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Role of cell-free DNA for predicting incidence and outcome of patients with ischemic stroke

World Journal of Neurology 2022年第1期8卷 1-9页

作者： nusrath fathima Sandhya Manorenj Sandeep Kumar Vishwakarma Aleem Ahmed Khan Central Laboratory for Stem Cell Research and Translational Medicine Centre for Liver Research and DiagnosticsDeccan College of Medical SciencesHyderabad 500058TelanganaIndia Department of Neurology Princess Esra HospitalDeccan College of Medical SciencesHyderabad 500002TelanganaIndia

Early diagnosis and prognosis of ischemic stroke remains a critical challenge in clinical settings.A blood biomarker can be a promising quantitative tool to represent the clinical manifestations in ischemic ***-free DNA(cfDNA)has recently turned out to be a popular circulating biomarker due to its potential relevance for diagnostic applications in a variety of *** bright outlook of cfDNA in clinical applications,very less is known about its origin,composition,or *** recent studies have identified cell-derived mitochondrial components including mitochondrial DNA(mtDNA)in the extracellular spaces including blood and cerebrospinal ***,the time course of alterations in plasma mtDNA concentrations in patients after an ischemic stroke is poorly *** is thought to be freed into the plasma shortly after the commencement of an ischemic stroke and then gradually ***,the importance of cell-free mtDNA(cf-mtDNA)in ischemic stroke is still *** review summarizes about the utility of biomarkers which has been standardized in clinical settings and role of cfDNA including cfmtDNA as a non-invasive potential biomarker of ischemic stroke.

关键词： Ischemic stroke Mitochondria Circulating cell-free DNA Plasma nucleic acid Mortality

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Cell-free mitochondrial DNA quantification in ischemic stroke patients for non-invasive and real-time monitoring of disease status

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World Journal of Translational Medicine 2022年第2期10卷 14-28页

BACKGROUND Acute ischemic stroke(AIS)is one of the major causes of the continuous increasing rate of global mortality due to the lack of timely diagnosis,prognosis,and *** study provides a primitive platform for non-invasive and cost-effective diagnosis and prognosis of patients with AIS using circulating cellfree mitochondrial DNA(cf-mtDNA)quantification and *** To evaluate the role of cf-mtDNA as s non-invasive,and affordable tool for realtime monitoring and prognosticating AIS patients at disease onset and during *** This study enrolled 88 participants including 44 patients with AIS and 44 healthy controls with almost similar mean age group at stroke onset,and at 24 h and 72 h of *** blood samples were collected from each study participant and plasma was separated using *** cf-mtDNA concentration was quantified using nanodrop reading and validated through real-time quantitative polymerase chain reaction(RT-qPCR)of NADH-ubiquinone oxidoreductase chain 1(ND1)relative transcript expression *** Comparative analysis of cf-mtDNA concentration in patients at disease onset showed significantly increased levels compared to control individuals for both nanodrop reading,as well as ND1 relative expression levels(P<0.0001).Intergroup analysis of cf-mtDNA concentration using nanodrop showed significantly reduced levels in patients at 72 h of treatment compared to onset(P<0.01).However,RT-qPCR analysis showed a significant reduction at 24 h and 72 h of treatment compared to the disease onset(P<0.001).The sensitivity and specificity were relatively higher for RT-qPCR than nanodrop-based cfmtDNA *** analysis of both cf-mtDNA concentration as well as ND1 relative expression with National Institute of Health Stroke Scale score at baseline showed a positive *** In summary,quantitative estimation of highly pure cf-mtDNA provides a simple,highly sensitive and specific,no

关键词： Cell-free mitochondrial DNA NADH-ubiquinone oxidoreductase chain 1 Ischemic stroke Circulating biomarker National Institute of Health Stroke Scale score Stroke assessment Severity and outcome

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Synthesis, evaluation of kinetic characteristics and investigation of apoptosis of Cu^(2+)-modified ceria nano discs

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Journal of Rare Earths 2018年第10期36卷 1050-1059页

作者： K.nusrath K.Muraleedharan Department of Chemistry University of Calicut

Ceria nano discs were synthesized by the stepwise thermal decomposition strategy of the oxalate precursor. A series of Ce（1-x）CuxO2（x = 0, 0.02, 0.1, 0.2 and 0.3） nano sized oxide systems were prepared through thermal decomposition route. Kinetic characterization of formation of solid solution was made by isoconversional strategy under non-isothermal condition. Introduction of various reactant molar ratios of Cu2＋：Ce4＋ has a pivotal role in the creation of new oxygen vacancies, decomposition strategy,particle size and shape. Cu2＋ doping（x = 0.02 and 0.1） damages the disc shaped morphology of *** distribution of Cu2＋ on the oxalate precursor has a significant role in the catalyzing activity for the destruction of oxalate bond to oxide. 2 mol% doped Cu2＋ promotes breaking of oxalate bonds in nitrogen atmosphere. In vitro cell viability assay illustrates enhanced toxicity to cancer cells with 10 mol% Cu2＋ doped ceria.

关键词： Nano disc Isoconversional Oxygen vacancies Decomposition strategy Cell viability Rare earths

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A Nutrient Mixture Inhibits MMP Secretion, Invasion, Growth, and Induction of Apoptosis in Human Tongue Cancer Cell Line SC-255

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Open Journal of Apoptosis 2013年第4期2卷 37-44页

作者： M. Waheed Roomi nusrath W. Roomi Bilwa Bhanap Aleksandra Niedzwiecki Matthias Rath Dr. Rath Research Institute Oncology Division Santa Clara USA

A majority of oral cancers is squamous cell carcinoma and tongue carcinomas comprise 30% of all oral cancers. However, Phytochemicals, herbal and dietary antioxidants have been reported to prevent cancers. A nutrient mixture containing, ascorbic acid, lysine, proline and green tea extract, among other nutrients, has previously been shown to exhibit a broad spectrum chemo preventative and therapeutic anti-cancer properties in a number of cell lines. In a recent study, we found that the nutrient mixture significantly inhibited acetaminophen induced hepatic and renal toxicity, and it suppressed carbon tetrachloride induced hepatic toxicity in ICR mice as well. This study was undertaken to determine if the nutrient mixture is useful in inhibiting various parameters of cancer progression on human tongue cancer cell line SC-255. SC-255 cells were grown in a Dulbecco’s Eagle medium and treated with the nutrient mixture at 0, 10, 50, 100, 500 and 1000 μg/ml, in triplicate. The nutrient mixture exhibited 20% and 30% toxicity at 500 and 1000 μg/ml, respectively. Zymography demonstrated the expression of MMP-2 and MMP-9;and PMA treatment further enhanced MMP-9 activity. The nutrient mixture inhibited the secretion of MMP-2 and MMP-9 in a dose-dependent fashion with a total inhibition at 500 μg/ml. Matrigel invasion was significantly reduced by 40%, 80% and 100% at 100, 500 and 1000 μg/ml, respectively. The nutrient mixture also inhibited cell migration and induced apoptosis in a dose response fashion. Thus, the nutrient mixture may have a potential in the tongue cancer treatment.

关键词： Oral Cancer Nutrient Mixture MMP Apoptosis

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