检索结果-南通市图书馆

3′-UTR of the SARS-CoV-2 genome as a possible source of piRNAs

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第3期10卷 668-670页

作者： María Teresa Hernández-huerta Laura Pérez-Campos Mayoral Carlos Alberto Matias-Cervantes Carlos Romero Díaz Eli cruz Parada Eduardo Pérez-Campos Mayoral Rafael Baltiérrez-Hoyos Margarito Martínez cruz Gabriel Mayoral Andrade Eduardo Pérez-Campos CONACyT Faculty of Medicine and SurgeryAutonomous University“Benito Juárez”of Oaxaca(UABJO)Oaxaca 68020Mexico Research Center Faculty of Medicine UNAM-UABJOAutonomous University“Benito Juárez”of Oaxaca(UABJO)Oaxaca 8020Mexico National Technology of Mexico/IT Oaxaca Oaxaca de JuárezOaxaca 68030Mexico

SARS-CoV-2 is the causal agent of the COVID-19 pandemic. This is a single-stranded RNA beta-coronavirus that is comprised of a 5′ and 3′-untranslated region (UTR) in its non-coding RNA (ncRNA) region. The P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are ncRNA sequences that bind to transposons and interfere with the translation of new genes. Here, we search for short sequences in the non-coding regions, corresponding to 28 nucleotides (nt). These start from a uracil and have a conserved region of at least 10 nt of homology with the previously reported piRNAs. The aim of this work was to identify piRNA-like sequences in the 3′-UTR of SARS-CoV-2 from the reported Wuhan Patient's genome sequence (GenBank: MN908947.3).

关键词： piRNA sequences piRNAs

Pathways of Phosphorus Absorption and Early Signaling between the Mycorrhizal Fungi and Plants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2021年第5期90卷 1321-1338页

作者： Griselda Madrid-Delgado Marcos Orozco-Miranda Mario cruz-Osorio Ofelia Adriana Hernández-Rodríguez Raúl Rodríguez-Heredia Melchor Roa-huerta Graciela Dolores Avila-Quezada Facultad de Ciencias Agrotecnológicas Universidad Autónoma de Chihuahua(UACH)Chihuahua31000México Innovak Global Chihuahua31375México

This review highlights the key role that mycorrhizal fungi play in making phosphorus(Pi)more available to plants,including pathways of phosphorus absorption,phosphate transporters and plant-mycorrhizal fungus symbiosis,especially in conditions where the level of inorganic phosphorus(Pi)in the soil is *** fungi colonization involves a series of signaling where the plant root exudates strigolactones,while the mycorrhizal fungi release a mixture of chito-oligosaccharides and liposaccharides,that activate the symbiosis process through gene signaling pathways,and contact between the hyphae and the *** the symbiosis is established,the extraradical mycelium acts as an extension of the roots and increases the absorption of nutrients,particularly phosphorus by the phosphate *** then moves along the hyphae to the plant root/fungus *** transfer of Pi occurs in the apoplectic space;in the case of arbuscular mycorrhizal fungi,Pi is discharged from the arbuscular to the plant’s root symplasm,in the membrane that surrounds the *** is then absorbed through the plant periarbuscular membrane by plant phosphate ***,plants can acquire Pi from soil as a direct absorption *** a result of this review,several genes that codify for high-affinity Pi transporters were *** plants,the main family is Pht1 although it is possible to find others such as Pht2,Pht3,Pho1 and *** in plants,mycorrhizal fungi have genes belonging to the Pht1 *** arbuscular mycorrhizal fungi we found L1PT1,GiPT,MtPT1,MtPT2,MtPT4,HvPT8,ZmPht1,TaPTH1.2,GmosPT and ***1,HcPT2 and BePT have been characterized in ectomycorrhizal *** gene has a different way of expressing *** this review,we present diagrams of the symbiotic relationship between mycorrhizal fungi and the *** knowledge allows us to design solutions to regional problems such as food production in soils with low levels of Pi.

关键词： Interaction,mycorrhiza establishment,symbiosis phosphate uptake

Electrospinning Polylactic Acid Polymer Membranes as Biological Sieve for Yeast and Bacteria

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Materials Sciences and Applications 2022年第6期13卷 389-400页

作者： Ernesto Suaste-Gómez Grissel Rodríguez-Roldán Ilian Pérez-Solis Ana Laura Torres-huerta Carlos cruz-cruz José Tapia-Ramírez Department of Electrical Engineering Section of Bioelectronics Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV) Mexico City Mexico National Laboratory of Experimental Services (LaNSE) CINVESTAV Mexico City Mexico Department of Genetics and Molecular Biology CINVESTAV Mexico City Mexico

Vaginal infections are suffered by millions of women of reproductive age. Recent research has shown that timely diagnosis of these infections avoids complications in the patient’s health. Since the symptoms of infections caused by yeast and bacteria are similar, the patient is not always treated properly. Membranes with three different thicknesses (66 μm, 83 μm, 128 μm) were produced by electrospinning of polylactic polymer (PLA) and were used as a biological sieve to detect microorganisms present in vaginal infections, bacteria or yeasts. Electrospinning technique is a technique that allows the generation of membranes from nanofibers of material polymeric and therefore, obtaining porous structures. The size of the fibers was observed as well as the morphology and the size of the pores, using the scanning electron microscopy (SEM). The effectiveness of these membranes was proven by the cultivation of the bacteria that passed through the polymeric membrane and the yeasts retained by it. The membranes allowed the retention of yeasts of Candida albicans type with dimensions of 7 μm, which allowed the separation of Brevibacterium ravens purgense bacteria with dimensions of 1.4 μm, thus demonstrating their effectiveness as biological sieve.

关键词： Biological Sieve Electrospinning Polylactic Acid Vaginal Infections

Interaction of piRNA-like sequences from the 3′-UTR of SARS-CoV-2 with mRNA regions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第6期10卷 2282-2284页

作者： Laura Pérez-Campos Mayoral María Teresa Hernández-huerta Carlos Romero Díaz Carlos Alberto Matias-Cervantes Eduardo Pérez-Campos Mayoral Margarito Martínez cruz Juan de Dios Ruiz-Rosado Edgar Gustavo Ramos Martínez Marco Antonio Sánchez Medina Eduardo Pérez-Campos Research Center Faculty of Medicine UNAM-UABJOAutonomous University“Benito Juárez”of Oaxaca(UABJO)Oaxaca 68020Mexico CONACyT Faculty of Medicine and Surgery.Autonomous University“Benito Juárez”of Oaxaca(UABJO)Oaxaca 68020Mexico National Technology of Mexico(TecNM)/IT Oaxaca Oaxaca de JuárezOaxaca 68030Mexico The Research Institute at Nationwide Children's Hospital Columbus 43205USA Facultad de Química Universidad Nacional Autónoma de MéxicoCoyoacán 04510Mexico

Piwi-interacting RNAs(piRNAs of 26-32 nt in length)regulate gene expression,epigenetics,and transcriptional and post-transcriptional processes.1 In humans,piRNAs are expressed in germline and somatic tissues,and generally have a uracil at the 5'-end(position+1),an adenine at the+10 position,and are 2'-0 methylated at the 3'-end.2 Onice piRNAs form the RNA-induced silencing complex(RISC)with PIWl proteins,they serve as guides to find their complementary sequences of the target mRNA,thus initiating their *** Wuhan patient's genome is approximately 265 nucleotides(nts)in its 3'-UTR;piRNAs-like sequences have been reported in this region.3 Our objective was to search,through bioinformatics,for mRNA sequences that were homologous to the reverse complementary of the previously reported piRNA-like sequences from the 3'-UTR of SARS-Cov-2,3 and identify the possible interaction between them.

关键词： sequences complementary initiating

Cerebrolysin Effects on Cardiac Neuropathy in Diabetic Rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pharmacology & Pharmacy 2017年第7期8卷 215-230页

作者： Enedina Zurita Maribel huerta Luis De Jesús Fidel De La cruz Rocio Ortiz-Butron Gonzalo Flores Ma De Jesús Gómez-Villalobos Depto. de Fisiología Escuela Nacional de Ciencias Biológicas IPN Unidad Profesional Adolfo López Mateos (Zacatenco) Wilfrido Massieu S/N. Col. Nueva Industrial Vallejo Delegación Gustavo A. Madero. CP 07700. Cd. De México Mexico Instituto de Fisiología 14 Sur 6301 Col. San Manuel. CP 72570 Benemérita Universidad Autónoma de Puebla Puebla Mexico

Autonomic innervation of heart is abnormal in diabetes and produces altered cardiovascular parameters. Cerebrolysin is a neurotrophic factor that improves the dendritic tree and synapses in the central nerve system after brain damage. The aim of this study was to evaluate if cerebrolysin can improve the cardiac neuropathy generated in diabetic rats. Male Sprague-Dawley rats two months old were injected with streptozotocin (70 mg/Kg/, ip). Hyperglycemia and altered cardiac rate were confirmed after eight weeks of STZ injection, and cerebrolysin treatment was started in control and diabetic rats for two months (1 ml/kg/day, ip). Body weight, heart rate, heart rate variability, arterial blood pressure, and blood glucose levels were measured. Also heart weight and levels of nitrites, NGF and VEGF were measured in left ventricle homogenates. The results show that body weight was reduced and blood glucose levels were increased significantly in diabetic rats. Cerebrolysin treatment produced no significant changes in body weight either in blood glucose level in control and diabetic rats. Cerebrolysin treatment in diabetic rats shows an improvement in the altered basal cardiac rate (306 ± 6.5 lat/min) compared to diabetic saline group (272 ± 8.9 lat/min: P < 0.05), without changes in control rats. Levels of nitrites, VEGF, and NGF in the left ventricle increased in diabetic cerebrolysin treated rats. In conclusion, the results show that cerebrolysin improves some abnormalities observed in the diabetic cardiac neuropathy in rats and suggest that could be considered an additional treatment to prevent or reduce the cardiac autonomic alterations generated in diabetes.

关键词： Autonomic Innervation Heart Hyperglycemia Cerebrolysin Rats