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Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma

World Journal of Hepatology 2010年第1期2卷 32-41页

作者： Azza E El Bassiouny Mona M Nosseir Mona K Zoheiry noha a ameen Ahmed M Abdel-Hadi Ibrahim M Ibrahim Suher Zada Abdel-Hakeem Saad El-Deen Nora E El-Bassiouni Department of Immunology Theodor Bilharz Research Institute Department of Pathology Central Laboratory Theodor Bilharz Research Institute Department of Hepato-Gastroenterology Theodor Bilharz Research Institute Biology Department American University Zoology Department Faculty of ScienceCairo University Department of Hematology Theodor Bilharz Research Institute

AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,*** wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal *** investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn't express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and *** D1 expressed nuclear staining that was sequentially increased from CHC to LC(P<0.01) to HCC(P<0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 *** expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied *** analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P<0.001) ,between Cyclin D1 and Cyclin E in HCCs(P<0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P<0.001) and between p21 and Rb/p105(P<0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC p

关键词： Chronic hepatitis C Liver cirrhosis Hepatocellular carcinoma Cell cycle Cyclin D1 Cyclin E p21 p27 Rb/p105

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Resource Assessment of Wind Energy Potential of Mokha in Yemen with Weibull Speed

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Computers, Materials & Continua 2021年第10期69卷 1123-1140页

作者： Abdulbaset El-Bshah Fahd N.Al-Wesabi ameen M.Al-Kustoban Mohammad Alamgeer Nadhem Nemri Majdy M.Eltahir Hany Mahgoub noha Negm Department of Electronic Engineering Faculty of EngineeringUniversity of Science and Technology UST-Sana’aYemen Department of Computer Science King Khalid UniversityMuhayel AseerKingdom of Saudi Arabia Faculty of Computer and IT Sana’a UniversitySana’aYemen Department of Electronic Faculty of EngineeringUSTSana’aYemen Department of Information Systems King Khalid UniversityMayahel AseerKSA Department of Computer Science King Khaled UniversityKSA&Faculty of Computers and InformationMenoufia UniversityEgypt Department of Computer Science King Khaled UniversityKSA&Faculty of ScienceDepartment of Mathematics and Computer ScienceMenoufia UniversityEgypt

The increasing use of fossil fuels has a significant impact on the environment and ecosystem,which increases the rate of *** the high potential of renewable energy sources in Yemen and other Arabic countries,and the absence of similar studies in the *** study aims to examine the potential of wind energy in Mokha *** was done by analyzing and evaluating wind properties,determining available energy density,calculating wind energy extracted at different altitudes,and then computing the capacity factor for a few wind turbines and determining the *** speed was verified as the closest to the average actual wind speed using the cube root,as this was verified using 3 criteria for performance analysis methods(R^(2)=0.9984,RMSE=0.0632,COE=1.028).The wind rose scheme was used to determine the appropriate direction for directing the wind turbines,the southerly direction was appropriate,as the winds blow from this direction for 227 days per year,and the average southerly wind velocity is 5.27 m/s at an altitude of 3 *** turbine selected in this study has a tower height of 100m and a rated power of 3.45 *** capacitance factor was calculated for the three classes of wind turbines classified by the International Electrotechnical Commission(IEC)and compared,and the turbine of the first class was approved,and it is suitable for the study site,as it resists storms more than *** daily and annual capacity of a single,first-class turbine has been assessed to meet the needs of 1,447 housing units in Mokha *** amount of energy that could be supplied to each dwelling was around 19 kWh per day,which was adequate to power the basic loads in the home.

关键词： Wind energy system probability density wind speed Weibull velocity Rayleigh velocity wind rose

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Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan

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Asian Pacific Journal of Tropical Medicine 2018年第8期11卷 495-500页

作者： Mubashir Hussain Shahzad Munir Abdullah Jalal Taj Ali Khan Niaz Muhammad Bahar Ullah Khattak Abdullah Khan Irfan Ahmed Zulqarnain Baloch Nawaz Haider Bashir Muhammad ameen Jamal Kashif Rahim Humaira Mazhar Maira Riaz noha Watany Vector Borne Diseases Lab Department of Microbiology Kohat University of Science and Technology Kohat Faculty of Plant Protection Yunnan Agricultural University Institute of Biotechnology and Genetic Engineering The University of AgriculturePeshawar Pakistan Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University College of Veterinary Medicine South China Agricultural University Department of Animal Breeding Genetics and Reproduction Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and Biotechnology College of Life Sciences Beijing Normal University Vector Biology Research Lab US Naval Medical Research Unit 3

Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.

关键词： Leishmania major Rodents Cutaneous leishmaniasis Domestic animals ITS 1 PCR Waziristan

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First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 785-788页

作者： Mubbashir Hussain Shahzad Munir Sultan Ayaz Bahar Ullah Khattak Taj Ali Khan Niaz Muhammad Muhammad Anees Hazir Rahman Muhammad Qasim Muhammad ameen Jamal Irfan Ahmed Kashif Rahim Humaira Mazhar noha Watanay Mohamed Kasbari Vector Borne Diseases Laboratory Department of MicrobiologyKohat University of Science and Technology Kohat Department of Plant Pathology Faculty of Plant ProtectionYunnan Agricultural University College of Animal Husbandry and Veterinary Sciences Abdul Wali Khan University Mardan College of Animal Science and Technology Yunnan Agricultural University Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and BiotechnologyCollege of Life SciencesBeijing Normal University US Naval Medical Research Unit-3 (NAMRU 3) ANSES Animal Health Laboratory

Objective: To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis(CL) is endemic and was thought to be caused by Leishmania tropica only. Methods: Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011–2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. Results: Leishmania amastigotes were detected by microscopy in 308 of 432 samples(71.3%) while 374 out of 432 samples(86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed Leishmania tropica in 351 and Leishmania major in 6 biopsy samples. Conclusions: This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of Leishmania major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.

关键词： Cutaneous leishmaniasis ITS-PCR RFLP Leishmania tropica Khyber Pakhtunkhwa

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