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Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions

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Neural Regeneration Research 2011年第34期6卷 2681-2686页

作者： Xiaodong Yuan Yanan Cai Ya ou Yanhui lu Department of Neurology Kailuan General Hospital Hebei Union University Tangshan 063000 Hebei Province China

β-mercaptoethanol was used to induce in vitro neuronal differentiation of adipose-derived stromal cells. Within an 8-hour period post-differentiation, the induced cells exhibited typical neuronal morphology, and expression of microtubule-associated protein 2 and neuron-specific enolase, which are markers of mature neurons, reached a peak at 5 hours. Specific organelle Nissl bodies of neurons were observed under transmission electron microscopy. Results of membrane potential showed that fluorescence intensity of cells was greater after 5 hours than adipose-derived stromal cells prior to induction. In addition, following stimulation with high-concentration potassium solution, fluorescence intensity increased. These experimental findings suggested that neurons differentiated from adipose-derived stromal cells and expressed mature K^＋ channels. In addition, following stimulation with high potassium solution, the membrane potential depolarized and fired an action potential, confirming that the induced cells possessed electrophysiological functions.

关键词： adipose-derived stromal cells differentiation membrane potential neurons ultrastructure electrophysiological functions DiBAC4 (3)

Apoptosis during β-mercaptoethanol-induced differentiation of adult adipose-derived stromal cells into neurons

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Neural Regeneration Research 2011年第10期6卷 750-755页

作者： Yanan Cai Xiaodong Yuan Ya ou Yanhui lu Department of Neurology Kailuan General Hospital Hebei United University Tangshan 063000 Hebei Province China

β-mercaptoethanol can induce adipose-derived stromal cells to rapidly and efficiently differentiate into neurons in ***,because of the short survival time of the differentiated cells,clinical applications for this technique are *** such,we examined apoptosis of neurons differentiated from adipose-derived stromal cells induced with β-mercaptoethanol in vitro using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and transmission electron *** results revealed that the number of surviving cells decreased and apoptosis rate increased as induction time *** together,these results suggest that apoptosis occurring in the process of adipose-derived stromal cells differentiating into neurons is the main cause of cell ***,the mechanism underlying cellular apoptosis should be researched further to develop methods of controlling apoptosis for clinical applications.

关键词： adult adipose-derived stromal cells induced differentiation neurons ultrastructure apoptosis 13-mercaptoethanol neural regeneration

养老机构老年人自立援助认知现状及影响因素分析

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中国实用护理杂志 2024年第17期40卷 1325-1331页

作者：欧璐陈圆唐艳何丽平邹黄珏梁高科王卫红湖南师范大学医学院护理系长沙410006 长沙卫生职业学院护理学院长沙410605

目的了解养老机构老年人自立援助认知现状及影响因素,为构建养老机构自立援助干预体系,更有效地实施自立援助提供参考依据。方法本研究为横断面研究,2023年4—8月采用便利抽样法选取长沙市7所养老机构的320名老年人为研究对象,采用一般... 详细信息

目的了解养老机构老年人自立援助认知现状及影响因素,为构建养老机构自立援助干预体系,更有效地实施自立援助提供参考依据。方法本研究为横断面研究,2023年4—8月采用便利抽样法选取长沙市7所养老机构的320名老年人为研究对象,采用一般资料调查表、Barthel指数评定量表、领悟社会支持量表及自行编制的养老机构老年人自立援助知信行问卷对其进行调查。采用多元线性回归分析养老机构老年人自立援助的影响因素。结果回收有效问卷315份,其中男95名,女220名,以80~89岁居多,为159名;养老机构老年人自立援助知信行问卷得分为(91.24±11.92)分,条目均分为(3.51±0.46)分。多元线性回归分析显示,活动频率、日常生活活动能力及领悟社会支持为影响自立援助的主要因素(t=5.02、-6.67、9.48,均P<0.05),可联合解释51.8%的变异。结论养老机构老年人的自立援助处于偏高水平,相关人员可通过对老年人进行自立援助健康教育、定期组织活动及加强社会支持建设等方式,以更好地发挥养老机构老年人的主观能动性。

关键词：老年人养老机构自立援助认知影响因素

Triple Nine Paves the Way for State-Owned Enterprises

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China Today 2000年第1期49卷 30-34页

作者： ou lu

Provides information on Triple Nine Group which has grown from a single product-based company into a large industrial group in the country. Revolutionary marketing strategies of Triple Nine; Management mechanism of Tr... 详细信息

关键词： Triple Nine Paves the Way for State-Owned Enterprises In

Experimental study of rotating wind turbine breakdown characteristics in large scale air gaps

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Plasma Science and Technology 2017年第6期19卷 108-115页

作者： Yu WANG lu ou Tianjun SI Yang NI Jianwei XU Xishan WEN School of Electrical Engineering Wuhan University Wuhan 430072 Hubei Province People＇s Republic of China

When a wind turbine is struck by lightning,its blades are usually *** effect of blade rotation on a turbine＇s ability to trigger a lightning strike is ***,an arching electrode was used in a wind turbine lightning discharge test to investigate the difference in lightning triggering ability when blades are rotating and stationary.A negative polarity switching waveform of 250/2500 μs was applied to the arching electrode and the up-and-down method was used to calculate the 50%discharge *** discharge tests of a 1：30 scale wind turbine model with 2,4,and 6 m air gaps were performed and the discharge process was *** experimental results demonstrated that when a 2 m air gap was used,the breakdown voltage increased as the blade speed was increased,but when the gap length was 4 m or longer,the trend was reversed and the breakdown voltage *** analysis revealed that the rotation of the blades changes the charge distribution in the blade-tip region,promotes upward leader development on the blade tip,and decreases the breakdown ***,the blade rotation of a wind turbine increases its ability to trigger lightning strikes.

关键词： wind turbine rotation up-and-down method breakdown characteristic large-scale air gap

William Man: A Man of Integrity

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China Today 2000年第4期49卷 34-36页

作者： ou lu

Features William Man, a returned overseas Chinese. How he started as an entrepreneur in Great Britain; Details on his return to Hong Kong, China; Details on the residence rights of overseas Chinese and their descendan... 详细信息

关键词： William Man A Man of Integrity

Celebrating Macao's Return to China

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China Today 2000年第2期49卷 9-13页

作者： ou lu

ChinaresumeditsexerciseofsovereigntyoverMacaoonDecember20,******... 详细信息

ChinaresumeditsexerciseofsovereigntyoverMacaoonDecember20,******...

关键词： In Celebrating Macao’s Return to China

Strategies for flame-retardant polymer electrolytes for safe lithiumbased batteries

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Nano Research 2024年第10期17卷 8754-8771页

作者： Xiao Ma Yang lu Yu ou Shuaishuai Yan Wenhui Hou Pan Zhou Kai Liu Department of Chemical Engineering Tsinghua UniversityBeijing 100084China

The advancement of lithium-based batteries has spurred anticipation for enhanced energy density,extended cycle life and reduced capacity ***,these benefits are accompanied by potential risks,such as thermal runaway and explosions due to higher energy ***,liquid organic electrolytes are the predominant choice for lithium batteries,despite their limitations in terms of mechanical strength and vulnerability to *** development of polymer electrolytes,with their high Young’s modulus and enhanced safety features,offers a potential solution to the drawbacks of traditional liquid *** these advantages,polymer electrolytes are still susceptible to burning and *** address this issue,researchers have conducted extensive studies to improve their flame-retardant properties from various *** review provides a concise overview of the thermal runaway mechanisms,flame-retardant mechanisms and electrochemical performance of polymer *** also outlines the advancements in flame-retardant polymer electrolytes through the incorporation of various additives and the selection of inherently flame-retardant *** review aims to offer a comprehensive understanding of flame-retardant polymer electrolytes and serve as a guide for future research in this field.

关键词： lithium batteries polymer electrolyte thermal runaway flame-retardant additives energy storage materials

基于监督拉普拉斯分值和主元分析的滚动轴承故障诊断

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机械工程学报 2014年第5期50卷 88-94页

作者：欧璐于德介湖南大学汽车车身先进设计制造国家重点实验室长沙410082

在拉普拉斯分值(Laplaian score,LS)方法的基础上,提出一种监督拉普拉斯分值(Supervised laplaian score,SLS)特征选择方法。该方法同时考虑数据的标号信息和局部几何结构,避免LS方法中要设定近邻图参数的问题。将SLS和主元分析(Princip... 详细信息

在拉普拉斯分值(Laplaian score,LS)方法的基础上,提出一种监督拉普拉斯分值(Supervised laplaian score,SLS)特征选择方法。该方法同时考虑数据的标号信息和局部几何结构,避免LS方法中要设定近邻图参数的问题。将SLS和主元分析(Principal component analysis,PCA)相结合,提出基于SLS和PCA的滚动轴承故障诊断方法。该方法在时域和频域对滚动轴承振动信号进行特征提取,组成初始特征矢量;利用SLS进行特征选择,形成故障特征矢量;再对特征矩阵进行PCA降维处理,并用K近邻(K-nearest neighbor algorithm,KNN)分类算法实现滚动轴承不同故障类型的识别。应用实例表明,该方法能有效提取滚动轴承振动信号特征,诊断滚动轴承故障,且故障分辨率优于基于LS和PCA的故障诊断方法。

关键词：特征选择监督拉普拉斯分值主元分析故障诊断