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Prevalence and Antibiotic Resistance of Urinary Tract Pathogens, with Molecular Identification of Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp., Using Multiplex Real-Time PCR

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American Journal of Molecular Biology 2024年第4期14卷 245-260页

作者： Hawa Tarnagda Djénéba Ouermi Tani Sagna Wendyam Marie Christelle Nadembega Abdoul Karim Ouattara Lassina Traoré Rogomenoma Alice Ouedraogo Prosper Bado Bapio Valérie Elvira Jean Télesphore Bazie nicole bouda/zongo Luc zongo Albert Théophane Yonli Théodora Mahoukèdè Zohoncon Florencia Wendkuuni Djigma Jacques Simpore Centre de Recherche Biomolculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso University Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso Laboratoire de Biologie Molculaire et de Gntique (LABIOGENE) Universit Joseph KI-ZERBO Ouagadougou Burkina Faso Institute for Research in Applied Sciences (IRSS/CNRST) Ouaga Burkina Faso Universit Norbert zongo/Centre Universitaire de Manga Avenue Maurice Yameogo Koudougou Burkina Faso Universit Nazi BONI Bobo-Dioulasso Burkina Faso Hpital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso

Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less tha

关键词： Urinary Tract Infections Klebsiella pneumoniae Klebsiella oxytoca Acinetobacter spp. Urine Culture Real-Time PCR

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Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (<i>Vigna subterranea</i>(L.) Verdc.) in Burkina Faso

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Advances in Bioscience and Biotechnology 2019年第9期10卷 270-281页

作者： Aboubié Elisabeth zongo Bouma James Néya Essowé Palanga Drissa Sérémé Moustapha Koala Régis Dimitri Sokpé Longué Jean Zabré Zakaria bouda Nicolas Barro Oumar Traoré Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA Ouagadougou Burkina Faso Université Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire National de Biosécurité Ouagadougou Burkina Faso

Potyviruses are major constraints to grain legume production by causing significant yield losses. Potyviruses infecting Bambara groundnut (Vigna subterranea) were investigated in Burkina Faso. Leaf samples collected from three agroclimatic zones were subjected to RT-PCR and sequence analyses. Of a total of 135 samples, 36 (26.67%) were detected positive in RT-PCR tests using potyvirus universal primers. Analysis of full coat protein (cp) sequences from 24 isolates revealed the occurrence of three groups of Bambara groundnut-infecting potyviruses. Virus isolates in group 1 shared 94.5% - 100% nucleotide (nt) identity with CABMV whereas those in group 2 and group 3 were distantly related Bean common necrosis virus (BCMNV) and Passiflora virus Ugandan which were their respective closest potyviruses. Group 2 shared 77.1% nt and 78.8% - 79.9% aa identity with BCMNV and group 3 shared 77.3% - 78.3% nt and 80.7% - 81.5% aa identity with Passiflora virus Ugandan. All three groups were confirmed by phylogenetic analyses. Taking into account potyvirus demarcation criteria, group 1 isolates belonged to CABMV species. Group 2 and group 3 were assigned to a potentially new potyviruses species and designated Bambara groundnut potyvirus 1 (BGPV1) and Bambara groundnut potyvirus 2 (BGPV2).

关键词： Bambara Groundnut Potyvirus Characterization Coat Protein Sequence Diversity

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Frequency and Factors Associated with Depression during Chronic Lumbosciatica in Sub-Saharan African Black Patients

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Open Journal of Rheumatology and Autoimmune Diseases 2020年第4期10卷 157-163页

作者： Charles Sougué Joëlle W. S. Tiendrébeogo Fulgence Kaboré Enselme Y. zongo nicole D. B. Ahongbonon Camille Sompougdou Yirozounlomian Tiaho Dieu-Donné Ouédraogo Department of Rheumatology University Hospital Center of Bogodogo Ouagadougou Burkina Faso Department of Internal Medicine Sour&#244 Sanou University Hospital Center Bobo Dioulasso Burkina Faso Department of Medicine Regional University Hospital Center of Ouahigouya Ouahigouya Burkina Faso Department of Physical Medicine and Rehabilitation University Hospital Center of Bogodogo Ouagadougou Burkina Faso

Objective: This study was focused on depression rate and associated factors in chronic lumbosciatica in sub-Saharan African black patients. Patients and Methods: We conducted a prospective descriptive and analytical study in chronic lumbosciatica cases seen in rheumatology/internal medicine, neurology and neurosurgery departement at the Yalgado ouédraogo University Hospital Center from April 31, 2016 to July 31, 2016. The diagnosis of depression was based on Hamilton scale. Its frequency was evaluated and the associated factors were analyzed. Results: One hundred and forty-nine patients were included in the study. Their average age was 49.5 years and the sex ratio was 0.42 (44 men/105 women). Thirty-eight patients lived alone (25.5%). Pain intensity was severe in 46 patients (30.9%) and moderate in 77 patients (51.7%). Neuropathic pain was present in 108 patients (72.5%). One hundred and ten patients had depression (73.8%). Depression was severe in 38 patients (25.5%), moderate in 35 patients (23.5%), and mild in 37 patients (24.8%). Factors associated with depression were living alone (Odds-Ratio 3.5), high (Odds-Ratio 11.1) or moderate (Odds-Ratio 5.9) intensity of pain, and the presence of neuropathic pain (Odds-Ratio 2.5). Conclusion: Depression was frequent during chronic lumbosciatica in sub-Saharan African black patients. Its associated factors were living alone, presence of moderate or severe pain, and neuropathic pain.

关键词： Depression Lumbosciatica Africa Burkina Faso

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