Detection of H pylori infection by ELISA and Western blot techniques and evaluation of anti CagA seropositivity in adult Turkish dyspeptic patients

作     者：zlem Yilmaz Nazime ■en Ahmet Ali Küpelio■lu kay ■im■ek 

作者机构：Department of Microbiology and Clinical Microbiology Faculty of Medicine Dokuz Eylül University Inciralti-35340 Izmir Turkey Department of Pathology Faculty of Medicine Dokuz Eylül University Inciralti-35340 Izmir Turkey Department of Gastroenterology Faculty of Medicine Dokuz Eylül University Inciralti-35340 Izmir Turkey 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第33期

页      面：5375-5378页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Dokuz Eylül University Research Foundation grant 2002 02.KB.SA■.024 

主　　题：H pylori, Serum CagA, Enzyme-linked immunoassay Western blot Serodiagnosis Dyspepticpatients 

摘      要：AIM: To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients. METHODS: We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay (ELISA) and Western blot in dyspeptic Turkish patients. H pylori status was determined by histology and rapid urease testing. RESULTS: Fifty-six patients were entered. Forty-eight (85.7%) out of the 56 patients were positive for H pylori. H pylori IgG seropositivity was 82.1%, IgA seropositivity 48.2%. CagA ELISA showed that IgG was positive in 50% and IgA in 30.4% of those with H pylori infections. Western blot showed that IgG seropositivity was 80.4% and IgA seropositivity 33.9%. Western blot detected IgG antibodies with reactivity to CagA in 50%, VacA in 62.5%, UreB in 87.5%, UreA in 80.4%, and OMP in 57.1%. None of the tests had a sensitivity and specifi city above 80%. CONCLUSION: None of these commercial tests seems clinically useful for H pylori detection in adult dyspeptic patients, while Western blot can give seropositivity and determine anti-CagA, VacA virulence factor status of Turkish dyspeptic patients in the Izmir region.