundary segregation

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AcWRkY28 mediated activation of AcCpk genes confers salt tolerance in pineapple(Ananas comosus)

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Horticultural plant Journal 2024年第2期10卷 398-412页

作者： Qiao Zhou Samaranayaka Vidana Gamage Nirosha priyadarshani Rongjuan Qin Han Cheng Tiantian Luo Myat Hnin wai Mohammad Aqa Mohammadi Yanhui Liu Chang Liu Hanyang Cai Xiaomei Wang Yeqiang Liu Yuan Qin Lulu Wang Guangxi key Lab of Sugarcane Biology State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureGuangxi UniversityNanningGuangxi 530004China State key Laboratory of Ecological pest Control for Fujian and Taiwan Crops College of Life ScienceCollege of Plant ProtectionCollege of AgricultureCollege of HorticultureFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyCenter for Genomics and BiotechnologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China pingtan Science and Technology Research Institute of Fujian Agriculture and Forestry University PingtanFujian 350400China National Education Commission BMICHColombo00700Sri Lanka Fishery Multiplication Management Station of Lijiang River Water Supply Hub project GuilinGuangxi 541001China Horticulture Research Institute Guangxi Academy of Agricultural SciencesNanning Investigation Station of South Subtropical Fruit TreesMinistry of AgricultureNanningGuangxi 530007China

Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRkY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRkY genes is poorly understood. Here, we studied the pineapple WRkY gene, AcWRkY28, by generating AcWRkY28 over-expressing transgenic pineapple plants. Overexpression of AcWRkY28 enhanced the salt stress resistance in transgenic pineapple lines. Comparative transcriptome analysis of transgenic and wild-type pineapple plants showed that “plant-pathogen interaction” pathway genes, including 9calcium-dependent protein kinases (Cpks), were up-regulated in AcWRkY28 over-expressing plants. Furthermore, chromatin immunoprecipitation and yeast one-hybrid assays revealed AcCpk12, AcCpk3, AcCpk8, AcCpk1, and AcCpk15 as direct targets of AcWRkY28. Consistently, the study of AcCpk12 over-expressing Arabidopsis lines showed that AcCpk12 enhances salt, drought, and disease resistance. This study shows that AcWRkY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCpk genes.

关键词： pineapple AcWRkY AcCpk Transcription factor Salt stress ChIp

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Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期12卷 549-557页

作者： Yan Zheng Ei Ei khine khin Mar Thi Ei Ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay wai Lin khin Than Oo Myat Myat Moe San San Aye Weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian provincial key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation

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NHE7 upregulation potentiates the uptake of small extracellular vesicles by enhancing maturation of macropinosomes in hepatocellular carcinoma

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Cancer Communications 2024年第2期44卷 251-272页

作者： Yue Yao Yi Xu Liang Yu Ting-Mao Xue Zhi-Jie Xiao pui-Chi Tin Hiu-Ling Fung Hoi-Tang Ma Jing-ping Yun Judy wai ping Yam Department of pathology School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongP.R.China Department of Endocrinology and Metabolism Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjingP.R.China Department of Hepatopancreatobiliary Surgery Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjingP.R.China State key Laboratory of Oncology in South China Cancer Center of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Department of Hepatobiliary Surgery II Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China Scientific Research Center The Seventh Affiliated HospitalSun Yat-sen UniversityShenzhenGuangdongP.R.China State key Laboratory of Liver Research The University of Hong KongHong KongP.R.China Department of pathology Cancer Center of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China

Background:Small extracellular vesicles(sEVs)mediate intercellular commu-nication that contributes to hepatocellular carcinoma(HCC)progression via multifaceted *** success of cell entry determines the effect of sEV on recipient ***,we aimed to delineate the mechanisms underlying the uptake of sEV in ***:AF,Alexa Fluor;ANOVA,analysis of variance;ATp9A,ATpase phospholipid Transporting 9A;BCECF-AM,2’,7’-bis-(2-barboxyethyl)-5-(and-6)-carboxyfluorescein,acetoxymethyl ester;BSA,bovine serum albumin;CCMR,Centre for Comparative Medicine Research;CRISpR,clustered regularly interspaced short palindromic repeats;CTL,ctrl;CXCR4,C-X-C Chemokine Receptor Type 4;DApI,4′,6-diamidino-2-phenylindole;DFS,disease-free survival;DMEM,Dulbecco’s Modified Eagle Medium;DMSO,dimethyl sulfoxide;Dox,doxycycline;EEA1,early endosome antigen 1;EIpA,5-(N-ethyl-N-isopropyl)-amiloride;FBS,fetal bovine serum;FITC,fluorescein isothiocyanate;GApDH,glyceraldehyde-3-phosphate dehydrogenase;GM130,Golgi matrix protein 130;HCC,hepatocellular carcinoma;HEpES,4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;HpRT1,hypoxanthine phosphoribosyltransferase 1;H-score,histoscore;IAA,indole-3-acetic acid;kD,knockdown;kO,knockout;mAID,mini-auxin-inducible degron;MTT,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;NHE7,Na(+)/H(+)exchanger 7;ns,non-significant;OD,optical density;OS,overall survival;pBS,phosphate-buffered saline;pCR,polymerase chain reaction;pHe,endosomal pH;pHi,intracellular pH;pkH67,paul karl Horan 67;Rab21,Ras-associated binding protein 21;RIpA,radioimmunoprecipitation assay;SAM,synergistic activation mediator;SEMs,standard error of the means;sEVs,small extracellular vesicles;sgRNA,single-guide RNA;shRNA,short-hairpin RNA;SLC9,solute carrier gene 9;SLiCE,Seamless Ligation Cloning Extract;TCGA,The Cancer Genome Atlas;TCL,total cell lysates;TGN,trans-Golgi network;TMA,tissue microarray;TMR,tetramethyl rhodamine;TSG101,tumor susceptibility gene *** Yao an

关键词： hepatocellular carcinoma small extracellular vesicles macropinocytosis pH regulation sodium-hydrogen exchanger

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