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Detection of H pylori infection by ELISA and Western blot techniques and evaluation of anti CagA seropositivity in adult Turkish dyspeptic patients

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World Journal of Gastroenterology 2006年第33期12卷 5375-5378页

作者： zlem Yilmaz nazime ■en Ahmet Ali Küpelio■lu kay ■im■ek Department of Microbiology and Clinical Microbiology Faculty of Medicine Dokuz Eylül University Inciralti-35340 Izmir Turkey Department of Pathology Faculty of Medicine Dokuz Eylül University Inciralti-35340 Izmir Turkey Department of Gastroenterology Faculty of Medicine Dokuz Eylül University Inciralti-35340 Izmir Turkey

AIM: To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients. METHODS: We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay (ELISA) and Western blot in dyspeptic Turkish patients. H pylori status was determined by histology and rapid urease testing. RESULTS: Fifty-six patients were entered. Forty-eight (85.7%) out of the 56 patients were positive for H pylori. H pylori IgG seropositivity was 82.1%, IgA seropositivity 48.2%. CagA ELISA showed that IgG was positive in 50% and IgA in 30.4% of those with H pylori infections. Western blot showed that IgG seropositivity was 80.4% and IgA seropositivity 33.9%. Western blot detected IgG antibodies with reactivity to CagA in 50%, VacA in 62.5%, UreB in 87.5%, UreA in 80.4%, and OMP in 57.1%. None of the tests had a sensitivity and specifi city above 80%. CONCLUSION: None of these commercial tests seems clinically useful for H pylori detection in adult dyspeptic patients, while Western blot can give seropositivity and determine anti-CagA, VacA virulence factor status of Turkish dyspeptic patients in the Izmir region.

关键词： H pylori, Serum CagA, enzyme-linked immunoassay Western blot Serodiagnosis Dyspepticpatients

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