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Antifungal activity of four honeys of different types from Algeria against pathogenic yeast:Candida albicans and Rhodotorula sp.

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Asian Pacific Journal of Tropical Biomedicine 2012年第7期2卷 554-557页

作者： Ahmed Moussa djebli noureddine Aissat Saad Meslem Abdelmelek Benhalima Abdelkader Institute of Veterinary Sciences University Department of Biology Faculty of SciencesWostaganem University Laboratory Science and Technology Environment and Development Mostaganem University

Objective:To evaluate the antifungal activity of four honeys of different types from Algeria against pathogenic yeast *** albicans(***) and Rhoddtorula ***:Four Algeria honeys of different botanical origin were analvzed to test anlilungal effect against ***,and Rhodotorula *** concentrations(undiluted,10%,30%,50%and 70%w/v) of honey were studied in vitro for their antifugal aclivity using *** and Rhodotorula *** fungal ***:The range of the c liameter of zone of inhibition of various concentrations ol tested honeys was(7-23 mm) for Rhodotorula sp.,while *** showed clearly resistance towards all concentrations *** MICs of tested honey concentrations against *** and Rhodotorula ***(70.09-93.48)%and(4.90-99.70)%v/v,***:This study demonstrales that,in vitro,these natural products have clearly an antifungal activity against Rhodotorula *** ***.

关键词： Honey Antifungal activity Candida albicans Rhodotorula sp.

In vitro activity of natural honey alone and in combination with curcuma starch against Rhodotorula mucilaginosa in correlation with bioactive compounds and diastase activity

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Asian Pacific Journal of Tropical Biomedicine 2013年第10期3卷 816-821页

作者： Moussa Ahmed noureddine djebli Saad Aissat Baghdad Khiati Abdelmalek Meslem Salima Bacha Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem.UniversityAlgeria

Objective:To evaluate the in vitro activity and synergism of the combinations of natural honey and curcuma starch against Rhodotorula mucilaginosa in correlation with total phenolic,flavonoid contents,and diastase ***:The Folin-Ciocalteu test was used to determine the total polyphenols content and the flavonoid content was analyzed using by the aluminum chloride *** antifungal activity of the natural honey,determined by an agar well diffusion assay and agar incorporation ***:Total phenolic content varied from(63.93±0.11)to(95.36±6.08)mg GAE/100 g honey as gallic acid *** flavonoids content varied from(5.41±0.04)to(9.94±0.54)mg CE/*** activity values were between(7.3±2.8)and(26±2.8).The zone inhibition diameter for the six honey samples without starch ranged between 6 and 20 *** starch was mixed with honey and then added to well,a zone inhibition increase diameter 7 and 21 *** percentage increase was noticed with each variety and it ranged between 5%and 62.5%.The minimal inhibitory concentrations for the six varieties of honey without starch against Rhodotorula mucilaginosa ranged between 28%and 36%(v/v).When starch was incubated with honey and then added to media,a minimal inhibitory concentration drop has been noticed with each *** ranged between 6.66%and 20%(w/v).No significant correlation was established between diastase activity and bioactive ***:The mixture of curcuma starch and honey could lead to the development of new combination antibiotics against Rhodotorula

关键词： Bioactive Compounds Diastase number Rhodotorula mucilaginosa Honey Curcuma starch

Additive potential of ginger starch on antifungal potency of honey against Candida albicans

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Asian Pacific Journal of Tropical Biomedicine 2012年第4期2卷 253-255页

作者： Ahmed Moussa djebli noureddine Hammoudi SM Aissat Saad Akila Bourabeh Hemida Houari Laboratory of Hygiene and Animal Pathology Institute of Veterinary Sciences University Ibn-KhadounTiaret Departments of Biology Faculty of SciencesMostaganem University Institute of Veterinary Sciences University Ibn-Khadoun Tiaret

Objective:To evaluate the additive action of ginger starch on the antifungal activity of honey against Candida albicans(***).Methods:*** was used to determine the minimum inhibitory concentration(MIC)of four varieties of Algerian *** concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to detennine the minimum additive inhibitory concentration(MAIC).Results:The MIC for the four varieties of honey without starch against *** ranged between 38%and 42%(v/v).When starch was incubated with honey and then added to media,a MIC drop was noticed with each *** of the four varieties ranged between 32%honey(v/v)with 4%starch and 36%honey(v/v) with 2%***:The use of ginger starch allows honey benefit and will constitute an alternative way against the resistance to antifungal agents.

关键词： Honey Ginger starch Antifungal potency Candida albicans

Effect of heat treatment on antimycotic activity of Sahara honey

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Journal of Coastal Life Medicine 2014年第11期 876-881页

作者： Moussa Ahmed Saad Aissat noureddine djebli Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria

Objective:To evaluate the influence of the temperature on honey colour,polyphenol contents and antimycotic capacity and to evaluate the correlation between these ***:Sahara honey were heated up to 25,50,75 and 100℃for 15,30 and 60 min,and their colour intensity,polyphenol contents and antimycotic *** Folin-Ciocalteu test was used to determine the total polyphenol contents(TPC).The antimycotic activity was evaluated both by agar diffusion method and micro wells dilution method against the Candida albicans(***)and Candida glabrata(***).Results:Initial values for TPC in Sahara honey ranged from 0.55 to 1.14 mg of gallic acid per kg of honey,with the average value of 0.78 mg of gallic acid per kg of *** TPC values after heat-treatment were 0.54 to 1.54 with the average value of 1.49 *** minimal inhibitory concentrations before heat-treatment of Sahara honey against *** and *** ranged from 3.06%-12.5%and 50%*** heat-treatment the minimal inhibitory concentrations between 12.5%and 50%for *** and ***,*** diameters of inhibition zones of Sahara honey with 50%concentration varied from(12.67-15.00)mm by *** to(14.33-15.67)mm by *** diameters of inhibition zones after heat-treatment at 25 and 50°C for 15.30 and 60 min ranged from(2.00-18.67)mm by *** to(8.00-16.67)mm by *** significant relations between the TPC and the colour intensity of Sahara honey(r=0.99,P<0.05).Furthermore,the results showed that the TPC and colour is not correlated with the antimycotic ***:To our knowledge this is the first report on the antimycotic capacity of Sahara honey.

关键词： Sahara honey Antimycotic capacity Heat treatment

Preliminary comparative study of anti-inlfammatory effect of unheated and heat-treated Sahara honey:In vivo approach

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Journal of Coastal Life Medicine 2015年第11期 875-878页

作者： Moussa Ahmed Saad Aissat noureddine djebli Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria

Objective:To investigate the effect of unheated and heat-treated of Sahara ***:A total of 24 Swiss albino mice weighing 25-35 g were divided into four groups(n=6).Anti-inflammatory effect was assessed at 1,2,3,4,5 and 6 h after subplantar injection of carrageenan(0.5 mL of a 1%solution in normal saline).In addition,total phenolic content was determined by modified Folin-Ciocalteu ***:The total phenolic content capacity of the Sahara honey before and after heat treatment was between 72 and 97.9 mg of gallic acid equivalents/100 g of honey *** of unheated honey(oral administration)reduced significantly(P<0.05).The carrageenan induced mice paw edema model at 1,3 and 6 h for 21.85%,5.43%and 80.43%,*** of heat-treated honey showed insignificant inhibition of carrageenan and induced paw edema at 1 h(31.16%),3 h(0.25%)and 6 h(34.19%).The 50 mg/kg diclofenac exhibited percent reduction in paw volume 16.12%,8.90%and 15.32%after 1 h,3 h and 6 h,respectively,when compared with control *** toxicity was ***:Our results suggest that unheated Sahara honey has anti-inflammatory effects by reducing the mice paw edema size while heat-treated Sahara honey decreases the antiinflammatory activity.

关键词： Sahara honey Heating Anti-inflammatory Total phenolic content

Preliminary study on synergistic combinations of raw honey with gentamicin against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa of veterinary origin

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Journal of Coastal Life Medicine 2015年第5期 366-369页

作者： Moussa Ahmed Baghdad Khiati Saad Aissat noureddine djebli Pharmacognosy Api-Phytotherapy Laboratory Mostaganem UniversityMostaganemAlgeria Institute of Veterinary Sciences University Ibn-Khaldoun TiaretAlgeria

Objective:To search for further synergistic combinations of gentamicin and raw honey that might have potential in treating ***:The antibacterial activity and synergistic interaction of raw honey and gentamicin was assessed by using agar well diffusion *** Gram-negative(Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 2154)bacteria were selected for antibacterial activity *** cultures of bacteria were maintained in their appropriate agar slants at 4°C throughout the study and used as stock ***:Raw honey and gentamicin interacted synergistically to inhibit Escherichia coli and Pseudomonas ***:These results suggest that combinations of raw honey and gentamicin have therapeutic benefits in prophylaxis of infections caused by multidrug-resistant Gram-negative bacilli.

关键词： Honey Gentamicin Pseudomonas aeruginosa Escherichia coli