Decreased expression of human aquaporin-5 correlated with mucus overproduction in airways of chronic obstructive pulmonary disease

作     者：Ke WANG Yu-lin FENG Fu-qiang WEN~2 Xue-rong CHEN Xue-mei OU Dan XU Jie YANG Zhi-ping DENG Division of Pulmonary Diseases,State Key Laboratory of Biotherapy of China,and Department of Respiratory Medicine,West China Hospital of Sichuan University,Sichuan 610041,China 

作者机构：Division of Pulmonary DiseasesState Key Laboratory of Biotherapy of Chinaand Department of Respiratory MedicineWest China Hospital of Sichuan UniversitySichuan 610041China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2007年第28卷第8期

页      面：1166-1174页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 10[医学] 

基　　金：This study was supported by grants from the National Natural Science Foundation of China(No 30425007,30370627) from the China Medical Board of New York to Dr Fu-qiang WEN(No 00-722). 

主　　题：aquaporin-5 mucin 5AC mucin mucus overproduction chronic obstructive pulmonary disease 

摘      要：Aim:To investigate the relationship between aquaporin-5(AQP5)expression andmucus overproduction in the airways of Chinese patients with chronic obstruc-tive pulmonary disease(COPD)and the correlation with pulmonary functionchange.Methods:Bronchial tissues were obtained from fiberoptic bronchoscopyand bronchial biopsy in West China Hospital from April to July 2004.Twenty-fivepatients were diagnosed as COPD patients,and another 20 were diagnosed as thecontrol patients.The expressions ofAQP5,mucin 5AC(MUC5AC),and mucin inbronchial tissues were detected by semiquantitative RT-PCR,in situ hybridization,and immunohistochemical and alcian blue-periodic acid-Schiff(AB-PAS)staining,respectively.Results:Compared with the control group,an attenuated expres-sion of AQP5was detected throughout the bronchial tissues from patients withCOPD(P0.05).Simultaneously,increased staining of MUC5AC and mucus in submucosal glandswere noted(P0.05).The decreased expression of AQP5mRNA was correlatedwith forced expiratory volume in 1 s(FEV1)/forced vital capacity(FVC)(r=0.60,P0.01),FEV1% predicted value(r=0.60,P0.01),maximal expiratory flow in 50%vital capacity(V50)% predicted value(r=0.55,P0.01),and maximal expiratory flowin 25% vital capacity(V25)% predicted value(r=0.45,P0.01).The decreasedexpression was negatively correlated with MUC5AC mRNA of the epitheliumairways(r=-0.45,P0.01)and the AB-PAS-stained area of submucosal glands(r=-0.61,P0.01).The upregulation of MUC5AC mRNA correlated with the posi-tively AB-PAS-stained area of submucosal glands and correlated negativelywith FEV1/FVC(r=-0.53;P0.01),FEV1% predicted value(r=-0.53;P0.01),V50%predicted value(r=-0.48;P0.01),and V25% predicted value(r=-0.43;P0.01).Conclusion:The attenuated gene expression of AQP5existed in the airways ofChinese COPD patients,which was complicated by mucus hypersecretion.Thedecreased expression of AQP5mRNA may be related to the severity of airflowobstruction.