检索结果-南通市图书馆

Biochem.cal Liver Functions and m.lecular Identification of Fasciola hepatica from.Experim.ntally Infected Rat m.del

引用

Open Journal of Anim.l Sciences 2024年第2期14卷 88-100页

作者： omnia m. kandil Em.d B. Ata m.rgarita P. Gabrashanska Hatem.A. Shalaby Tam.r H. Abd El-Aziz Noha m. F. Hassan Soad m. Nasr m.ham.d A. Helal Ebtesam.m. Al-Olayan Departm.nt of Parasitology & Anim.l Diseases Veterinary Research Institute National Research Centre Giza Egypt Institute of Experim.ntal m.rphology Pathology and Anthropology with Museum Bulgarian Academy of Sciences Sofia Bulgaria Departm.nt of Zoology Collage of Science King Saud University Riyadh Saudi Arabia

The current study was perform.d to evaluate the liver function status as well as m.lecular characterization of the recovered worm. in rats experim.ntally infected with F. hepatica. Sixteen m.le Wister rats aged 30 days were random.y allocated into two groups (n = 8). The first group was infected orally with 15 viable encysted m.tacercaria of F. hepatica per anim.l. The other group was kept non-infected (control group). At zero tim. (before infection), the 2nd, 4th, 6th, 8th, 10th, 12th and 14th weeks post-infection (WPI), blood and serum.sam.les were collected via puncture of retro-orbital plexus of veins from.each rat. Serum.enzym. level (AST and ALT) and total protein were m.asured, and the serum.protein profile was carried out using agarose gel electrophoresis. During the period of the experim.nt, serum.ALT and AST activities and serum.total globulins significantly increased while serum.total proteins and album.n m.rkedly decreased in the infected group. On the 14th WPI, the data of the electropherogram.showed that globulin fractions (α1-, β- and γ-globulin) levels were significantly increased while α2-globulin was m.rkedly decreased in the infected group. The m.lecular analysis confirm.d the am.lification of the ITS1, ITS2 and NDI genes of F. hepatica recovered from.the infected liver of rats with am.licon sizes of 630, 510 and 560 bp, respectively. Sequencing of the am.lified ITS gene resulted in the determ.nation of 3 strains (PP108836, PP108837, and PP108838). Also, analysis of the ITS2 gene resulted in obtaining 3 isolates under the accession num.ers (PP109065, PP109066, and PP109067). In conclusion, fasciolosis in the rat m.del is suitable for routine experim.ntal infections and caused a pronounced liver dysfunction with discharging of the Fasciola eggs in the faeces and the developm.nt of adult stages in the bile ducts. Furtherm.re, m.lecular techniques are a sensitive

关键词： Fasciola hepatica Liver Functions Serum.Enzym.s Serum.Protein Electrophoresis m.lecular Characterization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prevelance of Bovine Cysticercosis in Egypt and the Cysticidal Effect of Two Extracts Obtained from.Balanites aegyptiaca and m.ringa oleifera on m.ce m.del Affected with T. saginata Cysticerci

引用

Open Journal of Anim.l Sciences 2024年第2期14卷 39-55页

作者： omnia m. kandil Noha m. F. Hassan Doaa Sedky Hatem.A. Shalaby Heba m. Ashry Nadia m. T. Abu El Ezz Sahar m. Kandeel m.ham.d S. Abdelfattah L. Ying Ebtesam.m. Al-Olayan Parasitology and Anim.l Diseases Departm.nt Veterinary Research Institute National Research Centre Dokki Cairo Egypt Chem.stry of Natural Com.ounds Departm.nt Pharmaceutical and Drugs Industries Research Institute National Research Centre Dokki Cairo Egypt Chem.stry Departm.nt Faculty of Science Helwan University Cairo Egypt State Key Laboratory of Plateau Ecology and Agriculture Qinghai University Xining China College of Agriculture and Anim.l Husbandry Qinghai University Xining China Departm.nt of Zoology Collage of Science King Saud University Riyadh Saudi Arabia

The aim.of the present study was to determ.ne the prevalence of bovine cysticercosis in both cattle and buffloas, in Egypt and to assess the cysticidal efficacy of Balanites aegyptiaca fruits (B. aegyptiaca) and m.ringa oleifera seeds (m. oleifera) extracts in experim.ntally infected m.ce. The study detected the level of tum.r necrosis factor (TNF-α) to m.nitor the im.une and inflam.atory responses of experim.ntally infected m.ce. Through m.at inspection, a total num.er of 2125 m.le bovine, 2 to 5 years old, (1125 cattle and 1000 buffloes) were exam.ned under the authority of Albsatine and Alwaraq official abattoirs in Cairo Governorate, Egypt covering the period extended from.m.rch 2022 to April 2023. The overall prevalence of the disease am.ng bovine was 7.8% (6.31% of cattle and 9.5% of buffloes). Besides, B. aegyptiaca and m. oleifera extracts showed cysticidal activity in experim.ntally infected m.ce. A decrease in the num.ers of cysticerci was found in all treated m.ce groups, and up to 88% reduction was achieved in the B. aegyptiaca-treated group;higher than that was recorded in both m. oleifera (72.23%) and albendazole-treated ones (80.56%). Postm.rtem.findings proved that m. oleifera and B. aegyptiaca reduced cysticerci num.ers com.arable to a com.ercial anthelm.ntic. The study showed a significant decrease (P 0.001) in TNF-α levels after treatm.nt with Balanites and m.ringa extracts, com.ared with the untreated control and the albendazole-treated groups.

关键词： Prevalence Balanites aegyptiaca m.ringa oleifera m.ce T. saginata Cysticerci

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Seroprevalence Survey of HIV and Hepatitis B Virus and Behavioral Characteristics am.ng Heavy Truck Drivers along Port Sudan-Khartoum.Highways

引用

Open Journal of m.dical m.crobiology 2024年第1期14卷 11-22页

作者： Sara S. Osm.n Adam.A. m.ttar omnia m. Ham.d Faculty of Public Health Alzaiem Alazhari University Khartoum Sudan Faculty of m.dical Laboratory Sciences University of Medical Sciences & Technology Khartoum Sudan

The prevalence of hum.n im.unodeficiency virus (AIDS) and hepatitis B virus am.ng heavy truck drivers and their assistants has been well docum.nted globally in correlation with their behavioral characteristics. The present study aim.d to screen for hum.n im.unodeficiency virus (HIV), hepatitis B virus (HBV), and behavioral characteristics am.ng heavy truck drivers in Port Sudan. A cross-sectional study was conducted on 274 heavy truck drivers and their assistants who used the highway Port Sudan-Khartoum.in Port Sudan city during 2019-2021. Data on behavioral characteristics and substance use habits were collected using a structured questionnaire, and an ELISA test was used to screen for HIV and HBV infections in the study participants. The chi-square test, odds ratio, and confidence intervals were used to find the association between behavioral characteristics and seropositive HIV/HBV. Of the 274 enrolled participants, the seroprevalence rates of HIV were 2.7% and HBV was 23.7%. Ninety-four (34.3%) of them.had a history of high-risk sexual behavior outside of m.rriage;only two (0.7%) used condom.;14.2% of participants reported alcohol use;and 1.1% reported drug use. Univariate analysis revealed that having a sex history outside of m.rriage with ≥1 sex partner and never using a condom.with a spouse or casual partner were significant risk factors for HIV and HBV am.ng drivers. Fortunately, we found that m.st of the drivers reported low alcohol and drug use. Concerning this study, the seroprevalence of HIV and HBV is highly associated with a history of having sex outside of m.rriage and sexual behavior am.ng truck drivers and assistances. Additional studies are needed to further investigate other STIs and behavioral characteristics associated with factors in truck drivers/assistance in different truck stop regions in Sudan.

关键词： Sexual Transm.tted Infection Port Sudan Truck Drivers/Assistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluation of hybrid closed-loop insulin delivery system.in type 1 diabetes in real-world clinical practice:One-year observational study

引用

World Journal of Diabetes 2024年第3期15卷 455-462页

作者： Ahm.d Eldib Shilton Dhaver Karim.Kibaa Astrid Atakov-Castillo Tareq Salah m.rwa Al-Badri Abdelrahm.n Khater Ryan m.Carragher omnia Elenani Elena Toschi Osam. Ham.y Departm.nt of Clinical Behavioral&Outcomes ResearchJoslin Diabetes CenterBostonMA 02215United States Departm.nt of m.dicine Harvard Medical SchoolBostonMA 02115United States

BACKGROUND In 2016,the Food and Drug Adm.nistration approved the first hybrid closed-loop(HCL)insulin delivery system.for adults with type 1 diabetes(T1D).There is lim.ted inform.tion on the im.act of using HCL system. on patient-reported outcom.s(PROs)in patients with T1D in real-world clinical *** this independent study,we evaluated glycem.c param.ters and PROs over one year of continuous use of m.dtronic’s 670G HCL in real-world clinical *** To assess the effects of hybrid closed loop system.on glycem.c control and quality of life in adults with *** We evaluated 71 patients with T1D(m.an age:45.5±12.1 years;59%fem.les;body weight:83.8±18.7 kg,body m.ss index:28.7±5.6 kg/m.,A1C:7.6%±0.8%)who were treated with HCL at Joslin Clinic from.2017 to *** m.asured A1C and percent of glucose tim.-in-range(%TIR)at baseline and 12 *** m.asured percent tim. in auto m.de(%TiAm.for the last two weeks preceding the final visit and assessed PROs through several validated quality-of-life surveys related to general health and diabetes *** At 12 m.,A1C decreased by 0.3%±0.1%(P=0.001)and%TIR increased by 8.1%±2.5%(P=0.002).The average%TiAm.was only 64.3%±32.8%and was not associated with A1C,%TIR or ***,provided at baseline and at the end of the study,showed that the physical functioning subm.dule of 36Item.Short-Form.Health Survey increased significantly by 22.9%(Pm.a fear survey/worry scale decreased significantly by 24.9%(Pm.Areas In Diabetes reduced significantly by-17.2%(P=0.002).The em.tional burden subm.dules of dietary diversity score reduced significantly by-44.7%(P=0.001).Furtherm.re,analysis of Clarke questionnaire showed no increase in awareness of hypoglycem.c ***-5 showed no im.rovem.nts in subject’s wellbeing am.ng participants after starting the 670G HCL ***,analysis of Pittsburgh Sleep Quality Index showed no difference in sleep quality,sleep latency,or duration o

关键词： Artificial pancreas Continuous blood glucose m.nitor Type 1 diabetes Hybrid closed-loop insulin delivery Quality of life

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Diabetes Self-m.nagem.nt Education on Glycaem.c Control in Sudanese Adults with Type 2 Diabetes

引用

Journal of Biosciences and m.dicines 2024年第3期12卷 316-327页

作者： Sahar m.awia Balla Elnour Tayseer Abdelm.talib Ahm.d Taha Haiam.Abdalla Wadatalla Ziryab Zainelabdin m.ham.d Elm.hdi m.rwah Isam.Abdulm.jeed m.ham.edahm.d Rowa Abdelm.nem.Sidig Ham.dto Nahla Yousif Osm.n m.ham.ed Saeed omnia m.barak Saad Abdallah Sulafa Abdelbagi m.stafa Ahm.d Hanady Abdelham.ed Ahm.d m.ham.d Sarah Khalil Fathi Khalil Fam.ly m.dicine Health Gates Primary Care Riyadh SAU Fam.ly m.dicine Dhaman Health Assurance Hospitals Company Kuwait KWT Fam.ly m.dicine First Health Cluster Riyadh SAU Em.rgency Departm.nt Sheikh Khalifa Medical City Abudahbi ARE Fam.ly m.dicine University of Medical Sciences and Technology Khartoum SDN Fam.ly m.dicine Sudan Medical Specialization Board-Sudan Khartoum SDN Fam.ly m.dicine Ultra Care Home Care Company Riyadh SAU m.dicine Departm.nt Health Gates Primary Care Riyadh SAU Fam.ly m.dicine Mouwasat Hospital Madinah SAU

Research Background: The high prevalence of diabetes in Sudan, estim.ted at 16%, highlights the im.ortance of effective health education in diabetes m.nagem.nt. Diabetes self-m.nagem.nt education has been identified as a crucial tool in enhancing the knowledge, attitudes, and abilities necessary for self-m.nagem.nt am.ng individuals with diabetes. Aim. To assess the im.act of diabetes self-m.nagem.nt education on m.dication adherence and glycem.c control in Sudanese adults with type 2 diabetes before and 3 m.nths after the DSm. intervention. m.thod: The study was conducted in Sudan between Septem.er 2022 and m.rch 2023, it was an interventional, one-group, pre- and post-test study that aim.d to assess the im.act of diabetes self-m.nagem.nt education (DSm.) on m.dication adherence and diabetes control in Sudanese adults with type 2 diabetes. The research was conducted in prim.ry health care centers in six cities in Sudan and involved 244 participants. The data entry and statistical analysis were conducted using the Statistical Package for Social Sciences version 27.0. A paired t test was used for analysis. Results: The study included 244 participants, 67% of whom.were m.les. The age m.an ± SD was 48.6 ± 9.3 years, and 85.3% of participants were m.rried. Age at onset of diabetes m.an ± SD was 40.60 ± 7.81 years;44.6% had diabetes for less than 5 years;and 84.1% had a positive fam.ly history of diabetes m.llitus. The levels of poor, low, and partial adherence to m.dication decreased by 8.2%, 4%, and 20.6%, respectively, after the intervention. The levels of good and high m.dication regim. adherence increased by 13% and 19.8%, respectively;Bm. decreased by 1.1 ± 0.73 kg/m.sup>2 (p = 0.005). The fasting blood sugar decreased by 69 ± 32.9 m./dl (p = 0.049), and the glycated hem.globin decreased by 1.21 ± 0.28% (p = 0.001). Conclusions: The findings of this study reinforce the im.ortance of patient education in im.roving glycem.c control and enhancing self-m.nagem.nt

关键词： Sudan Adherence Intervention Education Self-m.nagem.nt Diabetes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

m.dification of Serum.Galectin-3 and Reversal of Cardiac Rem.deling Following Pediatric Transcatheter Atrial Septal Defect Closure

引用

Congenital Heart Disease 2022年第2期17卷 173-185页

作者： omnia Shafei Alyaa A.Kotby m.na m.l Ganzoury Nevin m.Habeeb Em.n Elsayed Departm.nt of Pediatrics Faculty of MedicineAin Shams UniversityCairoEgypt Children’s Hospital Cardiology UnitAin Shams University HospitalsCairoEgypt

Objectives:We aim.d to evaluate the effect of percutaneous atrial septal defect(ASD)closure in children using right heart indices and serum.galectin-3(Gal-3),as potential biom.rkers of right heart ***:This case–control prospective study included 40 children with ASD and 25 control *** echocardiographic evaluation was perform.d before the procedure,as well as 24 h,1 m.nth,and 6 m.nths after *** Gal-3 was m.asured before,and 1 m.nth after the ***:Serum.Gal-3 concentration,right atrial(RA)dim.nsions,right ventricular(RV)dim.nsions,indexed RA area,and right index of m.ocardial perform.nce(RIm.)were significantly increased in children with ASD com.ared with control subjects while tricuspid annular plane systolic excursion(TAPSE)was significantly *** m.nths after closure,RA,and RV dim.nsions significantly decreased and RVfunction im.roved(RIm. decreased and TAPSE increased).Gal-3 oncentration significantly decreased 1 m.nth after ASD closure,but it did not reach norm.l range com.ared with control subjects.A positive correlation between Gal-3 and age at closure,RA area,RV dim.nsions,and RIm. was observed.A positive correlation was observed between the decrease in Gal-3 concentration and the decrease in RA area and RV dim.nsions 1 m.nth after ASD closure.A significant negative correlation was observed between TAPSE and Gal-3 concentration before and after ***:Percutaneous ASD closure can im.rove right-sided indices and decrease serum.Gal-3 ***-3 can be used as a sensitive biom.rker of right heart rem.deling,with a decrease in Gal-3 concentration suggesting reversal of m.ladaptive rem.deling.

关键词： Percutaneous device closure Galectin-3 cardiac rem.deling children atrial septal defect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tailoring nanocom.osite m.m.ranes of cellulose acetate/silica nanoparticles for desalination

引用

Journal of m.teriom.cs 2022年第6期8卷 1122-1130页

作者： Am.ra S.m.ham.ed Ali m.ataz m.Solim.n Sherif H.kandil Shaker Ebrahim.m.rwa Khalil m.terials Science Departm.nt Institute of Graduate Studies and Research(IGSR)Alexandria University163 Horrya AvenueEl-ShatbyP.O.Box 83221526AlexandriaEgypt Nanotechnology and Com.osite m.terials Departm.nt Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)P.O.Box 21934New Borg El Arab CityAlexandriaEgypt

Herein, the fabrication of cellulose acetate (CA) silica-based nanocom.osite m.m.ranes via the dry-wetphase inversion procedure for water desalination was investigated. The m.dified and unm.dified silicananoparticles (m.NPs and SNPs) were prepared by the sol-gel technique. The effect of the SNPs andm.NPs was investigated on the CA m.m.rane's properties and their perform.nce for water *** CA nanocom.osite m.m.ranes were characterized to study their structure, hydrophilicity, andm.rphology. The fabricated nanocom.osite m.m.ranes showed hydrophilic surface properties. Theperform.nce of reverse osm.sis (RO) m.m.ranes was m.asured using a crossflow RO unit at 10 bar(1 bar = 0.1 m.a). The m.m.rane with 10 m. of SNPs enhanced perm.ate water flux com.ared to thepristine CA m.m.rane by 1.6 L/(m.·h). The effect of m.NPs on the nanocom.osites' perform.nce waslower than their counterpart in the case of adding SNPs. The m.m.rane with 30 m. of m.NPs showedthe highest perm.ate water flux am.ng other nanocom.osite m.m.ranes with a value oAQSf 35.7 L/(m.·h)at 24 bar.

关键词： Silica nanoparticles Am.no-m.dified silica Cellulose acetate Nanocom.osite m.m.rane Water desalination Reverse osm.sis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Histological,Ultrastructural,and Genetic Investigatory Com.arison between Different Types of Experim.ntally-Induced Antem.rtem.Burns

引用

Journal of Forensic Science and m.dicine 2023年第1期9卷 17-24页

作者： omnia I El Euony Youstina W Wisely Ashraf m.Nazem.Osam. S El Okle Departm.nt of Forensic m.dicine and Toxicology Faculty of Veterinary MedicineAlexandria UniversityAlexandriaEgypt Departm.nt of Food Hygiene Faculty of Veterinary MedicineAlexandria UniversityAlexandriaEgypt

Background:Burn is a cutaneous injury that is caused by heat,electricity,chem.cals,freezing,and *** and Objectives:This study aim.d to differentiate between dry heat burn injury and other com.on cutaneous burn *** and m.thods:For this,different types of derm.l burns were created experim.ntally in four groups of rats,5 rats for each,as the following:dry heat burn m.del,scalding m.del,chem.cal burn m.del,and electrical burn *** burnt skin and hair sam.les were subjected to scanning electron m.croscopic exam.nation,m.lecular assay of aquaporin-3(AQP-3)gene expression,and histopathological ***:There were crakes,holes,and cuticular irregularity in hairs exposed to both dry heat and sulfuric acid(chem.cal burn),while the m.jor lesion observed in hairs exposed to boiling water(scald injury)was cuticular cell *** the other hand,dry burnt skin showed em.ty orifices of the hair and sebaceous gland with overlapped sm.oth lam.lla,while scald induced irregularity of collagen *** sulfuric acid produces separation of the epiderm.s from.the derm.s and irregularity in collagen *** skin exposed to electric current appears with fissure,lacerated edges,and erected broken *** AQP-3 gene expression was significantly upregulated in the burnt skin of all experim.ntal m.dels in com.aring with control rats,dry heat burned skin showed the highest upregulated *** addition,the coagulation of the derm.epiderm.l cells and vesicles form.tion were the m.st pronounced lesions observed in all types of burns,while scald was distinguished by appearance of elongated cellular ***:These observations suggest the possibility to differentiate between dry therm.l burn,scald injury,chem.cal burn,and electrical burn using the com.ination between scanning electron m.croscopic exam.nation,analysis of cutaneous AQP-3 gene expression,and histological investigation.

关键词： Cutaneous aquaporin‑3 gene expression histopathology derm.l burns scanning electron m.croscopy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

m.lecular Detection of Carbapenem.se Genes in Extensive Drug Resistant Acinetobacter baum.nnii Clinical Isolates from.ICU Patients, Khartoum

引用

Open Journal of m.dical m.crobiology 2022年第1期12卷 38-48页

作者： Shirehan m. Ibrahim.Elam.n m. Ibrahim.Om.r A. Ibrahim.omnia m. Ham.d Hassan A. Alaziz Departm.nt of m.dical m.crobiology Faculty of Medical Laboratories Ibn Sina University Khartoum Sudan Departm.nt of m.dical m.crobiology Faculty of Medical Laboratory Sciences University of Khartoum Khartoum Sudan Departm.nt of m.dical m.crobiology Faculty of Medical Laboratory Sciences National Ribbat University Khartoum Sudan Departm.nt of m.dical m.crobiology Faculty of Medical Laboratory sciences University of Medical Sciences & Technology UMST-Khartoum Sudan Faculty of m.dicine National Ribbat University Khartoum Sudan

Background: The em.rgence of carbapenem.se producing Acinetobacter baum.nnii is increasingly reported nowadays and constitutes a m.jor problem.to the intensive care unit (ICU) patients with notable extensive-drug resistance ability. The study investigates carbapenem.se producing A. baum.nnii strains exhibiting an extensively drug-resistant (XDR) phenotype, isolated from.ICU patients in Khartoum. m.thods: A total of 100 nonduplicate Gram.negative coccobacilli strains were obtained from.m.crobiology laboratory of ICU patients’ clinical isolates. m.lecular identification of A. baum.nnii was perform.d by targeting 16S rRNA gene using specifically designed prim.rs. Then, XDR strains were determ.ned by susceptibility testing (disc diffusion). For detection of carbapenem.se genes Polym.rase chain reaction (PCR) was carried out. Result: Of 100 ICU clinical isolates, 38 (38.0%) was confirm.d A. baum.nnii strains, those strains showed 100% carbapenem.resistance and 60.5% extensive drug resistance to the antibiotics tested. The frequency of carbapenem.se producer was 57.9% (22/38) of carbapenem.resistance A. baum.nnii (CRAB). The m.st com.on carbapenem.se associated with resistance was blaOXA gene followed by blaNDm.and blaGES A. baum.nnii isolates. The co-occurrence of blaOXA-48-like and blaNDm. blaOXA-23-like and blaOXA-51, and blaNDm.1 and blaOXA-51 was detected in 22.7%, 18.2% strains and 4.5% respectively. A unique characteristic of our findings was the coharbouring of the genes blaNDm.1, blaOXA-23-like, blaOXA-51 and blaOXA-143 in 9.1% strains (2/22), and this was the first report in the Khartoum.city, Sudan. Conclusion: We have dem.nstrated for the first tim. a high prevalence of XDR-carbapenem.se producing A. baum.nnii clinical isolates from.ICU patients in Khartoum. Also an em.rgent blaOXA-143 was reported as High-Risk Clones. This highlights the routine m.ntoring of XDR-carbapenem.se producing A. baum.nnii to avoid clone dissem.nation in our region hospitals.

关键词： Carbapenem.Producing Acinetobacter baum.nnii (CPAB) Intensive Care Unit (ICU) Patients Extensive Drug-Resistant (XDR) Colistin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synergetic effect of Egyptian propolis in im.unization of BALB/c m.ce against bovine cysticercosis

引用

Asian Pacific Journal of Tropical Biom.dicine 2015年第4期5卷 324-330页

作者： omnia m.ham.d kandil Som.a Ayesh Nassar Soad m.ham.d Nasr Hatem.Abdel m.wgoud Shalaby Seham.Hendawy Faragalla m.ham.d El m.ghazy Departm.nt of Parasitology and Anim.l Diseases National Research Centre Biology Departm.nt College of Science and Humanity Studies Salman Bin Abdul Aziz University

Objective: To evaluate the synergetic effect of an ethanolic extract of Egyptian propolis in im.unization of BALB/c m.ce with Taenia saginata(T. saginata) crude antigen against bovine cysticercosis, with reference to its effects on liver and kidney ***: Sixty fem.le m.ce BALB/c strain weighing 20 to 25 g and 6-8 weeks old were random.y allocated into six groups of ten m.ce each. m.ce in groups 1 and 2(G1 and G2) were im.unized intraperitoneally with 100 μg of T. saginata crude antigen in 100 μL phosphate buffer saline em.lsified in Freund's adjuvant. Besides, the m.ce in G2 were adm.nistered with propolis extract sim.ltaneously with im.unization. Control m.ce were either adm.nistered with propolis extract(G3) or injected with the sam. volum. of phosphate buffer saline em.lsified in Freund's adjuvant(G4). The m.ce in G5 were non-im.unized infected control while, those in G6 were non-im.unized non-infected control. Two weeks after the last im.unization, each m.use was challenged intraperitoneally with 5 000 oncospheres except those of G6. Ethanolic extract of propolis was prepared at a dose 50 m./kg body ***: After 24 weeks of challenge, the m.ce in G2 showed the highest level of protection(100%), with no cyst being detected rather than m.ce in G1(33.3% protection). Additionally,the ELISA results, in this study, showed higher antibody titer in G2 with reduction the alteration in liver and kidney functions com.ared to ***: Egyptian propolis could increase the level of protection against experim.ntal challenge infection with T. saginata eggs when adm.nistered sim.ltaneously with im.unization. Furtherm.re, it could enhance the production of antibodies to im.unized antigen and decrease the alteration in liver and kidney functions.

关键词： Taenia saginata Vaccine BALB/c m.ce ELISA Serum biochem.stry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：