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Isolation,characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil

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Frontiers of Agricultural Science and Engineering 2024年第3期11卷 499-513页

作者： Zakiah MUSTAPHA Radziah OTHMAn nik nurnaeimah nik MUHAMMAD nASIR Dhiya Dalila ZAWAWI Mohd Khairi CHE LAH Hafizan JUAHIR School of Agriculture Science and Biotechnology Faculty of Bioresources and Food IndustryUniversiti Sultan Zainal AbidinBesut Campus22200 BesutTerengganuMalaysia Department of Land Management Faculty of AgricultureUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Faculty of Plantation and Agrotechnology Universiti Technologi MARA Cawangan Pahang26400 JengkaPahangMalaysia

This study has isolated,characterized,and identified potential plant growthpromoting rhizobacteria(PGPR)with multiple PGP characteristics(n_(2)-fixation,P-and K-solubilization,IAA,and siderophores production)from the rhizosphere BRIS soil of Acacia mangium.A total of 24 pure colonies were isolated and only 8 colonies were selected for further evaluation of the growth rate in 5%organic molasses medium supplemented with 2%KnO_(3).Based on the biochemical,potential PGP characteristics and growth performance,3 superior PGPR strains were selected and identified as Paraburkholderia unamae(UA1),Bacillus amyloliquefaciens(UA6),and Enterobacter asburiae(UAA2)by partial sequencing of the 16S rRnA *** selected bacterial strains either in single or mixed(UA1+UA6+􀀁UAA2)cultures have shown a significant biochemical estimation of the PGP *** strain has its own PGPR traits superiority with UA1 showing the best PGP characteristic followed by UA6 and *** use of mixed bacterial strains was beneficial as it showed the best performance in n_(2)-fixation,siderophores production,and significant effect on corn phenology,growth and yield compared to using a single *** types of microbes showed potential to be used as biofertilizer and should be exploited more.

关键词： Biofertilizer corn organic molasses medium PGP characteristic phytohormone potassium nitrate siderophores

numerical solutions for cracks in an elastic half-plane

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Acta Mechanica Sinica 2019年第1期35卷 212-227,I0006页

作者： n.R.F.Elfakhakhre n.M.A.nik Long Z.K.Eshkuvatov Mathematics Department Faculty of ScienceUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Institute for Mathematical Research Universiti Putra Malaysia43400 SerdangSelangorMalaysia Faculty of Science and Technology Universiti Sains Islam Malaysia71800 NilaiNegeri SembilanMalaysia

The behavior of the stress intensity factor at the tips of cracks subjected to uniaxial tension σχ^∞= p with traction-free boundary condition in half-plane elasticity is investigated. The problem is formulated into singular integral equations with the distribution dislocation function as unknown. In the formulation, we make used of a modified complex potential. Based on the appropriate quadrature formulas together with a suitable choice of collocation points, the singular integral equations are reduced to a system of linear equations for the unknown coefficients. numerical examples show that the values of the stress intensity factor are influenced by the distance from the cracks to the boundary of the half-plane and the configuration of the cracks.

关键词： Elastic half-plane Multiple cracks Singular integral equation Sine-shaped crack

Stress Intensity Factor for the Interaction between a Straight Crack and a Curved Crack in Plane Elasticity

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Acta Mechanica Solida Sinica 2016年第4期29卷 407-415页

作者： M.R.Aridi n.M.A.nik Long Z.K.Eshkuvatov Institute for Mathematical Research Universiti Putra Malaysia College of Foundation and General Studies Universiti Tenaga Nasional Mathematics Department Faculty of Science Universiti Putra Malaysia

Formulation in terms of hypersingular integral equations for the interaction between straight and curved cracks in plane elasticity is obtained using the complex variable functions method. The curved length coordinate method and a suitable numerical scheme are used to solve such integrals numerically for the unknown function, which are later used to find the stress intensity factor, SIF.

关键词： hypersingular integral equation complex variable function method curved length coordinate method stress intensity factor

Sicilian serpentinite xenoliths containing abiotic organics with nanodiamond clusters as key model for prebiotic processes

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Geoscience Frontiers 2021年第1期12卷 255-263页

作者： Sergei K.Simakov Vittorio Scribano nikolai n.Mel’nik Germana Barone LLC“ADAMAnT”Skolkovo Participant Tikhoretsky 10/1-61St.Petersburg194064Russia Department of Biological Geological and Environmental SciencesUniversity of CataniaCorso Italia 55-I-95129 CataniaItaly Lebedev Physics Institute Russian Academy of SciencesLeninsky Pr.53Moscow119991Russia

Rock fragments from the deepest parts of a buried hydrothermal system belonging to the Mesozoic Tethys Ocean entered as xenoliths in a Miocenic diatreme,hence brought to the surface,in the Hyblean Plateau(Sicily).Some xenoliths consist of strongly serpentinized ultramafic rocks bearing blebs of abiotic organic matter,where clusters of amorphous carbon nanoparticles,including nanodiamonds,are *** an occurrence conjures up established hypotheses that diamond surfaces are suitable catalytic platforms stimulating the assemblage of complex bio-organic molecules relevant to the emergence of life on *** appearance of bio-organic molecules under primitive Earth conditions is one of the major unsolved questions on the origin of *** we report new micro-Raman spectra on blebs of abiotic organic matter from a selected *** bands were related to hydrogenated nanocrystalline diamonds,with size of nearly 1-1.6 nm,formed from organics at low pressures and *** particular,diamond surfaces can give rise to crystalline interfacial water layers that may have played a fundamental role in the early biosphere evolution as a good medium for rapidly transporting positive charges in the form of hydrated ***,proton gradients in alkaline hydrothermal vents along oceanic ridges are generally viewed as key pre-biotic *** general,serpentinites span the entire geological record,including prebiotic *** hydrous ultramafic rocks often display evidence of abiotic carbon species,both organic and inorganic,including nanodiamonds,being also capable to give rise to chemiosmotic processes and proton gradients necessary to the organisms,such as the"Last Universal Common Ancestor"(LUCA),in the prebiotic Earth.

关键词： Hydrothermal systems nanocarbons Sulfides Fr-t synthesis Abiogenesis LUCA,Sicily Micro-Raman

nUMERICAL SOLUTIOnS FOR A nEARLY CIRCULAR CRACK WITH DEVELOPInG CUSPS UnDER SHEAR LOADInG

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Acta Mechanica Solida Sinica 2011年第5期24卷 439-449页

作者： n.M.A.nik Long L.F.Koo Z.K.Eshkuvatov Department of Mathematics Faculty of ScienceUniversity Putra Malaysia Institute for Mathematical Research University Putra Malaysia

In this paper, we study the behavior of the solution at the crack edges for a nearly circular crack with developing cusps subject to shear loading. The problem of finding the resulting force can be written in the form of a hypersingular integral equation. The equation is then trans-formed into a similar equation over a circular region using conformal mapping. The equation is solved numerically for the unknown coefficients, which will later be used in finding the stress intensity factors. The sliding and tearing mode stress intensity factors are evaluated for cracks and displayed graphically. Our results seem to agree with the existing asymptotic solution.

关键词： nearly circular crack numerical method Galerkin method stress intensity factors hypersingular integral equation shear loading conformal mapping

Materials information and mechanical response of TRIP/TWIP Ti alloys

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npj Computational Materials 2021年第1期7卷 817-825页

作者： Guohua Zhao Xiaoqing Li nik Petrinic Department of Engineering Science University of OxfordOxfordUK Department of Materials Science and Engineering KTH Royal Institute of TechnologyStockholmSweden

Materials innovation calls for an integrated framework combining physics-based modelling and data-driven informatics.A dislocation-based constitutive model accounting for both transformation-induced plasticity(TRIP)and twinning-induced plasticity(TWIP)was built to interpret the mechanical characteristics of metastable titanium *** attention was placed on quantitatively understanding the composition-sensitive phase stability and its influence on the underlying deformation *** this purpose,a pseudoelastic force balance incorporating thermodynamics and micromechanics was applied to calculate the energy landscapes ofβ→α″martensitic transformation,{332}(113)twinning and dislocation *** material data were probed,computed and fed to the *** results revealed that TRIP and TWIP may operate simultaneously because of the presence of a noticeably overlapped energy domain,and confirmed{332}(113)twinning is an energetically favourable deformation *** model validation further unveiled that the activation ofβ→α″transition remarkably enhances the strain-hardening and plasticity,even though the dynamically formedα″volume fraction is much less than that of deformation *** work suggests that the synchronised physical metallurgy and data-driven strategy allows to identify the compositional scenarios for developing high-performance engineering alloys.

关键词： deformation alloys dislocation

Identification of α-glucosidase inhibitors from Clinacanthus nutans leaf extract using liquid chromatography-mass spectrometry-based metabolomics and protein-ligand interaction with molecular docking

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Journal of Pharmaceutical Analysis 2019年第2期9卷 91-99页

作者： Suganya Murugesu Zalikha Ibrahim Qamar Uddin Ahmed Bisha Fathamah Uzir nik Idris nik Yusoff Vikneswari Perumal Faridah Abas Khozirah Shaari Alfi Khatib Department of Pharmaceutical Chemistry Kulliyyah of PharmacyInternational Islamic University Malaysia Department of Pharmaceutical Technology Faculty Pharmacy & Health SciencesUniversiti Kuala LumpurRoyal College of Medicine Perak Laboratory of natural Products Institute of BioscienceUniversiti Putra Malaysia

The present study used in vitro and in silico techniques, as well as the metabolomics approach to characterise α-glucosidase inhibitors from different fractions of Clinacanthus nutans. C. nutans is a medicinal plant belonging to the Acanthaceae family, and is traditionally used to treat diabetes in Malaysia. nHexane, n-hexane: ethyl acetate(1:1, v/v), ethyl acetate, ethyl acetate: methanol(1:1, v/v), and methanol fractions were obtained via partitioning of the 80% methanolic crude extract. The in vitro α-glucosidase inhibitory activity was analyzed using all the fractions collected, followed by profiling of the metabolites using liquid chromatography combined with mass spectrometry. The partial least square(PLS) statistical model was developed using the SIMCA P^+14.0 software and the following four inhibitors were obtained:(1) 4,6,8-Megastigmatrien-3-one;(2) n-Isobutyl-2-nonen-6,8-diynamide;(3) 1′,2′-bis(acetyloxy)-3′,4′-didehydro-2′-hydro-β, ψ-carotene; and(4) 22-acetate-3-hydroxy-21-(6-methyl-2,4-octadienoate)-olean-12-en-28-oic acid. The in silico study performed via molecular docking with the crystal structure of yeast isomaltase(PDB code: 3 A4 A) involved a hydrogen bond and some hydrophobic interactions between the inhibitors and protein. The residues that interacted include ASn259, HID295, LYS156, ARG335,and GLY209 with a hydrogen bond, while TRP15, TYR158, VAL232, HIE280, ALA292, PRO312, LEU313,VAL313, PHE314, ARG315, TYR316, VAL319, and TRP343 with other forms of bonding.

关键词： Clinacanthus nutans LC-MS-QTOF Metabolomics α-Glucosidase inhibitors Diabetes Molecular docking

Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scaffold fabricated by 3D printing

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Bio-Design and Manufacturing 2020年第4期3卷 396-409页

作者： Qiongxi Pan Chenyuan Gao Yingying Wang Yili Wang Cong Mao Quan Wang Sophia n.Economidou Dennis Douroumis Feng Wen lay Poh Tan Huaqiong Li School of Biomedical Engineering School of Ophthalmology and Optometry and Eye HospitalWenzhou Medical UniversityWenzhou 325035Zhejiang ProvincePeople’s Republic of China Engineering Research Center of Clinical Functional Materials and Diagnosis and Treatment Devices of Zhejiang Province Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325011Zhejiang ProvincePeople’s Republic of China Key Laboratory of Orthopedics of Zhejiang Province Department of OrthopedicsThe Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou 325027Zhejiang ProvincePeople’s Republic of China Medway School of Pharmacy University of KentMedway CampusCentral AvenueChatham MaritimeChathamKent ME44TBUK CIPER Centre for Innovation and Process Engineering ResearchKent ME44TBUK School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore 637459Singapore School of Materials Science and Engineering Nanyang Technological UniversitySingapore 639798Singapore

Bone is known to have a natural function to heal ***,if the bone damage is beyond a critical degree,intervention such as bone grafting may be *** this work,the fabrication of a novel bone scaffold composed of natural bone components and polycaprolactone(PCL)using 3D printing is put forward.α1,3-galactosyltransferase deficient pigs were used as the donor source of a *** porcine bone(DCB)with attenuated immunogenicity was used as the natural component of the scaffold with the aim to promote bone *** 3D printed DCB-PCL scaffolds combined essential advantages such as uniformity of the interconnected macropores and high porosity and enhanced compressive *** biological properties of the DCB-PCL scaffolds were evaluated by studying cell adhesion,viability,alkaline phosphatase activity and osteogenic gene expression of human bone marrow-derived mesenchymal stem *** in vitro results demonstrated that the DCB-PCL scaffolds exhibit an enhanced performance in promoting bone differentiation,which is correlated to the DCB ***,critical-sized cranial rat defects were used to assess the effect of DCB-PCL scaffolds on bone regeneration in *** results confirm that in comparison with PCL scaffolds,the DCB-PCL scaffolds can significantly improve new bone formation in cranial ***,the proposed 3D printed DCB-PCL scaffolds emerge as a promising regeneration alternative in the clinical treatment of large bone defects.

关键词： Polycaprolactone 3D printing Decellularized porcine bone Cranial bone regeneration Attenuated immunogenicity

Transparent flexible thin-film p-n junction thermoelectric module

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npj Flexible Electronics 2020年第1期4卷 169-177页

作者： Xizu Wang Ady Suwardi Siew lay Lim Fengxia Wei Jianwei Xu Institute of Materials Research and Engineering(IMRE) Agency for ScienceTechnology and Research(A*STAR)#08-03Fusionopolis WayInnovisSingapore138634Singapore Department of Chemistry National University of Singapore3 Science Drive 3Singapore117543Singapore

Transparent and flexible thermoelectrics has been highly sought after for future wearable ***,the main stumbling block to prevent its widespread adoption is the lack of p-type transparent thermoelectrics and the stringent criteria of electrical and thermal properties matching appropriately between p-legs and *** work demonstrates the fabrication of p-type PEDOT:PSS films whose optical properties,electrical conductivity,thermal conductivity,and Seebeck coefficient were engineered to perfectly match the n-type indium tin oxide(ITO)*** dense p-type PEDOT:PSS and n-type ITO thin films show a thermoelectric figure of merit of zT=0.30 and 0.29 at 450 K,and a thermal conductivity of 0.22 and 0.32 W m^(−1) K^(−1),respectively.A flexible thermoelectric generator(TEG)module with a high transmittance of>81%in the visible wavelength range of 400-800 nm is fabricated using 10 pairs of p-type PEDOT:PSS and n-type ITO thin film *** ultra-high power density of 22.2 W m^(−2) at a temperature gradient of 80 K was observed,which is the highest power density reported for organic/hybrid-based flexible TEGs so *** transparent flexible thin-film p-n junction thermoelectric module with exceptionally high power generation may take a tremendous step forward towards multi-functional wearable devices.

关键词： film transparent exceptional