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Single nucleotide polymorphisms of OCTN1, OCTN2, and DLG5 genes in Greek patients with Crohn's disease

World Journal of Gastroenterology 2005年第47期11卷 7525-7530页

作者： Maria Gazouli Gerassimos Mantzaris Athanassios J Archimandritis George Nasioulas nicholas p anagnou Department of Biology School of MedicineUniversity of AthensAthens 11725Greece Department of Gastroenterology Evagelismos HospitalAthens 11521Greece Department of Internal Medicine Hippokration General HospitalSchool of MedicineUniversity of AthensAthens 11527Greece Molecular Biology Department Research Center HYGEIA "Antonis papayiannis" AthensGreece

AIM: To validate novel single nucleotide polymorphisms (SNps) in Greek patients with Crohn's disease (CD).METHODS: A total of 120 patients with CD, 85 patients with UC, and 100 unrelated healthy controls were genotyped. Genotyping was performed by allele-specific pCR or by pCR-RFLp ***: Our results showed that the 1672T and -207C alleles were obviously over-represented in CD patients only (p＜0.01 and p＜0.05, respectively) compared to the control population. The G113A polymorphism was completely absent in our studied population. The odds ratio for the carriage of the TC haplotype was 2.21 for CD patients as compared with controls. Additionally, the frequency of the TC haplotype was increased in patients with ileocolitis or colitis, and was mainly associated with the fibrostenotic phenotype of the disease. Furthermore, when the TC haplotype was compared jointly with the carriage of at least one mutation of the NOD2/CARD15 gene, there was an increased risk for CD, but not for UC, compared to controls. Regarding the location of the disease, the concomitant presence of the TC haplotype and NOD2/CARD15 mutations was mainly associated with ileocolitis or ileitis. CONCLUSION: Collectively, our results suggest that the 1672T variant of the OCTN1 gene and the -207C variant of the OCTN2 gene represent risk factors for CD in the Greek population.

关键词： Crohn's disease SNps OCTN1 OCTN2 DLG5

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NOD2/CARD15 , ATG16L1 and IL23R gene polymorphisms and childhood-onset of Crohn’s disease

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World Journal of Gastroenterology 2010年第14期16卷 1753-1758页

作者： Maria Gazouli Ioanna pachoula Ioanna panayotou Gerassimos Mantzaris George Chrousos nicholas p anagnou Eleftheria Roma-Giannikou Department of Biology School of Medicine University of Athens Athens 11527 Greece Laboratory of Cell and Gene Therapy Center of Basic Research Ⅱ Foundation for Biomedical Research of the Academy of Athens (IIBEAA) Athens 11527 Greece First Department of pediatrics "Aghia Sophia" Children's Hospital School of Medicine University of Athens Athens 11527 Greece Department of Gastroenterology Evagelismos Hospital Athens 11521 Greece

AIM: To assess whether the polymorphisms of NOD2/ CARD15 , autophagy-related 16-like 1 (ATG16L1 ), and interleukin-23 receptor (IL23R ) genes play a more critical role in the susceptibility of childhood-onset than in adult-onset Crohn’s disease (CD). METHODS: polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 ; rs2241880 A/G of ATG16L1 , and rs11209026 (R381Q) of IL23R gene were assessed in 110 childhood-onset CD, 364 adult-onset CD, and 539 healthy individuals. Analysis of polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 genotyping was performed by allele specific polymerase chain reaction (pCR) or by pCR-restriction fragment length polymor-phism analysis. The polymorphisms rs2241880 A/G of the ATG16L1 , and rs11209026 (R381Q) of the IL23R gene in the children’s cohort were genotyped by pCR and melting curve analysis whereas adult group genotyping was performed using the Affymetrix Genome-Wide Human SNp Array 5.0 (500K). RESULTS: The 3020insC allele in NOD2/CARD15 was significantly higher in childhood than in adult-onset CD (p = 0.0067). Association with at least 1 NOD2/CARD15 variant was specific for ileal disease (with or without co- lonic involvement). Even if the frequency of G allele of the rs2241880 ATG16L1 polymorphism was increased in both paediatric and adult CD patients compared to con- trols (p = 0.017 and p = 0.001, respectively), no difference was observed between the childhood and the adult cohort. The rare Q allele of IL23R rs11209026 polymorphism was underrepresented in both paediatric and adult CD cases (p = 0.0018 and p = 0.04, respectively) and no difference was observed between the childhood and the adult cohort. The presence of the rs2241880 ATG16L1 and rs11209026 IL23R polymorphisms did not influence disease phenotype. CONCLUSION: polymorphism 3020insC in NOD2/ CARD15 occurs statistically significantly more often in patients with childhood-onset CD than in patients with adult-onset CD. The ATG16L1 and IL23R variants are associated

关键词： Genetics Childhood-onset Inflammatory bowel disease Crohn’s disease Genetic susceptibility NOD2/CARD15 ATG16L1 IL23R polymorphisms

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Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection

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World Journal of Gastroenterology 2012年第32期18卷 4419-4426页

作者： Maria Gazouli Anna Lyberopoulou pericles pericleous Spyros Rizos Gerassimos Aravantinos Nikolaos Nikiteas nicholas p anagnou Efstathios p Efstathopoulos Department of Basic Medical Sciences Laboratory of BiologySchool of MedicineUniversity of Athens11527 AthensGreece First Department of Surgery "Tzaneion" General Hospital18536 PiraeusGreece Third Department of Internal Medicine Agii Anargyri Hospital14564 AthensGreece Second Department of propedeutic Surgery University of Athens School of MedicineLaiko General Hospital11527 AthensGreece Department of Radiology Attikon University Hospital12462 AthensGreece

AIM:To detect of colorectal cancer(CRC) circulating tumour cells(CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots(QDs) in these ***:The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs,using specific antibody biomarkers:epithelial cell adhesion molecule antibody,and monoclonal cytokeratin 19 *** detection signal was acquired from the fluorescence signal of *** the evaluation of the performance,the method under study was used to isolate the human colon adenocarcinoma cell line(DLD-1) and CTCs from CRC patients' peripheral ***:The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a *** cell sorting analysis and Real Time RT-pCR,they both have also been used to evaluate the performance of the described *** conclusion,we developed a simple,sensitive,efficient and of lower cost(than the existing ones) method for the detection of CRC CTCs in human *** have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence ***:The method described here can be easily adjusted for any other protein target of either the CTC or the host.

关键词： Circulating tumor cells Cancer Quantum dots Nanoprobes Micrometastasis

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Neoadjuvant treatment of esophageal cancer

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World Journal of Gastroenterology 2010年第30期16卷 3793-3803页

作者： nicholas p Campbell Victoria M Villaflor Section of Hematology/Oncology Department of MedicineThe University of Chicago Pritzker School of MedicineChicagoIL 60637United States

The management of esophageal cancer has been evolving over the past 30 years. In the United States, multimodality treatment combining chemotherapy and radiotherapy (RT) prior to surgical resection has come to be accepted by many as the standard of care, although debate about its overall effect on survival still exists, and rightfully so. Despite recent improvements in detection and treatment, the overall survival of patients with esophageal cancer remains lower than most solid tumors, which highlights why further advances are so desperately needed. The aim of this article is to provide a complete review of the history of esophageal cancer treatment with the addition of chemotherapy, RT, and more recently, targeted agents to the surgical management of resectable disease.

关键词： Esophageal cancer Multimodality therapy Neoadjuvant therapy Chemotherapy Radiotherapy Targeted agents Disease management

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Shaping Taste: The Molecular Discovery of Rice Genes Improving Grain Size, Shape and Quality

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Journal of Genetics and Genomics 2015年第11期42卷 597-599页

作者： nicholas p.Harberd Department of plant Sciences University of Oxford

Modern-day human life is absolutely dependent upon the food that we derive from our crop plants. We eat grains, fruits, roots, tubers and other structures, all of which are constructed via coordinated organ growth. Whilst plant organ identity is first established in apical meristems （vegetative and floral shoot meristems and root meristems）, and in other meristematic regions, the final size and shape of organs are defined by subsequent coordination of organ expansion in longitudinal and transverse axes.

关键词： QTLs Shaping Taste The Molecular Discovery of Rice Genes Improving Grain Size Shape and Quality gene

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Anthropogenic Influence on Decadal Changes in Concurrent Hot and Dry Events over China around the Mid-1990s

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Advances in Atmospheric Sciences 2024年第2期41卷 233-246页

作者： Qin SU Buwen DONG Fangxing TIAN nicholas p.KLINGAMAN Yunnan Key Laboratory of Meteorological Disasters and Climate Resources in the Great Mekong Subregion Yunnan UniversityKunming 650500China Department of Atmospheric Sciences Yunnan UniversityKunming 650500China National Centre for Atmospheric Science Department of MeteorologyUniversity of ReadingReadingRG66BBUK Geofysisk Institut University of BergenBergen 5007Norway Citadel Investment Group Moor House120 London WallLondonRG66BBUK

The frequency and duration of observed concurrent hot and dry events(HDEs) over China during the growing season(April–September) exhibit significant decadal changes across the mid-1990s. These changes are characterized by increases in HDE frequency and duration over most of China, with relatively large increases over southeastern China(SEC), northern China(NC), and northeastern China(NEC). The frequency of HDEs averaged over China in the present day(pD,1994–2011) is double that in the early period(Ep, 1964–81);the duration of HDEs increases by 60%. Climate experiments with the Met Office Unified Model(MetUM-GOML2) are used to estimate the contributions of anthropogenic forcing to HDE decadal changes over China. Anthropogenic forcing changes can explain 60%–70% of the observed decadal changes,suggesting an important anthropogenic influence on HDE changes over China across the mid-1990s. Single-forcing experiments indicate that the increase in greenhouse gas(GHG) concentrations dominates the simulated decadal changes,increasing the frequency and duration of HDEs throughout China. The change in anthropogenic aerosol(AA) emissions significantly decreases the frequency and duration of HDEs over SEC and NC, but the magnitude of the decrease is much smaller than the increase induced by GHGs. The changes in HDEs in response to anthropogenic forcing are mainly due to the response of climatological mean surface air temperatures. The contributions from changes in variability and changes in climatological mean soil moisture and evapotranspiration are relatively small. The physical processes associated with the response of HDEs to GHG and AA changes are also revealed.

关键词： concurrent hot and dry events decadal variation greenhouse gases aerosol emissions

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On Northern Hemisphere Wave patterns Associated with Winter Rainfall Events in China

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Advances in Atmospheric Sciences 2018年第8期35卷 125-138页

作者： Claudia Christine STEpHAN Yan Ho NG nicholas p. KLINGAMAN National Centre for Atmospheric Science - Climate Department of Meteorology University of Reading Reading RG6 6BB United Kingdom Department of Meteorology University of Reading Reading RG6 6BB United Kingdom

During extended winter （November-April）, 43% of the intraseasonal rainfall variability in China is explained by three spatial patterns of temporally coherent rainfall, These patterns were identified with empirical orthogonal teleconnection （EOT） analysis of observed 1982-2007 pentad rainfall anomalies and connected to midlatitude disturbances. However, ex- amination of individual strong EOT events shows that there is substantial inter-event variability in their dynamical evolution, which implies that precursor patterns found in regressions cannot serve as useful predictors. To understand the physical nature and origins of the extratropical precursors, the EOT technique is applied to six simulations of the Met Office Unified Model at horizontal resolutions of 200-40 km, with and without air-sea coupling. All simulations reproduce the observed precursor patterns in regressions, indicating robust underlying dynamical processes. Further investigation into the dynamics associated with observed patterns shows that Rossby wave dynamics can explain the large inter-event variability. The results suggest that the appaxently slowly evolving or quasi-stationaxy waves in regression analysis are a statistical amalgamation of more rapidly propagating waves with a variety of origins and properties.

关键词： rainfall in China spring flooding Rossby wave dynamics EOT analysis predictability teleconnections

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A polarizing situation： Taking an in-plane perspective for next-generation near-field studies

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Frontiers of physics 2016年第2期11卷 1-17页

作者： p. James Schuck Wel Bao nicholas J. Borys Molecular Foundry Lawrence Berkeley National Laboratory Berkeley CA 94720 USA Department of Materials Science and Engineering University of California Berkeley CA 94720-1760 USA

By enabling the probing of light-matter interactions at the functionally relevant length scales of most materials, near-field optical imaging and spectroscopy accesses information that is unobtainable with other methods. The advent of apertureless techniques, which exploit the ultralocalized and enhanced near-fields created by sharp metallic tips or plasmonic nanoparticles, has resulted in rapid adoption of near-field approaches for studying novel materials and phenomena, with spatial resolution approaching sub-molecular levels. However, these approaches are generally limited by the dominant out-of-plane polarization response of apertureless tips, restricting the exploration and discovery of many material properties. This has led to recent design and fabrication breakthroughs in near-field tips engineered specifically for enhancing in-plane interactions with near-field light components. This mini-review provides a perspective on recent progress and emerging directions aimed at utilizing and controlling in-plane optical polarization, highlighting key application spaces where in-plane near-field tip responses have enabled recent advancements in the understanding and development of new nanostructured materials and devices.

关键词： near-field optical microscopy nano-optics TERS plasmonics optical antenna 2D materials

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Green Revolution DELLAs: From translational reinitiation to future sustainable agriculture

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Molecular plant 2021年第4期14卷 547-549页

作者： Qian Liu Kun Wu nicholas p.Harberd Xiangdong Fu State Key Laboratory of plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Department of plant Sciences University of OxfordSouth Parks RoadOxford 0X13RBUK College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

The Green Revolution(GR)of the 1960s saw spectacular increases in cereal crop yields,enabled by widespread adoption of high-yielding semi-dwarf varieties and increased fertilizer ***-dwarfism is caused by mutant wheat Reduced height-1(Rht-1)and rice semi-dwarf1(sd1)alleles,respectively,which confer increased harvest index(grain to straw ratio)and reduced yield loss from“lodging”(flattening of crops by wind or rain).However,despite their obvious global food security benefits,the underlying mechanisms via which GR dwarfing alleles actually work remained *** began to change with the molecular discovery of Rht1(peng et al.,1999).

关键词： dwarf crops cereal

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Travellers:Design Collaboration and Digital Media

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Tsinghua Science and Technology 2008年第S1期13卷 234-240页

作者： J. Bahoric p. nicholas G. Ormston Arup pty Ltd Spatial Information Architecture Laboratory RMIT University Melbourne 3000 Australia City of Melbourne

This paper examines the use of 3D electronic medium as a tool for procuring detailed design information for the Sandridge Bridge Travellers project. The Sandridge Bridge Travellers project required the production of 10 7.5 m of tall × 5 ― 12 m of wide figures under very tight programme and budget constraints. This was achieved through a synthesis of art and engineering, whereby structure and sculpture become one and the same. Traditional procurement routes would have entailed the production and interpretation of drawings and design details at all discipline interfaces before fabrication; an approach that was unfeasible given the constraints. Instead, The Travellers project saw a single electronic file pass backwards and forwards from client to architect, engineer, and fabricator, with information from the initial artistic concept work phases through to analysis, design, member scheduling and fabrication all integrated and facilitated by a single electronic medium. Key to the delivery was the use of 3D modeling and scripting programs that enabled the efficient use and development of design information through all stages of the design process. This enabled total integration of all trades requiring a collaborative approach where each party maintained joint ownership of the 3D design information from inception to conception.

关键词： Sandridge Bridge Travellers sculpture 3-D modeling collaborative approach 3-D electronic medium AutoCAD R2004TM RhinoTM formats spline rationalization

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