A novel strategy for in situ maskless synthesis of biochips:Self-driving micro-fluid porous type printing (SMPTP)

作     者：Nong Yue He Ya Fei Guo Song Li Jian-Xin Tang 

作者机构：State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province Zhuzhou Institute of Technology Zhuzhou 412008 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2007年第18卷第1期

页      面：111-114页

核心收录：

学科分类：080903[工学-微电子学与固体电子学] 0710[理学-生物学] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Doctoral Program of Higher Education for Funding, (20050286014) Jiangsu Public Interests Research Project, (BM2003701) National Natural Science Foundation of China, NSFC, (60571001, 60571032, 90406016) 

主　　题：Typography Oligonucleotide In situ synthesis DNA microarrays Biochips 

摘      要：A novel maskless technique, self-driving micro-fluid porous type printing （SMPTP）, was reported to in situ synthesize oligonucleotide arrays on glass slide, which has the merits of low cost, high quality and simple craft. In SMPTP for fabricating gene- chips, porous fiber tubes with a number of nanometric or micron channels functioned as ＂active letters＂ and were assembled in designed patterns, which are identical to the distribution of monomers in each layer of the array, and four patterns were needed for each layer. By means of capillarity, the synthesis solution was automatically taken into porous tubes assembled in a printing plate and reached the surface. An oligonucleotide array of 160 features with four different 15-mer probes was in situ synthesized using this technique. The four specific oligonucleotide probes, including the matched and the mismatched by the fluorescent target sequence, gave obviously different hybridization fluorescent signals.