检索结果-南通市图书馆

npj Computational Materials 2020年第1期6卷 956-963页

作者： Nikolay Borodinov Matthias Lorenz Steven T.King Anton v.Ievlev olga S.Ovchinnikova Center for Nanophase Materials Sciences Oak Ridge National LaboratoryOak RidgeTN 37831USA University of Tennessee KnoxvilleTNUSA

Mass spectrometry imaging(MSI)plays a pivotal role in investigating the chemical nature of complex systems that underly our understanding in biology and *** fields of life science such as proteomics,lipidomics and metabolomics benefit from the ability to simultaneously identify molecules and pinpoint their distribution across a ***,achieving the necessary submicron spatial resolution to distinguish chemical differences between individual cells and generating intact molecular spectra is still a challenge with any single imaging ***,we developed an approach that combines two MSI techniques,matrix-assisted laser desorption/ionization(MALDI)and time-of-flight secondary ion mass spectrometry(ToF-SIMS),one with low spatial resolution but intact molecular spectra and the other with nanometer spatial resolution but fragmented molecular signatures,to predict molecular MSI spectra with submicron spatial resolution.

关键词： modal mass constrained

Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of wholemeal water extract

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2022年第2期10卷 532-549页

作者： Liudmila v.Kozlova Alsu R.Nazipova Oleg v.Gorshkov Liliya F.Gilmullina olga v.Sautkina Natalia v.Petrova Oksana I.Trofimova Sergey N.Ponomarev Mira L Ponomareva Tatyana A.Gorshkova Kazan Institute of Biochemistry and Biophysics Federal Research CenterKazan Scientific Center of the Russian Academy of Sciences420111 KazanRussia Tatar Scientific Research Institute of Agriculture Federal Research CenterKazan Scientific Center of the Russian Academy of Sciences420059 KazanRussia

The grain of rye(Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides,making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water ***, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of m RNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides,whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes.

关键词： Rye(Secale cereale) Kernel development Arabinoxylan Mixed-linkage glucan viscosity

In vivo assessment of structural changes of the urethra in lower urinary tract disease using cross-polarization optical coherence tomography

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2020年第6期3卷 55-70页

作者： Elena B.Kiseleva Alexander A.Moiseev Anton S.Kuyarov Muhammat A.Molvi Grigory v.Gelikonov Anna v.Maslennikova olga S.Streltsova Privolzhsky Research Medical University 10/1 Minin and Pozharsky Square 603005 Nizhny NovgorodRussia Institute of Applied Physics Russian Academy of Sciences Ulyanova Street 46603950Nizhny NovgorodRussia

The paper presents the results of a study of the female urethra in cases of urethral pain syndrome(UPS)and inflammatory diseases of the lower urinary tract using cross-polarization optical coherence tomography(CP OCT).Urethral wall structure was studied in 86 patients;233 CP OCT images were collected.A comparative qualitative analysis of three groups of CP OCT images—"norm","Inflammation"and"UPS"—identified that despite the absence of a clear inflammatory factor in the patient's examination,the urethral tissues in UPS were in an altered *** changes in the urethral wall with UPS and in cases of inflammation were *** a point scale,three experts independently performed visual scoring of the CP OCT *** parameters:epithelial contrast,cavities and the minimum signal depth in the co-channel were *** was found that,individually,the parameters correlate only weakly with the *** under the receiver operating characteristic(ROC)curve was from 0.51 to *** joint use of a number of visual signs has a greater diagnostic value than the use of the criteria *** under the ROC curve using the developed cumulative criterion reached up to *** study could form the basis of a scoring system for assessing the state of the urethral tract using CP OCT images in real *** CP OCT method provides information on the state of urethral tissues that cannot be obtained with traditional cystoscopy.

关键词： Cross-polarization optical coherence tomography urethral pain syndrome connective tissue visual analysis of images automatic classification of images

Phylogenetic relationship and variation of alarm call traits of populations of red-cheeked ground squirrels(Spermophilus erythrogenys sensu lato)suggest taxonomic delineation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2019年第4期14卷 341-353页

作者： vera A.MATROSOvA Anastasia D.IvANOvA Elena v.vOLODINA Ilya A.vOLODIN Dmitry Yu.ALEXANDROv olga v.SIBIRYAKOvA Oleg A.ERMAKOv Engelhardt Institute of Molecular Biology RASMoscowRussia Lomonosov Moscow State University MoscowRussia Moscow Zoo MoscowRussia Severtsov Institute of Ecology and Evolution RASMoscowRussia Department of Zoology and Ecology Penza State UniversityPenzaRussia

Distribution area and taxonomic borders within the species complex Spermophilus erythrogenys sensu lato remain *** evidence suggests that red-cheeked ground squirrels of Southeast Kazakhstan are remarkably different in terms of the acoustic structure of their alarm calls from the red-cheeked ground squirrels of the Kurgan region in *** this study,we analyzed the differences in the acoustic structure of the alarm call and mitochondrial DNA(complete control region,1005-1006 bp and complete cytochrome b gene,1140 bp)in 3 populations of red-cheeked ground squirrels(Tara,Altyn-Emel and Balkhash),all located within areas isolated by geographical barriers in Southeast *** found that the alarm call variables were similar between the 3 study populations and differed by the maximum fundamental frequency(8.46±0.75 kHz)from the values(5.62±0.06 kHz)reported for the red-cheeked ground squirrels from the Kurgan region of Rus*** in mtDNA control region was only 3%and variation in cytochrome b gene was only 2.5%.Phylogenetic trees based on cytochrome b gene polymorphism of 44 individuals from the study area and adjacent territories indicated 3 clades with high(98-100%)bootstrap support:“intermedius,”“brevicauda”and“iliensis”).We conclude that the 3 study populations in Southeast Kazakhstan belong to the clade intermedius and suggest a taxonomical revision of the species complex Spermophilus erythrogenys sensu lato,including analyses of nuclear DNA and alarm calls for populations of the brevicauda and iliensis clades.

关键词： alarm call control region cytochrome b mitochondrial DNA vocal communication

Bacterial melanin promotes recovery after sciatic nerve injury in rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2015年第1期10卷 124-127页

作者： olga.v.Gevorkyan Irina B.Meliksetyan Tigran R.Petrosyan Anichka S.Hovsepyan Institute of Physiology National Academy of Sciences of Armenia Department of Kinesiology Armenian State Institute of Physical Culture Scientific and Production Center "Armbiotechnology" National Academy of Sciences of Armenia

Bacterial melanin, obtained from the mutant strain of Bacillus Thuringiensis, has been shown to promote recovery after central nervous system injury. It is hypothesized, in this study, that bacterial melanin can promote structural and functional recovery after peripheral nerve injury. Rats subjected to sciatic nerve transection were intramuscularly administered bacterial melanin. The sciatic nerve transected rats that did not receive intramuscular administration of bacterial melanin served as controls. Behavior tests showed that compared to control rats, the time taken for instrumental conditioned reflex recovery was significantly shorter and the ability to keep the balance on the rotating bar was significantly better in bacterial melanin-treated rats. Histomor- phological tests showed that bacterial melanin promoted axon regeneration after sciatic nerve injury. These findings suggest that bacterial melanin exhibits neuroprotective effects on injured sciatic nerve, contributes to limb motor function recovery, and therefore can be used for rehabil- itation treatment of peripheral nerve injury.

关键词： nerve regeneration peripheral nerve injury sciatic nerve injury bacterial melanin motor function histomorphology behaviors neural regeneration

Preliminary analyses of the frozen mummies of mammoth(Mammuthus primigenius),bison(Bison priscus)and horse(Equus sp.)from the Yana-Indigirka Lowland,Yakutia,Russia

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Integrative Zoology 2014年第4期9卷 471-480页

作者： Gennady G.BOESKOROv olga R.POTAPOvA Eugeny N.MASHCHENKO Albert v.PROTOPOPOv Tatyana v.KUZNETSOvA Larry AGENBROAD Alexey N.TIKHONOv Diamond and Precious Metal Geology Institute Siberian Branch of Russian Academy of SciencesYakutskRussia The Mammoth Site of Hot Springs Museum Hot SpringsSouth DakotaUSA Paleontological Institute Russian Academy of SciencesMoscowRussia Department of the Mammoth Fauna Studies Academy of Sciences of the Sakha(Yakutia)Republic YakutskRussia M.v.Lomonosov Moscow State University MoscowRussia Zoological Institute Russian Academy of SciencesSt.PetersburgRussia

The frozen bodies of a young woolly mammoth(Mammuthus primigenius),a wild horse(Equus sp.)and a steppe bison(Bison priscus)were recently found in the northern Yakutia(northeastern Siberia).All specimens have preserved bones,skin and soft *** the woolly mammoth and the Pleistocene horse were represented by partial frozen bodies,the steppe bison body was recovered in an absolutely complete *** specimens were found frozen in the permafrost,with some of the tissues *** wild horse and steppe bison are of Holocene age,and the mammoth is of Late Pleistocene age.

关键词： bison horse mammoth mummy Siberia

Continuous wafer-scale graphene on cubic-SiC（O01）

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2013年第8期6卷 562-570页

作者： Alexander N. Chaika olga v. Molodtsova Alexei A. Zakharov Dmitry Marchenko Jaime Sanchez-Barrig Andrei varykhalov Igor v. Shvets victor Yu. Aristov Institute of Solid State Physics RAS Chemogolovka Moscow District 142432 Russia Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN) School of Physics Trinity College Dublin 2 Ireland HASYLAB at DESY D-22607 Hamburg Germany MAX-lab Lund University Box 118 22100 Lund Sweden Helmholtz-Zentrum Berlin für Materialien und Energie D- 12489 Berlin Germany

The atomic and electronic structure of graphene synthesized on commercially available cubic-SiC（001）/Si（001） wafers have been studied by low energy electron microscopy （LEEM）, scanning tunneling microscopy （STM）, low energy electron diffraction （LEED）, and angle resolved photoelectron spectroscopy （ARPES）. LEEM and STM data prove the wafer-scale continuity and uniform thickness of the graphene overlayer on SIC（001）. LEEM, STM and ARPES studies reveal that the graphene overlayer on SIC（001） consists of only a few monolayers with physical properties of quasi-freestanding graphene. Atomically resolved STM and micro-LEED data show that the top graphene layer consists of nanometer-sized domains with four different lattice orientations connected through the 〈110〉-directed boundaries. ARPES studies reveal the typical electron spectrum of graphene with the Dirac points close to the Fermi level. Thus, the use of technologically relevant SiC（001）/Si（001） wafers for graphene fabrication repre-sents a realistic way of bridging the gap between the outstanding properties of graphene and their applications.

关键词： graphene cubic-SiC(001) STM ARPES LEEM LEED

Application of pan-sharpening algorithm for correlative multimodal imaging using AFM-IR

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

npj Computational Materials 2019年第1期5卷 714-722页

作者： Nikolay Borodinov Natasha Bilkey Marcus Foston Anton v.Ievlev Alex Belianinov Stephen Jesse Rama K.vasudevan Sergei v.Kalinin olga S.Ovchinnikova Center for Nanophase Materials Science Oak Ridge National LaboratoryOak RidgeTN 37831USA Department of Energy Environmental&Chemical EngineeringWashington University in St.LouisOne Brookings DriveSt.LouisMO 63130USA

The coupling of atomic force microscopy with infrared spectroscopy(AFM-IR)offers the unique capability to characterize the local chemical and physical makeup of a broad variety of materials with nanoscale ***,in order to fully utilize the measurement capability of AFM-IR,a three-dimensional dataset(2D map with a spectroscopic dimension)needs to be acquired,which is prohibitively time-consuming at the same spatial resolution of a regular AFM *** this paper,we provide a new approach to process spectral AFM-IR data based on a multicomponent pan-sharpening *** approach requires only a low spatial resolution spectral and a limited number of high spatial resolution single wavenumber chemical maps to generate a high spatial resolution hyperspectral image,greatly reducing data acquisition *** a result,we are able to generate highresolution maps of component distribution,produce chemical maps at any wavenumber available in the spectral range,and perform correlative analysis of the physical and chemical properties of the *** highlight our approach via imaging of plant cell walls as a model system and showcase the interplay between mechanical stiffness of the sample and its chemical *** believe our pan-sharpening approach can be more generally applied to different material classes to enable deeper understanding of that structure-property relationship at the nanoscale.

关键词： utilize modal walls

Detection of angiospastic disorders in the microcirculatory bed using laser diagnostics technologies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2018年第1期11卷 65-79页

作者： Irina N.Makovik Andrey v.Dunaev victor v.Dremin Alexander I.Krupatkin viktor v.Sidorov Lyudmila S.Khakhicheva vadim F.Muradyan olga v.Pilipenko Ilya E.Rafailov Karina S.Litvinova Biomedical Photonics Instrumentation Group Scientic-Educational Centre of"Biomedical Engineering"Orel State University named after I.S.Turgenev Orel 302026 Russian Federation Priorov Central Research Institute of Traumatology and Orthopaedics Moscow 127299 Russian Federation SPE"LAZMA"Ltd Moscow 125252Russian Federation Orel Regional Clinical Hospital Orel 302028 Russian Federation School of Engineering and Applied Sciences Aston Institute of Photonic Technologies Aston University BirminghamB47ETUK Optoelectronics and Biomedical Photonics Group Aston Institute of Photonic Technologies Aston University BirminghamB47ETUK Aston Medical School Aston University BirminghamB47ETUK

The evaluation of the microcirculatory bed functional state and the identification of angiospastic disorders with related complications,when the pathological changes are reversible,have an important role in medical *** aim of this study was to evaluate the possibility of using optical noninvasive methods and the cold pressor test to solve this problem.A total of 33 patients with rheumatological diseases and 32 healthy volunteers were included in the *** Doppler flowmetry,tissue reflectance oximetry and pulse oximetry were used as optical noninvasive *** parameters were recorded before,immediately after and 20 min after the cold pressor *** on the measured parameters,the complex parameters of the microcirculatory bed were calculated.A detailed statistical analysis of the parameter changes for each individual in the two groups displayed diverse microcirculatory bed parameter responses upon cold exposure,with differing recovery of parameters after *** diagnostic criteria were proposed for the identification of angiospastic *** to the proposed criteria,27 people of the volunteers group were confirmed to not display any *** the patient group,however,18 people were observed to have a relatively normal functional state of the microcirculatory bed,while 15 people were observed to have a possible tendency to *** highlight the diferences between a relatively normal state and presence of angiospastic disorders,statistical analysis of experimental data was carried out,which revealed significant *** analysis of data with angiospastic disorders identified a relationship between their diagnoses and the results of labo-ratory ***,the evaluation of combined noninvasive optical diagnostic method use,the cold pressor test and proposed diagnostic criteria showed a positive *** approach can be used to detect the presence of possible angiospastic disorders and related complications,as

关键词： Noninvasive diagnostics laser Doppler flowmetry tissue relctance oximetry pulse oximetry cold pressor test microcirculatory bed angiospasm

Extended Corey-Chaykovsky reactions:transformation of 2-hydroxychalcones to benzannulated 2,8-dioxabicyclo[3.2.1]octanes and 2,3-dihydrobenzofurans

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Organic Chemistry Frontiers 2022年第3期9卷 737-744页

作者： Alexander A.Fadeev Anton S.Makarov olga A.Ivanova Maxim G.Uchuskin Igor v.Trushkov Department of Chemistry Perm State UniversityBukireva 15Perm 614990Russian Federation Department of Chemistry M.V.Lomonosov Moscow State UniversityLeninskie Gory 1-3Moscow 119991Russian Federation N.D.Zelinsky Institute of Organic Chemistry Leninsky pr.47Moscow 119334Russian Federation. D.Rogachev National Medical Research Center of Pediatric Hematology Oncology and ImmunologySamory Mashela 1Moscow 117997Russian Federation

We report the divergent synthesis of benzannulated 2,8-dioxabicyclo[3.2.1]octanes and 2,3-dihydrobenzofurans using the concept of extended Corey-Chaykovsky *** this concept,2-hydroxychalcones were treated with the Corey ylide providing highly reactive donor-acceptor cyclopropanes that were introduced in a one pot manner for further transformations.

关键词： octane donor