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cis-trans Engineering： Advances and Perspectives on Customized Transcriptional Regulation in Plants

Molecular Plant 2018年第7期11卷 886-898页

作者： Ankita Shrestha Ahamed Khan nrisingha dey Division of Microbial and Plant Biotechnology Institute of Life Sciences Department of Biotechnology Government of India Chandrasekharpur Bhubaneswar Odisha India These authors contributed equally to this article.

Coordinated transcriptional control employing synthetic promoters and transcription factors （TFs） can be used to achieve customized regulation of gene expression in planta. Synthetic promoter technology has yielded a series of promoters with modified cis-regulatory elements that provide useful tools for efficient modulation of gene expression. In addition, the use of zinc fingers （ZFs）, transcription activator-like effectors （TALEs）, and catalytically inactive clustered regularly interspaced short palindromic repeats （CRISPR）-associated protein 9 （dCas9） has made it feasible to engineer TFs that can produce targeted gene expression regulation; these approaches are particularly effective when artificial TFs are coupled with transcriptional activators or repressors. This review focuses on strategies used to engineer both promoters and TFs in the context of targeted transcriptional regulation. We also discuss the creation of synthetic inducible platforms, which can be used to impart stress tolerance to plants. We propose that combinatorial ＂cis-trans engineering＂ using a CRISPR-dCas9-based bipartite module could be used to regulate the expression of multiple target genes. This approach provides an attractive tool for introduction of specific qualitative traits into plants, thus enhancing their overall environmental adaptability.

关键词： cis engineering trans engineering synthetic TFs dCas9 bipartite module

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The Development of Efficient Plant Promoters for Transgene Expression Employing Plant Virus Promoters

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Molecular Plant 2012年第4期5卷 941-944页

作者： Sunita Patro Deepak Kumar Rajiv Ranjan Indu B.Maiti nrisingha dey Institute of Life Sciences Department of BiotechnologyGovernment of IndiaNalco SquareChandrasekherpurBhubaneswar 751023OrissaIndia Kentucky Tobacco Research and Development Center University of KentuckyLexingtonKY 40546-0236USA

Dear Editor, The structure of eukaryotic promoter is modular, consisting of different sub-domains （Benfey et al., 1990）. Cross-talk among these sub-domains and effective combinatorial interac- tions between the specific cis-element/s with respective trans- factor/s usually control the strength and tissue specificity of the promoter. Further, specificity/inducibility of promoter can be modified by altering its genetic architecture through ＇cis-rearrangement＇（Rushton et al., 2002） and ＇swapping of sub-domains＇（Bhullar et al., 2003）. The fundamental motiva- tion behind developing such modified promoters lies in thebelief that the swapping/shuffling of the upstream activation sequences carrying a specific set of cis-elements that bind to a particular trans-factor from one promoter to the other con- taininq a TATA seauence that miaht result in a novel chimericregu!atory module,

关键词： GENE expression in plants PROMOTERS (Genetics) GENE expression CHARTS, diagrams, etc. CHIMERIC nucleic acids PLANT molecular virology GENOMICS

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Implications of Nanobiosensors in Agriculture

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Journal of Biomaterials and Nanobiotechnology 2012年第2期3卷 315-324页

作者： Vineeta Rai Sefali Acharya nrisingha dey Institute of Life Sciences Division of Gene Function and Regulation Laboratory of Plant Biotechnology Bhubaneswar (Odisha) India

Nanotechnology has emerged as a boon to the society with immense potential in varied area of research and our day-to-day life. The application of nanotechnology for the advancement of biosensor leads to an efficient nanobiosensor with miniature structure as compared to conventional biosensors. Nanobiosensors can be effectively used for sensing a wide variety of fertilizers, herbicide, pesticide, insecticide, pathogens, moisture, and soil pH. Taken together, proper and controlled use of nanobiosensor can support sustainable agriculture for enhancing crop productivity.

关键词： Nanobiotechnology Nanobiosensors Sustainable Agriculture

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