Investigating the inhibition of NMDA glutamate receptors in the basolateral nucleus of the amygdala on the pain and inflammation induced by formalin in male Wistar rats

作     者：Nima Heidary Hedayat Sahraei Mohammad Reza Afarinesh Zahra Bahari Gholam Hossein Meftahi 

作者机构：Department of Physiology School of Medicine Zanjan University of Medical Sciences Zanjan Iran Neuroscience Research Center Baqiyatallah University of Medical Sciences Tehran Iran Kerman Cognitive Research Center and Neuroscience Research Center Institute of Neuropharmachology Kerman University of MedicalSciences Kerman Iran Department of Physiology and Biophysics Faculty of Medicine Baqiyatallah University of Medical Sciences Tehran Iran 

出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)

年 卷 期：2018年第13卷第2期

页      面：149-155页

核心收录：

学科分类：071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 09[农学] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0906[农学-兽医学] 0836[工学-生物工程] 0713[理学-生态学] 

基　　金：the Neuroscience Sciences Research Center at Baqiyatallah University of Medical Sciences  Tehran  Iran 

主　　题：basolateral nucleus of amygdala formalin test inflammation licking time memantine 

摘      要：BACKGROUND： The role of the amygdala in controlling emotional pain has been emphasized in several studies. In this study, the role of the NMDA glutamate receptors in the basolateral nucleus of the amygdala （BLA） in regulating inflammation and emotional pain, induced by formalin, was studied in male rats. METHODS： Male Wistar rats, weighing 250±0 g, were injected with 20 μL of 2% formalin into the paw of the right hind limb. Memantine, at doses of 1 and 5 μg/rat, was injected bilaterally into the BLA five minutes prior to injecting formalin. Following the injection, the pain and inflammation of the paws were measured using Dubbison-Dennis and mercury immersion methods, respectively. The behavior of the animals, including licking time and foot volume, was assessed. RESULTS： The results showed that the inactivation of the NMDA receptors in the BLA in the acute phase of pain reduced the licking time （the emotional aspect of pain）. However, at a high dose （5μg/rat）, memantine exacerbates the pain induced by formalin in the chronic phase. Additionally, the inhibition of the NMDA receptors in the BLA by memantine enhanced the formalin-induced increase in foot volume （inflammation） in a dose-dependent manner. CONCLUSION： The study showed that the NMDA glutamate receptors in the BLA are crucial for the emotional pain and inflammation in both chronic and acute phases of formalin-induced pain. However, their roles are more pronounced in the chronic phase than in the acute phase of pain.