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The Effects of Several Types of Induced Abiotic Stress on <i>Cephalaria joppensis</i>Germination under Controlled Conditions

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American Journal of Plant Sciences 2018年第7期9卷 1486-1499页

作者： Miriam Daklo-Keren Waffa Abu-Aklin orit Cohen Valantina Berger Galina Sidan Aharon Bellalou orit amir-segev Ran Hovav Shmuel Galili Seed Laboratory Institute of Plant Sciences Agricultural Research Organization The Volcani Center Rishon Le Ziyyon Israel Vegetables & Field Crops Science Department Institute of Plant Sciences Agricultural Research Organization The Volcani Center Rishon Le Ziyyon Israel

The recently domesticated species, Cephalaria joppensis (CJ), is emerging as a new alternative forage crop in Israel. It has high biomass potential and nutritional values that are comparable to forage wheat. However, CJ emerges slowly under cold conditions, which hinders its development as a major winter crop. Additional tolerance for abiotic stress would improve its performance as a forage crop. We examined the effects of several abiotic factors (i.e., cold, salinity, drought and pH) on CJ germination under controlled conditions. The effect of temperature was studied by incubating seeds at different temperatures between 7°C and 35°C. The effects of salinity, osmotic potential and pH were tested by incubating seeds at different NaCl, PEG and pH levels, respectively. Temperature, salinity and osmotic potential significantly affected germination;whereas pH did not. Temperature did not affect the final proportion of germinated seeds, but did affect other germination-rate variables, indicating that germination rate might be the limiting factor under field conditions. Salinity also affected germination-rate variables, but not the proportion of seeds that germinated. Notably, CJ was found to be relatively resistant to high salt concentrations, with a 273 mM NaCl threshold for germination, indicating its potential as a relatively salt-tolerant forage crop. Both the proportion of germinated seeds and the germination rate were highly sensitive to the osmotic-potential treatments, indicating that drought resistance will remain the biggest challenge for CJ. This study provides baseline data for a rapid and efficient system for further screening for abiotic-stress tolerance among wild and cultivated lines of CJ.

关键词： Cephalaria joppensis Abiotic Stress Seed Germination

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Metal-dependent anaerobic methane oxidation in marine sediment:insights from marine settings and other systems

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Science China(Life Sciences) 2019年第10期62卷 1287-1295页

作者： Lewen Liang Yinzhao Wang orit Sivan Fengping Wang State Key Laboratory of Microbial Metabolism School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240 China Joint International Research Laboratory of Metabolic & Developmental Sciences Shanghai Jiao Tong UniversityShanghai 200240China Department of Geological and Environmental Sciences Ben-Gurion University of the NegevBeer-Sheva 84105Israel

Anaerobic oxidation of methane(AOM) plays a crucial role in controlling global methane emission. This is a microbial process that relies on the reduction of external electron acceptors such as sulfate, nitrate/nitrite, and transient metal ions. In marine settings, the dominant electron acceptor for AOM is sulfate, while other known electron acceptors are transient metal ions such as iron and manganese oxides. Despite the AOM process coupled with sulfate reduction being relatively well characterized,researches on metal-dependent AOM process are few, and no microorganism has to date been identified as being responsible for this reaction in natural marine environments. In this review, geochemical evidences of metal-dependent AOM from sediment cores in various marine environments are summarized. Studies have showed that iron and manganese are reduced in accordance with methane oxidation in seeps or diffusive profiles below the methanogenesis zone. The potential biochemical basis and mechanisms for metal-dependent AOM processes are here presented and discussed. Future research will shed light on the microbes involved in this process and also on the molecular basis of the electron transfer between these microbes and metals in natural marine environments.

关键词： anaerobic methane oxidation metal-AOM marine sediment archaea electron transfer

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A new algorithm for the diagnosis of celiac disease

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Cellular & Molecular Immunology 2011年第2期8卷 146-149页

作者： orit Rozenberg Aaron Lerner Avi Pacht Maya Grinberg Dina Reginashvili Clara Henig Mira Barak Central Laboratory of Haifa and Western Galilee Clalit Health Services and Pediatric GastroenterologyHaifaIsrael Nutrition Unit Carmel Medical CenterHaifaIsrael Technion-Israel Institute of Technology HaifaIsrael

Celiac disease(CD)affects at least 1%of the Western population but remains largely *** our laboratory,we utilize a novel algorithm to diagnose pediatric CD that offers both high sensitivity and high specificity for diagnosis in an outpatient *** aim of the present study was to challenge this algorithm and to test its performance in children and adults suspected of having *** a three-assay algorithm,screening with the most sensitive tissue transglutaminase(tTG)complexed with deamidated gliadin peptide neoepitope immunoglobulin A(IgA)1IgG assay and confirming with the two specific tTG IgA and tTG IgA1IgG assays,we examined the serological results from 112 children aged 0–17 years old and 60 adults in comparison to their respective biopsy *** algorithm performance was calculated by statistical *** use of the new algorithm enabled us to diagnose CD with 98%sensitivity,93%specificity and 95%accuracy in the pediatric group and 94%sensitivity,92%specificity and 93%accuracy in the total population *** false-negative cases in the adult group were attributed to previous adherence to a gluten-free diet,and the single false-negative result in a young child became a true positive after 6 *** have also monitored three celiac patients before and after diagnosis and found that the algorithm may be suitable for disease *** newly proposed three-assay algorithm for celiac detection is very reliable in both children and *** to the high performance of this assay,the further need for confirmatory intestinal biopsies will be reassessed.

关键词： celiac disease diagnosis intestinal histopathology sensitivity specificity tissue transglutaminase

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Diet of patients after pouch surgery may affect pouch inflammation

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World Journal of Gastroenterology 2013年第38期19卷 6458-6464页

作者： orit Ianco Hagit Tulchinsky Michal Lusthaus Amos Ofer Erwin Santo Nachum Vaisman Iris Dotan Comprehensive Pouch Clinic IBD CenterDepartment of Gastroenterology and Liver DiseasesTel Aviv Sourasky Medical CenterTel Aviv 64239Israel Sackler Faculty of Medicine Tel Aviv UniversityTel Aviv 64239Israel Comprehensive Pouch Clinic and the Proctology Unit Department of SurgeryTel Aviv Sourasky Medical CenterTel Aviv 64239Israel Unit of Clinical Nutrition Tel Aviv Sourasky Medical CenterTel Aviv 64239Israel

AIM:To investigate the diet of pouch patients compared to healthy controls,and to correlate pouch patients’diet with disease ***:Pouch patients were recruited and prospectively followed-up at the Comprehensive Pouch Clinic at the Tel Aviv Sourasky Medical *** behavior was determined based on clinical,endoscopic and histological *** age-and sex-matched volunteers were selected from the"MABAT"Israeli Nutrition and Public Health Governmental Study and served as the control *** the participants completed a 106-item food frequency questionnaire categorized into food groups and nutritional values based on those used in the United States Department of Agriculture food pyramid and the Israeli food *** on Dietary behavior,food avoidance,the use of nutritional supplements,physical activity,smoking habits,and body-mass index(BMI)were also *** patients who had familial adenomatous polyposis(n=3),irritable pouch syndrome(n=4),or patients whose pouch surgery took place less than one year previously(n=5)were excluded from ***:The pouch patients(n=80)consumed significantly more from the bakery products food group(1.2±1.4 servings/d vs 0.6±1.1 servings/d,P<0.05)and as twice as many servings from the oils and fats(4.8±3.4 servings/d vs 2.4±2 servings/d,P<0.05),and the nuts and seeds food group(0.3±0.6 servings/d vs 0.1±0.4 servings/d,P<0.05)compared to the controls(n=80).The pouch patients consumed significantly more total fat(97.6±40.5 g/d vs 84.4±39 g/d,P<0.05)and fat components[monounsaturated fatty acids(38.4±16.4 g/d vs 30±14 g/d,P<0.001),and saturated fatty acids(30±15.5 g/d vs 28±14.1 g/d,P<0.00)]than the *** contrast,the pouch patients consumed significantly fewer carbohydrates(305.5±141.4 g/d vs 369±215.2 g/d,P=0.03),sugars(124±76.2 g/d vs 157.5±90.4 g/d,P=0.01),theobromine(77.8±100 mg/d vs 236.6±244.5 mg/d,P<0.00),retinol(474.4±337.1μg/d vs 832.4±609.6μg/d,P<0.001)and dietary fibers(26.2±15.4

关键词： Ulcerative colitis Dietary reference intake Body mass index Ileal-pouch anal anastomosis Pouch surgery Food frequency questionnaire

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Late onset fulminant Wilson's disease:A case report and review of the literature

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World Journal of Gastroenterology 2014年第46期20卷 17656-17660页

作者： Ella Weitzman orit Pappo Peretz Weiss Moshe Frydman Yael Haviv-Yadid Ziv Ben Ari Liver Disease Center Sheba Medical CenterRamat Gan 52621Israel Department of Pathology Sheba Medical CenterRamat Gan 52621Israel Institute of Genetics Sheba Medical CenterRamat Gan 52621Israel Intensive Care Unit Sheba Medical CenterRamat GanTel Aviv UniversityRamat Gan 52621Israel Sackler School of Medicine Tel Aviv UniversityTel Aviv 69975Israel

Wilson’s disease(WD)is an autosomal recessive inherited disorder of hepatic copper *** can be present in different clinical conditions,with the most common ones being liver disease and neuropsychiatric *** cases present symptoms at<40years of ***,few reports exist in the literature on patients in whom the disease presented beyond this *** this report,we present a case of late onset fulminant WD in a 58-year-old patient in whom the diagnosis was established clinically,by genetic analysis of the ATP7B gene disclosing rare mutations(G1099S and c.1707+3ins T)as well as by high hepatic copper *** also reviewed the relevant *** diagnosis of WD with late onset presentation is easily *** diagnostic features and the geneticbackground in patients with late onset WD are not different from those in patients with early onset WD,except for the *** treatments for this disorder that can be fatal are available and will prevent or reverse many manifestations if the disease is discovered early.

关键词： Wilson's disease Late onset Fulminant ATP7B gene mutations Copper

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＂It Is Not Our Business＂： Perceptions of Department Heads of Teacher Education Programs Regarding Inclusion

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Psychology Research 2015年第11期5卷 645-654页

作者： orit Gilor Pnina Shavit Beit Berl College Israel

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Salvage logging versus natural regeneration post-fire practices in a forest: Soil chemical and microbial aspects

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Open Journal of Ecology 2012年第1期2卷 29-37页

作者： orit Ginzburg Yosef Steinberger The Mina & Everard Goodman Faculty of Life Sciences Bar-Ilan University Ramat-Gan Israel

The increase in forest-wildfire events around the world has revived the old debate regarding the practice of salvage logging and its effectiveness in comparison to the unlogged, natural regeneration of burned forests. Since the logging of burned trees may have undesirable outcomes in soil systems, such as soil compaction and nutrient losses, these changes could further cause disturbances to soil microbial-community activity. The aim of this study was to examine chemical and biological changes in soil under two post-fire practices: salvage logging and unlogged, natural regeneration of burned areas, in the recently burned Byria Forest in Israel. Results indicated that salvage logging had a short- lived effect on soil chemical and biological properties that was confined mostly to the first year after logging. Soil moisture was greatly affected by salvage logging, and drier conditions were found in the logged compared to the unlogged burned areas. Moreover, logging had a negative effect on microbial biomass, with reduced biomass in the logged compared to unlogged areas, which was more evident during the first year after fire. These findings support the recommendations in the literature to postpone post-fire practices such as logging or, in turn, to combine the two practices in order to create a mosaic of burned-logged and burned-naturally regenerated areas.

关键词： Microbial community Natural regeneration Post-fire practice Salvage logging Wildfire

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Co-occurrence of type 1 diabetes mellitus and celiac disease

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World Journal of Diabetes 2015年第5期6卷 707-714页

作者： Amit Akirov orit Pinhas-Hamiel Beilinson Campus Rabin Medical Center Sackler School of Medicine Tel-Aviv University Pediatric Endocrine and Diabetes Unit Safra Children’s HospitalSheba Medical Center

The co-occurrence of celiac disease(CD) and type 1 diabetes(T1DM) has been reported as 5-7 times more prevalent than CD *** clinical presentation and natural history of CD in patients with T1 DM may vary *** than 10% of patients with T1 DM and CD show gastrointestinal ***,experts support screening for CD in T1 DM patients,though there is no consensus as to the recommended frequency of *** stratified by time since CD diagnosis,longer follow-up and coexistence of CD are associated with significant increased risk of diabetic associated morbidity and *** CD diagnosis and treatment with a gluten-free diet are essential.

关键词： Type 1 diabetes mellitus Celiac disease Glycemic control Gluten free diet Pediatrics

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Impact of glucose level on morbidity and mortality in elderly with diabetes and pre-diabetes

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World Journal of Diabetes 2015年第2期6卷 345-351页

作者： orit Twito Meir Frankel Dan Nabriski Endocrinology Diabetes and Metabolism InstituteMeir Medical Center

The prevalence of type 2 diabetes mellitus(DM) increases with age and reaches 25% in those older than age 65 years. Pre-diabetes status is also very common in the elderly, and is present in about half of those age 75 years and older. Many physicians care for elderly patients with diabetes and pre-diabetes, dealing with the challenge of controlling glucose levels and improving health with minimal adverse events. Over the last decade, research on diabetes among the elderly population has proliferated, adding new information on this topic. This review summarizes the updated medical literature on diabetes and pre-diabetes in the elderly, including the significance of pre-diabetic conditions, new-onset DM in the elderly and long-standing DM. The role of therapeutic intervention and the level of glycemic control for this population are discussed in particular.

关键词： Diabetes mellitus Elderly Old age Prediabetes Glycemic control

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Effect of hepatic iron concentration reduction on hepatic fibrosis and damage in rats with cholestatic liver disease

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World Journal of Gastroenterology 2006年第2期12卷 240-245页

作者： Gil Peretz Gabriela Link orit Pappo Rafael Bruck Zvi Ackerman Department of Internal Medicine Hebrew University- Hadassah Medical CenterMount Scopus CampusJerusalemIsrael Department of Human Nutrition and Metabolism Hebrew University Hadassah Medical SchoolJerusalemIsrael Department of Pathology Hebrew University-Hadassah Medical CenterJerusalemIsrael Department of Gastroenterology and Hepatology The EWolfson Medical CenterHolonand Sackler School of MedicineTel Aviv UniversityTel AvivIsrael

AIM： TO assess the effect of iron reduction after phlebotomy in rats with ＂normal＂ hepatic iron concentration （HIC） on the progression of hepatic fibrosis, as a result of bile duct ligation （BDL）. METHODS： Rats underwent phlebotomy before or after sham operation or BDL. Animals undergone only BDL or sham operation served as controls. Two weeks after surgery, indices of hepatic damage and fibrosis were evaluated. RESULTS： Phlebotomy lowered HIC. Phlebotomy after BDL was associated with body weight increase, lower hepatic weight, less portal hypertension, less periportal necrosis, less portal inflammation, lower hepatic activity index score and higher albumin levels. On the other hand, phlebotomy before BDL was associated with body weight decrease and hepatic activity index score increase. Phlebotomy after sham operation was not associated with any hepatic or systemic adverse effects. CONCLUSION： Reduction of HIC after induction of liver damage may have beneficial effects in BDL rats. However, iron deficiency could induce impairment of liver function and may make the liver more susceptible to insults like BDL.

关键词： Iron Phlebotomy Bile duct ligation Hepatic activity index Rat.

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