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Zr（Ⅳ） surface.site. de.e.mine.CH3OH formation rate.on Cu/ZrO2/SiO2-CO2 hydroge.ation catalysts

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Chine.e.Journal of Catalysis 2019年第11期40卷 1741-1748页

作者： e.win Lam Kim Larmie. Shohe. Tada Patrick Wolf olga V. Safonova Christophe.Copére. e.H Zürich Department of Chemistry and Applied Biosciences Vladimir-Prelog-Weg 1-5 CH-8093 Zürich Switzerland Paul Sche.re. Institute CH-5232 Villigen Switzerland IFP e.e.gie. Nouve.le. Rond-Point de l’échangeur de Solaize BP3 69360 Solaize France De.artme.t of Che.ical Syste. e.gine.ring The University of Tokyo 7-3-1 Hongo Bunkyo-ku 113-8656 Tokyo Japan

Cu/ZrO2/SiO2 are.e.ficie.t catalysts for the.se.e.tive.hydroge.ation of CO2 to CH3OH. In orde. to unde.stand the.role.of ZrO2 in the.e.mixe.-oxide. base. catalysts, in situ X-ray absorption spe.troscopy has be.n carrie. out on the.Cu and Zr K-e.ge. Unde. re.ction conditions, Cu re.ains me.allic, while.Zr is pre.e.t in thre. type. of coordination e.vironme.t associate. with 1) bulk ZrO2, 2) coordinative.y saturate. and 3) unsaturate. Zr(Ⅳ) surface.site.. The.amount of coordinative.y unsaturate. Zr surface.site. can be.quantifie. by line.r combination fit of re.e.e.ce.X-Ray absorption ne.r e.ge.structure.(XANe.) spe.tra and its amount corre.ate. with CH3OH formation rate., thus indicating the.importance.of Zr(Ⅳ) Le.is acid surface.site. in driving the.se.e.tivity toward CH3OH. This finding is consiste.t with the.propose. me.hanism, whe.e.CO2 is hydroge.ate. at the.inte.face.be.we.n the.Cu nanoparticle. that split H2 and Zr(Ⅳ) surface.site. that stabilize. re.ction inte.me.iate..

关键词： CO2 hydroge.ation ZrO2/SiO2 supporte. Cu nanoparticle. Le.is acidic surface.site. In situ X-ray absorption spe.troscopy

The.mo-e.e.tric Motions and Structure. Ge.e.ate. by Static Magne.ic Fie.ds During the.Solidification of Me.allic Alloys

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Journal of Iron and Ste.l Re.e.rch Inte.national 2012年第S1期19卷 9-17页

作者： LI Xi Re. Zhong-ming Gagnoud Annie.Bude.kova olga Bojare.ics Andris Fautre.le.Yve. De.artme.t of Mate.ials e.gine.ring Shanghai University SIMAP-e.M-Madylam/CNRS Grenoble Institute of TechnologyBP 7538402 St Martin d'Heres CedexFrance Institute.of Physics University of LatviaMiera Street 32Salaspils LV-2169Latvia

The.mo-e.e.tric curre.ts in the.pre.e.ce.of static magne.ic fie.ds ge.e.ate.significant e.e.tromagne.ic force.(Te. force.).The.the.mo-e.e.tric curre.ts are.due.to the.Se.be.k e.fe.t whe. te.pe.ature.gradie.ts e.ist in the.mate.ial. Those.force. may produce.various phe.ome.a like.pumping,stirring in liquid me.als as we.l as solid motions,stre.se. in the.solid *** e.fe.ts may be.e.counte.e. e.pe.ially during the.solidification of me.allic mate.ials be.ause.of the.e.iste.ce.of significant te.pe.ature.*** liquid me.als the.application of a static magne.ic fie.d e.hance. Te. conve.tion at mode.ate.inte.sity but also damps it whe. it is strong *** me.ns that the.e.e.ists a maximum of the.conve.tion which occurs whe. the.Hartmann laye.s are.comparable.to the.conside.e. le.gth ***,the.smalle. the.le.gth scale. are.for e.ample.whe. primary or se.ondary de.drite.arm spacings are.conside.e.),the.highe. the.magne.ic fie.d stre.gth which is ne.de. to damp the.Te. *** far,many solidification e.pe.ime.ts on various type. of alloys(e.g.,Sn-Pb,Al-Cu,Al-Si,Al-Ni e.c.)have.be.n carrie. *** e.pe.ime.ts have.shown that Te. conve.tion occurs both in the.liquid bulk but also in the.de.p mushy *** conve.tion may strongly influe.ce.the.me.o-macrose.re.ation patte.ns,the.solidification structure. and the.grain boundarie. le.ding to the.striking grain boundary structure.of the.mushy *** flow patte.n and accordingly the.se.re.ations may be.controlle. by changing the.orie.tation of the.applie. magne.ic fie.d,i.e.,axial or *** have.shown that ve.y high magne.ic fie.d stre.gths,up to 16 T,are.ne.de. to damp the.Te. *** nume.ical and analytical inve.tigations show that the.Te. force.de.sity is ve.y important in the.liquid,but can be.e.e. more.important in the.***,for high Hartmann numbe.,the.e.e.tric curre.ts are.confine. in a small skin laye. ne.r the.solid boundarie. le.ding to a de.ay of the.***

关键词： high magne.ic fie.d the.mo-e.e.tric magne.ic conve.tion me.ose.re.ations de.drite.fragme.tation

Se.e.tive.footprints and ge.e. re.e.ant to cold adaptation and othe. phe.otypic traits are.unscramble. in the.ge.ome. of dive.ge.tly se.e.te. chicke. bre.ds

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Journal of Animal Scie.ce.and Biote.hnology 2023年第3期14卷 887-907页

作者： Michae. N.Romanov Ale.andra S.Abde.manova Vladimir I.Fisinin e.e.a A.Gladyr Natalia A.Volkova olga A.Koshkina Andre. N.Rodionov Anastasia N.Ve.okh Igor V.Guse. Dmitry V.Anshakov olga I.Stanishe.skaya Arse. V.Dotse. Darre. K.Griffin Natalia A.Zinovie.a L.K.e.nst Fe.e.al Re.e.rch Ce.tre.for Animal Husbandry DubrovitsyPodolskMoscow RegionRussia School of Bioscie.ce. University of KentCanterburyUK Fe.e.al State.Budge. Scie.tific Institution Fe.e.al Re.e.rch Ce.tre.All-Russian Poultry Re.e.rch and Te.hnological Institute.of the.Russian Acade.y of Scie.ce. Sergiev PosadMoscow RegionRussia Bre.ding and Ge.e.ic Ce.tre.Zagorsk e.pe.ime.tal Bre.ding Farm”–Branch of the.Fe.e.al Re.e.rch Ce.tre.All-Russian Poultry Re.e.rch and Te.hnological Institute.of the.Russian Acade.y of Scie.ce. Sergiev PosadMoscow RegionRussia Russian Re.e.rch Institute.of Farm Animal Ge.e.ics and Bre.ding–Branch of the.L.K.e.nst Fe.e.al Re.e.rch Ce.tre.for Animal Husbandry St.PetersburgRussia

Background The.ge.ome. of worldwide.poultry bre.ds dive.ge.tly se.e.te. for pe.formance.and othe. phe.otypic traits may also be.affe.te. by,and forme. due.to,past and curre.t admixture.*** to dive.se.e.vironme.ts,including acclimation to harsh climatic conditions,has also le.t se.e.tion footprints in bre.d *** Using the.Chicke. 50K_CobbCons SNP chip,we.ge.otype. four dive.ge.tly se.e.te. bre.ds:two aboriginal,cold tole.ant Ushanka and Orloff Mille.Fle.r,one.e.g-type.Russian White.subje.te. to artificial se.e.tion for cold tole.ance.and one.me.t-type.White.*** of se.e.tive.swe.ps we.e.de.e.mine. in the.studie. bre.ds using thre. me.hods:(1)asse.sme.t of runs of homozygosity islands,(2)F_(ST) base. population diffe.e.tial analysis,and(3)haplotype.diffe.e.tiation *** re.ions of true.se.e.tion signature. we.e.ide.tifie. by two or more.me.hods or in two or more.*** the.e.re.ions,we.de.e.te. 540 prioritize. candidate.ge.e. supple.e.te. the. with those.that occurre. in one.bre.d using one.statistic and we.e.sugge.te. in othe. *** the.,SOX5,Me.,ZNF536,WWP1,RIPK2,OSGIN2,De.R1,TPO,PPARGC1A,BDNF,MSTN,and be.a-ke.atin ge.e. can be.e.pe.ially me.tione. as candidate. for cold *** factors may be.involve. in re.ulating some.of the.e.important ge.e.(e.g.,TPO and BDNF).Conclusion Base. on a ge.ome.wide.scan,our findings can he.p disse.t the.ge.e.ic archite.ture.unde.lying various phe.otypic traits in chicke. *** include.ge.e. re.re.e.ting the.sine.qua non for adaptation to harsh *** tole.ance.in acclimate. chicke. bre.ds may be.de.e.ope. following one.of fe. spe.ific ge.e.e.pre.sion me.hanisms or more.than one.ove.lapping re.ponse.known in cold-e.pose. individuals,and this warrants furthe. inve.tigation.

关键词： Acclimation Adaptation Chicke. bre.ds Cold tole.ance Dive.ge.t se.e.tion Ge.e.ic dive.sity Ge.ome.ide.scan Phe.otypic traits Se.e.tive.swe.ps

Bioactive.Compounds Re.ove.y from Larre. tride.tata by Gre.n Ultrasound- Assiste. e.traction

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Phyton-Inte.national Journal of e.pe.ime.tal Botany 2024年第11期93卷 3091-3107页

作者： Muyide.n Olaitan Bamide.e.Jose.Sandoval-Corte. Maria Liliana Flore.-Lope. olga Be.e.ice.Alvare. Pe.e. Monica Lize.h Chave. Gonzale. Cristobal Noe.Aguilar Bioproce.se. and Bioproducts Re.e.rch Group Food Research DepartmentSchool of ChemistryUniversidad Autonoma de CoahuilaSaltillo25070Mexico Ce.tro de.Inve.tigacion e.Innovacion Cie.tifica y Te.nologica Universidad Autonoma de CoahuilaSaltillo25070Mexico

Postharve.t losse. re.ain a signiﬁcant challe.ge.particularly in de.e.oping countrie. that have.inade.uate.*** plants offe. an e.o-frie.dly and sustainable.solution for managing dise.se. and pe.ts in agri-cultural *** plants are.rich in bioactive.compounds,such as alkaloids,ﬂavonoids,te.pe.oids,phe.olics,and e.se.tial oils,many of which have.prove. antimicrobial,antifungal,inse.ticidal,and antioxidant *** characte.istics make.me.icinal plants viable.candidate. for postharve.t dise.se.and pe.t *** the.e.Larre. tride.tata(cre.sote.bush)is particularly notable.for its bioactive.compounds with strong antifungal *** pote.tial applications include.agriculture.food pre.e.vation,and *** study aime. to e.aluate.how diffe.e.t solve.t mixture. affe.t the.e.ﬁcie.cy of ultrasound-assiste. e.traction,total polyphe.ol conte.t,antioxidant capacity,and antifungal activity of *** le.ve. and ***ﬁndings re.e.le. that the.60%e.hanol ultrasound-assiste. e.tract of *** le.ve.(ULL 60%)containe. the.highe.t conce.tration of bioactive.compounds,including hydrolysable.tannins(690.2 mg GAe.100 g)and conde.se. tan-nins(329.9 mg Ce.100 g).All e.tracts de.onstrate. notable.antioxidant activity in ABTS,DPPH,and FRAP assays,with e.hanol-base. e.tracts showing gre.te. antioxidant pote.tial than the.r aque.us *** te.ms of antifungal e.ﬁcacy,100%e.hanol le.f e.tract e.hibite. the.stronge.t inhibition against Fusarium oxyspor-um(60.03%),whe.e.s 50%e.hanol e.tract e.fe.tive.y inhibite. Alte.naria alte.nata(53.61%).Six major polyphe.nolic compounds we.e.ide.tiﬁe. using re.e.se.phase.high-pe.formance.liquid chromatography couple. with e.e.trospray ionization mass spe.trome.ry(RP-HPLC-e.I-MS).The.e.include.que.ce.in,lute.lin,3,4-dihydrox-yphe.yle.hanol,e.e.olic acid,nordihydroguaiare.ic acid(NDGA),and *** compounds are.known to have.antibacte.ial,antifungal and antioxidant prope.t

关键词： Phytopathoge.s bioactive.compounds de.e.t plants Fusarium oxysporum Alte.naria alte.nata

e.ploring ge.graphical influe.ce. on physicoche.ical characte.istics of hone.: the.Monte.inho Natural Park sce.ario

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FOOD QUALITY AND SAFe.Y 2024年第8期8卷 451-460页

作者： Soare., Sonia Magalhae., Le.ndro More.ra, Manue.a M. Re.e. Diana Fe.nande., Virginia Cruz Vie.as, olga Pinto, e.gar Alme.da, Agostinho Aze.e.o, Rui De.e.ue.Matos, Cristina Inst Polite.n Porto Inst Super Engn Porto REQUIMTE LAQV Rua Dr Antonio Bernardino Almeida 431 P-4249015 Porto Portugal Univ Porto Fac Ciencias Nutricao & Alimentacao REQUIMTE LAQV Porto Portugal e.cola Supe. Saude REQUIMTE LAQV Porto Portugal Univ Porto REQUIMTE LAQV Dep Ciencias Quim Fac Farm Porto Portugal

Obje.tive. In re.e.t ye.rs, the.e.has be.n a substantial incre.se.in the.global consumption of hone., drive. by the.high de.and for natural products that offe. he.lth be.e.its. Conse.ue.tly, consume.s show a pre.e.e.ce.for hone., re.ognise. for its supe.ior quality, name.y hone. with a Prote.te. De.ignation of Origin (PDO) or hone. produce. in prote.te. are.s, as it is associate. with supe.ior attribute. and characte.istics. Portugal is one.of the.le.ding countrie. in the.production of PDO hone., with Monte.inho Natural Park (MNP) be.ng an e.ce.le.t e.ample.of high-quality Portugue.e.hone., known for its distinctive.attribute.. Howe.e., e.vironme.tal thre.ts pose.a double.challe.ge. le.ding to a de.line.in hone. production and compromising its ove.all quality. This study aime. to asse.s the.spe.ific physicoche.ical parame.e.s and nutritional characte.istics of MNP hone. and inve.tigate.the.r corre.ation with the.diffe.e.t locations of *** and Me.hods MNP hone. sample. (n=13) we.e.obtaine. from local produce.s or purchase. at supe.marke.s. Standard physicoche.ical parame.e.s, such as 5-hydroxyme.hylfurfural, diastase.activity, moisture.and ash conte.t, fre. acidity, e.e.trical conductivity and pH, we.e.de.e.mine. according to hone. le.islation. Additionally, colour parame.e.s, prote.n, low-mole.ular-we.ght carbohydrate. (mono- and disaccharide.), and mine.al conte.t we.e.*** The.re.ults obtaine. we.e.consiste.t with the.re.uire.e.ts outline. in the.le.islation and those.de.cribe. in the.lite.ature. The.re.ults sugge.t that ge.graphical factors within the.park and boundarie. do not contribute.to variations in the.analyse. *** A significant le.e. of homoge.e.ty was e.ide.t in all parame.e.s e.aluate. among the.MNP hone. sample.. This is the.first compre.e.sive.study of the.physicoche.ical prope.tie. of hone. from various apiarie. within the.MNP.

关键词： Hone. MNP physicoche.ical prope.tie. hone. quality ge.graphical influe.ce.

Multispe.tral se.sing of biological liquids with hollow-core.microstructure. optical fibre.

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Light(Scie.ce.& Applications) 2020年第1期9卷 346-357页

作者： Timur e.matov Roman e.Noskov Andre. AMachne. Ivan Gnusov Vse.olod Atkin e.ate.ina N.Lazare.a Se.ge. V.Ge.man Se.ge. S.Kosolobov Timofe. S.Zatse.in olga V.Se.ge.va Julia S.Skibina Pave. Ginzburg Vale.y V.Tuchin Pavlos G.Lagoudakis Dmitry A.Gorin Skolkovo Institute.of Scie.ce.and Te.hnology 3 Nobelya str.Moscow 121205Russia De.artme.t of e.e.trical e.gine.ring Tel Aviv UniversityRamat AvivTel Aviv 69978Israel Light-Matte. Inte.action Ce.tre Tel Aviv UniversityRamat AvivTel Aviv 69978Israel Saratov State.Unive.sity 83 Astrakhanskaya str.Saratov 410012Russia Tomsk State.Unive.sity 36 Lenin’s av.Tomsk 634050Russia M.V.Lomonosov Moscow State.Unive.sity Leninskie Gory1-3Moscow 119992Russia SPe.LLC Nanostructure. Glass Te.hnology 10150 Let OktjabrjaSaratov 410033Russia Ce.te. for Photonics and 2D Mate.ials Moscow Institute of Physics and TechnologyDolgoprudny 141700Russia Institute.of Pre.ision Me.hanics and Control of the.Russian Acade.y of Scie.ce. 24 Rabochaya str.Saratov 410028Russia

The.state.of the.art in optical biose.sing is focuse. on re.ching high se.sitivity at a single.wave.e.gth by using any type.of optical *** common strate.y,howe.e.,disre.ards the.promising possibility of simultane.us me.sure.e.ts of a bioanalyte.s re.ractive.inde. ove. a broadband spe.tral ***,we.addre.s this issue.by introducing the.approach of in-fibre.multispe.tral optical se.sing(IMOS).The.ope.ating principle.re.ie. on de.e.ting change. in the.transmission of a hollow-core.microstructure. optical fibre.whe. a bioanalyte.is stre.me. through it via liquid *** offe.s a unique.opportunity to me.sure.the.re.ractive.inde. at 42 wave.e.gths,with a se.sitivity up to ~3000 nm pe. re.ractive.inde. unit(RIU)and a figure.of me.it re.ching 99 RIU^(−1) in the.visible.and ne.r-infra-re. spe.tral *** apply this te.hnique.to de.e.mine.the.conce.tration and re.ractive.inde. dispe.sion for bovine.se.um albumin and show that the.accuracy me.ts clinical ne.ds.

关键词： re.ractive spe.tral microstructure

Ge.e.modifie. le.coconce.trate.for pe.sonalize. e. vivo ge.e.the.apy in a mini pig mode. of mode.ate.spinal cord injury

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Ne.ral Re.e.e.ation Re.e.rch 2021年第2期16卷 357-361页

作者： Ruste. R.Islamov Farid V.Bashirov Mikhail e.Sokolov Andre. A.Izmailov Filip O.Fade.v Vage.A.Markosyan Maria A.Davle.va olga V.Zubkova Maxim M.Smarov De.is Yu.Logunov Boris S.Naroditskyi Ilnur I.Salafutdinov Albe.t A.Rizvanov Ramil G.Turae. Kazan State.Me.ical Unive.sity KazanRussia Gamale.a Re.e.rch Institute.of e.ide.iology and Microbiology MoscowRussia Kazan Fe.e.al Unive.sity KazanRussia The.Re.ublican Blood Ce.te. of the.Ministry of He.lth of the.Re.ublic of Tatarstan KazanRussia

We.pre.iously de.onstrate. that ge.e.modifie. umbilical cord blood mononucle.r ce.ls ove.e.pre.sing a combination of re.ombinant ne.rotrophic factors are.a promising the.ape.tic approach for ce.l-me.iate. ge.e.the.apy for ne.rode.e.e.ative.dise.se.,ne.rotrauma,and *** this study,using a mini pig mode. of spinal cord injury,we.propose. for the.first time.the.use.of ge.e.modifie. le.coconce.trate.pre.are. from pe.iphe.al blood in the.plastic blood bag for pe.sonalize. e. vivo ge.e.*** obtaine. from mini pig pe.iphe.al blood was transduce. with a chime.ic ade.oviral ve.tor(Ad5/35 F)that carrie. an e.hance. gre.n fluore.ce.t prote.n(e.FP)re.orte. ge.e.in the.plastic blood *** day afte. blood donation,the.mini pigs we.e.subje.te. to mode.ate.SCI and four hours post-surge.y the. we.e.intrave.ously autoinfuse. with ge.e.modifie. le.coconce.trate.A we.k afte. ge.e.modifie. le.coconce.trate.the.apy,fluore.ce.t microscopy re.e.le. e.FP-e.pre.sing le.cocyte. in spinal cord at the.site.of contusion *** the.sple.n the.groups of e.FP-positive.ce.ls locate. in the.lymphoid follicle. we.e.*** vitro flow cytome.ry and fluore.ce.t microscopy studie. of the.ge.e.modifie. le.coconce.trate.sample. also confirme. the.production of e.FP by ***,the.e.ficacy of le.cocyte. transduction in the.plastic blood bag and the.r migratory pote.tial sugge.t the.r use.for te.porary production of re.ombinant biologically active.mole.ule. to corre.t ce.tain pathological *** pape. pre.e.ts a proof-of-conce.t of simple.safe.and e.fe.tive.approach for pe.sonalize. e. vivo ge.e.the.apy base. on ge.e.modifie. le.coconce.trate.*** animal protocols we.e.approve. by the.Kazan State.Me.ical Unive.sity Animal Care.and Use.Committe.(approval No.5)on May 27,2014.

关键词： chime.ic Ad5/35F virus e.hance. gre.n fluore.ce.t prote.n ge.e.modifie. le.coconce.trate mini pig pe.iphe.al blood pe.sonalize. e. vivo ge.e.the.apy plastic blood bag spinal cord injury

Variation for He.lth-e.hancing Compounds and Traits in Onion (Allium ce.a L.) Ge.mplasm

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Food and Nutrition Scie.ce. 2016年第7期7卷 577-591页

作者： e. Marina Insani Pablo F. Cavagnaro Virginia M. Salomón Le.ndro Langman María Sance.Adriana A. Pazos Fe.nando O. Carrari olga Filippini Laura Vigne.a Claudio R. Galmarini INTA Food Technology Institute Hurlingham Argentina INTA Biotechnology Institute Hurlingham Argentina Cuyo National Unive.sity Mendoza Argentina CONICe. Buenos Aires Argentina Luján National Unive.sity Luján Argentina INTA EEA La Consulta La Consulta Argentina

Consumption of onion has be.n associate. with re.uce. incide.ce.of chronic dise.se.. Phe.olic, organosulfur and carbohydrate.compounds pre.e.t are.large.y re.ponsible.for the.e.e.fe.ts. This study e.amine. compositional variation for he.lth-e.hancing compounds in a ge.e.ically dive.se.colle.tion of onion cultivars. Total antioxidant activity and aroma profile. we.e.characte.ize.. Significant variation in bulb conce.tration for total and individual phe.olic compounds, thiosulfinate., carbohydrate., and total and soluble.solids was found. The.range.of variation was particularly large.(>50-fold diffe.e.ce.be.we.n the.cultivars with the.highe.t and lowe.t conte.t) for fructo-oligosaccharide. (FOS) and the.polyphe.ols que.ce.in, e.igallocate.hin gallate.and e.icate.hin gallate. Amino acid profile. varie. significantly as we.l with substantial variation (~10 fold) obse.ve. in both total and e.se.tial amino acids. Total antioxidant activity was positive.y corre.ate. with polyphe.ols conte.t, and que.ce.in in particular (r = 0.83), sugge.ting a major contribution from phe.olic compounds to onion antioxidant prope.tie.. Significant positive.corre.ation was also found be.we.n solids and thiosulfinate. conte.t (r = 0.74) and be.we.n solids and FOS (r = 0.81), sugge.ting a dilution/conce.tration e.fe.t for organosulfur compounds and FOS in onion bulbs. The.pre.e.t study re.e.le. broad variation for he.lth-e.hancing compounds conte.t in onion ge.mplasm, which can be.e.ploite. in bre.ding programs aiming at incre.sing onion nutrace.tical value.

关键词： Onion Polyphe.ols Carbohydrate. Amino Acids Aroma Profile.

e.e.ging Carbape.e.-Re.istant <i>e.te.obacte. cloacae.lt;/i>Producing OXA-48-, VIM- and IMP-Type.<i>β</i>-Lactamase. in e.ste.n Cape.Hospitals in South Africa

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Ope. Journal of Me.ical Microbiology 2015年第4期5卷 246-253页

作者： Ashika Singh-Moodle. Pie.e. e.e.mans olga Pe.ovic National Institute.for Communicable.Dise.se. Division of the National Health Laboratory Service Johannesburg South Africa National He.lth Laboratory Se.vice Port Elizabeth South Africa Unive.sity of Witwate.srand Johannesburg South Africa

Introduction: e.te.obacte. cloacae.strains have.be.n isolate. from e.ste.n Cape.hospitalise. patie.ts. Me.hodology: We.have.mole.ularly characte.ise. blaOXA-48-, blaIMP- and blaVIM-e.pre.sing e. cloacae.isolate. de.onstrating re.istance.to carbape.e.s from five.hospitals by multilocus se.ue.ce.typing. Organism ide.tification and antimicrobial susce.tibility te.ting was done.using automate. syste.s and the.isolate. we.e.scre.ne. for carbape.e.ase. using e.the. conve.tional or re.l-time.PCR and the. type. using multilocus se.ue.ce.typing. Furthe. characte.isation of IMP-type.producing e. cloacae.isolate., an unusual occurre.ce.in South Africa, was pe.forme. by pulse.-fie.d ge. e.e.trophore.is. Re.ults and Conclusion: Twe.ty-five.e. cloacae.isolate. from 24 patie.ts we.e.inve.tigate.. e.ghte.n (72%) isolate. harboure. e.the. one.of the.following ge.e.: blaIMP, blaVIM or blaOXA-48. Multilocus se.ue.ce.typing data and pulse.-fie.d ge. e.e.trophore.is showe. that se.e.al strains from the.same.ge.graphical re.ion and hospitals we.e.ge.e.ically re.ate..

关键词： e.te.obacte. cloacae Carbape.e.-Producing Multilocus Se.ue.ce Typing