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Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats

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International Journal of Ophthalmology(English edition) 2016年第11期9卷 1568-1577页

作者： Seskoati Prayitnaningsih Hidayat Sujuti Maksum Effendi Aulia Abdullah Nanda Wahyu Anandita Febriani Yohana nur permatasari Mohamad Aris Widodo Department of Ophthalmology Faculty of MedicineBrawijaya University Pharmacology Laboratory Faculty of MedicineBrawijaya University

AIM： To evaluate effect of hypertension on retinal ganglion cell（RGC） apoptosis, intraocular pressure（IOP）,and the activation of endothelin-1（ET-1） signaling pathway in central retinal artery（CRA） in ***： The experimental study was performed on20 male Sprague Dawley rats that were divided into control group, and hypertension groups. The hypertension was induced by subcutaneous deoxycorticoacetate（DOCA）10 mg/kg twice a week and administered 0.9% Na Cl solution daily for 2, 6, and 10 wk. Blood pressure（BP） was measured using animal BP analyzer. IOP was measured by handheld tonometry. Retinal tissue preparations by paraffin blocks were made after enucleation. The expression of ET-1, e NOS, ET-1 receptor A（ETRA）, ET-1receptor B（ETRB）, and phosphorylated myosin light chain kinase（MLCK）, and caldesmon（Ca D） in CRA and RGC apoptosis were evaluated through immunofluorescent staining method then observed using laser scanning confocal microscopy. RESULTS： BP significantly increased in all of the hypertension groups compared to control（P =0.001）.Peak IOP elevation（7.78±4.14 mm Hg） and RGC apoptosis（576.15±33.28 Au） occurred on 2wk of hypertension. ET-1expression（1238.6±55.1 Au） and e NOS expression（2814.2±70.7 Au） were found highest in 2wk of hypertension,although the ratio of ET-1/e NOS decreased since *** peak expression in 10 wk of hypertension（1219.4 ±6.3 Au）, while ETRBsignificantly increased only in 2 weeks group（1069.2 ±9.6 Au）. The highest MLCK expression（1190.09±58.32 Au）, Ca D（1670.28±18.36 Au）were also found in 2wk of ***： Hypertension effects to activation of ET-1 signaling pathway significantly in CRA, elevation of IOP, and RGC apoptosis. The highest value was achieved at 2wk, which is the development phase of hypertension.

关键词： hypertension central retinal artery endothelin- 1 signaling pathway rats

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谷胱甘肽和烟酸对高糖状态下人晶状体上皮细胞的影响（英文）

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国际眼科杂志 2020年第1期20卷 4-8页

作者： Nina Handayani nur permatasari Hidayat Sujuti Achmad Rudijanto 玛琅Saiful Anwar医院 Brawijaya大学医学院眼科印度尼西亚玛琅65145 Brawijaya大学医学院药理学系印度尼西亚玛琅65145 Brawijaya大学医学院生物化学与分子生物学系印度尼西亚玛琅65145 玛琅Saiful Anwar医院 Brawijaya大学医学院内科内分泌与代谢疾病科印度尼西亚玛琅65145

目的:评价还原性谷胱甘肽(GSH)和烟酸联合应用对蛋白质氧化应激、内质网应激(ER)、糖基化和聚合αβ晶状体蛋白的人晶状体上皮(HLE)细胞治疗高葡萄糖水平的影响。方法:培养HLE细胞并暴露于25 mmol/L葡萄糖中以促进高糖环境。各组细胞用... 详细信息

目的:评价还原性谷胱甘肽(GSH)和烟酸联合应用对蛋白质氧化应激、内质网应激(ER)、糖基化和聚合αβ晶状体蛋白的人晶状体上皮(HLE)细胞治疗高葡萄糖水平的影响。方法:培养HLE细胞并暴露于25 mmol/L葡萄糖中以促进高糖环境。各组细胞用三种不同的剂量组合进行联合治疗:10μmol/L GSH+2μmol/L烟酸,30μmol/L GSH+25μmol/L烟酸,100μmol/L GSH+25μmol/L烟酸。培养72h后,用ELISA法测定蛋白羰基(PCC)和葡萄糖反应蛋白(GRP78)的含量。培养2wk后,对糖基化终末产物(AGEs)进行检测,用Western Blot检测αβ晶状体蛋白的表达。所有数据分析均采用SPSS 18.0统计软件包。结果:与对照组相比,联合治疗组的PCC和GRP78水平没有显著降低(P>0.05)。相反,与对照组相比,GSH和烟酸联合治疗组的AGEs水平明显下降(P<0.05)。此外,高剂量葡萄糖给药后αβ晶状体蛋白表达增加,但GSH联合治疗组与GSH和烟酸联合治疗组αβ晶状体蛋白表达都有所降低。结论:结果表明,GSH和烟酸联合应用可抑制高血糖人HLE细胞蛋白质的聚合,防止糖基化,并且通过AGEs途径在预防糖尿病性白内障中发挥积极作用。

关键词：蛋白羰基葡萄糖反应蛋白糖基化终末产物 αβ晶状体蛋白糖尿病性白内障

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Effect of ethanolic extract of Physalis minima on lowering blood pressure in DOCA-salts hypertensive rat models

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中国药理学与毒理学杂志 2015年第S1期29卷 33-33页

作者： nur permatasari Dian NUGRAHENNY Saifurrohman Department of Pharmacology Faculty of Medicine Brawijaya University Indonesia Department of Internal Medicine Faculty of Medicine Brawijaya University Indonesia

OBJECTIVE To explore the effect of ethanolic extract of Physalis minima on lowering blood pressure in the DOCA-salts hypertensive *** To produce hypertension,male rats were induced DOCA at dose of 10mg·kg-1 *** weekly and 0.9% NaCl in drinking water ad libitum for six *** ethanolic extract of Physalis minima were given at dose of 500,1500 and 2500mg·kg-1,respectively for *** completion of treatment schedule,systolic blood pressure was evaluated using animal blood pressure *** serum nitric oxide levels was measured using the ELISA *** activity and MDA level were determined *** dysfunction was determined by acetylcholine-induced vasorelaxation studies on isolated rat *** The extract decreased systolic blood pressure significantly(P<0.001)in hypertensive *** addition,the extract also suppressed the production of MDA and restored SOD activity to normal *** was a reduced relaxation response to acetylcholine in isolated aortic rings and serum NO level from hypertensive rats that were reversed by *** Physalis minima can lower blood pressure in hypertensive rats and its mechanism may be through the improvement of endothelial function due to the decrease of stress oxidative.

关键词： physalis minima blood pressure endothelial cell st

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Modeling of the Rate of Weight Loss in the Pyrolysis of Waste of Tobacco Stem for the Development of Charcoal Briquette

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材料科学与工程（中英文B版） 2011年第4期1卷 479-484页

作者： Suryo Purwono Bardi Murachman Joko Wintoko nur Esti permatasari Diah Lidyawati Chemical Engineering Department Gadjah Mada University Jl. Grafika No. 2 Yogyakarta Indonesia

Waste of tobacco stem can be utilized as an energy alternative as a charcoal briquette. In the tobacco stem, there is carbon element bonded in the cellulose and can be utilized as an energy source of solid fuel. Charcoal briquette from tobacco stem can be developed via pyrolysis process. The mechanism of the weight loss due to pyrolysis reaction is an important problem and occurs in many industrial practices. The basic information that is of interest to the designer and to the operator of pyrolysis process are the conditions that cause carbonaceous materials to occur, and the effect of the fouling deposit on the transfer of heat and on the pressure drop in the flow system. The information must be related to time and system temperatures. The formation of carbonaceous material is usually to be a transient condition at least in the first stages of pyrolysis. The main objective of this paper is to find model of weight loss or reactivity of the pyrolysis process that will accurately predict gasification rate under different temperatures. This steady state kinetic study can be used to support the mathematical modeling since a suitable reaction rate expression is essential for a successful reactor model. A model of the weight loss during pyrolysis process was developed. The model developed appears to be applicable to this pyrolysis experiment for the development of charcoal briquette from the waste of tobacco stem. This can be demonstrated with the experimental data obtained from the pyrolysis processes at two different temperature. Straight lines passing through the data points for each of the samples indicate clearly that the model properly describe the pyrolysis process.

关键词：热解过程数学建模烟梗木炭型煤失重率裂解过程裂解反应

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