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Enhanced osteoarthritis therapy by nanoengineered mesenchymal stem cells using biomimetic CuS nanoparticles loaded with plasmid DNA encoding TGF-β1

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Bioactive Materials 2023年第1期19卷 444-457页

作者： Yu Cai Cuixi Wu Qianhua ou Muhui Zeng Song xue Jieli Chen Yao Lu Changhai Ding Clinical Research Center Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong510282China Orthopedic Center Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong510282China Guangdong Key Lab of Orthopedic Technology and Implant GuangzhouGuangdong510010China Department of Rheumatology and Immunology Arthritis Research InstituteThe First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui230032China Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases Academy of OrthopedicsSouthern Medical UniversityGuangzhouGuangdong510630China Menzies Institute for Medical Research University of TasmaniaHobartTasmania7000Australia

Mesenchymal stem cells(MSCs)therapy shows the potential benefits to relieve clinical symptoms of osteoarthritis(OA),but it is uncertain if it can repair articular cartilage lesions-the main pathology of OA.Here,we prepared biomimetic cupper sulfide@phosphatidylcholine(CuS@PC)nanoparticles(NPs)loaded with plasmid DNA(pDNA)encoding transforming growth factor-beta 1(TGF-β1)to engineer MSCs for enhanced OA therapy via cartilage regeneration.We found that the NPs not only promoted cell proliferation and migration,but also presented a higher pDNA transfection efficiency relative to commercial transfection reagent lipofectamine 3000.The resultant CuS/TGF-β1@PC NP-engineered MSCs(termed CTP-MSCs)were better than pure MSCs in terms of chondrogenic gene expression,glycosaminoglycan deposition and type II collagen formation,favoring cartilage repair.Further,CTP-MSCs inhibited extracellular matrix degradation in interleukin-1β-induced chondrocytes.Consequently,intraarticular administration of CTP-MSCs significantly enhanced the repair of damaged cartilage,whereas pure MSCs exhibited very limited effects on cartilage regeneration in destabilization of the medial meniscus(DMM)surgical instability mice.Hence,this work provides a new strategy to overcome the limitation of current stem cell therapy in OA treatment through developing more effective nanoengineered MSCs.

关键词： Osteoarthritis Stem cell therapy Cartilage regeneration Cupper sulfide nanoparticles Gene delivery

Advances in Noble Metal-Decorated Metal Oxide Nanomaterials for Chemiresistive Gas Sensors:Overview

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纳微快报：英文版 2023年第6期15卷 353-427页

作者： Li‑Yuan Zhu Lang‑Xi ou Li‑Wen Mao xue‑Yan Wu Yi‑Ping Liu Hong‑Liang Lu State Key Laboratory of ASIC and System Shanghai Institute of Intelligent Electronics and SystemsSchool of MicroelectronicsFudan UniversityShanghai 200433People’s Republic of China School of Opto‑Electronic Information and Computer Engineering University of Shanghai for Science and TechnologyShanghai 200093People’s Republic of China State Key Laboratory of Metal Matrix Composites School of Material Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Highly sensitive gas sensors with remarkably low detection limits are attractive for diverse practical application fields including real-time environmental monitoring,exhaled breath diagnosis,and food freshness analysis.Among various chemiresistive sensing materials,noble metal-decorated semiconducting metal oxides(SMOs)have currently aroused extensive attention by virtue of the unique electronic and catalytic properties of noble metals.This review highlights the research progress on the designs and applications of different noble metal-decorated SMOs with diverse nanostructures(e.g.,nanoparticles,nanowires,nanorods,nanosheets,nanoflowers,and microspheres)for high-performance gas sensors with higher response,faster response/recovery speed,lower operating temperature,and ultra-low detection limits.The key topics include Pt,Pd,Au,other noble metals(e.g.,Ag,Ru,and Rh.),and bimetals-decorated SMOs containing ZnO,SnO_(2),WO_(3),other SMOs(e.g.,In_(2)O_(3),Fe_(2)O_(3),and CuO),and heterostructured SMOs.In addition to conventional devices,the innovative applications like photo-assisted room temperature gas sensors and mechanically flexible smart wearable devices are also discussed.Moreover,the relevant mechanisms for the sensing performance improvement caused by noble metal decoration,including the electronic sensitization effect and the chemical sensitization effect,have also been summarized in detail.Finally,major challenges and future perspectives towards noble metal-decorated SMOs-based chemiresistive gas sensors are proposed.

关键词： Noble metal Bimetal Semiconducting metal oxide Chemiresistive gas sensor Electronic sensitization Chemical sensitization

伊曲康唑治疗重症肝病并发肺部真菌感染患者的临床疗效分析

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中西医结合肝病杂志 2021年第7期31卷 616-618页

作者：陈学兵欧世梅张利沙德阳市人民医院四川德阳618000 德阳市第二人民医院

目的:探讨伊曲康唑治疗重症肝病并发肺部真菌感染患者的临床效果。方法:选取2016年1月至2019年2月在我院治疗的重症肝病并发肺部真菌感染患者89例,根据治疗方案分为试验组(n=44)和对照组(n=45),试验组患者给予伊曲康唑治疗,对照组患者... 详细信息

目的:探讨伊曲康唑治疗重症肝病并发肺部真菌感染患者的临床效果。方法:选取2016年1月至2019年2月在我院治疗的重症肝病并发肺部真菌感染患者89例,根据治疗方案分为试验组(n=44)和对照组(n=45),试验组患者给予伊曲康唑治疗,对照组患者给予氟康唑治疗,疗程28 d,检测治疗前后患者细胞免疫功能及胸部影像学变化,对比两组患者治疗疗效、不良反应。结果:试验组患者疗效优于对照组(P0.05)。结论:伊曲康唑治疗重症肝病并发肺部真菌感染患者有较好的临床效果,值得临床推广。

关键词：伊曲康唑重症肝病肺部真菌感染临床效果

血清QSOX1水平及其基因多态性与乙肝病毒性肝癌的遗传易感性研究

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临床和实验医学杂志 2021年第1期20卷 12-15页

作者：欧世梅陈学兵张利沙德阳市人民医院耳鼻喉头颈外科四川德阳618000 德阳市人民医院感染科四川德阳618000 德阳市第二人民医院检验科四川德阳618000

目的探讨血清巯基氧化酶1(QSOX1)水平及其基因多态性与乙肝病毒性肝癌的遗传易感性的相关性。方法本研究采用病例-对照研究方法,回顾性收集德阳市人民医院收治的200例乙肝病毒性肝癌患者(HCC)及200例种族、年龄、性别匹配的对照组为研... 详细信息

目的探讨血清巯基氧化酶1(QSOX1)水平及其基因多态性与乙肝病毒性肝癌的遗传易感性的相关性。方法本研究采用病例-对照研究方法,回顾性收集德阳市人民医院收治的200例乙肝病毒性肝癌患者(HCC)及200例种族、年龄、性别匹配的对照组为研究对象。首先利用酶联免疫吸附法(ELISA)试剂盒检测两组人群血清中QSOX1的含量;利用构象差异凝胶电泳的方法将QSOX1基因启动子区的rs11589519和rs7597位点的单核苷酸多态性进行分型;利用卡方检验判断QSOX1基因型分布频率是否符合遗传平衡定律;同时分析QSOX1基因启动子区不同位点的基因多态性和等位基因型与HCC遗传易感性的相关性。分析QSOX1基因启动子区的rs11589519和rs7597位点基因多态性与HCC患者肿瘤分期的相关性。结果 HCC组血清中QSOX1的表达含量(3.48±1.82)明显高于对照组(1.91±0.78),差异有统计学意义(P 0.05)。HCC组QSOXl位点rs11589519基因型TT频率高于对照组(38.0%vs.25.0%),rs7597基因型CT、TT频率高于对照组(48.0%vs.16.0、32.0%vs.10.0%),差异有统计学意义(P <0.05)。HCC组QSOXl基因rs11589519和rs75972位点等位基因型T分布频率均高于对照组(65.0%vs.51.0%、56.0%vs.18.0%),差异有统计学意义(P <0.05)。QSOX1基因启动子区的rs11589519位点AA型HCC患者IIIa+IIIb比率明显高于TT型患者(P <0.05)。QSOX1基因启动子区的rs7597位点TT型HCC患者Ⅲa+Ⅲb比率明显高于CC型患者,差异有统计学意义(P <0.05)。结论 QSOX1基因启动子区的rs11589519和rs7597位点多态性与HCC遗传易感性存在相关性。

关键词：乙肝病毒性肝癌巯基氧化酶1 多态性遗传易感性

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表皮屏障受损对接触致敏物的反应

中国皮肤性病学杂志 2017年第12期31卷 1292-1296页

作者：秦鸥王学民谈益妹江文才上海市皮肤病医院上海200443

目的探讨受到微弱损伤的皮肤致敏后,发生变应性接触性皮炎的几率及对皮肤的影响。方法采用十二烷基磺酸钠敷贴,胶带粘贴及紫外线照射三种方式对皮肤进行微弱损伤并用芳香混合物Ⅰ致敏,通过临床评估,非创仪器Tewameter~TM210,Corneomet... 详细信息

目的探讨受到微弱损伤的皮肤致敏后,发生变应性接触性皮炎的几率及对皮肤的影响。方法采用十二烷基磺酸钠敷贴,胶带粘贴及紫外线照射三种方式对皮肤进行微弱损伤并用芳香混合物Ⅰ致敏,通过临床评估,非创仪器Tewameter~TM210,Corneometer~CM820,Chromameter~CR400和实验室检查评价皮肤表面状况,检测皮肤经表皮失水率、含水量、颜色和炎症及致敏情况。结果进行微弱损伤后,临床评估为0分,但十二烷基磺酸钠和胶带粘贴部位经表皮失水率显著高于微损伤前,十二烷基磺酸钠部位含水量显著低于微损伤前(P<0.05)。致敏后,十二烷基磺酸钠和胶带粘贴部位的临床评分、经表皮失水率和a~*值均比未受过微损伤的致敏部位显著升高(P<0.05);IL-1α和IL-12的含量较致敏前显著升高(P<0.05)。以上差异均有统计学意义。结论及早发现皮肤屏障受到的微弱损伤,能及时阻断无临床症状的皮肤向临床疾病方向发展,预防接触性皮炎的产生和发展。

关键词：表皮屏障微弱损伤接触致敏

Potential effects of the nursing work environment on the work-family conflict in operating room nurses

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World Journal of Clinical Cases 2021年第26期9卷 7738-7749页

作者： Chun-mei Fu Jie ou Xiao-mei Chen mei-Ye Wang Department of Ophthalmology Hainan West Central HospitalDanzhou 571700Hainan ProvinceChina Department of Critical Care Medicine Hainan General HospitalHaikou 570311Hainan ProvinceChina Operation Room The Second Affiliated Hospital of Hainan Medical CollegeHaikou 570311Hainan ProvinceChina Department of General Practice Hainan West Central HospitalDanzhou 571700Hainan ProvinceChina

BACKGRouND The nursing working environment is an important subsystem in the hospital environment.A good working environment could have a positive impact on nurses.However,the work-family conflict and unsatisfactory working environment could significantly reduce their working enthusiasm,efficacy as well as the overall quality of the nursing,increase their fatigue,and thereby compromise their career status.AIM To explore the possible status quo and to analyze the correlation between work environment perception and the work-family conflict among nurses in the operating room.METHODS A total of 312 operating room nurses from two first-class hospitals at Grade 2 and two first-class hospitals at Grade 3 in China from May to September 2017 were included in this research using the cluster sampling method.The data,including the general information questionnaire,the practice environment scale of the nursing work index(PES-NWI),and the work-family conflict scale,were systematically collected.Pearson correlation analysis was applied to analyze the correlation between the two scores,with influencing factors analyzed by hierarchical regression analysis.RESULTS A total of 312 questionnaires were issued,and the response rate and effective questionnaire rate were both 96.15%(300/312).The total scores of the PES-NWI scale and the work-family conflict scale were 3.07±0.43(vs maximum up to 4 points)and 52.32±8.79(vs maximum up to 90 points),respectively.The scores of the PES-NWI scale were negatively correlated with that of work-family conflict scale(all Pound at a medium level with a negative correlation between the two.

关键词： Operating room nurses Perception of nursing work environment Workfamily conflict Correlation Influencing factors

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具有超疏水表面的硅/二氧化硅层次结构薄膜

物理化学学报 2011年第9期27卷 2233-2238页

作者：王建涛张晓宏王辉欧雪梅中国科学院理化技术研究所光化学转换和功能材料重点实验室北京100190 中国科学院研究生院北京100049

目前报道的硅基材料的超疏水表面主要是通过制备粗糙微观结构, 并在其表面修饰表面能相对较低的有机物两个步骤来实现的, 在户外等实际环境中应用时存在由于表面修饰有机物的降解而逐渐失去超疏水性的问题. 本工作以液态金属锡作为生长... 详细信息

目前报道的硅基材料的超疏水表面主要是通过制备粗糙微观结构, 并在其表面修饰表面能相对较低的有机物两个步骤来实现的, 在户外等实际环境中应用时存在由于表面修饰有机物的降解而逐渐失去超疏水性的问题. 本工作以液态金属锡作为生长衬底, 通过化学气相沉积(CVD)法制备了一种具有超疏水性能的硅基薄膜结构. 利用扫描电镜(SEM), 透射电镜(TEM)以及X射线衍射(XRD)等手段对产物的表面形貌和组成结构进行分析发现, 薄膜表面由竖直生长的硅/二氧化硅(Si/SiO2)核壳层次结构组成. 采用Cassie理论模型对其超疏水性能的产生提出了可能的解释. 发现构成薄膜表面的Si/SiO2层次结构单元的形貌是影响超疏水性能的重要因素. 相对于以前报道的硅基材料的超疏水表面, 这种新结构的超疏水性能不依赖于表面化学修饰, 有望拓宽硅基材料的应用环境.

关键词：超疏水核壳结构硅纳米线化学气相沉积薄膜

萤火虫与灰阶成像对甲状腺钙化诊断的对比分析

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中国医科大学学报 2009年第9期38卷 717-719页

作者：欧国成熊毅王学梅中国医科大学附属第一医院超声诊断科沈阳110001

目的探讨萤火虫技术对甲状腺中钙化诊断的应用价值。方法选取患有甲状腺结节性疾病的患者44例,术前对甲状腺结节分别应用灰阶及萤火虫技术进行超声检查,对二者的超声图像进行对比分析。结果手术切除73个结节,5个恶性,68个良性;灰阶图像... 详细信息

目的探讨萤火虫技术对甲状腺中钙化诊断的应用价值。方法选取患有甲状腺结节性疾病的患者44例,术前对甲状腺结节分别应用灰阶及萤火虫技术进行超声检查,对二者的超声图像进行对比分析。结果手术切除73个结节,5个恶性,68个良性;灰阶图像显示15个结节内有微钙化(5个呈砂砾样),17个结节内有粗钙化,6个结节内有可疑钙化,35个结节未显示钙化;萤火虫图像显示23个结节有微钙化,17个结节内有粗钙化,33个结节内未显示钙化。结论灰阶成像与萤火虫成像对甲状腺结节内钙化的检出率都很高,但萤火虫成像显示的钙化更清晰、明了,能鉴别疑似钙化的真伪,能显示灰阶图像无法辨认的特别小的钙化;但对假性钙化的敏感性同样高。

关键词：超声萤火虫技术甲状腺钙化

Decreased expression of human aquaporin-5 correlated with mucus overproduction in airways of chronic obstructive pulmonary disease

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Acta Pharmacologica Sinica 2007年第8期28卷 1166-1174页

作者： Ke WANG Yu-lin FENG Fu-qiang WEN~2 xue-rong CHEN xue-mei ou Dan XU Jie YANG Zhi-ping DENG Division of Pulmonary Diseases,State Key Laboratory of Biotherapy of China,and Department of Respiratory Medicine,West China Hospital of Sichuan University,Sichuan 610041,China Division of Pulmonary Diseases and Department of Respiratory Medicine State Key Laboratory of Biotherapy of China West China Hospital of Sichuan University Sichuan China

Aim:To investigate the relationship between aquaporin-5(AQP5)expression andmucus overproduction in the airways of Chinese patients with chronic obstruc-tive pulmonary disease(COPD)and the correlation with pulmonary functionchange.Methods:Bronchial tissues were obtained from fiberoptic bronchoscopyand bronchial biopsy in West China Hospital from April to July 2004.Twenty-fivepatients were diagnosed as COPD patients,and another 20 were diagnosed as thecontrol patients.The expressions ofAQP5,mucin 5AC(MUC5AC),and mucin inbronchial tissues were detected by semiquantitative RT-PCR,in situ hybridization,and immunohistochemical and alcian blue-periodic acid-Schiff(AB-PAS)staining,respectively.Results:Compared with the control group,an attenuated expres-sion of AQP5was detected throughout the bronchial tissues from patients withCOPD(P0.05).Simultaneously,increased staining of MUC5AC and mucus in submucosal glandswere noted(Poups(Poup(P>0.05).The decreased expression of AQP5mRNA was correlatedwith forced expiratory volume in 1 s(FEV1)/forced vital capacity(FVC)(r=0.60,P<0.01),FEV1% predicted value(r=0.60,P<0.01),maximal expiratory flow in 50%vital capacity(V50)% predicted value(r=0.55,P<0.01),and maximal expiratory flowin 25% vital capacity(V25)% predicted value(r=0.45,P<0.01).The decreasedexpression was negatively correlated with MUC5AC mRNA of the epitheliumairways(r=-0.45,P<0.01)and the AB-PAS-stained area of submucosal glands(r=-0.61,P<0.01).The upregulation of MUC5AC mRNA correlated with the posi-tively AB-PAS-stained area of submucosal glands and correlated negativelywith FEV1/FVC(r=-0.53;P<0.01),FEV1% predicted value(r=-0.53;P<0.01),V50%predicted value(r=-0.48;P<0.01),and V25% predicted value(r=-0.43;P<0.01).Conclusion:The attenuated gene express

关键词： aquaporin-5 mucin 5AC mucin mucus overproduction chronic obstructive pulmonary disease