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西藏桑桑牦牛CXCR2基因克隆及生物信息学分析

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中国草食动物科学 2024年第5期44卷 57-63页

作者：申金伟洛桑顿珠平措占堆卓玛次仁尼玛加措成述儒梁春年中国农业科学院兰州畜牧与兽药研究所甘肃省牦牛繁育工程重点实验室兰州730050 甘肃农业大学动物科学技术学院兰州730050 西藏自治区农牧科学院畜牧兽医研究所拉萨850004 日喀则市昂仁县农业农村局昂仁857001 农业农村部青藏高原畜禽遗传育种重点实验室兰州730050

为了研究牦牛CXCR2基因的结构、生物学功能及影响牦牛生长发育的机制,以西藏桑桑牦牛脂肪组织cDNA为模板,利用RT-PCR克隆牦牛CXCR2基因的编码区序列(Coding sequence,CDS),并对其进行生物信息学分析、结构域预测及蛋白理化性质预测,利用... 详细信息

为了研究牦牛CXCR2基因的结构、生物学功能及影响牦牛生长发育的机制,以西藏桑桑牦牛脂肪组织cDNA为模板,利用RT-PCR克隆牦牛CXCR2基因的编码区序列(Coding sequence,CDS),并对其进行生物信息学分析、结构域预测及蛋白理化性质预测,利用RT-qPCR技术进行组织表达分析。结果表明,桑桑牦牛CXCR2基因的CDS区全长876 bp,共编码291个氨基酸;结构域预测结果显示,在CXCR2氨基酸序列的1~245位有1个Pfam家族的7tm_1结构域;CXCR2蛋白分析预测结果显示,该蛋白有5个跨膜结构,无信号肽区域,分子量32855.55 Da,原子总数4726个,分子式为C_(1524)H_(2416)N_(392)O_(374)S_(20),不稳定性指数29.45,表明该蛋白质为稳定蛋白,理论等电点9.86,脂肪系数120.27,总平均亲水系数-0.657,为疏水性蛋白,共有21个磷酸化位点;亚细胞定位预测结果显示,桑桑牦牛CXCR2基因主要分布于内质网(55.6%)、线粒体(22.2%)和细胞核(22.2%)中;系统进化树结果显示,桑桑牦牛与瘤牛亲缘关系最近,与水牛亲缘关系最远。

关键词：桑桑牦牛 CXCR2基因克隆生物信息学分析

Kinetics of zinc sulfide concentrate direct leaching in pilot plant scale and development of semi-empirical model

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Transactions of Nonferrous Metals Society of China 2017年第10期27卷 2272-2281页

作者： nima SADEGHI Javad MOGHADDAM Mehdi OJAGHI ILKHCHI Faculty of Materials Engineering Sahand University of Technology Tabriz Iran Materials and Metallurgical Department University of Zanjan Zanjan Iran

The direct leaching kinetics of an iron-poor zinc sulfide concentrate in the tubular reactor was *** tests werecarried out in the pilot *** allow the execution of hydrostatic pressure condition,the slurry with ferrous sulfate and sulfuric acidsolution was filled into a vertical tube(9m in height)and air was blown from the bottom of the *** effects of initial acidconcentration,temperature,particle size,initial zinc sulfate concentration,pulp density and the concentration of Fe on the leachingkinetics were *** of the kinetic analysis indicate that direct leaching of zinc sulfide concentrate follows shrinkingcore model(SCM).This process was controlled by a chemical reaction with the apparent activation energy of49.7kJ/***,a semi-empirical equation is obtained,showing that the order of the iron,sulfuric acid and zinc sulfate concentrationsand particle radius are0.982,0.189,-0.097and-0.992,*** of the unreacted and reacted sulfide particles bySEM-EDS shows that insensitive agitation in the reactor causes detachment of the sulfur layer from the particles surface in lowerthan60%Zn conversion and lixiviant in the face with sphalerite particles.

关键词： kinetics direct leaching sphalerite shrinking core model (SCM) pilot plant

Optimization of synchronized frequency and voltage control for a distributed generation system using the Black Widow Optimization algorithm

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Clean Energy 2022年第1期6卷 105-118页

作者： Baran Sadeghi nima Shafaghatian Reza Alayi Mamdouh El Haj Assad Farhad Zishan Hasan Hosseinzadeh Electrical Engineering Department Ghazvin BranchIslamic Azad UniversityGhazvinIran Electrical Engineering Department Zanjan UniversityZanjanIran Department of Mechanics Germi BranchIslamic Azad UniversityGermiIran Sustainable and Renewable Energy Engineering Department University of SharjahP O Box 27272SharjahUAE Department of Electrical Engineering Sahand University of TechnologyTabrizIran Department of Mathematics Ardabil BranchIslamic Azad UniversityArdabilIran

A distributed generation network could be a hybrid power system that includes wind-diesel power generation based on induction generators(IGs)and synchronous generators(SGs).The main advantage of these systems is the possibility of using renewable energy in their *** most important challenge is to design the voltage-control loop with the frequency-control loop to obtain optimal responses for voltage and frequency *** this work,the voltage-control loop is designed by an automatic voltage regulator.A linear model of the hybrid system has also been developed with coordinated voltage and frequency *** frequency response and voltage deviations are compared for different load disturbances and different reactive *** gains of the SG and the static volt-ampere reactive compensator(SVC)controllers in the IG terminal are calculated using the Black Widow Optimization(BWO)algorithm to insure low frequency and voltage *** BWO optimization algorithm is one of the newest and most powerful optimization methods to have been introduced so *** results showed that the BWO algorithm has a good speed in solving the proposed objective function.A 22%improvement in time adjustment was observed in the use of an optimal ***,an 18%improvement was observed in the transitory values.

关键词： microgrid voltage and frequency control Black Widow Optimization algorithm dynamic response smart grid

Comparison of accuracy and computational performance between the machine learning algorithms for rate of penetration in directional drilling well

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Petroleum Research 2021年第3期6卷 271-282页

作者： Omid Hazbeh Saeed Khezerloo-ye Aghdam Hamzeh Ghorbani nima Mohamadian Mehdi Ahmadi Alvar Jamshid Moghadasi Faculty of Earth Sciences Shahid Chamran UniversityAhwazIran Department of Petroleum Engineering Amirkabir University of TechnologyTehranIran Young Researchers and Elite Club Ahvaz BranchIslamic Azad UniversityAhvazIran Young Researchers and Elite Club Omidiyeh BranchIslamic Azad UniversityOmidiyehIran Faculty of Engineering Department of Computer EngineeringShahid Chamran UniversityAhwazIran Petroleum Engineering Department Petroleum Industry UniversityAhvazIran

Oil and gas reservoirs are of the main assets of countries possessing *** from these reservoirs is one of the main concerns of engineers,which can be achieved by drilling oil and gas *** of hydrocarbon wells is one of the most expensive operations in the oil *** of the most important parameters affecting drilling cost is the rate of penetration(ROP).This paper predicts the rate of penetration using artificial intelligence and hybrid models on Kaboud oil field well#7 in the directional *** this study,different models were constructed through various approaches based on 1878 dataset obtained from drilling operation in the well#***,the accuracy of the constructed models was compared with each *** was found that the MLP-ABC algorithm predicts the rate of penetration more accurately,by far,as compared with other *** MLP-ABC algorithm achieves impressive ROP prediction accuracy(RMSE=0.007211 m/h;AAPD=0.1871%;R^(2)=1.000 for the testing subset).Consequently,it can be concluded that this method is applicable to predict the drilling rate in that oilfield.

关键词： Rate of penetration Artificial intelligence Directional drilling MLP Prediction

Investigating the inhibition of NMDA glutamate receptors in the basolateral nucleus of the amygdala on the pain and inflammation induced by formalin in male Wistar rats

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Frontiers in Biology 2018年第2期13卷 149-155页

作者： nima Heidary Hedayat Sahraei Mohammad Reza Afarinesh Zahra Bahari Gholam Hossein Meftahi Department of Physiology School of Medicine Zanjan University of Medical Sciences Zanjan Iran Neuroscience Research Center Baqiyatallah University of Medical Sciences Tehran Iran Kerman Cognitive Research Center and Neuroscience Research Center Institute of Neuropharmachology Kerman University of MedicalSciences Kerman Iran Department of Physiology and Biophysics Faculty of Medicine Baqiyatallah University of Medical Sciences Tehran Iran

BACKGROUND： The role of the amygdala in controlling emotional pain has been emphasized in several studies. In this study, the role of the NMDA glutamate receptors in the basolateral nucleus of the amygdala （BLA） in regulating inflammation and emotional pain, induced by formalin, was studied in male rats. METHODS： Male Wistar rats, weighing 250±0 g, were injected with 20 μL of 2% formalin into the paw of the right hind limb. Memantine, at doses of 1 and 5 μg/rat, was injected bilaterally into the BLA five minutes prior to injecting formalin. Following the injection, the pain and inflammation of the paws were measured using Dubbison-Dennis and mercury immersion methods, respectively. The behavior of the animals, including licking time and foot volume, was assessed. RESULTS： The results showed that the inactivation of the NMDA receptors in the BLA in the acute phase of pain reduced the licking time （the emotional aspect of pain）. However, at a high dose （5μg/rat）, memantine exacerbates the pain induced by formalin in the chronic phase. Additionally, the inhibition of the NMDA receptors in the BLA by memantine enhanced the formalin-induced increase in foot volume （inflammation） in a dose-dependent manner. CONCLUSION： The study showed that the NMDA glutamate receptors in the BLA are crucial for the emotional pain and inflammation in both chronic and acute phases of formalin-induced pain. However, their roles are more pronounced in the chronic phase than in the acute phase of pain.

关键词： basolateral nucleus of amygdala formalin test inflammation licking time memantine

西藏芫根中总多糖含量测定方法及单糖指纹图谱研究

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中国药业 2024年第7期33卷 83-87页

作者：吕倩倩谢和兵尼玛次仁白玛旦增安徽中医药大学安徽合肥230013 江苏省南通市海门长三角药物高等研究院江苏南通226133 江苏神猴医药研究有限公司江苏南通226133 西藏神猴药业有限责任公司西藏日喀则857000

目的建立测定西藏芫根中总多糖含量的紫外光谱法,以及单糖的高效液相色谱(HPLC)指纹图谱。方法采用紫外分光光度法,以D-无水葡萄糖为对照品,以5%苯酚-硫酸为显色剂,于484 nm波长处测定6批样品中总多糖含量。采用柱前衍生HPLC法建立单糖... 详细信息

目的建立测定西藏芫根中总多糖含量的紫外光谱法,以及单糖的高效液相色谱(HPLC)指纹图谱。方法采用紫外分光光度法,以D-无水葡萄糖为对照品,以5%苯酚-硫酸为显色剂,于484 nm波长处测定6批样品中总多糖含量。采用柱前衍生HPLC法建立单糖的指纹图谱,色谱柱为Ultimate XB-C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-pH为6.8的0.1%磷酸盐溶液(梯度洗脱),流速为1.0 mL/min,检测波长为250 nm,柱温为30℃,进样量为10μL。采用中药色谱指纹图谱相似度评价系统(2012.0版),建立6批芫根药材单糖的HPLC指纹图谱并进行相似度评价,与对照品比对进行共有峰指认。结果D-无水葡萄糖质量浓度在1.84~18.37 mg/L范围内与吸光度线性关系良好(r=0.9995,n=6),精密度、重复性、稳定性试验结果的RSD均小于2.0%;平均加样回收率为100.88%,RSD为1.00(n=6)。6批样品中总多糖的平均含量为285.48 mg/g。单糖HPLC指纹图谱共识别出19个共有峰,确定峰14为半乳糖、峰4为D-甘露糖、峰7为鼠李糖、峰16为阿拉伯糖、峰13为D-无水葡萄糖、峰10为D-葡萄糖醛酸、峰12为D-半乳糖醛酸;相似度为0.946~1.000。结论该方法操作简便、结果稳定可靠,可用于西藏芫根中总多糖的质量控制与评价。

关键词：芫根总多糖紫外分光光度法高效液相色谱法指纹图谱

Comment on “Chen et al., Fabrication and photovoltaic conversion enhancement...”,Electrochimica Acta,2014

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Journal of Semiconductors 2015年第9期36卷 97-98页

作者： Lara Valentic nima E.Gorji Department of Environment Sciences,University of Nova Gorica Department of Physics,Brown University Department of Physics Brown University Providence 02912 Rhode Island USA Department of Environment Sciences University of Nova Gorica Nova Gorica 5000 Slovenia

In a recent article, Chen et aL [Electrochimica Acta, 2014, 130： 279] presented their fabrication and characterization results on a graphene/n-Si solar cell where the Au nanoparticles were inserted in graphene to increase its optical and electrical properties. The higher efficiency of the device was attributed to increased conductivity of graphene after doping with Au nanoparticles. However, the knowledge in the field of Schottky diode solar cells relates this to increased band bending at the junction. Also, to explain the instability behaviour, they concluded that the growth of silicon oxide on the Si surface or oxygen adsorption on the window layer resulted in the device performance increasing initially and decreasing in the end. However, this instability seems to be due to variation in series resistance reduced at the beginning because of slightly lowered Fermi level and increased at the end by the self-compensation by deep in-diffusion ofAu nanoparticles into n-Si layer. We also propose that inserting a very thin p-type layer at the junction will enhance the carrier collection and performance of this device.

关键词： Shockley barrier solar cell graphene Si Au nanoparticle

西藏地区膀胱尿路上皮癌患者GATA-3和H3K27me3的表达及其临床意义

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协和医学杂志 2024年第4期15卷 953-959页

作者：尼玛卓玛肖雨罗含欢多布啦王寒达珍斯朗江村郭平平廖瑞倩西藏自治区人民医院病理科拉萨850000 中国医学科学院北京协和医院病理科北京100730 西藏自治区人民医院泌尿外科拉萨850000

目的探讨西藏地区膀胱尿路上皮癌(bladder urothelial carcinoma,BUC)患者膀胱组织中GATA-3和H3K27me3的表达及其临床意义。方法回顾性收集西藏自治区人民医院2016年1月—2021年12月间行膀胱癌手术的BUC患者的临床资料及其病理组织,以... 详细信息

目的探讨西藏地区膀胱尿路上皮癌(bladder urothelial carcinoma,BUC)患者膀胱组织中GATA-3和H3K27me3的表达及其临床意义。方法回顾性收集西藏自治区人民医院2016年1月—2021年12月间行膀胱癌手术的BUC患者的临床资料及其病理组织,以经尿道膀胱黏膜活检术获取的正常膀胱组织作为对照,采用免疫组化染色方法检测BUC和正常膀胱组织中GATA-3和H3K27me3的表达水平,同时结合临床病理特征分析GATA-3和H3K27me3在藏族BUC患者中的表达差异。结果共入选BUC患者70例,其中男性51例,女性19例,平均年龄(60.5±12.0)岁,收集同期正常膀胱组织标本20份,均来自于藏族患者。免疫组化检测结果显示,GATA-3在BUC及正常膀胱组织中高表达率分别为70.0%(49/70)和100%(20/20),GATA-3高表达与男性、病理分级低、组织非浸润相关(P均<0.05);H3K27me3在BUC及正常膀胱组织中高表达率分别为45.7%(32/70)和20.0%(4/20),H3K27me3高表达仅与男性相关(P=0.011)。结论GATA-3在西藏地区BUC患者膀胱组织中表达下调,且随着肿瘤级别的升高表达显著下调,提示GATA-3可能参与BUC发生、发展并与其恶性程度相关,为临床诊疗及判断疾病预后提供了参考。H3K27me3在西藏地区BUC患者膀胱组织中表达上调,提示H3K27me3有望成为诊断BUC的新型免疫标志物。

关键词：西藏地区膀胱尿路上皮癌临床病理特征 GATA-3 H3K27me3

Suppression of human papillomavirus type 16 E5 oncoprotein:A promising step in fostering the treatment of cervical cancer

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Oncology Research 2021年第2期29卷 141-148页

作者： nima HEMMAT MOHAMMAD AMIN DOUSTVANDI ZAHRA ASADZADEH AHAD MOKHTARZADEH BEHZAD BARADARAN HOSSEIN BANNAZADEH BAGHI Immunology Research Center Tabriz University of Medical SciencesTabrizIran Infectious and Tropical Diseases Research Center Tabriz University of Medical SciencesTabrizIran Department of Virology Faculty of MedicineTabriz University of Medical SciencesTabrizIran Department of Immunology Faculty of MedicineTabriz University of Medical SciencesTabrizIran

Cervical cancer is a growing global disease in developing *** infection with human papillomaviruses(HPV)is an essential causative agent in this type of *** studies demonstrate HPV E5 oncoprotein can impress the normal life cycle of HPV-infected cells by targeting some pivotal cellular signaling pathways,such as the epidermal growth factor receptor(EGFR)signaling *** this study,we used E5-siRNA to knockdown that essential oncogene and considered the effect of E5 silencing on proliferation,apoptosis,cell cycle,apoptosis-related gene expression,and the initiator of the EGFR signaling pathway in cervical cancer *** results demonstrate that E5 plays an essential role in the proliferation and inhibited apoptosis in cervical ***,silencing E5 reduces proliferation,increases apoptosis,and elevates related-genes expression of these malignant ***,E5 suppression may be appropriate for ameliorating cervical cancer progression.

关键词： Human papillomavirus e5 transforming protein epidermal growth factor receptor cervical cancer CaSki