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HepG2.2.15-derived exosomes facilitate the activation and fibrosis of hepatic stellate cells

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World Journal of Gastroenterology 2024年第19期30卷 2553-2563页

作者： Yang Gao Li Li Sheng-ning zhang Yuan-Yi Mang Xi-Bing zhang Shi-Ming Feng Department of Hepatobiliary Pancreatic and Vascular Surgery The Affiliated Calmette Hospital of Kunming Medical University and The First Hospital of KunmingKunming 650011Yunnan ProvinceChina

BACKGROUND The role of exosomes derived from HepG2.2.15 cells,which express hepatitis B virus(HBV)-related proteins,in triggering the activation of LX2 liver stellate cells and promoting liver fibrosis and cell proliferation remains *** focus was on comprehending the relationship and influence of differentially expressed microRNAs(DE-miRNAs)within these *** To elucidate the effect of exosomes derived from HepG2.2.15 cells on the activation of hepatic stellate cell(HSC)LX2 and the progression of liver *** Exosomes from HepG2.2.15 cells,which express HBV-related proteins,were isolated from parental HepG2 and WRL68 *** blotting was used to confirm the presence of the exosomal marker protein *** activation of HSCs was assessed using oil red staining,whereas DiI staining facilitated the observation of exosomal uptake by LX2 ***,we evaluated LX2 cell proliferation and fibrosis marker expression using 5-ethynyl-2′-deoxyuracil staining and western blotting,***-miRNAs were analyzed using *** Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways were used to annotate the target genes of *** Exosomes from HepG2.2.15 cells were found to induced activation and enhanced proliferation and fibrosis in LX2 cells.A total of 27 miRNAs were differentially expressed in exosomes from HepG2.2.15 *** analysis indicated that these DE-miRNA target genes were associated with cell differentiation,intracellular signal transduction,negative regulation of apoptosis,extracellular exosomes,and RNA *** pathway analysis highlighted ubiquitin-mediated proteolysis,the MAPK signaling pathway,viral carcinogenesis,and the toll-like receptor signaling pathway,among others,as enriched in these *** These findings suggest that exosomes from HepG2.2.15 cells play a substantial role in the activation,proliferation,and fibrosis of LX2 cells and that DE-miRNAs within these exos

关键词： Hepatic stellate cells Liver fibrosis Exosomes Small RNA sequencing HepG2.2.15

Alternative relay regulates the adenosine triphosphatase activity of Locusta migratoria striated muscle myosin

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Insect Science 2024年第2期31卷 435-447页

作者： Jie Hao Chang Liu ning zhang Jing Li Tong Ni Mingbo Qu Xiang-dong Li State Key Laboratory oflntegrated Management of lnsect Pests and Rodents Insttute of ZoologyChinese Academy of SciencesBejingChina University of Chinese Academy of Sciences BejingChina School of Bioengeering Dalian University of TechnologyDalianChina

Locust(Locusta migratoria)has a single striated muscle myosin heavy chain(Mhc)gene,which contains 5 clusters of alternative exclusive exons and 1 differently included penultimate *** alternative exons of Mhc gene encode 4 distinct regions in the myosin motor domain,that is,the N-terminal SH3-like domain,one lip of the nucleotide-binding pocket,the relay,and the ***,we investigated the role of the alternative regions on the motor function of locust muscle *** Sf9-baculovirus protein expression system,we expressed and purified 5 isoforms of the locust muscle myosin heavy meromyosin(HMM),including the major isoform in the thorax dorsal longitudinal flight muscle(FL1)and 4 isoforms expressed in the abdominal intersegmental muscle(AB1 to AB4).Among these 5 HMMs,FL1-HMM displayed the highest level of actin-activated adenosine triphosphatase(ATPase)activity(hereafter referred as ATPase activity).To identify the alternative region(s)responsible for the elevated ATPase activity of FL1-HMM,we produced a number of chimeras of FL1-HMM and *** with the relay of AB4-HMM(encoded by exon-14c)substantially decreased the ATPase activity of FL1-HMM,and conversely,the relay of FL1-HMM(encoded by exon-14a)enhanced the ATPase activity of *** showed that the exon-14a-encoded residues Gly474 and Asn509 are responsible for the elevated ATPase activity of *** results indicate that the alternative relay encoded by exon-14a/c play a key role in regulating the ATPase activity of FL1-HMM and AB4-HMM.

关键词： alternative splicing ATPase Locusta migratoria molecular motor muscle myosin

Enhanced homing of mesenchymal stem cells for in situ niche remodeling and bone regeneration

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Nano Research 2024年第8期17卷 7449-7460页

作者： Yin Yuan Lijun Chen Jing Yang Sining Zhou Yangxin Fang Qi zhang ning zhang Yifei Li Li Yuan Fan Jia Shuo Ni Charlie Xiang State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesNational Medical Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Department of Biology The David H.Koch Institute for Integrative Cancer Research at MITMassachusetts Institute of TechnologyCambridgeMA 02139USA Research Units of Infectious disease and Microecology Chinese Academy of Medical SciencesHangzhou 310003China Innovative Precision Medicine(IPM)Group Hangzhou 311215China MOE Key Laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province Department of CardiologySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Cancer Immunology and Virology Dana-Farber Cancer InstituteBostonMA 02215USADepartment of ImmunologyHarvard Medical SchoolBoston MA 02115USA Department of Orthopedic Surgery and Shanghai Institute of Microsurgery on Extremities Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiaotong University School of MedicineShanghai 200233China

Mesenchymal stem cells(MSCs)transplantation is a promising strategy for osteoporosis ***,limited sources and poor tissue-homing efficiency limit their clinical *** this study,we isolated a kind of MSCs from women’s menstrual blood(MenSCs)noninvasively and established a novel MSCs bone marrow-targeted delivery system by utilizing waterin-oil-in-water droplet *** were encapsulated withinβ-cyclodextrin-functionalized alginate microcapsules loaded with zoledronates,which has a high affinity for *** this delivery system,MenSCs could be preferentially delivered to the bone marrow tissues via intravenous infusion,and restored bone mass by remodeling the bone marrow niche in situ in ovariectomized mouse ***,scRNA-seq analysis demonstrated that those MenSCs homed to the bone marrow recruited CD4^(+)FOXP3^(+)natural regulatory T(nT_(reg))cells by secreting *** recruited nTreg promoted CD8^(+)T cells to secret Wnt family member 10B(WNT10B),activating the Wnt signaling in osteoblasts and thus promoting bone formation in situ in the bone *** study reveals a promising application of MenSCs in postmenopausal osteoporosis treatment and highlights the clinical value of MenSCs by encouraging women to reserve autologous MenSCs before menopause to prevent and alleviate postmenopausal osteoporosis.

关键词： mesenchymal stem cells microfluidics bone marrow homing stem cell niche postmenopausal osteoporosis

An effective strategy for dendrite free Li metal anodes:Nickel foam decorated with high lattice-matching CoN and CoF_(2) nanosheets for dense deposition

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Nano Research 2024年第5期17卷 4069-4078页

作者： Wenlong Liu Jianzong Man Xiaodong Sun ning zhang Yehong Du Kun Liu Zhongsheng Wen Song Li Juncai Sun Institute of Materials and Technology Dalian Maritime UniversityDalian 116026China Shandong Provinvial Key Laboratory of Chemical Energy Storage and Novel Cell Technology and School of Chemistry and Chemical enginneringLiaocheng UniversityLiaocheng 252000China Department of Public Security Management Liaoning Police CollegeDalian 116036China

The notorious dendrite and infinite volume change seriously restrict the advancement of lithium metal anodes(LMAs),during the long-term process of stripping/***,the nanosheets of metal fluoride(CoF_(2))and metal nitride(CoN)with magnificent lithiophilicity on the nickel(Ni)foam are designed as the“regulator”to uniform the Li plating and build stronger solid electrolyte interface(SEI)layer for dendrite free *** Ni foam offers abundant space to receive deposited Li *** CoN nanosheets can guarantee the fast transfer of electrons,which provides a stable interface of Li^(+)***,the nanosheet structure with lithiophilicity would accelerate the move of Li+and decrease the nucleation barrier,due to the high lattice-matching of Li and ***,the CoF_(2) could increase the content of F(LiF)in the SEI layer,which enhances the strength and avoids the destruction of SEI *** the cooperation of CoN and CoF_(2),the composited anode(Li/NF@CNCF)exhibits ultra-long cycle performance(more than 1200 h)and fantastic structure stability at 1 mA·cm^(-2) with 1 mAh·cm^(-2).Based on the LiFePO_(4) and Li/NF@CNCF,the full cells deliver excellent specifical capacity and steady coulombic *** strategy contributes an effective approach to alleviate the issues of lithium metal anodes in the field of LMAs.

关键词： CoN and CoF_(2) lattice-matching nanosheet lithiophilicity lithium metal anodes

Primary pulmonary meningioma and minute pulmonary meningothelial-like nodules: Rare pulmonary nodular lesions requiring more awareness in clinical practice

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World Journal of Clinical Cases 2024年第11期12卷 1857-1862页

作者： Li-Dan Liu Ke-Xin zhang Hai-ning zhang Yi-Wen Zheng Hong-Tao Xu Department of Pathology The First Hospital and College of Basic Medical Sciences of China Medical UniversityShenyang 110001Liaoning ProvinceChina

In this editorial,we comment on an article by Ruan et al published in a recent issue of the World Journal of Clinical *** meningothelial proliferative lesions,including primary pulmonary meningiomas,minute pulmonary meningothelial-like nodules,and metastatic pulmonary meningiomas are rare pulmonary *** lesions are difficult to differentiate from lung cancers based on clinical and imaging ***,we briefly introduce the clinical,imaging,and pathological characteristics of these lesions and discuss their pathogenesis to strengthen the current understanding of pulmonary meningothelial proliferative lesions in clinical diagnosis and therapy.

关键词： Pulmonary meningothelial proliferation Primary pulmonary meningioma Minute pulmonary meningothelial-like nodule Lung neoplasm Rare pulmonary nodular lesion

Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2024年第4期31卷 1015-1025页

作者： Meng-Qi zhang Lang-Lang Gong Ya-Qin Zhao Yun-Feng Ma Gui-Jun Long Huan Guo Xuan-Zheng Liu J.Joe Hull Youssef Dewer Chao Yang ning-ning zhang Ming He Peng He National Key Laboratory of Green Pesticides Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina Pest Management and Biocontrol Research Unit US Arid Land Agricultural Research CenterUSDA Agricultural Research ServicesMaricopaArizonaUSA Phytotoxicity Research Department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt Guizhou Jifeng Seed Industry Limited Liability Company XingyiGuizhou ProvinceChina Shandong Facility Horticulture Bioengineering Research Center Weifang University of Science and TechnologyWeifangShandong ProvinceChina

Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal *** is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice *** extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice *** bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult *** at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing *** evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 *** study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.

关键词： CRISPR/Cas9 DIPA-CRISPR gene editing tryptophan 2,3-dioxygenase WBPH

Advanced nano drug delivery systems for neuroprotection against ischemic stroke

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Chinese Chemical Letters 2024年第2期35卷 151-162页

作者： Chenchen Xie Jun Liao ning zhang Yuhan Sun Yi Li Liyan Xiong Yingnan zhang Xiaoyan Liu Wei Su Heming Chen Ping Zeng Xiaoqing zhang Ying Lu Tingfang Wang Chuan zhang Shanghai Engineering Research Center of Organ Repair School of MedicineShanghai UniversityShanghai 200444China Department of Pharmaceutical Science School of PharmacyNaval Medical UniversityShanghai 200433China Department of pharmacy the International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200030China School of Pharmacy Shanghai Jiao Tong UniversityShanghai 200040China

Ischemic stroke(IS)represents a significant threat to brain health due to its elevated mortality and disability *** efficacy of small-molecule neuroprotective agents has been impeded by challenges associated with traversing the blood-brain barrier(BBB)and limited ***,advanced nano drug delivery systems hold promise for overcoming these obstacles by facilitating efficient transportation across the BBB and maintaining optimal drug *** review aims to explore advanced neuroprotective nano drug delivery systems as a means of effectively administering neuroprotective agents to the brain using pharmaceutical approaches in the treatment of *** examining these systems,researchers and clinicians can gain valuable insights and innovative concepts,illuminating the potential of advanced neuroprotective nano drug delivery *** these advancements can drive the progress of pioneering and efficacious therapeutic interventions for IS.

关键词： Target Blood-brain barrier Ischemic stroke Nano drug delivery Neuroprotection

A Distributed Computing Algorithm for Electricity Carbon Emission Flow and Carbon Emission Intensity

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Protection and Control of Modern Power Systems 2024年第2期9卷 138-146页

作者： Xingping Wu Wei Yang ning zhang Chunlei Zhou Jinwei Song Chongqing Kang Big Data Center of China Network Chinese Society of Electrical Engineering State Network Big Data Center Department of Electrical Engineering Tsinghua UniversityBeijingChina 不详 Big Data Center State Grid Corporation of ChinaBeijing 100052China Department of Electrical Engi-neering Tsinghua UniversityBeijingChina

The calculation of the indirect carbon emis-sion is essential for power system policy making,carbon market development,and power grid *** em-bedded carbon emissions of the electricity system are commonly calculated by carbon emission flow ***,the calculation procedure is time-consuming,especially for a country with 500-1000 thousand nodes,making it challenging to obtain nationwide carbon emis-sions intensity ***,the calculation procedure requires to gather all the grid data with high classified levels from different power grid companies,which can prevent data sharing and cooperation among different *** paper proposes a distributed computing algorithm for indirect carbon emission that can reduce the time consumption and provide privacy *** core idea is to utilize the sparsity of the nodes’flow matrix of the nationwide grid to partition the computing procedure into parallel sub-procedures exe-cuted in multiple *** flow and structure data of the regional grid are transformed irreversibly for pri-vacy protection,when transmitted between terminals.A 1-master-and-N-slave layout is adopted to verify the *** algorithm is suitable for large grid compa-nies with headquarter and branches in provinces,such as the State Grid Corporation of China.

关键词： Carbon emission flow cooperative computing carbon emission intensity matrix block par-tition power flow tracing parallel computing privacy protection

Switchable shape memory polymer bio-inspired adhesive and its application for unmanned aerial vehicle landing

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Chinese Journal of Aeronautics 2024年第3期37卷 380-390页

作者： Qingsong HE Zefang ZHAO Qiyun ZHONG Siyuan LIU Kai DENG Yongqi LIU ning zhang Zijie ZHAO Fengjiang ZHAN Jianfeng ZHAO Jiangsu Provincial Key Laboratory of Bionic Functional Materials College of Mechanical and Electrical EngineeringNanjing University of Aeronautics and AstronauticsNanjing 210016China Key Laboratory of Advanced Technology for Small and Medium-Sized UAV Ministry of Industry and Information TechnologyUnmanned Aerial Vehicles Research InstituteNanjing University of Aeronautics and AstronauticsNanjing 210016China State Key Laboratory of Mechanics and Control of Aerospace Structures Nanjing University of Aeronautics and AstronauticsNanjing 210016China Functional Morphology and Biomechanics Institute of ZoologyKiel UniversityKiel 24098Germany

Controlled and switchable adhesion is commonly observed in biological *** recent years,many scholars have focused on making switchable bio-inspired ***,making a bio-inspired adhesive with high adhesion performance and excellent dynamic switching properties is still a challenge.A Shape Memory Polymer Bio-inspired Adhesive(SMPBA)was successfully developed,well realizing high adhesion(about 337 kPa),relatively low preload(about90 kPa),high adhesion-to-preload ratio(about 3.74),high switching ratio(about 6.74),and easy detachment,which are attributed to the controlled modulus and contact area by regulating temperature and the Shape Memory Effect(SME).Furthermore,SMPBA exhibits adhesion strength of80–337 kPa on various surfaces(silicon,iron,and aluminum)with different roughness(Ra=0.021–10.280)because of the conformal contact,reflecting outstanding surface *** finite element analysis verifies the bending ability under different temperatures,while the adhesion model analyzes the influence of preload on contact area and ***,an Unmanned Aerial Vehicle(UAV)landing device with SMPBA was designed and manufactured to achieve UAV landing on and detaching from various *** study provides a novel switchable bio-inspired adhesive and UAV landing method.

关键词： Bio-inspired adhesive Shape memory polymers Switchable adhesive Unmanned aerial vehicle Landing