检索结果-南通市图书馆

Experimental Study of Rat Beta Islet Cells Cultured under Simulated Microgravity Conditions

Acta Biochimica et Biophysica Sinica 2004年第1期36卷 47-50页

作者： Chun SONG, Xiu-Qing DUAN, Xi LI, Li-Ou HAN, Ping XU, Chun-Fang SONG, and Lian-Hong JIN (key laboratory of Cell Transplantation of Ministry of Health, First Affiliated Hospital of harbin Medical University, harbin 150001, china Department of General Surgery, First Affiliated Hospital of harbin Medical University, harbin 150001, china national key laboratory of harbin veterinary research institute of chinese agriculture academy, harbin 150001, china Tissue Engineering research Center, Histology and Embryology Staff Room, harbin Medical University, harbin 150086, china ) key laboratory of Cell Transplantation of Ministry of Health First Affiliated Hospital of Harbin Medical University Harbin 150001 China Department of General Surgery First Affiliated Hospital of Harbin Medical University Harbin 150001 China national key laboratory of harbin veterinary research institute of chinese agriculture academy harbin 150001 China Tissue Engineering research Center Histology and Embryology Staff Room Harbin Medical University Harbin 150086 China

To observe the effects of simulated microgravity on beta islet cell culture, we have compared the survival rates and the insulin levels of the isolated rat islet cells cultured at micro- and normal gravity conditions. The survival rates of the cells cultured were determined by acridine orange-propidium iodide double-staining on day 3, 7 and 14. The morphology of the cells was observed by electron microscopy. Insulin levels were measured by radio immuno assays. Our results show that the cell number cultured under the microgravity condition is significantly higher than that under the routine condition (P<0.01). Some tubular structure shown by transmission electron microscopy, possibly for the transport of nutrients, were formed intercellularly in the microgravity cultured group on day 7. There were also abundant secretion particles and mitochondria in the cytoplasm of the cells. Scanning electron microscopy showed that there were holes formed between each islet, possibly connecting with the nutrient trans

关键词： simulated microgravity islet cell electron microscopy radioimmunoassay

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

H5N1 avian influenza in china

引用

Science china(Life Sciences) 2009年第5期52卷 419-427页

作者： CHEN HuaLan Animal Influenza laboratory of the Ministry of agriculture and national key laboratory of veterinary Biotechnology, harbin veterinary research institute, chinese academy of Agricultural Sciences, harbin 150001, china Animal Influenza laboratory of the Ministry of agriculture and national key laboratory of veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin China

H5N1 highly pathogenic avian influenza virus was first detected in a goose in Guangdong Province of china in 1996. Multiple genotypes of H5N1 viruses have been identified from apparently healthy waterfowl since 1999. In the years 2004-2008, over 100 outbreaks in domestic poultry occurred in 23 provinces and caused severe economic damage to the poultry industry in china. Beginning from 2004, a culling plus vaccination strategy has been implemented for the control of epidemics. Since then, over 35420000 poultry have been depopulated, and over 55 billion doses of the different vaccines have been used to control the outbreaks. Although it is logistically impossible to vaccinate every single bird in china due to the large poultry population and the complicated rearing styles, there is no doubt that the increased vaccination coverage has resulted in decreased disease epidemic and environmental virus loading. The experience in china suggests that vaccination has played an important role in the protection of poultry from H5N1 virus infection, the reduction of virus load in the environment, and the prevention of H5N1 virus transmission from poultry to humans.

关键词： avian influenza H5N1 control china

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Speckle Noise Suppressing Based on a Generalized Parallel Weighted-Average Multidirectional Morphological Filtering Algorithm

引用

chinese Journal of Lasers 2000年第2期9卷 182-186页

作者： JIANG Lihui WANG Chunhui WANG Qi SHANG Tieliang (national key laboratory of Tunable Laser Technology, harbin institute of Technology, harbin 150001, china ) 哈尔滨工业大学可调谐(气体)激光技术国防科技重点实验室黑龙江哈尔滨

In this paper, a generalized parallel weighted-average multidirectional morphological filtering algorithm is presented to suppresing speckle noise. Simulation shows the algorithm can not only effectively suppress speckle noise but also preserve the geometrical features of images. Through comparison, it is proved that the algorithm is superior to F. Safa algoritm.

关键词： speckle noise morphological filter weighted averages parallel processing

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Study on RF Excited Waveguide CO2 Laser with Two Different Channels

引用

chinese Journal of Lasers 1999年第6期8卷 3-5页

作者： TIAN Zhaoshuo WANG Qi WAN Yusan LIU Fengmei (national key laboratory of Tunable Laser Technology, harbin institute of Technology, harbin 150001, china) 哈尔滨工业大学可调谐（气体）激光技术国防科技重点实验室黑龙江哈尔滨 150001 哈尔滨工业大学可调谐（气体）激光技术国防科技重点实验室黑龙江哈尔滨

In this paper a waveguide CO 2 laser with two unequal long channels excited by radio frequency (RF) is presented. The volume is 2.25×2.25×400 mm 3 for the long channel and 2.25×2.25×200 mm 3 for the short waveguid... 详细信息

关键词： two unequal long channels RF excited waveguide CO 2 laser

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A research on Investment Casting Technology of Ti Alloys

引用

Journal of Materials Science & Technology 2000年第1期16卷 76-78页

作者： Huiguan WAN, Xiumei QI, Jing TIAN, Yuyong CHEN, Weiqiang ZHA and Jun JIA (national key laboratory of Precision Hot Processing of Metals, harbin institute of Technology, harbin 150001, china) national key laboratory of Precision Hot Processing of Metals Harbin Institute of Technology Harbin 150001 China

In this research, the materials and the compositions of the surface slurries were chosen by considering the characteristics of Ti investment casting. The effects of solid-liquid ratios on the properties of the slurry and the effects of baking temperatures on the flexural strength have also been investigated. Flawless shells having smooth inner surface were manufactured with proper technology. Ti and its alloys were melted and poured by water-cooled Cu crucible vacuum induction furnace. The qualities of the investment castings made accordingly were studied and analyzed.

关键词： In Ti A research on Investment Casting Technology of Ti Alloys

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

THERMAL PROCESS OF VACUUM FLUXLESS LASER SOLDERING AND ANALYSIS ON SOLDER SPREADING AND WETTING

引用

chinese Journal of Mechanical Engineering 2000年第2期13卷 127-133页

作者： Wang Chunqing Li Mingyu SunFujiang Feng Wufeng (national key laboratory of welding, harbin institute of Technology) 哈尔滨工业大学现代焊接生产技术国家重点实验室黑龙江哈尔滨

In order to study the mechanism of vacuum fluxless soldering on the conditions of laser heating, the method of measuring temperature by the thermocouple is used to analyze the spreading and wetting process of both fluxless Sn-Pb solder in the vacuum surroundings and flux Sn-Pb solder on Cu pad. Solder spreading and wetting affected by the soldering thermal process is also discussed according to the thermodynamics principle. Results show that vacuum fluxless soldering demands higher temperature, and the fall of the solder surface tension is the important factor achieving fluxless laser soldering.

关键词： Fluxless soldering Soldering thermal process Spreading and wetting

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression of gelatinases and tissue inhibitors of metallo-proteinases in the rhesus monkey(Macaca mulatta) corpus luteum

引用

chinese Science Bulletin 2001年第15期46卷 1292-1296页

作者： LI Qinglei, WANG Hongmei, SHAO Longjiang, YU Xiaoguang, Nl Jiang & ZHU Cheng1. State key laboratory of Reproductive Biology, institute of Zoology, chinese academy of Sciences, Beijing 100080, china 2. laboratory of Reproductive Endocrinology, harbin Medical University, harbin 150001, china State key laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China laboratory of Reproductive Endocrinology Harbin Medical University Harbin China

Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are believed to play important roles in the formation and regression of corpus luteum (CL). This study is to investigate the expression of gelatinases (MMP-2, -9) and TIMPs in the rhesus monkey CL in both early and late luteal phases and during the early stages of pregnancy. Ovaries were collected from regularly cycling rhesus monkey at D5 and D15 following ovulation and at D12, D18 and D26 of pregnancy. In situ hybridization revealed that in the CL MMP-2 niRNA was expressed during both formation and regression, while MMP-9 mRNA was mainly localized in the late luteal phase. Reduction of MMP-2, -9 transcripts in the CL was observed during pregnancy. MMP-2 mRNA in the CL reduced to an undetectable level at D26 of pregnancy. TIMP-1 mRNA was highly expressed in the CL in both early and late luteal phases and persisted throughout the early stages of pregnancy. Strong signal for TIMP-2 mRNA was also detected in both luteal phases, and the

关键词： matrix metalloproteinases tissue inhibitors of metallo-proteinases gelatinases macaca mulatta corpus luteum.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

research ON THE MINIMUM ZONE CYLINDRICITY EVALUATION BASED ON GENETIC ALGORITHMS

引用

chinese Journal of Mechanical Engineering 2003年第2期16卷 167-170页

作者： Cui ChangcaiChe RenshengYe DongHuang QingchengDepartment of Automatic Measurement and Control,harbin institute of Technology, harbin 150001, china Department of Automatic Measurement and Control Harbin Institute of Technology Harbin 150001

A genetic algorithm (GA)-based method is proposed to solve the nonlinearoptimization problem of minimum zone cylindricity evaluation. First, the background of the problemis introduced. Then the mathematical model and the fitness function are derived from themathematical definition of dimensioning and tolerancing principles. Thirdly with the least squaressolution as the initial values, the whole implementation process of the algorithm is realized inwhich some key techniques, for example, variables representing, population initializing and suchbasic operations as selection, crossover and mutation, are discussed in detail. Finally, examplesare quoted to verify the proposed algorithm. The computation results indicate that the GA-basedoptimization method performs well on cylindricity evaluation. The outstanding advantages concludehigh accuracy, high efficiency and capabilities of solving complicated nonlinear and large spaceproblems.

关键词： genetic algorithm (GA) cylindricity form error minimum zone

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tensile and compression properties of Zr-based bulk amorphous alloy at different temperatures

引用

Science china(Technological Sciences) 2005年第5期48卷 489-495页

作者： WANG Xu1,3, LOU Decheng1, GAO Zhanjun4, LIU Lei1, LIANG Hongmei1, WANG Qingfeng1, LI Qiang1,3 & MA Mingzhen1,2 1. key laboratory of Metastable Materials Science & Technology of Hebei, Yanshan University, Qinhuangdao 066004, china 2. national key laboratory for Precision Hot Processing of Metals, harbin institute of Technology, harbin 150001, china 3. College of Mechanical Engineering, Yanshan University, Qinhuangdao 066004, china 4. chinese academy of Engineering, Beijing 100038, china key laboratory of Metastable Materials Science & Technology of Hebei Yanshan University Qinhuangdao China College of Mechanical Engineering Yanshan University Qinhuangdao China chinese academy of Engineering Beijing China national key laboratory for Precision Hot Processing of Metals Harbin Institute of Technology Harbin China

Mechanical properties of the Zr41Ti14Cu12.5Ni10Be22.5 bulk amorphous alloy at different temperatures were investigated. The compression test was carried out on a Gleebe-3200 machine at 345 and 375°C, respectively, in the supercooled liquid region. It is shown that decreasing the compressive rate and increasing temperature have a similar influence trend on the compressive behavior of the bulk amorphous alloy. Room and low temperature tensile strengths were tested on the Instron materials testing system. At low temperature, the tensile strength decreased with decreasing of the testing temperature. Hardness measurement indicated that below the glass transition temperature, the hardness decreased with increasing of the annealing temperature and duration time. It, however, increased when the annealing treatment was performed above the glass transition temperature.

关键词： Zr41Ti14Cu12.5Ni10Be22.5 bulk amorphous alloy, mechanical properties, compressive deformation.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A simple and rapid immunochromatographic strip test for monitoring antibodies to H5 subtype Avian Influenza Virus

A simple and rapid immunochromatographic strip test for moni...

引用

第六届全国免疫学学术大会

作者： Shangjin Cui Guangzhi Tong national key laboratory of veterinary Biotechnology,harbin veterinary research institute,chinese academy of Agricultural Sciences,harbin 150001,china

A colloid gold strip(CGS)test for detecting antibodies to H5 subtype Avian Influenza Virus (AIV)was *** test is based on membrane chromatography and uses colloidal gold conjugated with inactivated AIV H5N1 as the *** ...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：