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Chronic exposure to high-density polyethylene microplastic through feeding alters the nutrient metabolism of juvenile yellow perch (Perca flavescens)

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Animal Nutrition 2022年第2期9卷 143-158页

作者： Xing Lu Dong-Fang Deng Fei Huang Fabio Casu Emma Kraco Ryan j.newton Merry Zohn Swee j.Teh Aaron M.Watson Brian Shepherd Ying Ma Mahmound A.O.Dawood Lorena M.Rios Mendoza School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA South Carolina Department of Natural Resources CharlestonSC 29412USA USDA/ARS/School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA School of Veterinary Medicine Department of AnatomyPhysiologyand Cell BiologyUniversity of CaliforniaDavisCA 95616USA Department of Natural Sciences Marine Resources Research InstituteUniversity of WisconsinSuperiorWI 54880USA Yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhanChina Fisheries College Jimei UniversityXiamenChina. Faculty of Agriculture Kafrelsheikh UniversityKafr El-SheikhEgypt.

Microplastics are emergent contaminants threatening aquatic organisms including aquacultured *** study investigated the effects of high-density polyethylene(HDPE,100 to 125 mm)on yellow perch(Perca flavescens)based on integrative evaluation including growth performance,nutritional status,nutrient metabolism,fish health,and gut microbial *** test diets(0,1,2,4,or 8 g HDPE/100 g diet)containing 41%protein and 10.5%lipid were fed to juvenile perch(average body weight,25.9±0.2 g;n=15)at a feeding rate of 1.5%to 2.0%body weight *** feeding trial was conducted in a flow-through water system for 9 wk with 3 tanks per treatment and 15 yellow perch per *** mortality or HDPE accumulation in the fish was found in any *** gain and condition factor of fish were not significantly impacted by HDPE(P>0.05).Compared to the control group,fish fed the 8%HDPE diet had significantly decreased levels of protein and ash(P<0.05).In response to the increasing levels of HDPE exposure,the hepatosomatic index value,hepatocyte size,and liver glycogen level were increased,but lipid content was reduced in the liver *** to the control treatment,fish fed the 8%HDPE diet had significant accumulations of total bile acids and different metabolism pathways such as bile acid biosynthesis,pyruvate metabolism,and carnitine *** enterocyte necrosis was documented in the foregut of fish fed the 2%or 8%HDPE diet;and significant cell sloughing was observed in the midgut and hindgut of fish fed the 8%HDPE *** fed the 2%HDPE diet harbored different microbiota communities compared to the control *** study demonstrates that HDPE ranging from 100 to 125 mm in feed can be evacuated by yellow perch with no impact on ***,dietary exposure to HDPE decreased whole fish nutrition quality,altered nutrient metabolism and the intestinal histopathology as well as microbiota community of yellow *** results indicate that extende

关键词： Dietary exposure Intestinal microbiota Liver metabolomics Microplastics Nutrient composition Yellow perch

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NON-DESTRUCTIVE ANALYSIS OF THE NUCLEI OF TRANSGENIC LIVING CELLS USING LASER TWEEZERS AND NEAR-INFRARED RAMAN SPECTROSCOPIC TECHNIQUE

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Genomics, Proteomics & Bioinformatics 2005年第3期3卷 169-178页

作者： Wei Tang Ronald j. newton Chang-An Xie Yong-Qing Li Nicki Whitley Department of Biology Howell Science Complex East Carolina UniversityGreenville NC 27858-4353 USA. Department of Physics Howell Science Complex East Carolina University Greenville NC 27858-4353 USA.

Transgenic cell lines of loblolly pine （Pinus taeda L.） were analyzed by a compact laser-tweezers-Raman-spectroscopy （LTRS） system in this investigation. A low power diode laser at 785 nm was used for both laser optical trapping of single transgenic cells and excitation for near-infrared Raman spectroscopy of the nuclei of synchronized cells, which were treated as single organic particles, at the S-phase of the cell cycle. Transgenic living cells with gfp and uidA genes were used as biological samples to test this LTRS technique. As expected, different Raman spectra were observed from the tested biological samples. This technique provides a high sensitivity and enables real-time spectroscopic measurements of transgenic cell lines. It could be a valuable tool for the study of the fundamental cell and molecular biological process by trapping single nucleus and by providing a wealth of molecular information about the nuclei of cells.

关键词： transgenic cell laser optical trapping near-infrared Raman spectroscopy biological sample

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Network analyses:Inhibition of androgen receptor signaling reduces inflammation in the lung through AR-MAF-IL6 signaling axes

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Genes & Diseases 2024年第3期11卷 140-143页

作者： Albert R.Wang Andrew M.Baschnagel Zijian Ni Sean R.Brennan Hypatia K.newton Darya Buehler Christina Kendziorski Randall j.Kimple Gopal lyer Department of Human Oncology University of Wisconsin School of Medicine and Public HealthUniversity of WisconsinMadisonWI 53705USA Department of Biomedical Engineering University of Wisconsin-MadisonMadisonWI 53705USA University of Wisconsin Carbone Cancer Center MadisonWI 53705USA Department of Biostatistics and Medical Informatics University of Wisconsin-MadisonMadisonWI 53706USA Department of Biology Tufts UniversityMedfordMA 02155USA Department of Pathology and Laboratory Medicine University of Wisconsin School of Medicine and Public HealthUniversity of WisconsinMadisonWI 53705USA

Androgen receptor(AR)is a major transcription factor that plays a role in inflammatory response including interleukin-6(IL6)signaling.1 While AR regulation through paracrine loop signaling in prostate tissue is well-studied,its impact through an IL6 autocrine loop in the lung has not been wellstudied despite the organ's response to respiratory viral *** inhibition and RNA knockdown of AR identified a bZIP transcription factor MAF to be a common target of inflammation using these perturbations in lung cells.

关键词： inflammation lung respiratory

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长效避孕法

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国外医学（计划生育分册） 1991年第2期10卷 102-103,95页

作者： newton j 左学才

过去十年来,对长效方法的研究多在阴道环和埋植剂等。许多新方法中甾体剂量减低而且为零级释放率,因而可把口服避孕药的脉冲效应减小至最低限度。目前的WHO研究规划将继续研制一月一次的避孕针剂,3个月一次的孕激素注射剂,左旋18-甲基... 详细信息

过去十年来,对长效方法的研究多在阴道环和埋植剂等。许多新方法中甾体剂量减低而且为零级释放率,因而可把口服避孕药的脉冲效应减小至最低限度。目前的WHO研究规划将继续研制一月一次的避孕针剂,3个月一次的孕激素注射剂,左旋18-甲基炔诺酮释放阴道环及美国国立儿童健康和人类发育研究所联合研制的生物降解埋植剂(Capronor)及产后避孕的新方法。

关键词：长效避孕法避孕

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抗生素对火炬松的器官发生植株再生的影响(英文)

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journal of Forestry Research 2003年第3期14卷 185-190页

作者： Latoya Harris Ronald j.newton Department of Biology Howell Science ComplexEast Carolina UniversityGreenvilleDepartment of BiologyHowell Science ComplexEast Carolina UniversityGreenville NC27858-4353USANC27858-4353USA

本文研究了氨苄青霉素、羧苄青霉素和头孢霉素等3种抗生素对火炬松愈伤组织的生长和分化及不定芽生根的影响。结果表明,头孢霉素最有利于愈伤组织的诱导和生长,羧苄青霉素最有利于芽的分化,氨苄青霉素降低了不定芽的生根频率。所有试验... 详细信息

本文研究了氨苄青霉素、羧苄青霉素和头孢霉素等3种抗生素对火炬松愈伤组织的生长和分化及不定芽生根的影响。结果表明,头孢霉素最有利于愈伤组织的诱导和生长,羧苄青霉素最有利于芽的分化,氨苄青霉素降低了不定芽的生根频率。所有试验的3种抗生素提高愈伤组织的形成和芽再生,但降低了芽的生根频率。这些结果表明,选择合适的抗生素对优化火炬松遗传转化体系有重要作用。图3表4参25。

关键词：火炬松抗生素器官发生植株再生愈伤组织诱导生长遗传转化

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植物生物技术:苏云金杆菌抗虫基因的研究和它对未来树木遗传工程的影响(英文)

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journal of Forestry Research 2004年第1期15卷 1-10,J001页

作者：唐巍 Latoya Harris Ronald j.newton Department of Biology Howell Science Complex East Carolina UniversityGreenville NC 27858-4353 USA Department of Biology Howell Science Complex East Carolina UniversityGreenvilleNC 27858-4353 USA

许多不同的技术可以促进农业生产力的持续增长,生物技术是其中最重要和最有前途的方法。因为生物技术不仅可以增加生产力,而且在环境和自然资源的保护中起重要作用,应用生物技术可以生产高产、优质、高抗性和无病植物,同时也可以提高育... 详细信息

许多不同的技术可以促进农业生产力的持续增长,生物技术是其中最重要和最有前途的方法。因为生物技术不仅可以增加生产力,而且在环境和自然资源的保护中起重要作用,应用生物技术可以生产高产、优质、高抗性和无病植物,同时也可以提高育种效率和促进食品的工业化生产。苏云金杆菌抗虫基因是目前生物技术领域中应用最广泛的例子之一,由此而产生的抗虫植物正在广泛应用于世界各地的商业化生产。本文综述了苏云金杆菌抗虫基因的研究进展和它在未来林木遗传工程中的应用。表4参68。

关键词：植物生物技术苏云金杆菌抗虫基因树木遗传工程抗虫性育种

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Chronic inflammation and achilles tendinopathy

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中华物理医学与康复杂志 2018年第3期40卷 173-173页

作者： Dakin SG newton j Martinez FO

BACKGROUND AND OBjECTIVE Recent studies have identified immune-component cells in biopsy specimens from patients with non-ruptured chronic Achilles *** study was designed to better understand the inflammatory component of this *** Subjects were 17 patients with chronic Achilles tendinopathy,presenting for high-volume injections(HVI),and 19 patients with Achilles tendon rupture,presenting for surgical *** from each group were compared with those of hamstring tendons

关键词：免疫力跟腱疾病患者治疗方法

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Patient, Provider and System Factors Impacting on the Diagnosis and Management of Lung Cancer Care in Australia

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journal of Cancer Therapy 2012年第4期3卷 406-411页

作者： Moyez jiwa Patricia M. Davidson Phillip j. newton Michelle L. Digiacomo Sarah j. McGrath Cornelius j. Lotriet Centre for Cardiovascular and Chronic Care Faculty of Health University of Technology Sydney Sydney Australia Curtin Health Innovation Research Institute Curtin University Perth Australia

Background: Lung cancer is the leading cause of cancer death in Australia, with only modest improvements in survival. This study aims to identify factors impacting on diagnosis and management of lung cancer with particular reference to Australian primary care. Methods: A sequential mixed method modified approach employing interview and a two-phased survey technique. Following telephonic interviews with 31 health professionals (individuals representing general practitioners, specialized physicians, nurses and allied health practitioners), interview data was analysed using qualitative thematic analysis, and surveys using descriptive statistics. Emergent themes were organised under patient, provider and system factors. Interviews ceased upon saturation of data. Results: Multiple patient, provider and systems issues were seen to contribute to adverse health outcomes. There is a strong relationship between smoking and outcomes, and factors related to higher smoking rates such as a lower socioeconomic status. For smokers, guilt and/or denial was considered a reason for delay in the decision to seek medical care for cough or shortness of breath. Aboriginal people under-report morbidity related to smoking and chronic obstructive pulmonary disease;other patients fail to recognise the significance of their symptoms. Discussion: Despite the poor prognosis of lung cancer diagnosis, increased awareness of presentation and treatment options can address disparities in health outcomes.

关键词： Lung Cancer Primary Care General Practice Survival Diagnosis

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与生长激素相关的高等植物细胞周期生化调控(英文)

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journal of Forestry Research 2004年第3期15卷 227-232页

作者：唐巍 Department of Biology Howell Science ComplexEast Carolina UniversityGreenvilleNC27858-4353USA

高等植物细胞周期是细胞生物学上的重要研究领域。细胞周期受遗传和发育影响因素的调控。本文综述高等植物细胞周期中涉及到细胞周期关卡GO-G1-S期和G2-M期的激素调控。最新研究成果证明玉米素、腺嘌呤、激动素、赤霉素和脱落酸等通过... 详细信息

高等植物细胞周期是细胞生物学上的重要研究领域。细胞周期受遗传和发育影响因素的调控。本文综述高等植物细胞周期中涉及到细胞周期关卡GO-G1-S期和G2-M期的激素调控。最新研究成果证明玉米素、腺嘌呤、激动素、赤霉素和脱落酸等通过细胞周期蛋白依赖性蛋白激酶及细胞周期关卡的脱磷酸化控制细胞周期。植物D-型细胞周期蛋白也已被证明在细胞周期调控中起关键作用。本文有关细胞周期的研究综述对植物细胞和发育生物学研究有参考价值。图3参61。

关键词：生物化学调控细胞周期高等植物发育细胞生物学

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发育中高粱颖果的可溶性碳水化合物

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国外农学-杂粮作物 1985年第3期 14-16页

作者： R.j.newton 廖衍伦张路平

植株的任何器官都能控制整个体内碳水化合物的分配,但最近的研究指出,最重要的是库容量,所以库特性对于了解作物的生产力是很重要的。若干研究者(Shannah,1988,1972;Beevers等,1969;Keener等,1979)根据蔗糖运输到库区及水解和代谢的情况... 详细信息

植株的任何器官都能控制整个体内碳水化合物的分配,但最近的研究指出,最重要的是库容量,所以库特性对于了解作物的生产力是很重要的。若干研究者(Shannah,1988,1972;Beevers等,1969;Keener等,1979)根据蔗糖运输到库区及水解和代谢的情况,确定了库代

关键词：颖果可溶性碳水化合物饲料营养成分高粱

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