A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing ProteinaseInhibiting Activity
作者机构：Life Sciences College of Nanjing Agricultural UniversityNanjing 210095JiangsuChina Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences&Yunnan ProvinceKunming Institute of ZoologyKunming 650223YunnanChina
出 版 物：《Natural Products and Bioprospecting》 (应用天然产物（英文）)
年 卷 期：2015年第5卷第4期
页 面：209-214页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 070303[理学-有机化学] 0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学]
基 金：the China National Natural Science Foundation of Young Scientists(No.31201717) Special Foundation for Young Scientists of Jiangsu Province(No.BK2012365) Jiangsu Province Science and Technology Support Project(No.BE2012748)
主 题：Trypsin inhibitor Cysteine-rich peptide Amphibian Skin
摘 要：Various bio-active substances in amphibian skins play important roles in survival of the *** protease inhibitor peptides have been identified from amphibian skins,which are supposed to negatively modulate the activity of proteases to avoid premature degradation or release of skin peptides,or to inhibit extracellular proteases produced by invading ***,there is no information on the proteinase inhibitors from the frog Lepidobatrachus laevis which is unique in South *** this work,a cDNA encoding a novel trypsin inhibitor-like(TIL)cysteine-rich peptide was identified from the skin cDNA library of *** 240-bp coding region encodes an 80-amino acid residue precursor protein containing 10 *** sequence comparison and signal peptide prediction,the precursor was predicted to release a 55-amino acid mature peptide with amino acid sequence,*** mature peptide was named ***-TIL shares significant domain similarity with the peptides from the TIL supper *** and trypsin-inhibitory abilities of recombinant LL-TIL were *** LL-TIL showed no antimicrobial activity,while it had trypsin-inhibiting activity with aKi of 16.5178 *** results suggested there was TIL peptide with proteinase-inhibiting activity in the skin of frog *** the best of our knowledge,this is the first report of TIL peptide from frog skin.
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