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The RNA editome of Macaca mulatta and functional characterization of RNA editing in mitochondria

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Science Bulletin 2017年第12期62卷 820-830页

作者： Ling-Qun Ye Hui Zhao He-JiangZhou Xiao-Die Ren Lin-Lin Liu Newton O.OTECKO Zheng-boWang Min-Min Yang Lin Zeng Xin-Tian Hu Yong-Gang Yao Ya-Ping Zhang Dong-Dong Wu State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan UniversityKunming 650091China Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of Sciences Kunming 650223China Kunming College of Life Science University of Chinese Academy of Scienceskunming 650204China Kunming Primate Research Center of the Chinese Academy of Sciences Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China

RNA editing was first discovered in mitochondrial RNA molecular. However, whether adenosine-toinosine(A-to-I) RNA editing has functions in nuclear genes involved in mitochondria remains elusive.Here, we retrieved 707,246 A-to-I RNA editing sites in Macaca mulatta leveraging massive transcriptomes of 30 different tissues and genomes of nine tissues, together with the reported data, and found that A-to-I RNA editing occurred frequently in nuclear genes that have functions in mitochondria. The mitochondrial structure, the level of ATP production, and the expression of some key genes involved in mitochondrial function were dysregulated after knocking down the expression of ADAR1 and ADAR2, the key genes encoding the enzyme responsible for RNA editing. When investigating dynamic changes of RNA editing during brain development, an amino-acid-changing RNA editing site(I234/V) in MFN1, a mediator of mitochondrial fusion, was identified to be significantly correlated with age, and could influence the function of MFN1. When studying transcriptomes of brain disorder, we found that dysregulated RNA editing sites in autism were also enriched within genes having mitochondrial functions. These data indicated that RNA editing had a significant function in mitochondria via their influence on nuclear genes.

关键词：线粒体RNA RNA编辑酶猕猴特性编辑位点核基因体功能动态变化

origin of new genes after zygotic genome activation in vertebrate

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Journal of Molecular Cell Biology 2018年第2期10卷 139-146页

作者： Hai-Bo Xu Yong-Xin Li Yan Li newton o. otecko Ya-Ping Zhang Bingyu Mao Dong-Dong Wu State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming China College of Life Science Anhui University Hefei China Kunming College of Life Science University of Chinese Academy of Sciences Kunming China State Key Laboratory for Conservation and Utilization of Bio-resource Yunnan University Kunming China

New genes are drivers of evolutionary innovation and phenotypic evolution. Expression of new genes in early development raises the possibility that new genes could originate and be recruited for functions in embryonic development, but this remains undocumented. Here, based on temporal gene expression at different developmental stages inXenopus tropicalis, we found that young protein-coding genes were significantly enriched for expression in developmental stages occurring after the midblastula transition (MBT), and displayed a decreasing trend in abundance in the subsequent stages after MBT. To complement the finding, we demonstrate essential functional attributes of a young orphan gene, named asFog2, in morphological development. our data indicate that new genes could originate after MBT and be recruited for functions in embryonic development, and thus provide insights for better understanding of the origin, evolution, and function of new genes.

关键词：新基因脊椎动物起源染色体激活数据显示编码蛋白质 MBT

Suppressive effects of exercise-conditioned serum on cancer cells:A narrative review of the influence of exercise mode,volume,and intensity

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Journal of Sport and Health Science 2024年第4期13卷 484-498,F0003页

作者： Francesco Bettariga Dennis R.Taaffe Daniel A.Galvao Chris Bishop Jin-Soo Kim Robert U.newton Exercise Medicine Research Institute Edith Cowan UniversityJoondalupWA 6027Australia School of Medical and Health Sciences Edith Cowan UniversityJoondalupWA 6027Australia London Sport Institute School of Science and TechnologyMiddlesex UniversityLondonNW44BTUK School of Human Movement and Nutrition Sciences University of QueenslandSt.LuciaQLD 4072Australia

Cancer is a major cause of morbidity and mortality worldwide,and the incidence is increasing,highlighting the need for effective strategies to treat this disease.Exercise has emerged as fundamental therapeutic medicine in the management of cancer,associated with a lower risk of recur-rence and increased survival.Several avenues of research demonstrate reduction in growth,proliferation,and increased apoptosis of cancer cells,including breast,prostate,colorectal,and lung cancer,when cultured by serum collected after exercise in vitro(i.e.,the cultivation of cancer cell lines in an experimental setting,which simpliﬁes the biological system and provides mechanistic insight into cell responses).The underlying mechanisms of exercise-induced cancer suppressive effects may be attributed to the alteration in circulating factors,such as skeletal muscle-induced cytokines(i.e.,myokines)and hormones.However,exercise-induced tumor suppressive effects and detailed information about training interventions are not well investigated,constraining more precise application of exercise medicine within clinical oncology.To date,it remains unclear what role different training modes(i.e.,resistance and aerobic training)as well as volume and intensity have on exercise-condi-tioned serum and its effects on cancer cells.Nevertheless,the available evidence is that a single bout of aerobic training at moderate to vigorous intensity has cancer suppressive effects,while for chronic training interventions,exercise volume appears to be an inﬂuential candidate driving cancer inhibitory effects regardless of training mode.Insights for future research investigating training modes,volume and intensity are provided to further our understanding of the effects of exercise-conditioned serum on cancer cells.

关键词： Cancer cells High intensity interval training Moderate intensity continuous training Resistance training Myokines

A human-specific insertion promotes cell proliferation and migration by enhancing TBC1D8B expression

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Science China Life Sciences 2023年

作者： Hui Zhao Lin-Lin Liu Jian Sun Lian Jin Hai-Bing Xie Jian-Bo Li Hui Xu Dong-Dong Wu Xiao-Lin Zhuang Min-Sheng Peng Ya-Jun Guo Wei-Zhu Qian Newton O.OTECKO Wei-Jie Sun Liang-Hu Qu Jie He Zhao-Li Chen Rong Liu Ce-Shi Chen Ya-Ping Zhang State Key Laboratory of Genetic Resources and Evolution Yunnan Laboratory of Molecular Biology of Domestic Animals Kunming Institute of Zoology Chinese Academy of Sciences School of Forensic Medicine Kunming Medical University State Key Laboratory for Conservation and Utilization of Bio-resource School of Life Sciences School of Ecology and Environmental Science Yunnan University The Key Laboratory of Gene Engineering of the Ministry of Education State Key Laboratory for Biocontrol Sun Yat-sen University Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of Zoology Chinese Academy of Sciences Department of Thoracic Surgery Cancer Institute and Hospital Chinese Academy of Medical Sciences and Peking Union Medical College National Engineering Research Center for Antibody Medicine and Shanghai Key Laboratory of Cell Engineering and Antibody Academy of Biomedical Engineering Kunming Medical University The Third Affiliated Hospital Kunming Medical University

Human-specific insertions play important roles in human phenotypes and diseases. Here we reported a 446-bp insertion(Insert-446) in intron 11 of the TBC1D8B gene, located on chromosome X, and traced its origin to a portion of intron 6 of the EBF1 gene on chromosome 5.Interestingly, Insert-446 was present in the human Neanderthal and Denisovans genomes, and was fixed in humans after human-chimpanzee divergence. We have demonstrated that Insert-446 acts as an enhancer through binding transcript factors that promotes a higher expression of human TBC1D8B gene as compared with orthologs in macaques. In addition, over-expression TBC1D8B promoted cell proliferation and migration through “a dual finger” catalytic mechanism(Arg538 and Gln573) in the TBC domain in vitro and knockdown of TBC1D8B attenuated tumorigenesis in vivo. Knockout of Insert-446 prevented cell proliferation and migration in cancer and normal cells.our results reveal that the human-specific Insert-446 promotes cell proliferation and migration by upregulating the expression of TBC1D8B gene. These findings provide a significant insight into the effects of human-specific insertions on evolution.

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Time to consider the potential role of alternative resistance training methods in cancer management?

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Journal of Sport and Health Science 2023年第6期12卷 715-725页

作者： Francesco Bettariga Chris Bishop Dennis R.Taaffe Daniel A.Galvão Luca Maestroni Robert U.newton Exercise Medicine Research Institute Edith Cowan UniversityJoondalupWA 6027Australia School of Medical and Health Sciences Edith Cowan UniversityJoondalupWA 6027Australia London Sport Institute School of Science and TechnologyMiddlesex UniversityLondonNW44BTUK School of Human Movement and Nutrition Sciences University of QueenslandSt.LuciaQLD 4067Australia

Exercise has emerged as fundamental therapeutic medicine in the management of cancer.Exercise improves health-related outcomes,including quality of life,neuromuscular strength,physical function,and body composition,and it is associated with a lower risk of disease recurrence and increased survival.Moreover,exercise during or post cancer treatments is safe,can ameliorate treatment-related side effects,and may enhance the effectiveness of chemotherapy and radiation therapy.To date,traditional resistance training(RT)is the most used RT modality in exercise oncology.However,alternative training modes,such as eccentric,cluster set,and blood flow restriction are gaining increased attention.These training modalities have been extensively investigated in both athletic and clinical populations(e.g.,age-related frailty,cardiovascular disease,type 2 diabetes),showing considerable benefits in terms of neuromuscular strength,hypertrophy,body composition,and physical function.However,these training modes have only been partially or not at all investigated in cancer populations.Thus,this study outlines the benefits of these alternative RT methods in patients with cancer.Where evidence in cancer populations is sparse,we provide a robust rationale for the possible implementation of certain RT methods that have shown positive results in other clinical populations.Finally,we provide clinical insights for research that may guide future RT investigations in patients with cancer and suggest clear practical applications for targeted cancer populations and related benefits.

关键词： Blood flow restriction Cluster set Eccentric training Resistance trainingTagedAPTARAEnd

Population Genomic Insights into High Altitude Adaptation in Pamirs

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Population Genomic Insights into High Altitude Adaptation in...

Chronic exposure to high-density polyethylene microplastic through feeding alters the nutrient metabolism of juvenile yellow perch (Perca flavescens)

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Xierzhatijiang Sulaiman Min-Sheng Peng Wei-fang Xu Jin-Xiu Li Najmudinov Tojiddin Abdulloevich Manilova Elena Afanasevna Khudoidodov Behruz Ibrohimovich Xi Chen Wei-Kang Yang Xing-Yan Yang Quan-Kuan Shen Shi-Fang Wu Yun Gao Newton O.OTECKO Yong-Gang Yao Lu-Feng Cheng Ya-Ping Zhang Department of Pharmacology College of PharmacyXinjiang Medical University State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of Sciences Kunming College of Life Science University of Chinese Academy of Sciences KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Shenzhen Hospital of Guangzhou University of Chinese Medicine State Key Laboratory for Conservation and Utilization of Bio-Resources Yunnan University E.N.Pavlovsky Institute of Zoology and Parasitology Academy of Sciences of Republic of Tajikistan Research Center for Ecology and Environment of Central Asia Chinese Academy of Sciences Key Laboratory of Biogeography and Bioresource in Arid Land Xinjiang Institute of Ecology and GeographyChinese Academy of Sciences Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyChinese Academy of Sciences Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences

Tajik highlanders have resided in Pamirs（altitude ＞3000 m） for thousands of years. How they adapted to the harsh conditions remains unsolved. Herein, we measured four physiological traits for two groups of Tajik highlanders: Sarikoli Tajik and Wakhi Tajik, in comparison to highland Kyrgyz. Neither hematologic nor spirometric evidence exhibit Tibetan-style adaptation in Tajik highlanders. To discern the potential genetic mechanism of high altitude adaptation in Tajik highlanders, we sequenced the whole genomes of 19 Sarikoli Tajiks, 20 Wakhi Tajiks, and 20 lowland Tajiks. The population genomic analyses reveal Tajiks as a branch of Central Asians experiencing complicated patterns of gene flows from European, South Asian, and East Asian populations. The settlement of Pamirs by the Sarikoli Tajiks is approximated to be around 3200 years ago. Selective sweep assessments, verified by coalescent simulation, identify SNRNP40, ZCCHC17, and FABP3 on chromosome 1, and KCTD13, TMEM219, and TAoK2 on chromosome 16, as the potential targets of selection in the Sarikoli Tajiks. These genes are involved in neurological development and diseases. The selective haplotypes in the Sarikoli Tajiks are standing variations with distribution in lowland populations. Luciferase reporter assays show that the selective haplotype of KCTD13 promoter and enhancer regions exhibit higher transcription activity than the nonselective haplotype. overall, our findings provide novel insight into a very recent high altitude adaptation in humans, which probably targeted nervous system by utilizing standing variations via soft sweep.

关键词： High altitude adaptation Tajiks Pamirs nervous system KCTD13

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Animal Nutrition 2022年第2期9卷 143-158页

作者： Xing Lu Dong-Fang Deng Fei Huang Fabio Casu Emma Kraco Ryan J.newton Merry Zohn Swee J.Teh Aaron M.Watson Brian Shepherd Ying Ma Mahmound A.o.Dawood Lorena M.Rios Mendoza School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA South Carolina Department of Natural Resources CharlestonSC 29412USA USDA/ARS/School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA School of Veterinary Medicine Department of AnatomyPhysiologyand Cell BiologyUniversity of CaliforniaDavisCA 95616USA Department of Natural Sciences Marine Resources Research InstituteUniversity of WisconsinSuperiorWI 54880USA Yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhanChina Fisheries College Jimei UniversityXiamenChina. Faculty of Agriculture Kafrelsheikh UniversityKafr El-SheikhEgypt.

Microplastics are emergent contaminants threatening aquatic organisms including aquacultured fish.This study investigated the effects of high-density polyethylene(HDPE,100 to 125 mm)on yellow perch(Perca flavescens)based on integrative evaluation including growth performance,nutritional status,nutrient metabolism,fish health,and gut microbial community.Five test diets(0,1,2,4,or 8 g HDPE/100 g diet)containing 41%protein and 10.5%lipid were fed to juvenile perch(average body weight,25.9±0.2 g;n=15)at a feeding rate of 1.5%to 2.0%body weight daily.The feeding trial was conducted in a flow-through water system for 9 wk with 3 tanks per treatment and 15 yellow perch per tank.No mortality or HDPE accumulation in the fish was found in any treatments.Weight gain and condition factor of fish were not significantly impacted by HDPE(P>0.05).Compared to the control group,fish fed the 8%HDPE diet had significantly decreased levels of protein and ash(Ponse to the increasing levels of HDPE exposure,the hepatosomatic index value,hepatocyte size,and liver glycogen level were increased,but lipid content was reduced in the liver tissues.Compared to the control treatment,fish fed the 8%HDPE diet had significant accumulations of total bile acids and different metabolism pathways such as bile acid biosynthesis,pyruvate metabolism,and carnitine synthesis.Significant enterocyte necrosis was documented in the foregut of fish fed the 2%or 8%HDPE diet;and significant cell sloughing was observed in the midgut and hindgut of fish fed the 8%HDPE diet.Fish fed the 2%HDPE diet harbored different microbiota communities compared to the control fish.This study demonstrates that HDPE ranging from 100 to 125 mm in feed can be evacuated by yellow perch with no impact on growth.However,dietary exposure to HDPE decreased whole fish nutrition quality,altered nutrient metabolism and the intestinal histopathology as well as microbiota community of yellow perch.The results indicate that extended exposure may pose a risk to fish health and je

关键词： Dietary exposure Intestinal microbiota Liver metabolomics Microplastics Nutrient composition Yellow perch

HPV Vaccine: Integrative Review of National and International Guidelines

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Health 2022年第12期14卷 1287-1298页

作者： Durval Silva Tavares Filho Camille Marcele Resende Rocha Elizabeth Bacha Marcela Curvello Beltrão Lusitânia Maria de Barros Ádamo newton Marinho Andrade Fabiana Torres Valadares Yasmin Nunes Machado Centro Universitário Tiradentes (UNIT) Maceió Brasil

HPV (Human Papillomavirus) is an adenovirus of the Papillomaviridae family with approximately 200 subtypes (subdivided into low and high oncogenic risk groups), responsible for almost 99% of cervical cancers. Worldwide, there are more than 570,000 new cases and more than 311,000 women die each year from cervical cancer;for Brazil, the estimate is of more than 16,000 new cases per year. It is known that cervical cancer is one of the most easily preventable forms of cancer, as there is a highly effective vaccine against HPV. However, the morbidity and mortality rates correlated with human papillomavirus are still concerning, especially in less developed countries. In 2020, the WHo (World Health organization) presented three goals to achieve by 2030 the worldwide extermination of cervical cancer;among these goals is vaccination. Is the world preparing for this battle? The present work is an integrative review, comparing national and international guidelines for HPV vaccination published in the last five years in the consulted databases, using the descriptors “cervical cancer”, “prevention” and “guideline” and the boolean operator “and”, from which nine articles were selected. The study made it possible to compare international and national guidelines for vaccination against HPV, showing that developed countries implemented the HPV vaccine longer ago and follow the WHo recommendations more rigorously and effectively than underdeveloped or developing countries. Adopting a single dose as a strategy, as suggested by the WHo in April 2022, could be an important step towards increasing coverage and providing protection for a greater number of girls who do not have access to HPV immunization.

关键词： Immunization Prevention Guidelines HPV Cervical Cancer

An antiferromagnetic {Mn_8} ring supported by planar multidentate ligands

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Science China Chemistry 2012年第6期55卷 973-977页

作者： newton Graham N. SHIGA Takuya oSHIo Hiroki Graduate School of Pure and Applied Sciences Department of Chemistry University of Tsukuba Tennodai 1-1-1 Tsukuba 305-8571 Japan

Complexation of the planar multidentate ligand 3,5-bis-(2-hydroxyphenyl)pyrazole (H 3 L) with manganese chloride leads to the formation of the polynuclear complex [Mn Ⅲ 8 L 4 o 4 (Meo) 4 (MeoH) 8 ] (1). 1 has an octanuclear macrocyclic core in which the MnⅢ ions are bridged by four L molecules to form a ring type structure. Antiferromagnetic interactions were shown to be operative between metal centers.

关键词：反铁磁相互作用多齿配体平面多核络合物羟基苯基环型结构金属中心 MEo