cis-trans Engineering： Advances and Perspectives on Customized Transcriptional Regulation in Plants
cis-trans Engineering： Advances and Perspectives on Customized Transcriptional Regulation in Plants作者机构：Division of Microbial and Plant Biotechnology Institute of Life Sciences Department of Biotechnology Government of India Chandrasekharpur Bhubaneswar Odisha India These authors contributed equally to this article.
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2018年第11卷第7期
页 面：886-898页
核心收录：
学科分类：0710[理学-生物学] 12[管理学] 1201[管理学-管理科学与工程(可授管理学、工学学位)] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：supported by funds from the Government of India
主 题：cis engineering trans engineering synthetic TFs dCas9 bipartite module
摘 要：Coordinated transcriptional control employing synthetic promoters and transcription factors （TFs） can be used to achieve customized regulation of gene expression in planta. Synthetic promoter technology has yielded a series of promoters with modified cis-regulatory elements that provide useful tools for efficient modulation of gene expression. In addition, the use of zinc fingers （ZFs）, transcription activator-like effectors （TALEs）, and catalytically inactive clustered regularly interspaced short palindromic repeats （CRISPR）-associated protein 9 （dCas9） has made it feasible to engineer TFs that can produce targeted gene expression regulation; these approaches are particularly effective when artificial TFs are coupled with transcriptional activators or repressors. This review focuses on strategies used to engineer both promoters and TFs in the context of targeted transcriptional regulation. We also discuss the creation of synthetic inducible platforms, which can be used to impart stress tolerance to plants. We propose that combinatorial ＂cis-trans engineering＂ using a CRISPR-dCas9-based bipartite module could be used to regulate the expression of multiple target genes. This approach provides an attractive tool for introduction of specific qualitative traits into plants, thus enhancing their overall environmental adaptability.
维普期刊数据库