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Limited genetic diversity among Plasmodium falciparium isolates using nested PCR in Jazan Area, Saudi Arabia

Asian Pacific Journal of Tropical Biomedicine 2015年第5期5卷 407-411页

作者： omima m.eida Amany m.eida Department of Parasitology Faculties of Medicine Suez Canal University Faculties of Pharmacy Jazan University Faculties of medicine Jazan University

Objective: To establish molecular characterization of Plasmodium falciparum field isolates in Jazan Area of Saudi Arabia measured with highly polymorphic genetic marker, i.e. the merozoite surface protein 2(mSP 2).methods: Blood samples were collected from 128 clinically suspected patients attending both Jazan and Sabia hospitals, Kingdom of Saudi Arabia. Both hospitals reflected urban and rural settings respectively. Analysis of central polymorphic region of mSP 2(3D7 and FC27 allelic families) was performed using nested PCR for malaria ***: For mSP 2 allelic families of Plasmodium falciparum, 16 cases(53.3%) carried FC27 type and 14 cases(46.7%) carried 3D7 type, whereas no malaria cases harbored both allelic types. The present study showed that in urban area, 80% of FC27 fragments were 500 bp while in rural area it was 45.5%(P = 0.08). The FC27 400 bp allele was more prevalent in patients from rural than those from the urban area(P = 0.08). The most prevalent infecting 3D7 allele was the 3D7 300 bp in both areas. In the present study, there were no multiple ***: The limited genetic diversity which was observed in Jazan(considered as an endemic area) may be attributed to the small sample size or sustained malaria control program.

关键词： Plasmodium falciparum PCR merozoite surface protein 2

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Immunological Characterization in malaria Patients with and without the Sickle-Cell Trait

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Advances in Infectious Diseases 2014年第3期4卷 152-164页

作者： Howaida A. Nafady Amany m. eida omima m. eida Department of Internal medicine Faculties of Medicine Assuit University Assiut Egypt Jazan University Jazan KSA

Background: malaria is one of the main causes of mortality in tropical zone. Specific immune responses are induced by parasite, including the release of cytokines from peripheral blood mononuclear cells. Sickle cell trait confers a high degree of resistance to severe and complicated malaria. The present study aims to assess immunological response of P. falciparum infection by measuring of total IgG level and IL-6, IL-12 & IL-18 levels for P. falciparum among Saudi Arabian patients with and without sickle-cell trait. Patients and methods: Thirty patients who had clinical suspicion of malaria and sickle-cell trait attending Jazan general hospital in KSA were included in the study. malaria patients with sickle-cell trait will be matched with a control (thirty patients diagnosed to have malaria but without sickle-cell trait). Diagnosis of malaria was done by Immunochromatography strip and blood film. Diagnosis of Sickle-cell trait was done by hemoglobin electrophoresis assessment of total IgG titre and Interleukin 6, 12, 18 levels using ELIZA. Results: Cytokines and IgG in uncomplicated clinical malaria (n = 22) and severe malaria (n = 7) were IL6 (83.1 pg/mL) versus (75.2 pg/mL), IL12 (19.4 pg/mL) versus (16.3 pg/mL), IL18 (22.45 pg/mL) versus (24.2 pg/mL) and IgG (13.3 SD) versus (4.5 SD). Differences in the IL6, IL12 and IgG were statistically significant (p value >0.02, >0.004 & >0.002 respectively). Among malaria patients with sickle cell trait, Cytokines and IgG in asymptomatic (n = 19) and uncomplicated clinical malaria (n = 11) were IL6 (88.9 pg/mL) versus (79.2 pg/mL), IL12 (24 pg/mL) versus (22.9 pg/mL), IL18 (24.2 pg/mL) versus (31.2 pg/mL) and IgG (27 SD) versus (7.35 SD). Differences in IgG were statistically significant (p value ≥ 0.003). Serum IL6 levels were higher in patients with uncomplicated clinical malaria without sickle cell trait (median 83.1 ± 5.1 pg/mL) than in patients with uncomplicated clinical malaria with sickle cell trait (medians 79.2 ±

关键词： malaria Sickle Cell Trait IL-6 IL-12 IL-18 and IgG

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Kinetics of Serum and Local Leukotriene B<sub>4</sub>Response in Experimental Intravaginal Trichomoniasis by <i>T. vaginalis</i>Isolates from Symptomatic and Asymptomatic Women

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Advances in Infectious Diseases 2015年第1期5卷 37-47页

作者： Amany m. eida omima m. eida Ahmed Saied Salem Department of Parasitology Faculty of Medicine Suez Canal University Ismailia Egypt Department of Pathology Faculty of Medicine Al-Azhar University Cairo Egypt

Trichomoniasis is most common sexually transmitted disease caused by T. vaginalis. The clinical manifestation varies from severe manifestation to asymptomatic condition. However, the exact virulence markers led to varied symptomatology was not well clarified. The role of leukotriene B4 (LTB4) in the pathogenesis of many parasitic diseases has been reported. The present study reports the leukotriene B4 levels on different days post infection (3rd, 7th, 14th, 21st and 28th d.p.i.) in serum and vaginal washes (VWs) and vaginal tissues of mice infected intravaginally with T. vaginalis isolates from 10 symptomatic and 10 asymptomatic women. A significant increase in LTB4 was observed on the 3rd to 28th d.p.i. in serum and VWs of mice infected with T. vaginalis isolates from asymptomatic as compared to symptomatic women. The present study also reports the histopathological changes of the posterior vaginal fornix’s and upper portion of the vagina of mice infected intravaginally with T. vaginalis isolates from 10 symptomatic and 10 asymptomatic women. The results show that there are no significant differences between symptomatic and asymptomatic isolates regarding histopathological changes.

关键词： Trichomoniasis Clinical Isolates Experimental Study Pathogenesis Immune Response Leukotriene B4

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Valuation and Determination of Seven and Five Parameters of Photovoltaic Generator by Iterative method

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Smart Grid and Renewable Energy 2016年第9期7卷 247-260页

作者： Ahmed Yahfdhou m. m. menou A. m. Yahya Ne. D. eida A. K. mahmoud Issakha Youm Laboratory of Semiconductors and Solar Energy (LASES) Faculty of Sciences and Technology University of Cheikh Anta Diop Dakar Senegal Applied Research Laboratory of Renewable Energy (LRAER) Faculty of Sciences and Technology University of Sciences Technology and Medicine Nouakchott Mauritania Unit of Electromechanical Research Institute Superior of Technological Education Rosso Mauritania Center of the Study and Research of the Renewable Energy (CERER) Dakar Senegal

The mathematical modeling of solar cells is essential for any optimization operation of the efficiency or the diagnosis of photovoltaic generator. The photovoltaic module is generally represented by an equivalent circuit whose parameters are experimentally calculated by using the characteristic current-tension, I-V. The precise determination of these parameters stays a challenge for the researchers, making to a big difference in the models and the digital methods dedicated to their characterizations. In the present paper, We are interested to characterize the parameters of single diode and two diodes models, in order to plan the behavior of the photovoltaic generator under real functioning conditions. We developed an identification method of the parameters using Newton Raphson method by using the software matlab/Simulink. This method is the faster technique which allows the identification of several parameters and can be used in real time applications. The results of the proposed method show an accordance with the experimental and simulated characteristics of photovoltaic generator.

关键词： Extraction Parameters One and Two Diode model Iterative method Power of Generator PV

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