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情景模拟教学模式在耳鼻喉临床见习中的应用研究——以喉梗阻为例

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首都食品与医药 2024年第14期31卷 115-118页

作者：栾宁赵娜郝雪首都医科大学延庆教学医院北京102100

目的探讨情景模拟教学模式在耳鼻喉临床见习中的应用效果。方法选择首都医科大学燕京医学院2020级和2021级乡村医学专业学生50名,随机分为两组(n=25),选取耳鼻喉科最为急危重症疾病喉梗阻为例,进行教学评估。实验组采用情景模拟教学,对... 详细信息

目的探讨情景模拟教学模式在耳鼻喉临床见习中的应用效果。方法选择首都医科大学燕京医学院2020级和2021级乡村医学专业学生50名,随机分为两组(n=25),选取耳鼻喉科最为急危重症疾病喉梗阻为例,进行教学评估。实验组采用情景模拟教学,对照组采用传统的教学模式。观察分析两组的教学效果。结果实验组学生的理论考核、技能操作能力评分均比对照组高,差异有统计学意义(P<0.05);实践考核(病情评估、诊治思维、应急能力、团队配合能力)优于对照组,差异有统计学意义(P<0.05);满意度调查(诊疗能力、组织协调能力、团队合作能力及对教学满意度)均高于对照组,差异有统计学意义(P<0.05)。结论情景模拟教学模式在耳鼻喉临床见习教学中的效果优于传统教学模式,可显著提高教学质量、团队合作能力及学生满意度,值得推广应用。

关键词：情景模拟教学乡医班学生喉梗阻教学效果

LNP-CpG ODN-adjuvanted varicella-zoster virus glycoprotein E induced comparable levels of immunity with Shingrix^(TM) in VZV-primed mice

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Virologica Sinica 2022年第5期37卷 731-739页

作者： ning luan Han Cao Yunfei Wang Kangyang Lin Cunbao Liu Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeKunming 650118China

Latent varicella-zoster virus(VZV)may be reactivated to cause herpes zoster,which affects one in three people during their *** currently available subunit vaccine Shingrix^(TM) is superior to the attenuated vaccine Zostavax®in terms of both safety and efficacy,but the supply of its key adjuvant component QS21 is *** ionizable lipid nanoparticles(LNPs)that were recently approved by the FDA for COVID-19 mRNA vaccines as carriers,and oligodeoxynucleotides containing CpG motifs(CpG ODNs)approved by the FDA for a subunit hepatitis B vaccine as immunostimulators,we developed a LNP vaccine encapsulating VZV-glycoprotein E(gE)and CpG ODN,and compared its immunogenicity with Shingrix^(TM) in C57BL/6J *** results showed that the LNP vaccine induced comparable levels of gE-specific IgG antibodies to Shingrix^(TM) as determined by enzymelinked immunosorbent assay(ELISA).Most importantly,the LNP vaccine induced comparable levels of cellmediated immunity(CMI)that plays decisive roles in the efficacy of zoster vaccines to Shingrix^(TM) in a VZVprimed mouse model that was adopted for preclinical studies of Shingrix^(TM) .Number of IL-2 and IFN-γsecreting splenocytes and proportion of T helper 1(Th1)cytokine-expressing CD4^(+)T cells in LNP-CpG-adjuvanted VZV-gE vaccinated mice were similar to that of Shingrix^(TM) boosted *** of the components in this LNP vaccine can be artificially and economically synthesized in large quantities,indicating the potential of LNP-CpGadjuvanted VZV-gE as a more cost-effective zoster vaccine.

关键词： Varicella zoster virus(VZV) Subunit vaccine Adjuvant Lipid nanoparticle(LNP) CpG oligodeoxynucleotide(CpG ODN) AS01B Humoral immunity Cell-mediated immunity(CMI)

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运脾化痰通窍方提取工艺优化

世界科学技术-中医药现代化 2024年第3期26卷 591-600页

作者：余金凤艾璟栾宁闫明王振中肖伟于桂芳南京中医药大学康缘中药学院南京210023 江苏康缘药业股份有限公司连云港222001 中药制药过程控制与智能制造技术全国重点实验室连云港222001 南京中医药大学药学院南京210023

目的实现运脾化痰通窍方提取工艺优选,并建立组方指纹图谱。方法应用总量统计矩法,对提取液指纹图谱进行宏观表征和相似度分析,以黄芩苷、汉黄芩苷、迷迭香酸、甘草苷、甘草酸、出膏率为关键质量属性,以提取时间、加水倍量、提取次数为... 详细信息

目的实现运脾化痰通窍方提取工艺优选,并建立组方指纹图谱。方法应用总量统计矩法,对提取液指纹图谱进行宏观表征和相似度分析,以黄芩苷、汉黄芩苷、迷迭香酸、甘草苷、甘草酸、出膏率为关键质量属性,以提取时间、加水倍量、提取次数为关键工艺参数,采用AHP-独立性权重法进行综合评分,优选出最佳提取工艺,并进行验证。结果建立总量统计矩相似度评价方法,不同提取方法指纹图谱总量统计矩相似度均值为0.8807,工艺评价模型函数方差分析结果显示,P<0.0001、R2=0.9904,该模型具有统计学意义。优选得到最佳提取工艺为全方饮片加10倍量水,提取1.5 h,提取2次。结论优化的工艺稳定可靠,建立了符合该方提取液指纹图谱特点的总量统计矩分析方法,为该方后续工艺研究全过程质量控制奠定物质基础。

关键词：运脾化痰通窍方提取工艺 Box-Behnken设计-响应面法指纹图谱 AHP-独立性权重法质量控制

Preliminary investigation into feasibility of dissolved methane measurement using cavity ringdown spectroscopy technique

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Frontiers of physics 2016年第6期11卷 159-166页

作者： Zhen-Nan Wang Wang-Quan Ye Xiao-ning luan Fu-Jun Qi Kai Cheng Ronger Zheng Optics and Optoelectronies Laboratory Ocean University of China Qingdao 266100 China

For the exploration of gas hydrate resources by measuring the dissolved methane concentration in seawater, a continuous-wave cavity ringdown spectroscopy (CW-CRDS) experimental setup was constructed for trace methane detection. A current-modulation method, rather than a cavity-modulation method using an optical switch and a piezoelectric transducer, was employed to realize the cavity excitation and shutoff. Such a current-modulation method enabled the improvement of the experimental setup construction and stability, and the system size and stability are critical for a sensor to be deployed underwater. Ringdown data acquisition and processing were performed, followed by an evaluation of the experimental setup stability and sensitivity. The obtained results demonstrate that great errors are introduced when a large fitting window is selected if the analog-to-digital converter has an insufficient resolution. The ringdown spectrum of methane corresponding to the 2 nu(3) band R(4) branch was captured, and the methane concentration in lab air was determined to be 2.06 ppm. Further experiments for evaluating the quantitative ability of this CW-CRDS experimental setup are underway from which a high-sensitivity methane sensor that can be combined with a degassing system is expected.

关键词： gas hydrate methane continuous-wave cavity ringdown spectroscopy current-modulation method

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城轨车辆转向架智能组装生产线设计

智慧轨道交通 2024年第2期61卷 41-46页

作者：祝传钰姜衍猛杨志强栾宁康立飞中车青岛四方车辆研究所有限公司智能装备事业部山东青岛266031

目前转向架生产已呈现出批量化、智能化、信息化的需求,传统的城轨车辆转向架生产组装模式已难以满足。因此,针对传统组装模式存在的布局混乱、依赖人工、存在工位瓶颈、设备落后、自动化智能化水平低、信息管控缺失等不足,文章以“精... 详细信息

目前转向架生产已呈现出批量化、智能化、信息化的需求,传统的城轨车辆转向架生产组装模式已难以满足。因此,针对传统组装模式存在的布局混乱、依赖人工、存在工位瓶颈、设备落后、自动化智能化水平低、信息管控缺失等不足,文章以“精益化”、“智能化”、“信息化”为设计原则,介绍了城轨车辆转向架智能组装生产线的设计理念、工艺流程、作业节拍、方案布局、智能化设备以及信息化管控;从而提升组装质量及作业效率,实现城轨车辆转向架组装作业的智能化升级。

关键词：城轨车辆转向架组装生产线工艺优化智能装备

Antimicrobial peptide LL-37 forms complex with bacterial DNA to facilitate blood translocation of bacterial DNA and aggravate ulcerative colitis

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Science Bulletin 2018年第20期63卷 1364-1375页

作者： Zilei Duan Yaqun Fang Yang Sun ning luan Xue Chen Mengrou Chen Yajun Han Yizhu Yin James Mwangi Junkun Niu Kunhua Wang Yinglei Miao Zhiye Zhang Ren Lai Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province Kunming Institute .of ZoologyKunming 650223China Life Sciences College of Nanjing Agricultural University Nanjing 210095China Department of Gastroenterology The First Affiliated Hospital of Kunming Medical UniversityKunming 650032China Department of General Surgery The First Affiliated Hospital of Kunming Medical UniversityKunming 650032China Kunming College of Life Science University of Chinese Academy of SciencesKunming 650204China Sino-African Joint Research Center Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China

Bacterial DNA(bacDNA) is frequently found in serum of patient with ulcerative colitis(UC) and Crohn's disease, even blood bacterial culture is negative. How bacDNA evades immune elimination and is translocated into blood remain unclear. Here, we showed that bacDNA avoids elimination and disables bacteriakilling function of antimicrobial peptide LL-37(Cramp in mice) by forming complex with LL-37, which is inducible after culture with bacteria or bacterial products. Elevated LL-37-bacDNA complex was found in plasma and lesions of patients with UC. LL-37-bacDNA promoted inflammation by inducing Th1, Th2 and Th17 differentiation and activating toll-like receptor-9(TLR9). The complex also increased paracellular permeability, which possibly combines its inflammatory effects to promote local damage and bacDNA translocation into blood. Cramp-bacDNA aggravated mouse colitis severity while interference with the complex ameliorated the disease. The study identifies that inflammatogenic bacDNA utilizes LL-37 as a vehicle for blood translocation and to evade immune elimination. Additionally, bacteria may make a milieu by releasing bacDNA to utilize and resist host antimicrobial peptides as a ‘trojan horse'.

关键词： Ulcerative colitis Bacterial DNA LL-37 Immune elimination Chronic inflammation

A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing ProteinaseInhibiting Activity

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Natural Products and Bioprospecting 2015年第4期5卷 209-214页

作者： Yu-Wei Wang Ji-Min Tan Can-Wei Du ning luan Xiu-Wen Yan Ren Lai Qiu-Min Lu Life Sciences College of Nanjing Agricultural University Nanjing 210095JiangsuChina Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyKunming 650223YunnanChina

Various bio-active substances in amphibian skins play important roles in survival of the *** protease inhibitor peptides have been identified from amphibian skins,which are supposed to negatively modulate the activity of proteases to avoid premature degradation or release of skin peptides,or to inhibit extracellular proteases produced by invading ***,there is no information on the proteinase inhibitors from the frog Lepidobatrachus laevis which is unique in South *** this work,a cDNA encoding a novel trypsin inhibitor-like(TIL)cysteine-rich peptide was identified from the skin cDNA library of *** 240-bp coding region encodes an 80-amino acid residue precursor protein containing 10 *** sequence comparison and signal peptide prediction,the precursor was predicted to release a 55-amino acid mature peptide with amino acid sequence,*** mature peptide was named ***-TIL shares significant domain similarity with the peptides from the TIL supper *** and trypsin-inhibitory abilities of recombinant LL-TIL were *** LL-TIL showed no antimicrobial activity,while it had trypsin-inhibiting activity with aKi of 16.5178 *** results suggested there was TIL peptide with proteinase-inhibiting activity in the skin of frog *** the best of our knowledge,this is the first report of TIL peptide from frog skin.

关键词： Trypsin inhibitor Cysteine-rich peptide Amphibian Skin

Synergistic anionic/zwitterionic mixed surfactant system with high emulsification efficiency for enhanced oil recovery in low permeability reservoirs

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Petroleum Science 2024年第2期21卷 936-950页

作者： Hai-Rong Wu Rong Tan Shi-Ping Hong Qiong Zhou Bang-Yu Liu Jia-Wei Chang Tian-Fang luan ning Kang Ji-Rui Hou National Key Laboratory of Petroleum Resources and Engineering China University of Petroleum-BeijingBeijing102249China Unconventional Petroleum Research Institute China University of Petroleum-BeijingBeijing102249China Gas Production Plant 5 of PetroChina Changqing Oilfield Company PetroChinaXi'an710000ShaanxiChina College of Petroleum Engineering China University of Petroleum-BeijingBeijing102249China College of Science China University of Petroleum-BeijingBeijing102249China Kazakh-British Technical University Almaty050000Kazakhstan

Emulsification is one of the important mechanisms of surfactant flooding. To improve oil recovery for low permeability reservoirs, a highly efficient emulsification oil flooding system consisting of anionic surfactant sodium alkyl glucosyl hydroxypropyl sulfonate(APGSHS) and zwitterionic surfactant octadecyl betaine(BS-18) is proposed. The performance of APGSHS/BS-18 mixed surfactant system was evaluated in terms of interfacial tension, emulsification capability, emulsion size and distribution, wettability alteration, temperature-resistance and salt-resistance. The emulsification speed was used to evaluate the emulsification ability of surfactant systems, and the results show that mixed surfactant systems can completely emulsify the crude oil into emulsions droplets even under low energy conditions. Meanwhile,the system exhibits good temperature and salt resistance. Finally, the best oil recovery of 25.45% is achieved for low permeability core by the mixed surfactant system with a total concentration of 0.3 wt%while the molar ratio of APGSHS:BS-18 is 4:6. The current study indicates that the anionic/zwitterionic mixed surfactant system can improve the oil flooding efficiency and is potential candidate for application in low permeability reservoirs.

关键词： Anionic/zwitterionic mixed surfactant system Emulsification Synergistic effect Low permeability reservoir Enhanced oil recovery

In vivo antimalarial activity of synthetic hepcidin against Plasmodium berghei in mice

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Chinese Journal of Natural Medicines 2017年第3期15卷 161-167页

作者： FANG Ya-Qun SHEN Chuan-Bin luan ning YAO Hui-Min LONG Chen-Bo LAI Ren YAN Xiu-Wen College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China Key Laboratory of Animal Models and Human Disease Mechanisms Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China University of Chinese Academy of Sciences Beijing 100009China

The present study was designed to investigate the antimalarial activity of synthetic hepcidin and its effect on cytokine secretion in mice infected with Plasmodium *** mice were infected with *** intravenously and treated with hepcidin according to 4-day suppression test and Rane's *** serum levels of interleukins(IL-1β,IL-2,IL-6,IL-10,IL-12p70,and IL-17A),tumor necrosis factor-α(TNF-α),and interferon-γ(IFN-γ) in the experimental mice were determined using a cytometric bead array(CBA)*** survival rate of the infected mice was also ***,the serum iron,alanine transaminase(ALT),aspartate transaminase(AST),and total bilirubin(BIL) were detected to evaluate liver *** exerted direct anti-malarial function in vivo and increased survival rate in a dose-dependent *** addition,the secretion of T helper cell type 1(Th1),Th2,and Th17 cytokines,TNF-α,and IFN-γ were inhibited by *** conclusion,our results demonstrated that synthetic hepcidin exerts in vivo antimalarial activity and possesses anti-inflammatory function,which provides a basis for future design of new derivatives with ideal anti-malarial activity.

关键词： Hepcidin Malaria Cytokine Inflammation Iron