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GTR-Mediated Radial Import Directs Accumulation of Defensive Glucosinolates to Sulfur-Rich Cells in the Phloem Cap of Arabidopsis Inflorescence Stem

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Molecular Plant 2019年第11期12卷 1474-1484页

作者： Deyang Xu Pascal Hunziker olga koroleva Andreas Blennow Christoph Crocoll Alexander Schulz Hussam Hassan Nour-Eldin Barbara Ann Halkier DynaMo Center Department of Plant and Environmental SciencesFaculty of ScienceUniversity of Copenhagen1871 Frederiksberg CDenmark Lonza Biologies Pic 228 Bath RoadSlough SL14DXUK Section for Plant Glycobiology Department of Plant and Environmental SciencesFaculty of ScienceUniversity of Copenhagen1871 Frederiksberg CDenmark

In the phloem cap region o i Arabidopsis plants,sulfur-rich cells(S-cells)accumulate>100 mM glucosinolates(GLS),but are biosynthetically *** source and route of S-cell-bound GLS remain *** this study,using single-cell sampling and scanning electron microscopy with energy-dispersive X-ray analysis we show that two GLS importers,NPF2.10/GTR1 and NPF2.11/GTR2,are critical for GLS accumulation in S-cells,although they are not localized in the *** of GLS levels in S-cells in multiple combinations of homo-and heterografts o lg t r l gtr2,biosynthetic null mutant and wild-type plants indicate that S-cells accumulate GLS via symplasmic connections either directly from neighboring biosynthetic cells or indirectly to non-neighboring cells expressing GTR1/*** sources and transport routes exist for different types of GLS,and vary depending on the position of S-cells in the inflorescence *** on these findings,we propose a model illustrating the GLS transport routes either directly from biosynthetic cells or via GTR-mediated import from apoplastic space radially into a symplasmic domain,wherein the S-cells are the ultimate ***,we observed accumulation of the cyanogenic glucoside defensive compounds in high-turgor cells in the phloem cap of Lotus japonicus,suggesting that storage of defensive compounds in high-turgor cells may be a general mechanism for chemical protection of the phloem cap.

关键词： Arabidopsis glucosinolate S-cell phloem cap xylem symplasmic transport

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Overexpression of the recombinant xyloglucanase <i>sp-Xeg</i>from <i>Penicillium canescens</i>accelerates growth and rooting of transgenic aspen plants

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Natural Science 2013年第6期5卷 36-43页

作者： Vidyagina Elena Kovalitskaya Yulia Loginov Dmitry koroleva olga Shestibratov Konstantin Branch of Shemyakin Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Pushchino Russia

Analysis of the properties of transgenic aspen clones with recombinant gene xyloglucanase sp-Xeg from fungi Penicillium canescens showed the presence of complex modifications both in the wood and the phenotype of plants. Biometric analysis revealed an increase in the height of transgenic plants as compared to control plants. Increasing in the height of the shoot of 24.8%, 25% and 26% was observed for lines PtXIVXeg1a, PtXVXeg1a, PtXVXeg1b, respectively. Also there was an increase in the number of internodes in some transgenic clones. For the first time we showed the change in plants rhizogenesis with the recombinant gene xyloglucanase. In 10 of the 25 lines the rooting efficiency in vitro exceeded the control value. The maximum value of the rhizogenesis was fixed for line PtXVXeg1a (2.5 times higher than the control value). The mass of the root system for 6 of the 25 clones in the greenhouse was higher by 20% than the control value. The pentosan content decrease was also detected in all wood samples of transgenic plants. The obtained data of xyloglucanase activity and pentosan content generally correlated with phenotypic modifications.

关键词： Populus Tremula Activity of Xyloglucanase Pentosans Efficiency of Rooting

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Interaction of Coal Humic Acids with Fungal Laccase

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Advances in Microbiology 2013年第2期3卷 145-153页

作者： Natalia A. Kulikova Valentina N. Davidchik Eugenia A. Tsvetkova olga V. koroleva Department of Soil Science M.V. Lomonosov Moscow State University Moscow Russia

Humic acids (HA) are one of the main environmental factors controlling the fate and behavior of the compounds released into the environment. In particular, they are universally considered of great importance in determining soil extracellular enzyme activity and stability via association with essential soil enzymes. The objective of this study was to investigate the interaction of coal HA with an extracellular multicopper oxidase laccase (EC 1.***.3.2) that catalyze the oxidation of a wide range of reducing substances in the environment. Using size-exclusion chromatography analysis and monitoring laccase activity, the formation of a stable and an enzymatically active complex between HA and laccase was shown. Basing the data obtained by isoelectric focusing of HA-laccase complex, non-covalent character of laccase association with HA was considered and binding of laccase to HA by weak dispersive forces such as van der Waals, hydrophobic, π-π, CH-π and others was hypothesized.

关键词： Humic Acids Laccase Activity Size-Exclusion Chromatography Complexes Isoelectric Focusing

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