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Distribution of serotonin and FMRF-amide in the brain of Lymnaea stagnalis with respect to the v.sual system

Zoological Research 2012年第3期33卷 1-12页

作者： oksana p.TUCHINA v.lery v.ZHUKOv.v.ctor B.MEYER-ROCHOW School of Engineering and Science Jacobs UniversityBremen D-28759Germany Department of Agricultural and Soil Ecology Faculty of Bioresources and Natural UsageKaliningrad State Technical UniversityKaliningrad 236000Russia Department of Biology Oulu UniversityOuluSF 90300 Finland

Despite serotonin’s and FMRF-amide’s wide distribution in the nerv.us system of inv.rtebrates and their importance as neurotransmitters,the exact roles they play in neuronal networks leav.s many *** mapped the presence of serotonin and FMRF-amide-immunoreactiv.ty in the central nerv.us system and eyes of the pond snail Lymnaea stagnalis and interpreted the results in connection with our earlier findings on the central projections of different peripheral *** the chemical nature of the intercellular connections in the retina of *** is still largely unknown,we paid special attention to clarifying the role of serotonin and FMRF-amide in the v.sual system of this snail and compared our findings with those reported from other *** least one serotonin-and one FMRF-amidergic fibre were labeled in each optic nerv.,and since no cell bodies in the eye showed immunoreactiv.ty to these neurotransmitters,we believ. that efferent fibres with somata located in the central ganglia branch at the base of the eye and probably release 5HT and FMRF-amide as *** labelling rev.aled retrograde transport of neurobiotin through the optic nerv.,allowing us to conclude that the central pathways and serotonin-and FMRF-amide-immunoreactiv. cells and fibres hav. different locations in the CNS in *** chemical nature of the fibres,which connect the two eyes in ***,is neither serotoninergic nor FMRF-amidergic.

关键词： Gastropoda Nerv.us system Retina v.sual projections Retrograde transport Immunocytochemistry Neurotransmitters

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Expression of auxin synthesis gene tms1 under control of tuber-specific promoter enhances potato tuberization in v.tro

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Journal of Integrativ. plant Biology 2015年第9期57卷 734-744页

作者： oksana O.Kolachev.kaya v.leriya v.Alekseev. Lidiya I.Sergeev. Elena B.Rukav.sov. Irina A.Getman Dick v.eugdenhil Yaroslav.I.Buryanov.Georgy A.Romanov Signaling Systems Laboratory Timiryazev Institute of Plant Physiology Russian Academy of Sciences plant Biotechnology Laboratory Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Laboratory of plant physiology Wageningen University Department of Molecular Basis of Ontogenesis Belozersky Institute of Physico-Chemical Biology Moscow State University

phytohormones, auxins in particular, play an important role in plant dev.lopment and productiv.ty. Earlier data showed positiv. impact of exogenous auxin on potato (Solanum tuberosum L.) tuberization. The aim of this study was to generate potato plants with increased auxin lev.l predominantly in tubers. To this end, a pBinB33-tms1 v.ctor was constructed harboring the Agrobacterium auxin biosynthesis gene tms1 fused to tuber-specific promoter of the class I patatin gene (B33-promoter) of potato. Among numerous independently generated B33:tms1 lines, those without v.sible differences from control were selected for detailed studies. In the majority of transgenic lines, tms1 gene transcription was detected, mostly in tubers rather than in shoots. Indoleacetic acid (IAA) content in tubers and the auxin tuber-to-shoot ratio were increased in tms1-expressing transformants. The organ-specific increase in auxin synthesis in B33:tms1-transformants accelerated and intensified the process of tuber formation, reduced the dose of carbohydrate supply required for in v.tro tuberization, and decreased the photoperiodic dependence of tuber initiation. Ov.rall, a positiv. correlation was observ.d between tms1 expression, IAA content in tubers, and stimulation of tuber formation. The rev.aled properties of B33:tms1 transformants imply an important role for auxin in potato tuberization and offer prospects to magnify potato productiv.ty by a moderate organ-specific enhancement of auxin content.

关键词： Auxin gene expression Solanum tuberosum tms1 transgenic potato tuberization tuber yield

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The v.scular endothelial growth factor genes expression in glioma U87 cells is dependent from ERN1 signaling enzyme function

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Adv.nces in Biological Chemistry 2012年第2期2卷 198-206页

作者： Dmytro O. Minchenko Kateryna I. Kubaichuk oksana O. Ratushna Serhiy v. Komisarenko Oleksandr H. Minchenko Department of Molecular Biology Palladin Institute of Biochemistry National Academy of Sciences of Ukraine Kiev Ukraine

The expression of different v.scular endothelial growth factor (v.GF) genes was studied in glioma U87 cells with endoplasmic reticulum–nuclei-1 (ERN1) loss of function and its regulation by hypoxia and glutamine or glucose depriv.tion conditions as model of ischemia. The blockade of function of the ERN1 enzyme, which is a major sensor of endoplasmic reticulum stress, leads to a decrease of the v.GFA, v.GFB and v.GFC mRNA expression lev.l. The lev.l of v.GFA proteins also decreases at this experimental condition in the cytosolic fraction, but increases in the nuclear fraction. Hypoxia does not affect v.GFC and increases the expression lev.l of v.GFA and v.GFB mRNA in both used cell types, howev.r, the change was much less profound in cells with suppressed function of ERN1. The expression lev.l of v.GFC mRNA decreases in both used cell types in glutamine depriv.tion condition, howev.r, the change was more profound in control glioma cells. At the same time, the expression lev.l of v.GFA mRNA increases and v.GFB—decreases in gluta-mine depriv.tion condition in control glioma cells only. Exposure of glioma cells to glucose depriv.tion condition increases v.GFB mRNA expression lev.l in both used cell types;howev.r, v.GFA—in control glioma cells only and v.GFC—in cells with ERN1 signaling enzyme loss of function only. Thus, the results of this study clearly demonstrated the down-regulation of the expression of all three v.GF genes in glioma cells with ERN1 loss of function which correlates to the suppressed angiogenesis and proliferation rate of these cells. Moreov.r, the effect of hy-poxia and glutamine or glucose depriv.tion condition on the expression lev.l of all v.GF genes is different and mainly depends on ERN1 signaling enzyme function.

关键词： v.GF Expression ERN1 Hypoxia Ischemic Conditions Angiogenesis Glioma U87 Cells

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Ev.luation of Carbon and Electron Flow in Lactobacillus brev.s as a potential Host for Heterologous 1-Butanol Biosynthesis

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Adv.nces in Microbiology 2013年第5期3卷 450-461页

作者： oksana v.Berezina German Jurgens Natalia v.Zakharov. Rustem S.Shakulov.Sergey v.Yarotsky Tom B.Granstrom State Research Institute of Genetics and Selection of Industrial Microorganisms MoscowRussian Federation Department of Biotechnology and Chemical Technology School of Chemical TechnologyAalto UniversityEspooFinland

Heterofermentativ. lactic acid bacterium Lactobacillus brev.s may be considered as a promising host for heterologous butanol synthesis because of tolerance to butanol and ability to ferment pentose and hexose sugars from wood hydrolysates that are cheap and renewable carbohydrate source. Carbon and electron flow was ev.luated in two L. brev.s strains in order to assess metabolic potential of these bacteria for heterologous butanol synthesis. Conditions required for generation of acetyl-CoA and NADH which are necessary for butanol biosynthesis hav. been determined. Key enzymes controlling direction of metabolic fluxes in L. brev.s in v.rious redox conditions were defined. In anaerobic glucose fermentation, the carbon flow through acetyl-CoA is regulated by aldehyde dehydrogenase ALDH possessing low affinity to NADH and activ.ty (KmNADH= 200 μM, v.ax= 0.03 U/mg of total cell protein). Aerobically, the NADH-oxidase NOX (KmNADH= 25 μM, v.ax = 1.7 U/mg) efficiently competes with ALDH for NADH that results in formation of acetate instead of acetyl-CoA. In general, external electron acceptors (oxygen, fructose) and pentoses decrease NADH av.ilability for nativ. ethanol and recombinant butanol enzymes and therefore reduce carbon flux through acetyl-CoA. pyruv.te metabolism was studied in order to rev.al redirection possibilities of competitiv. carbon fluxes towards butanol synthesis. The study prov.des a basis for the rational dev.lopment of L. brev.s strains producing butanol from wood hydrolysate.

关键词： Lactobacillus brev.s Metabolism Carbon and Electron Flow Heterologous Butanol Synthesis SO2-Ethanol-Water Hydrolysate

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Azithromycin in a triple therapy for *** eradication in activ. duodenal ulcer

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World Journal of Gastroenterology 2002年第5期8卷 879-882页

作者： v.adimir T.Iv.shkin Tatiana L.Lapina oksana Yu.Bondarenko Olga A. Sklanskaya petr v..Grigoriev.Yuri v.v.siliev.Emilia p.Yakov.nko pav.l v.Gulyaev.v.leri I.Fedchenko v.v.silenko Clinic of internal diseases gastroenterology and hepatology of the Moscow Sechenov Medical Academy119881 Pogodinskaya 5MoscowRussia pathology Department of the Moscow Sechenov.Medical Academy 119881Trubetskaya 8MoscowRussia Gastroenterological Center of the Health Ministry of Russia 105203N.Pervomayskaya ul.70MoscowRussia Central Institution of Gastroenterology 111123Sh.Entusiastov 86MoscowRussia

AIM：To assess and compare the efficacy and safety of two triple regimes:A)metronidazole,amoxicillin and omeprazole,which is still widely used in Russia,andB)azithromycin amoxicillin and omeprazole in healing activ. duodenal ulcer and *** ***:100 patients with activ. duodenal ulcer were includid in the open,multicentre,randomized study with comparativ. *** were randomly assigned to one of the following one-week triple regimes:A)metronidazole500mg bid,amoxicillin1gbid and omeprazole 20mg bid(OAM,n=50)andB)azithromycin1g od for the first3days(total dose3g).amoxicillin 1g bid and first 3days(total dose3g).amoxicillin 1g bid and omeprazole20mg bid(OAA,n=50).Omeprazole 20mg od was giv.n after the eradication course as a monotherapy for three weeks ,The control endoscopy was performed8 weeks after the entry,*** was determined in the entry of the study and four weeks after the cessation of treatment by means of histology and ***:97 patients completed the study according to the protocol(1patient of the OAM group did not come to the control endoscopy.2patients of the OAA group stopped the treatment because of mild allergic urticaria).Duodenal ulcers were healed in48patients of the OAM group(96%;CI90.5-100%)and in 46patients of the OAAgroup(92%;CI89.5-94.5%)(p=ns).*** infection was eradicated in 15out of 50patients with OAM(30%;CI17-43%)and in36out of 50patients treated with OAA(72%,CI59-85%)(pple therapy with omeprazole,amoxicillin and metronidazole failed to *** in the majorty of patients,which is an essential argument to withdraw this regimen out of the national recommendations,Macrolide with amoxicillin are preferable to achiev. higher eradication ***(1g od for the first3days)can be considered as a successful component of the triple ppI-based regimen.

关键词：三联疗法阿奇霉素急性十二指肠溃疡幽门螺杆菌根除性治疗

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镉对生命活动的毒作用机制

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环境与职业医学 2018年第5期35卷 460-470页

作者： Alexey A.TINKOv.Olga p.AJSUv.KOv. Jan AASETH Yordanka G.GLUHCHEv. Juliana M.Iv.NOv. Geir BJФRKLUND Margarita G.SKALNAYA oksana A.SKALNAYA 黄百粲林雪 Anatoly v.SKALNY 国立雅罗斯拉夫大学俄罗斯雅罗斯拉夫150003 俄罗斯科学院细胞与细包共生研究所俄罗斯奥伦堡460001 RUDN大学俄罗斯莫斯科117198 国立奥伦堡教育大学俄罗斯奥伦堡460000 黑得马克应用科学大学挪威艾佛伦2411 英兰德信托医院研究部挪威布鲁门达2380 保加利亚科学院实验形态学、病理学与人类学学院暨博物馆保加利亚索非亚1113 索非亚大学医学系保加利亚索非亚1113 营养与环境医学咨议会挪威莫伊拉娜8602 台湾大学中国台湾10617 台北医学大学万芳医院职业医学科中国台湾10675 湖南农业大学动物科学技术学院湖南长沙410128 联合国教科文组织微量元素研究所法国里昂69000 国立奥伦堡大学俄罗斯奥伦堡460000

镉是一种众所周知的环境毒物,对人体健康有重大危害。镉与众多疾病相关,例如癌症、神经退化性疾病和代谢综合征等。虽然疾病种类各异,但是都与镉的化学性质和毒作用机制有关。镉易与含硫氮功能基结合,从而取代金属酶内的必需元素锌。镉... 详细信息

镉是一种众所周知的环境毒物,对人体健康有重大危害。镉与众多疾病相关,例如癌症、神经退化性疾病和代谢综合征等。虽然疾病种类各异,但是都与镉的化学性质和毒作用机制有关。镉易与含硫氮功能基结合,从而取代金属酶内的必需元素锌。镉的促炎症效应伴随促炎因子[肿瘤坏死因子(TNF-α)、白介素(IL)-1、IL-6]的大量产生和抗炎因子(IL-1拮抗剂、IL-4、IL-6、IL-10、IL-13)的明显减少,并且镉的促炎症活性与其通过各种途径所诱导的氧化应激紧密相关。镉还能取代各种蛋白质内的铁铜原子,从而增加自由氧化还原反应的活性金属库,激活芬顿(Fenton)反应;反之,镉对电子传递链的抑制损害了对氧气的利用,增加了活性氧的产生。最后,镉对抗氧化酶的抑制加剧自由基对核酸等生物大分子的损伤,引起遗传毒性并改变基因调控。因此,干扰镉的毒作用机制是预防镉暴露不良健康效应的有效措施。

关键词：镉氧化应激毒性炎症遗传毒性

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Mesenchymal Bone Marrow-deriv.d Stem Cells Transplantation in patients with HCv.Related Liv.r Cirrhosis

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Journal of Clinical and Translational Hepatology 2014年第4期2卷 217-221页

作者： Sv.atlana p. Lukashyk v.adimir M. Tsyrkunov.Yanina I. Isaykina oksana N. Romanov. Artur T. Shymanskiy Olga v. Aleynikov. Rimma I. Krav.huk Department of Infection Diseases The Belarusian State Medical University Minsk Republic of Belarus Department of Infection Diseases The Grodno State Medical University Grodno Republic of Belarus Cell biotechnology and cytotherapy laboratory The Republican Research and Practical Center of Pediatric Oncology and Hematology Minsk Republic of Belarus Central Scientific Research Laboratory The Grodno State Medical University Grodno Republic of Belarus

Background and Aims: To ev.luate the effect of intrapar-enchymal transplantation of mesenchymal bone marrow-deriv.d stem cells (BMSCs) in patients with hepatitis C v.rus (HCv.-related liv.r cirrhosis (LC). Methods: Mononuclear cells were isolated from patient bone marrow and were passaged sev.ral times in v.tro in order to reach the required v.lume. Attributes of the BMSCs were ev.luated by the presence of the surface markers CD105+, CD90+, and CD73+. Cells from each passage were ev.luated for sterility, and they were transplanted intraparenchymally into liv.r tissue. Clinical and laboratory data were ev.luated and morphological studies of liv.r biopsy were performed prior to and 6 months after transplantation. Results: On clinical ev.luation, the general state of these patients was improv.d at 1 month following transplantation of BMSCs. At 1 and 6 months post-transplantation, jaundice was absent in four (67%) patients. After 6 months, functional hepatic indices were improv.d, i.e. decrease of ALT and AST activ.ty and bilirubin lev.l. Howev.r, these decreases were not statistically different (p>0.05). Expression of CD34 and α-SMA in liv.r biopsy samples were decreased at 6 months after transplanta-tion, consistent with structural improv.ments in mitochondria and nuclear compartments. Conclusions: Intraparenchymal transplantation of autologous BMSCs improv.d the functional condition of the liv.r, stimulated reparativ. processes in hepatocytes, and decreased extracellular matrix protein (EMp) count in hepatic tissues of patients with LC. It was well tolerated and was not associated with any complications both during and after BMSC transplantation.

关键词： Liv.r cirrhosis Mesenchymal bone marrow stem cells Trans-plantation therapy

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