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Silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

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Neural Regeneration Research 2025年第6期20卷 1789-1800页

作者： Carina Hromada Patrick Heimel Markus Kerbl LászlóGál Sylvia Nürnberger barbara Schaedl James Ferguson nicole Swiadek Xavier Monforte Johannes C.Heinzel Antal Nógrádi Andreas H.Teuschl-Woller David Hercher Department Life Science Engineering University of Applied Sciences Technikum WienViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria Ludwig boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University Clinic of Dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medical University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of Hand- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tNGCs). However, the use of tNGCs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGC would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tNGCs and compared the various features of these improved silk^(+) tNGCs with the tubes without holes(silk^(–) tNGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tNGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tNGCs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tNGCs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tNGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimizatio

关键词： axon regeneration blood vessel functional recovery macroporous nerve lesion peripheral nerve repair sciatic nerve silk-based nerve guidance conduit vascularization

Plant peroxisomal solute transporter proteins

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Journal of Integrative Plant biology 2019年第7期61卷 817-835页

作者： Lennart Charton Anastasija Plett nicole Linka Institute for Plant biochemistry and Cluster of Excellence on Plant Sciences (CEPLAS) Heinrich Heine University

Plant peroxisomes are unique subcellular organelles which play an indispensable role in several key metabolic pathways, including fatty acid b-oxidation,photorespiration, and degradation of reactive oxygen species. The compartmentalization of metabolic pathways into peroxisomes is a strategy for organizing the metabolic network and improving pathway efficiency. An important prerequisite, however, is the exchange of metabolites between peroxisomes and other cell compartments. Since the first studies in the 1970s scientists contributed to understanding how solutes enter or leave this *** review gives an overview about our current knowledge of the solute permeability of peroxisomal membranes described in plants, yeast, mammals and other eukaryotes. In general, peroxisomes contain in their bilayer membrane specific transporters for hydrophobic fatty acids(AbC transporter) and large cofactor molecules(carrier for ATP, NAD and CoA). Smaller solutes with molecular masses below 300–400 Da, like the organic acids malate, oxaloacetate, and 2-oxoglutarate, are shuttled via non-selective channels across the peroxisomal *** comparison to yeast, human, mammals and other eukaryotes, the function of these known peroxisomal transporters and channels in plants are discussed in this review.

关键词： Plant peroxisomal solute transporter proteins

Slowed driving-reaction time following concussion-symptom resolution

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Journal of Sport and Health Science 2021年第2期10卷 145-153页

作者： Landon b.Lempke Robert C.Lynall nicole L.Hoffman Hannes Devos Julianne D.Schmidt UGA Concussion Research Laboratory Department of KinesiologyUniversity of GeorgiaAthensGA 30602USA School of Kinesiology and Recreation Illinois State UniversityNormalIL 61790USA Laboratory for Advanced Rehabilitation Research in Simulation Department of Physical Therapy and Rehabilitation ScienceUniversity of Kansas Medical CenterKansas CityKS 66160USA

background:Concussed patients have impaired reaction time(RT)and cognition following injury that may linger and impair driving *** research has used direct methods to assess driving-RT *** study compared driving RT during simulated scenarios between concussed and control individuals and examined driving-RT’s relationship with traditional computerized neurocognitive testing(CNT)***:We employed a cross-sectional study among 14 concussed(15.9±9.8 days post-concussion,mean±SD)individuals and 14 healthy controls matched for age,sex,and driving *** completed a driving simulator and CNT(CNS Vital Signs)assessment within 48 h of symptom resolution.A driving-RT composite(ms)was derived from 3 simulated driving scenarios:stoplight(green to yellow),evasion(avoiding approaching vehicle),and pedestrian(person running in front of vehicle).The CNT domains included verbal and visual memory;CNT-RT(simple-,complex-,Stroop-RT individually);simple and complex attention;motor,psychomotor,and processing speed;executive function;and cognitive *** t tests and Hedge d effect sizes assessed driving-RT differences between groups,Pearson correlations(r)examined driving RT and CNT domain relationships among cohorts separately,and p values were controlled for false discovery rate via benjamini-Hochberg procedures(a=0.05).Results:Concussed participants demonstrated slower driving-RT composite scores than controls(mean difference=292.86 ms;95%confidence interval(95%CI):70.18515.54;p=0.023;d=0.992).Evasion-RT(p=0.054;d=0.806),pedestrian-RT(p=0.258;d=0.312),and stoplight-RT(p=0.292;d=0.585)outcomes were not statistically significant after false-discovery rate corrections but demonstrated medium to large effect sizes for concussed *** concussed individuals,driving-RT outcomes did not significantly correlate with CNT domains(r-range:0.51 to 0.55;p>0.05).No correlations existed between driving-RT outcomes a

关键词： Mild traumatic brain injury Motor vehicle Neurocognitive function Response time Return to driving

Polyphenol composition and antioxidant potential of mint leaves

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Food Production, Processing and Nutrition 2019年第1期1卷 128-141页

作者： nicole brown Jenny AJohn Fereidoon Shahidi Department of biochemistry Memorial University of NewfoundlandSt.John’sNL A1B 3X9Canada Present Address:Department of Food Science and Technology Kerala University of Fisheries and Ocean StudiesKochiKerala 682506India

Soluble and insoluble/bound phenolic compounds and an aqueous infusion of two mint species(Medina and Hasawi),were tested for their total phenolic content,2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonate(AbTS)and 1,1-diphenyl-2picrylhydrazyl(DPPH)radical scavenging activity,iron(Ⅲ)reduction,iron(Ⅱ)chelating and oxygen radical absorbance capacity(ORAC).Furthermore,the potency of the mint extracts in the inhibition of radical-induced DNA scission,human low-density lipoprotein(LDL)cholesterol oxidation,formation of thiobarbituric acid reactive substances(TbARS)in a cooked ground meat system and LPS(lipopolysaccharide)-stimulated cyclooxygenase-2(COX-2)expression in J774A.1 mouse macrophage cells were *** showed that the soluble phenolics had a higher phenolic content and antioxidant activity than the insoluble-bound extracts and aqueous infusions in most of the *** varieties exhibited notable antioxidant activities and inhibition of LDL cholesterol oxidation,DNA scission and COX-2 gene expression at transcriptional ***,Medina mint was a more potent antioxidant than the Hasawi *** performance liquid chromatography with online tandem electrospray ionization mass spectrometry(HPLC/ESI-MS/MS)analysis of the extracts revealed that rosmarinic acid was the major phenolic compound present in both mint samples.

关键词： Mint Phenolic compounds Antioxidant activity Rosmarinic acid HPLC ESI-MS MS

Comparison of Cardiovascular Parameters and Internal Training Load of Different 1-h Training Sessions in Non-elite CrossFit^(®)Athletes

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Journal of Science in Sport and Exercise 2023年第2期5卷 130-141页

作者： nicole Meier Danien Sietmann Annette Schmidt Institut Für Sportwissenschaft Fakultät Für HumanwissenschaftenUniversität Der Bundeswehr MünchenWerner‑Heisenberg‑Weg 3985577 NeubibergGermany

Purpose The fact that CrossFit^(®)is the best-known and rapidly growing concept for high-intensity interval training(HIIT)and high-intensity functional training(HIFT)results in a continuous increase of athletes performing CrossFit^(®).In the more than 15,000 CrossFit^(®)Affiliates worldwide,the training concept is usually offered in 1-h training sessions containing the CrossFit^(®)-related workout of the day(WOD),as well as a general warm-up,movement demonstrations,and skill ***,we report how physiological parameters measured by heart rate(HR)values vary during four different 1-h CrossFit^(®)training sessions of non-elite athletes(n=27)in a local affiliated training center and what influencing factors may *** The duration of the 1-h training sessions were divided into a warm-up part(WU-part),a skill development part combined with strength exercises(A-part),followed by the WOD part(b-part).Results Analysis of HR values shows high training intensity(≥91%HR_(max))not throughout the duration of each training session,only during *** mean HR values in b-part differ significantly compared to the remaining training parts(Pbetween beginner and experienced CrossFit^(®)*** Our results may suggest that practicing CrossFit^(®)in 1-h training sessions combined anaerobic and aerobic exercise intensities,with the training concept allows beginners and experienced athletes to be trained with the same cardio-vascular responses and training intensities.

关键词： CrossFit^(®)performance Training load Exercise intensity Cardiovascular response High-intensity functional training

Unexpected ecotone dynamics of a sand dune vegetation complex following water table decline

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Journal of Plant Ecology 2016年第1期9卷 40-50页

作者： Csaba Tölgyesi Márta Zalatnai LászlóErdős Zoltán bátori nicole Rosemary Hupp LászlóKörmöczi Department of Ecology University of SzegedSzeged H-6726Hungary Wildlife biology Program College of Forestry and ConservationUniversity of Montana32 Campus DriveMissoulaMontanaMT 59812USA

Aims Central Hungarian inland dune ranges harbor heterogeneous grassland vegetation with an extensive network of ecotones,arranged perpendicular to topography-driven hydrologic *** area suffers from severe aridification due to climate change and local anthropogenic factors,which have led to a dramatic decline of the water *** a result,groundwater is no longer reachable for lowlying plant communities;thus,we expect they are bound to undergo profound *** study investigates how the plant communities respond to this changing environment over time by monitoring ecotones,since they are frequently the hotspots of ecosystem *** monitored five ecotones along permanent belt transects for 15 years to characterize their dynamic response,and to identify the internal structural changes of the plant communities the ecotones *** Ecotones were delineated with the split moving window *** dynamics of two ecotone parameters,location and contrast,were analyzed with linear regression models incorporating two independent variables:study year as a measure of time since the loss of groundwater,and precipitation as a possible driver of interannual *** internal changes of the patches separated by the ecotones were analyzed using plant functional *** Findings Precipitation had no detectable effect on the ecotone descriptors,but study year influenced ecotones in an unusual *** position of the ecotones appeared to be very stable in time;their dynamics are stationary,not directional as we *** contrasts had clear tendencies;two ecotones disappeared,one new one was formed and two ecotones showed no *** internal changes of the patches over time were dramatic,showing a shift toward more xeric and more open plant assemblages in most stretches of the ***,the dynamic response of the vegetation was not patch expansion ***,but fusion ***,which profoundly restructured the vegetati

关键词： groundwater decline plant functional group sand dune vegetation split moving window stationary ecotone

MicroRNA-let-7a promotes E2F-mediated cell proliferation and NFKb activation in vitro

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Cellular & Molecular Immunology 2014年第1期11卷 79-93页

作者： Cristen b Chafin nicole L Regna David L Caudell Christopher M Reilly Department of biomedical Sciences & Pathobiology Virginia-Maryland Regional College of Veterinary Medicine Virginia Polytechnic Institute and StateUniversity Blacksburg VA USA Department of Pathology/Comparative Medicine Wake Forest University Primate Center Wake Forest School ofMedicine Winston-Salem NC USA Edward Via College of Osteopathic Medicine Blacksburg VA USA

Epigenetic factors, including altered microRNA （miRNA） expression, may contribute to aberrant immune cell function in systemic lupus erythematosus （SLE）. MiRNA-let-7a （let-7a） has been shown to directly alter cell cycle progression and proinflammatory cytokine production. Due to the crucial role of let-7a in cell division and inflammation, we investigated let-7a-mediated proliferation and NFKb translocation in J774A. 1 macrophages and MES 13 mesangial cells in vitro. In immune-stimulated cells transfected with let-7a, cell proliferation was significantly increased over time. There was a significant increase in the number of immune-stimulated cells in S and G2 phases. Immune-stimulated cells overexpressing let-7a had increased nuclear translocation of NFKb. bioinformatical analysis revealed that the E2F family, critical regulators of the G1-S transition, has potential binding sites for let-7a in their mRNA transcripts. Let-7a overexpression significantly increased the expression of the cell cycle activator E2F2 and increased retinoblastoma protein （Rb） phosphorylation in immune-stimulated cells. The cell cycle inhibitor E2F5 was significantly decreased in let-7a-transfected cells that were immune-stimulated. bioinformatical analysis revealed E2F2 and NFKb are transcription factors predicted to regulate the let-7a promoter. We analyzed transcriptional regulation of let-7a by real-time RT-PCR using chromatin immunoprecipitation with E2F2 and NFKb antibodies. There was an increase in E2F2 and NFKb binding in DNA enriched for the let-7a promoter in immune-stimulated cells. Silencing E2F2 or NFKb significantly decreased let-7a expression and IL-6 production in immune-stimulated cells. Taken together, our results suggest that overexpression of let-7a may contribute to hyperplasia and the proinflammatory response in SLE.

关键词： cell proliferation E2F microRNA-let-7a NF~:b systemic lupus erythematosus

Endoscopic ultrasound assessment and tissue acquisition of mediastinal and abdominal lymph nodes

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World Journal of Gastrointestinal Oncology 2021年第10期13卷 1475-1491页

作者： Giacomo Tamanini Anna Cominardi nicole brighi Pietro Fusaroli Andrea Lisotti Department of Gastroenterology Unit Hospital of ImolaUniversity of BolognaImola 40026BOItaly Department of Medical Oncology IRCCS Istituto Romagnolo per lo Studio dei Tumori(IRST)"Dino Amadori"Meldola 47014FCItaly

The differential diagnosis between benign and malignant lymph nodes(LNs)has a fundamental role in the characterization and staging of malignant conditions,as well as in subsequent patients’*** imaging modalities(*** tomography and magnetic resonance imaging)rely mainly on size;endoscopic ultrasound(EUS)criteria based on b-mode evaluation and Doppler features fail to adequately characterize with high specificity LNs *** introduction of EUS-elastography and contrast-enhanced harmonic EUS are useful techniques to increase the diagnostic yield in identifying metastatic LNs,to identify which suspicious LN should require pathological characterization and,finally,to target tissue ***-guided tissue acquisition(EUS-TA)is increasingly being used for diagnosing lymphadenopathy whenever the characterization modifies patients’subsequent management and when no superficial LN is *** target therapy are currently available(*** cancer,breast cancer),EUS-TA of malignant LNs could be required to identify tumor *** this field,both fine needle aspiration and biopsy needles are able to guarantee accurate results with almost perfect specificity and sub-optimal *** finally propose a diagnostic algorithm based on most recent,high-level evidence for the diagnostic approach to suspected LNs assessment.

关键词： Endoscopic ultrasound-guided tissue acquisition Endoscopic ultrasound-fine needle aspiration Endoscopic ultrasound-fine needle biopsy biopsy Aspiration Cancer

Neuromodulation for tinnitus treatment:an overview of invasive and non-invasive techniques

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Journal of Zhejiang University-Science b(biomedicine & biotechnology) 2019年第2期20卷 116-130页

作者： nicole PETER Tobias KLEINJUNG Department of Otorhinolaryngology Head and Neck SurgeryUniversity Hospital Zürich

Tinnitus is defined as a perception of sound without any external sound *** tinnitus is a frequent condition that can affect the quality of *** far,no causal cure for tinnitus has been documented,and most pharmacologic and psychosomatic treatment modalities aim to diminish tinnitus’impact on the quality of ***,a novel therapeutic modality,which aims at alternating nerve activity through a targeted delivery of a stimulus,has emerged as a potential option in tinnitus *** review provides a brief overview of the current neuromodulation techniques as tinnitus treatment *** main intention is to provide updated knowledge especially for medical professionals counselling tinnitus patients in this emerging field of ***-invasive methods such as repetitive transcranial magnetic stimulation,transcranial electrical stimulation,neurofeedback,and transcutaneous vagus nerve stimulation were included,as well as invasive methods such as implanted vagus nerve stimulation and invasive brain *** of these neuromodulation techniques revealed promising results;nevertheless,further research is needed,especially regarding the pathophysiological principle as to how these neuromodulation techniques work and what neuronal change they *** studies suggest that individually different brain states and networks are involved in the generation and perception of ***,in the future,individually tailored neuromodulation strategies could be a promising approach in tinnitus treatment for achieving a more substantial and longer lasting improvement of complaints.

关键词： Tinnitus Neuromodulation Invasive technique Non-invasive technique