Emerging Carbapenem-Resistant <i>Enterobacter cloacae</i>Producing OXA-48-, VIM- and IMP-Type-<i>β</i>-Lactamases in Eastern Cape Hospitals in South Africa

作     者：Ashika Singh-Moodley Pieter Ekermans Olga Perovic 

作者机构：National Institute for Communicable Diseases Division of the National Health Laboratory Service Johannesburg South Africa National Health Laboratory Service Port Elizabeth South Africa University of Witwatersrand Johannesburg South Africa 

出 版 物：《Open Journal of Medical Microbiology》 (医学微生物学（英文）)

年 卷 期：2015年第5卷第4期

页      面：246-253页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Enterobacter cloacae Carbapenem-Producing Multilocus Sequence Typing 

摘      要：Introduction: Enterobacter cloacae strains have been isolated from Eastern Cape hospitalised patients. Methodology: We have molecularly characterised blaOXA-48-, blaIMP- and blaVIM-expressing E. cloacae isolates demonstrating resistance to carbapenems from five hospitals by multilocus sequence typing. Organism identification and antimicrobial susceptibility testing was done using automated systems and the isolates were screened for carbapenemases using either conventional or real-time PCR and then typed using multilocus sequence typing. Further characterisation of IMP-type-producing E. cloacae isolates, an unusual occurrence in South Africa, was performed by pulsed-field gel electrophoresis. Results and Conclusion: Twenty-five E. cloacae isolates from 24 patients were investigated. Eighteen (72%) isolates harboured either one of the following genes: blaIMP, blaVIM or blaOXA-48. Multilocus sequence typing data and pulsed-field gel electrophoresis showed that several strains from the same geographical region and hospitals were genetically related.