Protocol for Artificial MicroRNA Mediated Over-Expression of miR820 in Indica Rice

作     者：Neha Sharma Sandeep Panchal Neeti Sanan-Mishra 

作者机构：Plant Molecular Biology Group International Center for Genetic Engineering and Biotechnology New Delhi India 

出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)

年 卷 期：2015年第6卷第12期

页      面：1951-1961页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Osa-miR820 Over-Expression Agrobacterium Artificial microRNA (amiR) 

摘      要：In the present study, we illustrate the strategy and protocol required to generate rice transgenics over-expressing the 21-nt form of Osa-miR820. The miR exists in two size variants of 21-nt and 24-nt so the natural precursor cannot be employed for the purpose of miR over-expression as the cellular machinery can process both size variants thereby masking the role of PTGS regulation. Hence, we adopted the artificial miR technology to specifically over-express the 21-nt species in the transgenics. During the course of experiments it was observed that the amiR constructs probably interfered with the regeneration of the transformed callus, necessitating protocol modifications. The results indicate the successful over-expression of the 21-nt miR species. These plants can serve as a useful source for the functional dissection of the role played by the 21-nt Osa-miR820 species. They will also be valuable in highlighting the importance for the existence of a dual mode of miR mediated target regulation.