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Molecular characterization and diversity analysis of bacterial communities associated with Dialeurolonga malleswaramensis （Hemiptera： Aleyrodidae） adults using 16S rDNA amplicon pyrosequencing and FISH

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Insect Science 2016年第5期23卷 704-711页

作者： neeti Pandey Raman Rajagopal Gut Biology Lab Room No 117 Department of Zoology University of Delhi Delhi 110007 India

Dialeurolonga malleswaramensis Sundararaj （Hemiptera： Aleyrodidae） is a phytophagous sap sucking insect. It infests Polyalthia longifolia, an important avenue tree of India, effective in alleviating noise pollution and having immense medicinal importance. Samples of this insect were collected from Polyalthia longifolia. The cytochrome c oxidase subunit I gene （mtCOl） helped in the molecular characterization of the insect. This study reports the bacterial diversity in D. malleswararnensis adults by high throughput 16S rDNA amplicon pyrosequencing. The major genera identified were Portiera and Arsenophonus. Other bacterial genera detected were uncultured alpha proteobacterium, Sphingopyxis and Methylobacterium. We also employed fluorescence in situ hybridization （FISH） in whole mount samples to confirm the presence of dominant endosymbionts Portiera and Arsenophonus to the bacteriocyte of D. malleswaramensis. This study concludes that combining techniques like 16S rDNA amplicon pyrosequencing and FISH reveal both dominant and rare bacteria. The data also predict the evolutionary position of this pest with respect to other whitefly species using a mitochondrial marker.

关键词： 16S rDNA amplicon pyrosequencing bacterial diversity Dialeurolongamalleswaramensis FISH mtC01

Review of Microplastic Pollution in the Environment and Emerging Recycling Solutions

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Journal of Renewable Materials 2019年第12期7卷 1251-1268页

作者： Gregory Reimonn Taofeng Lu neeti Gandhi Wan-Ting Chen Department of Plastic Engineering University of Massachusetts LowellLowellMA01854USA School of Chemical Engineering Purdue UniversityWest LafayetteIN47906USA.

Microplastic pollution represents a side-effect stemming from a global plastic waste mismanagement problem and includes degraded particles or mass produced plastic particles less than 5 mm in largest *** small nature of microplastics gives this area of pollution different environmental concerns than general plastic waste in the *** biological toxicity of particles,their internal components,and their surface level changes all present opportunities for these particles to adversely affect the environment around ***,it is necessary to review the current literature surrounding this topic and identify areas where the study of microplastic can be pushed *** we present current methods in studying microplastics,some of the ways by which microplastics affect the environment and attempt to shed light on how this research can *** addition,we review current recycling methods developing for the processing of mixed-plastic *** methods,including hydrothermal processing and solvent extraction,provide a unique opportunity to separate plastic waste and improve the viability of the plastics recycling industry.

关键词： Microplastic pollution additive toxicology marine plastic pollution plastic recycling sustainability

Fingerprint analysis for the determination of hand origin(right/left)using the axis slant in whorl patterns

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Forensic Sciences Research 2022年第2期7卷 285-289页

作者： neeti Kapoor Ashish Badiye Swati Dubey Mishra Department of Forensic Science Government Institute of Forensic ScienceNagpurMaharashtraIndia Shri Vaishnav Institute of Forensic Science Shri Vaishnav Vidyapeeth VishwavidyalayaIndoreMadhya PradeshIndia

Fingerprints are frequently encountered during both civil and criminal *** possess numerous characteristics that assist with personal *** the hand of origin(right or left)for an individual fingerprint would help reduce investigation time and potentially eliminate certain *** this study,we collected a total of 2900 single digit fingerprints from 290 individuals,and the whorl axis slant was examined in the 743 whorl pattern fingerprints(385 from the right hand and 358 from the left hand).A slant towards the right side was present in 81.82%of samples from the right hand,while a slant towards the left side was observed in 80.73%of samples from the left *** applying a chi-square test to the dataset,the results were found to be statistically significant for the determination of hand *** results suggest that the whorl axis slant in a fingerprint is indicative of hand origin(right or left).

关键词： Forensic sciences side determination whorl slant fingerprint analysis crime hand

Life Cycle Assessment of Recycling High-Density Polyethylene Plastic Waste

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Journal of Renewable Materials 2021年第8期9卷 1463-1483页

作者： neeti Gandhi Nicholas Farfaras Nien-Hwa Linda Wang Wan-Ting Chen Department of Chemical Engineering Virginia Polytechnic and State InstituteBlacksburg24061USA Department of Plastics Engineering University of Massachusetts LowellLowell01854USA School of Chemical Engineering Purdue UniversityWest Lafayette47907USA

Increasing production and use of various novel plastics products,a low recycling rate,and lack of effective recycling/disposal methods have resulted in an exponential growth in plastic waste accumulation in landfills and in the *** better understand the effects of plastic waste,Life Cycle Analysis(LCA)was done to compare the effects of various production and disposal *** shows the specific effects of the cradle-to-grave or cradle-to-cradle scenarios for landfill,incineration,and mechanical *** analysis clearly indicates that increasing recycling of plastics can significantly save energy and eliminate harmful emissions of various carcinogens and GHGs into the *** recycling increases,the need for virgin-plastic production can be greatly ***,the results of this study may help improve current mechanical recycling processes as well as potential future recycling methods,such as chemical *** about the current recycling/disposal methods for plastics have brought increasing attention to the waste accumulation ***,with the current COVID-19 pandemic,plastic accumulation is expected to increase significantly in the near future.A better understanding of the quantitative effects of the various disposal methods can help guide policies and future research toward effective solutions of the plastic waste problem.

关键词： Life Cycle Analysis(LCA) mechanical recycling microplastic chemical recycling plastic policy

Physicochemical and in Vitro Antibacterial Evaluation of Metronidazole Loaded Eudragit S‑100 Nanofbrous Mats for the Intestinal Drug Delivery

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Advanced Fiber Materials 2022年第1期4卷 76-88页

作者： Priyanka Prakash Rade Prabhanjan Shridhar Giram Ajinkya A.Shitole neeti Sharma Baijayantimala Garnaik Polymer Science and Engineering Division CSIR-National Chemical LaboratoryPune 411008India Academy of Scientifc and Innovative Research(AcSIR) Ghaziabad 201002India Symbiosis School of Biological Sciences Symbiosis International(Deemed University)Pune 412115India

Metronidazole(MTZ)loaded Eudragit S-100(ES-100)nonwoven nanofbrous mats were successfully electrospun and evaluated for intestinal drug *** was varied in the range of 1–15%(w/w)in ES-100 nanofbrous mats,the morphological characterization of nanofbrous mats was carried out using FE-SEM and the average diameter of nanofber was found in the range 150–600 *** and DSC demonstrated the amorphous nature of MTZ in ES-100 nanofbrous *** contact angle analysis confrmed the hydrophobic *** mechanical strength of ES-100 nanofbrous mats decreased with increasing MTZ *** drug release profles showed 74%MTZ release from ES-100d within 2 h at pH 6.8 which is the colonic *** activities against gram-positive bacteria(Staphylococcus aureus)and gramnegative bacteria(Escherichia coli)showed that the ES-100 nanofbrous mats loaded with MTZ exhibited good activity.

关键词： ES-100 Metronidazole Nanofbers Antibacterial Intestinal drug delivery

Mapping the Expression Profiles of Grain Yield QTL Associated miRNAs in Rice Varieties with Different Grain Size

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American Journal of Plant Sciences 2022年第9期13卷 1261-1281页

作者： Sudhir Kumar neeti Sanan-Mishra Plant RNAi Biology Group International Center for Genetic Engineering and Biotechnology Aruna Asaf Ali Marg New Delhi India

microRNAs (miRNAs) are key regulatory molecules that fine tune gene expression at the transcriptional and post-transcriptional levels through the RNA silencing pathways. They play an important role in regulating plant growth, development and response to a/biotic stress conditions. Grain yield is a complex trait that is governed by the coordinated action of several genetic and environmental factors. A number of genes and miRNAs have been identified to affect the grain productivity and yield. In this study, we identified the miRNAs that map to grain yield QTLs in rice. The expression variations of these miRNAs and their target transcript were studied across different tissues of three indica rice varieties with different grain morphology. The varieties used include the extra-long and slender grained Pusa Basmati 1 (PB1), medium grain sized IR64 and the short grained Pokkali (PK). The windows for miRNA target correlation were captured and their putative role in regulating rice grain yield is discussed.

关键词： miRNA Grain Yield Expression Analysis Rice IR64 Basmati Pokkali

Comparative Analysis of RNAi Suppression Activity of Proteins from Two Disparate Viruses

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American Journal of Plant Sciences 2014年第12期5卷 1789-1798页

作者： Sudhanshu Sekhar Das neeti Sanan-Mishra Plant Molecular Biology Group International Centre for Genetic Engineering and Biotechnology New Delhi India

RNAi is an efficient surveillance machinery that plays a robust defensive role in shielding plant and animal hosts against viral infections. In counter-defense viruses encode suppressor proteins that have the ability to restrict the RNAi machinery to ensure successful systemic invasion. The B2 protein of insect Flock House Virus (FHV-B2) and AC2 protein of Mungbean Yellow Mosaic India Virus (MYMIV-AC2) are two well-characterized suppressors of RNAi, capable of reversing reporter gene silencing. In this study, we compared the strength of the two suppressors by assaying for the degree of RNAi reversion and the duration of sustaining the reversal in planta. The suppression activity was observed by assaying for GFP fluorescence at 3 dpi, 7 dpi and 14 dpi. The phenotypic observations were corroborated with small RNA Northern Blotting and semi-quantitative RT-PCR. The results indicate that suppressor strength of FHVB2 is comparable to MYMIV-AC2, although they are encoded by virus infecting host from two different eukaryotic kingdoms. This study will provide new insights to dissect the conservation in the RNAi pathways during the host-virus interactions.

关键词： MYMIV-AC2 FHVB2 Agroinfiltration RNA Silencing Suppressor (RSS) Reversal of GFP Silencing Assay

Protocol for Artificial MicroRNA Mediated Over-Expression of miR820 in Indica Rice

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American Journal of Plant Sciences 2015年第12期6卷 1951-1961页

作者： Neha Sharma Sandeep Panchal neeti Sanan-Mishra Plant Molecular Biology Group International Center for Genetic Engineering and Biotechnology New Delhi India

In the present study, we illustrate the strategy and protocol required to generate rice transgenics over-expressing the 21-nt form of Osa-miR820. The miR exists in two size variants of 21-nt and 24-nt so the natural precursor cannot be employed for the purpose of miR over-expression as the cellular machinery can process both size variants thereby masking the role of PTGS regulation. Hence, we adopted the artificial miR technology to specifically over-express the 21-nt species in the transgenics. During the course of experiments it was observed that the amiR constructs probably interfered with the regeneration of the transformed callus, necessitating protocol modifications. The results indicate the successful over-expression of the 21-nt miR species. These plants can serve as a useful source for the functional dissection of the role played by the 21-nt Osa-miR820 species. They will also be valuable in highlighting the importance for the existence of a dual mode of miR mediated target regulation.

关键词： Osa-miR820 Over-Expression Agrobacterium Artificial microRNA (amiR)

Effect of PAIP1 on the metastatic potential and prognostic significance in oral squamous cell carcinoma

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International Journal of Oral Science 2022年第1期14卷 113-122页

作者： neeti Swarup Kyoung-Ok Hong Kunal Chawla Su-Jung Choi Ji-Ae Shin Kyu-Young Oh Hye-Jung Yoon Jae-Il Lee Sung-Dae Cho Seong-Doo Hong Department of Oral Pathology School of Dentistry and Dental Research InstituteSeoul National UniversitySeoulRepublic of Korea Department of Computer Science School of Interactive ComputingGeorgia Institute of TechnologyAtlantaGAUSA

Poly Adenylate Binding Protein Interacting protein 1(PAIP1) plays a critical role in translation initiation and is associated with the several cancer types. However, its function and clinical significance have not yet been described in oral squamous cell carcinoma(OSCC) and its associated features like lymph node metastasis(LNM). Here, we used the data available from Gene Expression Omnibus(GEO), The Cancer Genome Atlas(TCGA), and Clinical Proteomic Tumor Analysis Consortium(CPTAC) to analyze PAIP1 expression in oral cancer. The publicly available data suggests that PAIP1 mRNA and protein levels were increased in OSCC. The high PAIP1 expression was more evident in samples with advanced stage, LNM, and worse pattern of invasion. Moreover, the in vitro experiments revealed that PAIP1 knockdown attenuated colony forming, the aggressiveness of OSCC cell lines, decreasing MMP9 activity and SRC phosphorylation. Importantly, we found a correlation between PAIP1 and pSRC through the analysis of the IHC scores and CPTAC data in patient samples. Our findings suggest that PAIP1 could be an independent prognostic factor in OSCC with LNM and a suitable therapeutic target to improve OSCC patient outcomes.

关键词： squamous invasion metastasis