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Performance of low temperature Microbial Fuel Cells(MFCs)catalyzed by mixed bacterial consortia

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Journal of Environmental Sciences 2017年第2期29卷 284-292页

作者： olga Tkach Thangavel Sangeetha Spiridonova Maria Aijie Wang State Key Laboratory of Urban Water Resources and Enuironment(SKLUWRE) Harbin Institute of TechnologyHarbin 150090China. Key Laboratory of Environmental biotechnology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Krasnoyarsk State Institution of Railway Vehicles Krasnoyarsk 660028Russia

Microbial Fuel Cells（MFCs） are a promising technology for treating wastewater in a sustainable manner. In potential applications, low temperatures substantially reduce MFC performance. To better understand the effect of temperature and particularly how bioanodes respond to changes in temperature, we investigated the current generation of mixed-culture and pure-culture MFCs at two low temperatures, 10°C and 5°C. The results implied that the mixed-culture MFC sustainably performed better than the pure-culture（Shewanella） MFC at 10°C, but the electrogenic activity of anodic bacteria was substantially reduced at the lower temperature of 5°C. At 10°C, the maximum output voltage generated with the mixed-culture was 540–560 m V, which was 10%–15% higher than that of Shewanella MFCs. The maximum power density reached 465.3 ± 5.8 m W/m^2 for the mixed-culture at10°C, while only 68.7 ± 3.7 m W/m^2 was achieved with the pure-culture. It was shown that the anodic biofilm of the mixed-culture MFC had a lower overpotential and resistance than the pure-culture MFC. Phylogenetic analysis disclosed the prevalence of Geobacter and Pseudomonas rather than Shewanella in the mixed-culture anodic biofilm, which mitigated the increase of resistance or overpotential at low temperatures.

关键词： Microbial fuel cell Psychrophilic bacteria Shewanella Low temperature bioelectrochemical

Mate choice is affected by parasite infestation rate of the choosing individual as well as of potential mating partners

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Current Zoology 2023年第5期69卷 559-567页

作者： Alejandro Cantarero olga VDolnik Matteo Griggio Herbert Hoi Department of Physiology Veterinary SchoolComplutense University of MadridAvenida Puerta de Hierro s/n28040 MadridSpain Department of Integrative biology and Evolution Konrad Lorenz Institute of Ethology(KLIVV)University of Veterinary Medicine ViennaSavoyenstraße 1aA-1160 ViennaAustria Dipartimento di biologia Universitàdi PadovaVia U.Bassi 58/BI-35131 PadovaItaly

Parasites are known to be a key driving force in mate choice and are important for the expression and evolution of ornaments and behavioral traits being ***,there is little experimental evidence on how the parasite’s burden of the choosing individual is integrated into the mate-choice process and how it affects decision-making,especially in relation to parasite infestation of potential ***,the aim of our study was to determine whether female house sparrows Passer domesticus adjust their mate preference according to their own as well as the parasite load of prospective *** do this,we experimentally manipulated female parasite load and determined their mate preferences prior to and after parasite *** manipulated the chronic coccidian parasite burden of females either by initiating the acute infection phase via re-infecting them with coccidian or by temporally reducing the parasite load of *** then measured the effect of this manipulation on mate preference by presenting females with a choice of four stimuli:three males with similar ornaments,but unmanipulated,naturally varying chronic coccidiosis levels,and an unmanipulated control ***,we recorded some males’behavior in relation to their infection status pointing toward an increased or reduced interest in *** found that females preferred highly infested males prior to manipulation,regardless of their own infestation ***,after manipulation,infested females avoided highly infested males probably in response to the deterioration of their health condition by *** study suggests that mate-choice decisions are more complex when they are mediated by *** implications of parasites for evolutionary theories of sexual signaling and mate choice are discussed.

关键词： coccidiosis female decision mate choice parasites Passer domesticus Isospora spp

The whole day matters: Understanding 24-hour movement guideline adherence and relationships with health indicators across the lifespan

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Journal of Sport and Health Science 2020年第6期9卷 493-510页

作者： Scott Rollo olga Antsygina Mark S.Tremblay Healthy Active Living and Obesity Research Group Children’s Hospital of Eastern Ontario Research InstituteOttawaONK1H 8L1Canada Department of Pediatrics Faculty of MedicineUniversity of OttawaOttawaONK1H 8M5Canada Department of Health Sciences Carleton UniversityOttawaONK1S 5B6Canada

background:New research suggests that the composition(mix)of movement behaviors within a 24-h period may have important implications for health across the *** with this integrated movement behavior paradigm,a number of countries across the world have developed and released 24-h movement guidelines for specific age *** purpose of this systematic review was to examine the associations between the 24-h time-use composition of movement behaviors,or adherence to 24-h movement guidelines,and multiple health indicators across the ***:Five online databases(PsycINFO,PubMed,SPORTDiscus,Web of Science,and Ovid MEDLINE)were searched for relevant peer-reviewed studies published between January 2015 and January 2020 that met the a priori inclusion criteria,with no study design *** methodological quality of research evidence for each individual study and for each health indicator was assessed by using a modified version of the Downs and black ***:A total of 51 studies from 20 different countries met the inclusion criteria.A total of 31 studies examined adherence(meeting *** meeting)to 24-h movement guidelines,and 20 studies used compositional analyses to explore the 24-h time-use composition of movement *** indicated that meeting the 24-h movement guidelines were(1)not associated with adiposity among toddlers,(2)favorably associated with health-related quality of life,social-cognitive development,and behavioral and emotional problems among preschoolers,(3)favorably associated with global cognition,health-related quality of life,and healthy dietary patterns in children,and(4)favorably associated with adiposity,fitness,and cardiometabolic,mental,social,and emotional health among children and *** associations were also found between the composition of 24-h movement behaviors and indicators of(1)adiposity and bone and skeletal health among preschoolers,(2)health-related quality of life among child

关键词： Compositional analysis Movement behaviors Physical activity Sedentary behavior Sleep

Efficiency of high-loaded nickel catalysts modified by Mg in hydrogen storage/extraction using quinoline/decahydroquinoline pair as LOHC substrates

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Journal of Magnesium and Alloys 2024年第8期12卷 3245-3263页

作者： Anton P.Koskin Jingsong Zhang olga b.belskaya olga A.bulavchenko Darya A.Konovalova Sergey A.Stepanenko Arkadiy V.Ishchenko Irina G.Danilova Vyacheslav L.Yurpalov Yurii V.Larichev Roman G.Kukushkin Petr M.Yeletsky Federal Research Center boreskov Institute of Catalysis Sb RAS Acad.Lavrentiev av.5Novosibirsk630090Russian Federation Center of New Chemical Technologies bIC Boreskov Institute of CatalysisNeftezavodskaya st.54Omsk 644040Russia

An effect of Mg introduction on efficiency of high-loaded nickel catalysts in dehydrogenation of decahydroquinoline(10HQ)was inves-tigated.10HQ dehydrogenation is key process for the liquid organic hydrogen carrier(LOHC)storage technology using the quinoline/10HQ pair as H_(2)-lean/H_(2)-rich *** influence of synthesis technique of Ni/Mg/Al catalysts on their properties has been *** catalysts were synthesized through coprecipitation of Ni,Mg,Al precursors to obtain layered double hydroxides(LDH)or via syn-thesis of(∼72 wt%)Ni-Al_(2)O_(3) system-also through coprecipitation,followed by modifying with a magnesium-containing *** the catalysts of the first series,the inclusion of magnesium into LDH lattice led to a significant increase in catalytic activity in hydrogen extraction(10HQ dehydrogenation reaction).Despite the decrease in the content of catalytically active nickel,a significant increase in the yield of the dehydrogenation product was *** regularity is presumably associated with appearance of basic sites,that accelerates the dehydrogenation *** the case of the second series,activity of pre-reduced(600°C,H_(2))catalysts in dehydrogenation of 10HQ also significantly depends on a MgO content and is maximal at Mg:Ni weight ratio *** an in-depth study of structure of the original and reduced catalyst samples(Ni-Al_(2)O_(3) and Ni-MgNiOx-Al_(2)O_(3)),it was shown that this regularity is associated with the increased resistance of catalytically active Ni particles to agglomeration during the reductive ***,using the Ni-MgNiOx-Al_(2)O_(3)catalyst for hydrogen storage process(hydrogenation reaction),the possibility of deep quinoline hydrogenation(up to 10HQ)in a flow-type reactor was demonstrated for the first time.

关键词： Mg-modified catalysts High-loaded nickel catalyst Layered double hydroxides Hydrogen storage LOHC Dehydrogenation

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薄荷油纳米乳液消化规律与稳定性研究

农业机械学报 2018年第10期49卷 381-386,426页

作者：王中江张潇元车佳玲李杨 olga O b 江连洲东北农业大学食品学院哈尔滨150030 克麦罗沃国立大学食品学科与技术学院克麦罗沃650056

研究了薄荷油纳米乳液在体外模拟消化过程中乳液包埋对其平均粒径、ζ-电位、游离脂肪酸释放率以及薄荷醇生物可利用度的影响。激光扫描3D共聚焦显微镜观察薄荷油纳米乳液的显微结构,研究发现薄荷油完全被SPI包埋,纳米乳液液滴呈球状形... 详细信息

研究了薄荷油纳米乳液在体外模拟消化过程中乳液包埋对其平均粒径、ζ-电位、游离脂肪酸释放率以及薄荷醇生物可利用度的影响。激光扫描3D共聚焦显微镜观察薄荷油纳米乳液的显微结构,研究发现薄荷油完全被SPI包埋,纳米乳液液滴呈球状形态,表明大豆蛋白完整地吸附于纳米乳液的油-水界面处,呈现出核壳状结构。高压均质处理制备的薄荷油纳米乳液的游离脂肪酸释放率及薄荷醇生物可利用度远大于薄荷油的对照组。薄荷油纳米乳液在模拟胃消化阶段,乳液的平均粒径、ζ-电位均变大,乳液的微观结构表明消化体系出现液滴聚合现象;在模拟肠液消化后,薄荷油纳米乳液的界面蛋白被水解,油滴被消化,乳液的平均粒径减小、ζ-电位绝对值增加。通过多重光散射稳定性分析仪检测薄荷油纳米乳液的稳定性,薄荷油稳定性指数为2.8,且检测过程中并未出现乳液上浮和絮凝等现象。

关键词：薄荷油纳米乳液高压均质生物可利用度消化规律稳定性

High-performance cascade nanoreactor based on halloysite nanotubes-integrated enzyme-nanozyme microsystem

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Chinese Chemical Letters 2022年第2期33卷 807-811页

作者： Yan Liu Rui Lv Shiyong Sun Daoyong Tan Faqin Dong Yevgeny A.Golubev Xiaoqin Nie olga b.Kotova Jin Liu Ke Wang School of Environment and Resource Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of EducationSouthwest University of Science and TechnologyMianyang 621010China Yushkin’s Institute of Geology Komi Science CenterUral Branch of RASul.Pervomayskaya54Syktyvkar 167982Russia

Various enzymatic reactions or enzymatic cascade reactions occur efficiently in biological microsystems due to space constraints or orderly transfer of intermediate products. Inspired by this, the horseradish peroxidase(HRP)-like nanozyme(Fe-aminoclay) was in situ synthesized on the surface of alkali-activated halloysite nanotubes and the natural enzyme(glucose oxidase, GOx) was immobilized on it to construct a high-efficiency GOx-Fe AC@AHNTs cascade nanoreactor. In which, Fe AC@AHNTs can not only be used as a carrier for immobilized enzymes, but also help its catalytic activity to cooperate with glucose oxidase in a cascade reaction. The microcompartments and substrate channel effect of this enzyme-nanozyme microsystem exhibit a superior catalytic performance than that of natural enzyme system, and exhibits excellent long-term stability and recyclability. Subsequently, the GOx-Fe AC@AHNTs cascade nanoreactor was employed as a glucose colorimetric platform, which displayed a low detection limit(0.47 μmol/L)in glucose detection. This enzyme-nanoenzyme nanoreactor provides a simple and effective example for constructing a multi-enzyme system with limited space, and lays the foundation for subsequent research in the fields of biological analysis and catalysis.

关键词： Halloysite nanotubes Fe-aminoclay Nanozyme Cascade nanoreactor Catalysis enhancement

Interactions of primary neuroepithelial progenitor and brain endothelial cells: distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact

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Cell Research 2007年第7期17卷 619-626页

作者： Miguel A Gama Sosa Rita De Gasperi Anne b Rocher Gissel M perez Keila Simons Daniel E Cruz Patrick R Hof Gregory A Elder Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA Neuroscience Mount Sinai School of Medicine of New York University New York NY Department of Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA

Neurovascular interactions are crucial for the normal development of the central nervous system. To study suchinteractions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelialcell fractions from embryonic mouse brains. Depending on the culture conditions endothelial cells were found to favormaintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differ-entiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellularinteractions needed for the proper development of the brain.

关键词： differentiation culture endothelial cells neuroepithelial cells

Potentiality of Vermicompost Humic Acids in banana in Vitro Micropropagation Clone： Enano Guantanamero

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Journal of Environmental Science and Engineering(A) 2013年第11期2卷 677-685页

作者： Marcia b. Moya Fernandez Luis Eduardo Cosio-Vargas Daniel Cabezas Montero Andres Calderin Garcia Dany Marrero Lopez Sandra perez alvarez Deperment of Plant Protection Agrarian University of Havana (Fructuoso Rodriguez Perez) Mayabeque 32 700 Cuba INIFAP (Nacional Research Center of Forest Agrarian and Livestock) Uruapan 60150 Mexico Havana biofabric Mayabeque 32700 Cuba

The research was done at Havana biofabric, Mayabeque Province. The vitro plants of banana （Musa sp） used came from the clone Enano Guantanamero （viand type）. They come from multiplication phase in a Murashige and MS （Skoog medium）. The objective of this work was to study the potentialities of HA （humic acids） for stimulation of rooting and cell division process, searching for completely synthetic hormone substitution due to its high cost. The multiplication medium contained thiamine （2 mg·L^-1）, myo-inositol （100 mg·L^-1）, sugar （30mg·L^-1）. by the use of HA, five treatments were used with total substitution of auxin and cytokinin （TI： HA （10 ***）; （T2： HA （20 mg·L^-1）; （T3： HA （30mg·L^-1）; （T4： HA （40 mg·L^-1）; （T5： HA （50 mg·L^-1） and the control with 6bAP （benzil amino purine）（4 mg·L^-1）＋ IbA （indol butyric acid）（0.65 mg·L^-1）, pH of the medium was adjust just before agar were add. The results showed that in the explants under HA treatments, elongation and cell multiplication were favored confirmed by explants great high, root number and length due to dry mass. The total protein content, reduced carbohydrate and peroxidases enzymatic activity were determine. The use of Vermicompost HA in the in vitro micropropagation of banana allow the elimination of rooting phase saving materials and be able to pass explants directly to Acclimatization phase.

关键词： banana multiplication phase HA (Humic Acids) rooting.

In vivo assessment of structural changes of the urethra in lower urinary tract disease using cross-polarization optical coherence tomography

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Journal of Innovative Optical Health Sciences 2020年第6期3卷 55-70页

作者： Elena b.Kiseleva Alexander A.Moiseev Anton S.Kuyarov Muhammat A.Molvi Grigory V.Gelikonov Anna V.Maslennikova olga S.Streltsova Privolzhsky Research Medical University 10/1 Minin and Pozharsky Square 603005 Nizhny NovgorodRussia Institute of Applied Physics Russian Academy of Sciences Ulyanova Street 46603950Nizhny NovgorodRussia

The paper presents the results of a study of the female urethra in cases of urethral pain syndrome(UPS)and inflammatory diseases of the lower urinary tract using cross-polarization optical coherence tomography(CP OCT).Urethral wall structure was studied in 86 patients;233 CP OCT images were collected.A comparative qualitative analysis of three groups of CP OCT images—"norm","Inflammation"and"UPS"—identified that despite the absence of a clear inflammatory factor in the patient's examination,the urethral tissues in UPS were in an altered *** changes in the urethral wall with UPS and in cases of inflammation were *** a point scale,three experts independently performed visual scoring of the CP OCT *** parameters:epithelial contrast,cavities and the minimum signal depth in the co-channel were *** was found that,individually,the parameters correlate only weakly with the *** under the receiver operating characteristic(ROC)curve was from 0.51 to *** joint use of a number of visual signs has a greater diagnostic value than the use of the criteria *** under the ROC curve using the developed cumulative criterion reached up to *** study could form the basis of a scoring system for assessing the state of the urethral tract using CP OCT images in real *** CP OCT method provides information on the state of urethral tissues that cannot be obtained with traditional cystoscopy.

关键词： Cross-polarization optical coherence tomography urethral pain syndrome connective tissue visual analysis of images automatic classification of images