检索结果-南通市图书馆

Gold Nanoparticle-based Layer-by-Layer Enhancement of DNA Hybridization Electrochemical Signal at Carbon Nanotube Modified Carbon Paste Electrode

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第6期17卷 795-798页

作者： Li Bo NIE Jian Rong CheN Yu Qing MIAO nong yue he College of Chemistry and Life Science Zhejiang Normal University Jinhua 321004 Key Laboratory of Green-packaging and Application of Bionanotechnology of Hunan Province Hunan University of Technology Zhuzhou 412008 State Key Laboratory of Bioelectronics Southeast University Nanjing 210096

Colloid gold nanoparticle-based layer-by-layer amplification approach was applied to enhance the electrochemical detection sensitivity of DNA hybridization at carbon nanotube modified carbon paste electrodes （CNTPEs）. Streptavidin was immobilized onto the surface of CNTPEs, and the conjugation of biotin labeled target oligonucle,otides to the above immobilized streptavidin was performed, followed by the hybridization of target oligonucleotides with the gold nanoparticle-labeled DNA probe and then the layer-by-layer enhanced connection of gold nanoparticles, on which oligonucleotides complementary to the DNA probe were attached, to the hybridization system. The differential pulse voltammetry （DPV） signal of total gold nanoparticles was monitored. It was found that the layer-by-layer colloidal gold DPV detection enhanced the sensitivity by about one order of magnitude compared with that of one-layer detection. One-base mismatched DNA and complementary DNA could be distinguished clearly.

关键词： Carbon nanotubes carbon paste electrode gold nanoparticles layer-by-layer amplifycation sensitivity.

Electrochemical Detection of Single A-G Mismatch Using Biosens-ing Surface Based on Gold Nanoparticles

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2005年第1期3卷 47-51页

作者： Ren-Yun Zhang Xue-Mei Wang Sheng-Jin Gong nong-yue he Chien-Shiung Wu Laboratory National Lab of Molecular and Biomolecular Electronics Department of Biological Science and Medical Engineering Southeast University Nanjing 210096 China.

The study of small drug molecules interacting with nucleic acids is an area of intense research that has particular relevance in our understanding of relative mechanism in chemotherapeutic applications and the association between genetics （including sequence variation） and drug response. In this contribution, we demonstrate how the sequence-specific binding of an anticancer drug Dacarbazine （DTIC） to single base （A-G） mismatch could be sensitively detected by combining electrochemical detection with biosensing surface based on gold nanoparticles.

关键词： single base mismatch DTIC electrochemistry gold nanoparticles biosensor

SBA-15 Modified Carbon Paste Electrode for Rapid cTnI Detection with Enhanced Sensitivity

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第2期17卷 235-238页

作者： nong yue he Hui Shi GUO Di YANG Chun Rong GU Ji Nan ZHANG State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 Institute of Cardiovascular Disease First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province Zhuzhou Institute of Technology Zhuzhou 412008

A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode （SBA-MCPE） is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.

关键词： Cardiac troponin Ⅰ SBA-15 mesoporous materials carbon paste electrode rapid quantitative immunoassay anodic stripping voltammetric detection nano-gold sensitivity.

Carbon encapsulated magnetic nanoparticles produced by hydrothermal reaction

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2007年第4期18卷 487-490页

作者： nong yue he Ya Fei Guo Yan Deng Zhi Fei Wang Song Li Hong Na Liu State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China b Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province Zhuzhou Institute of Technology Zhuzhou 412008 China

Carbon encapsulated magnetic nanoparticles （CEMNs） were synthesized by heating an aqueous glucose solution containing Fe-Au （Au coated Fe nanoparticles） nanoparticles at 160-180 ℃ for 2 h. This novel hydrothermal approach is not only simple but also provides the surface of CEMNs with functional groups like--OH. The formation of carbon encapsulated magnetic nanoparticles was not favored when using pure Fe nanoparticles as cores because of the oxidation of Fe nanoparticles by 1-120 during the reaction and, therefore, the surfaces of the naked Fe nanoparticles had to be coated by Au shell in advance. TEM, XRD, XPS and VSM measurments characterized that they were uniform carbon spheres containing some embedded Fe-Au nanoparticles, with a saturation of 14.6 emu/g and the size of the typical product is -350 nm.

关键词： Carbon encapsulated magnetic nanoparticles Hydrothermal reaction Gold shell

STUDY　ON　TheRMAL　AND　HYDROTheRMAL　STABILITY　OF　heTEROATOM　MCM－41　MOLECULAR　SIEVES

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1996年第3期7卷 257-258页

作者： nong yue he Rui Wang Shu Lin Bao Qin Hua Xu(Department of Chemistry, Naming University, Nanjing 210093) Department of Chemistry Nanjing University Nanjing 210093

Five samples of SiMCM-41, AlSiMCM-41, FeSiMCM-41, LaSiMCM-41 andAILaSiNCM-41 were synthesised and their good thermal stability was proved-by TG/DTAand XRD. All the samples were thermal stable up to abode 1150-1160 K. LaSiMCM-41 andFeSiMCM-41 showed better hydrothermal stability. Due to the cllalinel diameter within 1.5-10 urn, the synthesis ofmesoPOrous molecularsieve MCM41 by Mobil in 1992 anew new light on the conventional concept of SyntheSisofarelitCslll. For its POtential application, the studies on this novel matchal family are veryactive reCently, but few papers t'.ere published about their thennal and hydrithennal stability.

关键词： MCM AND heTEROATOM

Application of Nanogoid Probe Coupled with Silver Enhancement in Rapid cTnI Colorimetric Immunoassay

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2005年第12期16卷 1625-1628页

作者： nong yue he Hui Shi GUO Di YANG Chun Rong GU Zhi Ping BIAN Wen Hui WAN Ji Nan ZHANG State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 Institute of Cardiovascular Disease First Affiliated Hospital of Nanjing Medical University Nanjing 210029

A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin I （cTnI） based on the concepts of one-step dual monoclonal antibody “sandwich” principle. The low density protein array, the nanogold probe, and the silver enhancement on the gold particle were provided. The whole detection procedure of the assay could be fulfilled within 40 min with the pretreated colloidal gold-labeled detection antibody and supporting substrate. The assay showed good specific response to cTnI with very low cross-reactivity ratio to the skeletal isoforms of troponin I （sTnI）, cardiac troponin T （cTnT）, and myoglobin. 588 serum samples were assayed simultaneously by enzyme-linked immuno sorbent assay （ELISA） and this colloidal gold method to test the validity of the method and the data were analyzed using the statistical package SPSS version 11.0 （SPSS Inc.）. There was no significant difference between these two assays （P=0.66〉0.05）. The agreement between this method （〉 or 〈0.3 ng/mL） and ELISA was 86%.

关键词： Cardiac troponin I,protein array,nanogold particles,silver enhancement,rapidquantitative immunoassay

Multiple genotyping based on multiplex PCR and microarray

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2016年第11期27卷 1661-1665页

作者： Xian-Bo Mou Zeeshan Ali Bo Li Tao-Tao Li Huan Yi Hong-Ming Dong nong-yue he Yan Deng Xin Zeng State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology Hunan University of Technology Zhuzhou 412007 China Nanjing Maternity and Child health Care Hospital Nanjing 210029 China

The genetic variability has obtained more and more attention in the process of diagnosis and treatment of tumors. herein, we have described a multiple genotyping method based on magnetic enrichment- multiplex PCR （MEM-PCR） and microarray technology. Monodisperse magnetic beads were fabricated and modified with streptavidin. Four loci on two genes （M235T and A-6G loci on AGT gene, A1298C and C677T loci on MTHFR gene） were selected to study single nucleotide polymorphisms （SNP）. Target sequences of these SNP loci were amplified using Cy3-1abeled primers through multiplex PCR in one tube after the templates were enriched and purified by functional magnetic beads （MB）. Four pairs of NH2- labeled probes, corresponding to each locus, were fixed on CHO-modified glass slide by covalent binding. Hybridization between target sequences and probes was performed under suitable conditions. The spotting locations on microarray and the ratio of fluorescence intensity, produced by different loci, were used to distinguish the SNP genotypes. Finally, three of gastric cancer samples were collected and genotvping analysis for these four SNP loci was carried out successfully simultaneously by this method.

关键词： Magnetic beads Multiplex PCR Microarray Multiple genotyping Gastric cancer

Effect of Templates on the Structure and Stability of Ti-TMS

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1999年第3期10卷 267-268页

作者： Qing DAI nong yue he Xing Li WANG Xun Wei SheN Yan GUO Chun Wei YUANI(National Laboratory of Molecular and Biomolecular Electronics)(Department of Chemistry and Chemical Engineering, Southeast University, Naming 210096) Natl. Lab. Molec. Biomol. E. Southeast University Nanjing 210096 China Dept. of Chem. and Chem. Engineering Southeast University Nanjing 210096 China

Pure Mesoporous TiO2 molecular sieves (Ti-TMS) were synthesized with different templates for the first time and the templates can be successfully removed by refluxing the samples in the EtOH/H2O/KOH solution. The XRD pattern and TEM confirm that the uniformity and stability of Ti-TMS2 synthesized with dodecylamine as template is obviously inferior to that of Ti-TMS1 prepared with dodecylphosphate and hexadecylphosphate as templates. Furthermore, after the templates were removed, the mesostructure of Ti-TMS1 was preserved to a great degree than that of Ti-TMS2.

关键词： TiO2 mesoporous molecular sieves template

在线全文

学校读者我要写书评

暂无评论

我国村镇银行研究热点及发展趋势分析

辽宁农业科学 2024年第2期 27-32页

作者：何静玥文军农万相广西大学农学院广西南宁530004

为系统分析我国村镇银行研究概况和关键词演变,该文结合Excel和Scimago Graphica,利用CiteSpace V对CSSCI数据库中村镇银行相关文献进行内容分析和计量分析。结果表明,我国村镇银行年发文量呈现先上升后下降的趋势,存在多学科交叉、跨... 详细信息

为系统分析我国村镇银行研究概况和关键词演变,该文结合Excel和Scimago Graphica,利用CiteSpace V对CSSCI数据库中村镇银行相关文献进行内容分析和计量分析。结果表明,我国村镇银行年发文量呈现先上升后下降的趋势,存在多学科交叉、跨地区和研究机构合作研究,但未形成核心合作研究群;村镇银行研究总体可归纳为村镇银行、市场定位、金融服务、风险应对4个方面,关键词演变具有强政策引导性。未来要基于现有研究成果和结构性重组工作进行多学科融合探索,增加基于国内外宏观经济政策的视角和底层运营制度设计和监管的研究,加强学者和研究结构之间的合作,稳妥化解村镇银行面临的金融风险。

关键词：村镇银行农村金融机构研究热点 CiteSpace 文献计量

维普期刊数据库博看期刊