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Enhancement of the Antigenotoxic and Antioxidant Actions of Eugenol from Spice Clove and the Stabilizer Gum Arabic on Colorectal Carcinogenesis

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Food and Nutrition Sciences 2024年第1期15卷 71-100页

作者： nayanna de oliveira ramos melo Lucas Gabriel da Costa Marques Humberto Maia Costa Neto Matheus de Sousa Silva Francisco Vagnaldo Fechine Jamacaru Bruno Coêlho Cavalcanti Antônio Adailson de Sousa Silva Conceição Aparecida Dornelas Postgraduate Program in Medical-Surgical Sciences School of Medicine Federal University of Ceará Fortaleza Brazil School of Medicine Federal University of Ceará Fortaleza Brazil Nucleus of Research and development of Medicines (NPDM) Laboratory of Pharmacology and Preclinical Research School of Medicine Federal University of Ceará Fortaleza Brazil Nucleus for Research and development of Medicines (NPDM) National Laboratory of Experimental Oncology Federal University of Ceará Fortaleza Brazil Postgraduate Program in Pathology and Medical-Surgical Sciences School of Medicine Federal University of Ceará Fortaleza Brazil

Spices are defined as any aromatic condiment of plant origin used to alter the flavor and aroma of foods. Besides flavor and aroma, many spices have antioxidant activity, mainly related to the presence in cloves of phenolic compounds, such as flavonoids, terpenoids and eugenol. In turn, the most common uses of gum arabic are in the form of powder for addition to soft drink syrups, cuisine and baked goods, specifically to stabilize the texture of products, increase the viscosity of liquids and promote the leavening of baked products (e.g., cakes). Both eugenol, extracted from cloves, and gum arabic, extracted from the hardened sap of two species of the Acacia tree, are dietary constituents routinely consumed virtually throughout the world. Both of them are also widely used medicinally to inhibit oxidative stress and genotoxicity. The prevention arm of the study included groups: Ia, IIa, IIIa, Iva, V, VI, VII, VIII. Once a week for 20 weeks, the controls received saline s.c. while the experimental groups received DMH at 20 mg/kg s.c. During the same period and for an additional 9 weeks, the animals received either water, 10% GA, EUG, or 10% GA + EUG by gavage. The treatment arm of the study included groups Ib, IIb, IIIb e IVb, IX, X, XI, XII). Once a week for 20 weeks, the controls received saline s.c. while the experimental groups received DMH at 20 mg/kg s.c. During the subsequent 9 weeks, the animals received either water, 10% GA, EUG or 10% GA + EUG by gavage. The novelty of this study is the investigation of their use alone and together for the prevention and treatment of experimental colorectal carcinogenesis induced by dimethylhydrazine. Our results show that the combined use of 10% gum arabic and eugenol was effective, with antioxidant action in the colon, as well as reducing oxidative stress in all colon segments and preventing and treating genotoxicity in all colon segments. Furthermore, their joint administration reduced the number of aberrant crypts and th

关键词： Eugenol Gum Arabic Carcinogenesis Oxidative Stress Genotoxicity

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Influence of broken kernels content on soybean quality during storage

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Grain & Oil Science and Technology 2024年第2期7卷 105-112页

作者： Lazaro da Costa Correa Canizares Cesar Augusto Gaioso Newiton da Silva Timm Silvia Leticia Rivero Meza Adriano Hirsch ramos Maurício de oliveira Everton Lutz Moacir Cardoso Elias department of Agroindustry Science and Technology Federal University of PelotasPelotasRio Grande do Sul 96010-900Brazil Information and Communication Technological Axis Federal Institute of EducationScienceand Technology FarroupilhaCampus PanambiPanambiRio Grande do Sul 98280-000Brazil

Although it is recognized that the post-harvest system is most responsible for the loss of soybean quality,the real impact of this loss is still *** regulation allows 15%and 30%of broken soybean for group I and group II(quality groups),***,the industry is not informed about the loss in the quality parameters of soybeans and its impacts during long-term ***,the objective was to evaluate the effect of the breakage kernel percentage of soybean stored for 12 *** of 15% of breakage kernels did not affect soybean ***,content of 30% of breakage kernels affected significantly soybean quality,which was evidenced by the increase of up to 75% in moldy soybeans,72% in acidity,50% in leached solids,27% in electrical conductivity,and the decrease of up to 12% in soluble protein,6.4% in germination and 3.5% in thousand kernel weight after 8 months of *** the legislation establishes a percentage limit,it is recommended to store soybeans with up to 15% breakage *** the contrary,values higher than that can cause a significant reduction in soybean quality,resulting in commercial losses.

关键词： Soybean quality Breakage kernels Storage problems Grain defects Quality parameters

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Histological Assessment of Bone Regeneration in the Maxilla with Homologous Bone Graft:A Feasible Option for Maxillary Bone Reconstruction

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Journal of Renewable Materials 2024年第1期12卷 131-148页

作者： Sergio Henrique Gonçalves Motta Ana Paula ramos Soares Juliana Campos Hasse Fernandes Gustavo Vicentis oliveira Fernandes Graduate Center of Faculdade Redentor CLIVORio de JaneiroBrazil Periodontics and Oral Medicine department University of Michigan School of DentistryAnn ArborMI48109USA Centre for Interdisciplinary Research in Health(CIIS) Universidade Católica PortuguesaViseu3504-505Portugal

Bone biomaterials have been increasingly used to reconstruct maxillary atrophic ***,the aim of this study was to evaluate bone reconstruction in the maxilla using a homologous cortico-cancellous FFB(lyophilized)graft and verify its *** individuals were included from 2014 to *** first surgery was performed to install homologous bone blocks in the *** period of the second intervention varied between 5 months and 15 days to 11 months(≈7.93 months).The biopsies were taken from the central region of the matured graft during the surgery for implant *** patients presented clinical and radiographic conditions for the installation of dental *** was a 100%of survival *** histological assessment showed that the homologous block bone graft was an osteoconductive biomaterial,with connective tissue present,and newly formed bone juxtaposed on its *** were bone trabeculae with osteocytes and active osteoblasts with connective tissue in the mineralization process;the remodeling process can be found through the reverse lines.A limited focus of necrosis with fibrosis was detected,with small resorption and areas of inflammatory infiltrate,but without clinical *** homologous block bone graft can be considered a feasible option to substitute the autogenous bone graft(gold standard),with predictable clinical and favorable histological *** patients had a shorter surgical period,low morbidity,and an unlimited amount of biomaterial available at an accessible cost.

关键词： Regeneration bone graft histology homologous allogenous autogenous

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Possibilities of using silicate rock powder:An overview

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Geoscience Frontiers 2022年第1期13卷 446-456页

作者： Claudete Gindri ramos James C.Hower Erika Blanco Marcos Leandro Silva oliveira Suzi Huff Theodoro department of Civil and Environmental Engineering Universidad de la CostaCalle 58#55-66BarranquillaColombia departamento de Ingeniería Civil y Arquitectura Universidad de LimaAvenida Javier Prado Este 4600Santiago de Surco 1503Peru Center for Applied Energy Research University of Kentucky2540 Research Park DriveLexingtonKY 40511USA department of Earth&Environmental Sciences University of KentuckyLexingtonKY 40511USA Postgraduate Program of Environment and Rural development(PPG-MAdeR) Área Universitária01Vila Nossa Senhora de Fátima–Campus UnBPlanaltinaDFBrazil

This study evaluates the on use of crushed rocks(remineralizers)to increase soil fertility levels and which contributed to increase agricultural productivity,recovery of degraded areas,decontamination of water,and carbon *** use of these geological materials is part of the assumptions of rock technology and,indirectly,facilitates the achievement of sustainable development goals related to soil management,climate change,and the preservation of water *** over the past 50 years on silicate rocks focused on soil fertility management and agricultural *** recently,the combined use with microorganisms and organic correctives have shown positive results to mitigate soil degradation;to expand carbon sequestration and storage;and to contribute to the adsorption of contaminants from water and *** this article we show results obtained in several countries and we show that this technology can contribute to the sustainability of agriculture,as well as to reverse global *** mineral nutrients are released more slowly from these types of inputs,they remain in the soil for a longer time,stimulating the soil *** addition,they are a technology to soluble synthetic fertilizers replace,since the few nutrients derived from such inputs not consumed by plants are lost by leaching,contaminating groundwater and water *** addition,conventional methods rely heavily on chemical pesticides which cause damage to soil’s microfauna(responsible for the decomposition of organic matter and nutrient cycling)and the loss of organic carbon(in the form of dioxide),which is quickly dispersed in the *** rock powders are applied in natura,have long-lasting residual effects and reduce greenhouse gas emissions.

关键词： Rock powder Soil management Carbon storage Water contaminant adsorption

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Pd-TiO_(2)/C催化剂中TiO_(2)对直接碱性燃料电池中丙三醇氧化的影响

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燃料化学学报 2022年第4期50卷 474-483页

作者： Viviane Santos Pereira Júlio Nandenha Andrezza ramos Almir oliveira Neto Instituto de Pesquisas Energéticas e Nucleares-IPEN-CNEN/SP Centro de Células a Combustível e Hidrogênio-CECCO Cidade UniversitáriaSão Paulo-SP 05508-000Brasil

以Vulcan XC-72炭为载体,采用硼氢化钠还原法制备了Pd-TiO_(2)/C电催化剂用于直接丙三醇燃料电池阳极材料,并用能量色散谱(EDX)、X射线衍射(XRD)、透射电子显微镜(TEM)、循环伏安法(CV)、计时电流法(I-t)和全反射-傅里叶变换红外光谱(AT... 详细信息

以Vulcan XC-72炭为载体,采用硼氢化钠还原法制备了Pd-TiO_(2)/C电催化剂用于直接丙三醇燃料电池阳极材料,并用能量色散谱(EDX)、X射线衍射(XRD)、透射电子显微镜(TEM)、循环伏安法(CV)、计时电流法(I-t)和全反射-傅里叶变换红外光谱(ATR-FTIR)对催化剂进行了表征。EDX结果表明,Pd-TiO_(2)/C中Pd和Ti含量接近名义原子比。Pd-TiO_(2)的X射线衍射结果表明,Pd具有面心立方fcc结构,同时TiO_(2)展现出四方结构的锐钛矿相的峰特征。TEM图像显示Pd和TiO_(2)纳米颗粒在碳载体中分布均匀并存在一些簇状区域,纳米颗粒大小为7.0-8.0 nm。循环伏安显示在1 mol/L KOH电解液中催化剂Pd-TiO_(2)/C在约-0.7 V vs Ag/AgCl有明显的析氢行为和电容增加现象,表明催化剂在TiO_(2)修饰后活性明显增加。通过循环伏安对碱性体系的丙三醇电催化氧化表明,Pd-TiO_(2)/C(70∶30)显示了非常好的催化活性:起始氧化电位也变得更负达到-0.4V vs Ag/AgCl,氧化电流达到14.91 mA/cm,优于Pd-TiO_(2)/C(90∶10)的9.37 mA/cm和Pd-TiO_(2)/C(50∶50)的4.88 mA/cm,明显高于Pd/C和TiO_(2)/C的1.88和0.55 mA/cm。I-t实验表明1000 s之前Pd-TiO_(2)/C展现出最高的电流密度,但在1000 s后电流密度有一定程度的下降,表明催化剂的催化活性很高但长期稳定性方面需要进一步提升。在碱性直接丙三醇燃料电池中进行的实验表明,所制备的Pd-TiO_(2)/C(50∶50)、Pd-TiO_(2)/C(70∶30)和Pd-TiO_(2)/C(90∶10)对甘油的电化学氧化性能均优于Pd/C电催化剂,其中,Pd-TiO_(2)/C(70∶30)的催化活性最好:开路电压达到817 mV,最大功率密度达到12.5mW/cm,高于Pd-TiO_(2)/C(50∶50)的12.4mW/cm、Pd-TiO_(2)/C(90∶10)的7.9mW/cm、Pd/C的3.7mW/cm以及TiO_(2)/C的2.25 mW/cm,这表明,TiO_(2)对Pd/C催化剂有很好的促进作用,这种协同效应可以归因为TiO_(2)对Pd的电子效应和双功能机理,同时Pd和TiO_(2)比例为70∶30时,其催化性能达到最优。ATR-FTIR结果表明,反应后所有Pd-TiO_(2)/C电催化剂表面均有甘油醛、羟丙酮酸、甲酸等高附加值产物生成,为直接丙三醇燃料电池电-化学品联产提供一定的依据。

关键词：丙三醇氧化 Pd-TiO_(2)电催化剂原位ATR-FTIR 循环伏安碱性燃料电池

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Obtaining morphometric variables from gullies using two methods of interpolation laser scanner data:the case study of Vassouras,Brazil

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Journal of Mountain Science 2020年第12期17卷 3012-3023页

作者： oliveira Carlos Magno Moreira de FRANCELINO Márcio Rocha MENDONCA Bruno Araujo Furtado de ramos Isabela Queiroz Instituto Federal do Norte de Minas Gerais-Campus Arinos Rodovia MG 202Km 407 s/nZona RuralArinos-MG38680-000Brazil departamento de Solos Universidade Federal de VigosaAv.Peter Henry Rolfss/n-Campus UniversitarioViqosa-MG36570-900Brazil departamento de Silvicultura Universidade Federal Rural do Rio de JaneiroBR 465 Km 07Seropedica-RJ23890-000Brazil Instituto do Meio Ambiente e dos Recursos Hidricos do Distrito Federal SEPN 511Bloco C70750-543BrasiliaDFBrazil

Gully erosion is a worldwide problem of land degradation and water quality,and it is also frequent in ***,anthropic influence is the major driver of gully *** study and monitor gullies it is necessary to use specific instruments and methods to obtain accurate *** objective of this study was to use Terrestrial Laser Scanning(TLS) to create digital elevation model(deM) accurately and define morphometric variables that characterize gullies in a mountainous *** different interpolations were evaluated using the Topogrid and GridSurfaceCreate algorithms to elaborate *** profile for gullies was used to assess modeling *** deM of the Gully 1(G1) from the Topogrid algorithm estimated soil loss of 49%,whereas the GridSurfaceCreate algorithm estimated a soil loss of97%,in a period of 1 *** estimated soil loss for the Gully 2(G2) was 14% from the Topogrid,and 8%from the GridSurfaceCreate *** GridSurfaceCreate algorithm underestimated the volume to area ratio for G2 due to a failure on interpolating a region of low point *** Topogrid algorithm represented better the terrain irregularities,as observed through the topographic profiles traced in three regions of G1 and *** analysis showed that the GridSurfaceCreate algorithm presented lower accuracy in estimating *** underestimation trend of this algorithm was also observed in *** gullies showed considerable soil losses,which may reduce the areas suitable for agricultural activities,and silting up of water *** Topogrid algorithm presented satisfactory results,denoting great potential to produce morphometric data of gullies.

关键词： Erosion process Interpolation algorithms Spatial statistics Digital Elevation Model Topographic profile

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Artisanal ceramic factories using wood combustion:A nanoparticles and human health study

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Geoscience Frontiers 2022年第1期13卷 488-497页

作者： Kátia Martinello James C.Hower Diana Pinto Carlos E.Schnorr Guilherme L.Dotto Marcos Leandro Silva oliveira Claudete G.ramos Instituto Inanís Rua Felizardo FurtadoPorto AlegreBrazil department of Civil and Environmental Universidad de la CostaCUCCalle 58#55–66BarranquillaAtlánticoColombia University of Kentucky Department of Earth&Environmental SciencesLexingtonKY 40506USA University of Kentucky Center for Applied Energy Research2540 Research Park DriveLexingtonKY 40511USA Centro de Estudos em Estresse Oxidativo-departamento de Bioquímica-Instituto de Ciências Básicas da Saúde-Universidade Federal do Rio Grande do Sul(UFRGS) Porto AlegreRSBrazil Chemical Engineering department Federal University of Santa MariaUFSMRoraima Avenue1000Santa MariaRS97105-900Brazil departamento de Ingeniería Civil y Arquitectura Universidad de LimaAvenida Javier Prado Este 4600–Santiago de Surco 1503Peru

The ceramics industry,resulting from developments of modern compounds,is a segment of great influence in worldwide *** ceramic factories based on wood combustion have significant risks for the creation and discharge of atmosphere nanoparticles(NPs)and ultra-fine particles(UFPs).At present,there is insufficient recognition on the influence of engineered-NPs on the atmosphere and *** improvements are indispensable to diminish contact with *** present study demonstrates the main NPs and UFPS present in an area of intense artisanal wood-combustion ceramic *** matter was sampled for morphological,chemical,and geochemical studies by sophisticated electron microbeam microscopy,X-Ray Diffraction,and Raman *** NPs configuration(dentify *** amorphous NPs(>10 nm)were produced around the studied artisanal ceramic *** study presents an indication of the recent information on population and work-related contact to NPs in the artisanal ceramic factories and their influence on health.

关键词： Ceramic manufacturing Nanoparticles Advanced characterization Multiple impacts

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Virulence Factors and Biofilm Formation in Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium Isolates in Brazil

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Advances in Microbiology 2023年第6期13卷 299-314页

作者： Bárbara de Azevedo ramos Marília Manta Manta Sivoneide Maria da Silva Rafael Artur Queiroz Cavalcanti de Sá Natália Lira de Souza Henrique Douglas melo Coutinho Márcia Vanusa da Silva Túlio Diego da Silva Maria Tereza dos Santos Correia Maria Betânia melo de oliveira Universidade Federal de Pernambuco Pernambuco Brazil Universidade Regional do Cariri Ceará Brazil Instituto de Tecnologia de Pernambuco Pernambuco Brazil

In this work, we evaluated biofilm formation of Vancomycin Resistant of E. faecalis and E. faecium (VRE) in different culture media and adhesion substrate, as well as cellular hydrophobicity and presence of virulence genes. For this, 35 isolates were collected from a public hospital in Recife, Pernambuco, Brazil and identified by the Matrix-Assisted Laser desorption Ionization - Time-of-flight - Mass Spectrometry (MALDI-TOF-MS) technique. Biofilm formation was analyzed by the Crystal Violet (CV) method and fluorescence microscopy, cellular hydrophobicity by hydrocarbon interaction and the presence of gelE, esp and asa1 genes by Polymerase Chain Reaction (PCR). 12 isolates were identified as E. faecalis and 23 as E. faecium. Most were obtained in Coronary Units (40.0%) and Intensive Care Unit (31.4%). E. faecium isolates were more resistant to the antibiotics tested than E. faecalis;however, E. faecalis stood out as a biofilm producer. Regarding the presence and gene frequency, it was observed that gelE (54.3%) and esp (54.3%) were the most prevalent, followed by asa1 (22.9%). When comparing the gene frequency, it was observed that gelE and esp were predominant (48.6% for both species), while asa1 was more frequent in E. faecalis (20.0%). The data presented here are worrying, because they reveal the virulence potential of isolates VRE, which contributes to the dissemination and persistence of these pathogens in the hospital environment.

关键词： Biofilm Cellular Hydrophobicity Gram-Positive Hospital Environment Virulence Genes

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Remodeling of the cell wall as a drought-tolerance mechanism of a soybean genotype revealed by global gene expression analysis

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aBIOTECH 2021年第1期2卷 14-31页

作者： Flaviane Silva Coutinho Juliano Mendonça Rodrigues Lucas Leal Lima Rosilene oliveira Mesquita Paola Avelar Carpinetti João Paulo Batista Machado Camilo Elber Vital Pedro Marcus Vidigal Maria Eduarda Soares ramos Mariana Rocha Maximiano Angela Mehta Maria Goreti Almeida oliveira Elizabeth Pacheco Batista Fontes Humberto Josuéde oliveira ramos departamento de Bioquímica e Biologia Molecular Universidade Federal de ViçsaViçsaMinas GeraisBrazil departamento de Fitotecnia Universidade Federal do CearáFortalezaCearáBrazil Núcleo de Análise de Biomoléculas(NuBioMol) Universidade Federal de ViçsaViçsaMinas GeraisBrazil Instituto de Ciéncias Agrárias Universidade Federal de ViçsaFlorestalMinas GeraisBrazil Embrapa Recursos Genéticos e Biotecnologia BrasíliaDFBrazil Universidade Federal de Juiz de Fora Juiz de ForaMinas GeraisBrazil

Drought stress is major abiotic stress that affects soybean ***,it is widely desirable that soybean becomes more tolerant to *** provide insights into regulatory mechanisms of the stress response,we compared the global gene expression profiles from leaves of two soybean genotypes that display different responses to water-deficit(BR 16 and Embrapa 48,drought-sensitive and droughttolerant,respectively).After the RNA-seq analysis,a total of 5335 down-regulated and 3170 up-regulated genes were identified in the *** the other hand,the number of genes differentially expressed was markedly lower in the Embrapa 48,355 up-regulated and 471 down-regulated ***,induction and expression of protein kinases and transcription factors indicated signaling cascades involved in the drought ***,the results suggest that the metabolism of pectin is differently modulated in response to drought stress and may play a role in the soybean defense mechanism against *** occurs via an increase of the cell wall plasticity and crosslink,which contributed to a higher hydraulic conductance(Kf)and relative water content(RWC%).The drought-tolerance mechanism of the Embrapa 48 genotype involves remodeling of the cell wall and increase of the hydraulic conductance to the maintenance of cell turgor and metabolic processes,resulting in the highest leaf RWC,photosynthetic rate(A),transpiration(E)and carboxylation(A/Ci).Thus,we concluded that the cell wall adjustment under drought is important for a more efficient water use which promoted a more active photosynthetic metabolism,maintaining higher plant growth under drought stress.

关键词： Gene expression RNA-seq Molecular physiology Water-use efficiency

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Metabolomics in chronic hepatitis C:decoding fibrosis grading and underlying pathways

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World Journal of Hepatology 2023年第11期15卷 1237-1249页

作者： Adriana Camargo Ferrasi Samara Vitória Granja Lima Aline Faria Galvani Jeany delafiori Flavia Luísa Dias-Audibert Rodrigo ramos Catharino Giovanni Faria Silva Roberta Rodrigues Praxedes Driele Bretones Santos Dayane Trevisan de Macedo Almeida Estela oliveira Lima department of Internal Medicine Sao Paulo State UniversityBotucatu 18618-686Brazil Innovare Biomarkers Laboratory University of CampinasCampinas 13083-877Brazil

BACKGROUND Chronic Hepatitis C(CHC)affects 71 million people globally and leads to liver issues such as fibrosis,cirrhosis,cancer,and death.A better understanding and prognosis of liver involvement are vital to reduce morbidity and *** accurate identification of the fibrosis stage is crucial for making treatment decisions and predicting *** used to grade fibrosis include histological analysis and imaging but have *** markers such as molecular biomarkers can offer valuable insights into *** To identify potential biomarkers that might stratify these lesions and add information about the molecular mechanisms involved in the *** Plasma samples were collected from 46 patients with hepatitis C and classified into fibrosis grades F1(n=13),F2(n=12),F3(n=6),and F4(n=15).To ensure that the identified biomarkers were exclusive to liver lesions(CHC fibrosis),healthy volunteer participants(n=50)were also *** untargeted metabolomic technique was used to analyze the plasma metabolites using mass spectrometry and database *** analyses were performed to identify differential biomarkers among *** Six differential metabolites were identified in each grade of *** six-metabolite profile was able to establish a clustering tendency in patients with the same grade of fibrosis;thus,they showed greater efficiency in discriminating *** This study suggests that some of the observed biomarkers,once validated,have the potential to be applied as prognostic ***,it suggests that liquid biopsy analyses of plasma metabolites are a good source of molecular biomarkers capable of stratifying patients with CHC according to fibrosis grade.

关键词： Chronic Hepatitis C Fibrosis Metabolome Biomarkers Plasma Liquid biopsy

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