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Differentiation of hmSC in micromass Culture

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生物医学工程学杂志 2005年第S1期22卷 153-154页

作者： Jing-WEI YANG natalia De ISIA Céline HUSELSTEIN Luc mARCHAL marie-Nathalie SARDA-KOLOPP Jing-Ping OUYANG Jean-Fran·ois STOL TZ Assia ELJAAFARI Research Group of Cell and Tissue Engineering LEMTA-CNRS UMR 7563Facultéde Médecine de NancyUHP and CHU NancyFrance Department of Pathophysiology Medical College of Wuhan UniversityWuhan 430071China Immunology Laboratory Faculty of MedicineUHP Nancy 1Vandoeuvre-lès-NancyFrance

1 Introduction mesenchymal stem cells(mSCs)are multipotential stem cells which can be expanded in culture while still maintaining their undifferentiated *** have the potential to differentiate into distinct mesenchymal tissue cells,including ***,they are an attrac2 tive cell source for cartilage ti ssue *** vitro high density micromass culture has been widely used for chondrogenesis *** objective of our study was to analyze viability and differentiation of hmSC in micro2 mass cultures.

关键词： stem cells tissue engineering high density

Esterase Activities and Biochemical Properties of Lactic Acid Bacteria Isolated from Goat＇s milk Cheese in Argentina

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Journal of Agricultural Science and Technology(B) 2014年第9期4卷 752-760页

作者： natalia Veronica Taboada maria Soledad Lopez Alzogaray maria Claudia Abeijon mukdsi Roxana Beatriz medina Departamento de Ciencias de los Alimentos Faeultad de Agronomia y Agroindustrias Universidad Nacional de Santiago delEstero Av. Belgrano 1912 (S) Santiago del Estero CP 4200 Argentina Centro de Refereneia Para Lactobacilos (CERELA-CONICET) Chacabuco 145 Tucum(m CP 4000 Argentina Faeultad de Ciencias de la Salud Universidad del Norte Santo de Aquino 9 de Julio 165 Tueumdn CP 4000 Argentina Facultad de Agronomia y Zootecnia Universidad Nacional de Tucumdn Av. Roca 1900 Tucumdn CP 4000 Argentina

Twenty-two lactic acid bacteria （LAB） strains isolated from Argentinean goat dairy products were evaluated for its biochemical properties and esterase activities relevant to flavor development. Streptococcus thermophilus （UNSE314）, Lactobacillus （L.） delbrueckii subsp, bulgaricus （UNSE309）, L. rhamnosus （UNSE308）, L. plantarum （UNSE287, UNSE316, UNSE317） and Pediococcus pentosaceus （UNSE315） strains presented high acidifying activity. All strains tested metabolized citrate and produced diacetyl-acetoin in goat milk. Based on these results, 10 strains with the best performance in diverse technological properties were selected to determine esterolytic activity. In all evaluated strains, esterase specific activity （ESA） was detected on ct-naphthyl （ct-NA） acetate and 13-naphthyl （[3-NA） acetate, propionate, eaprylate and ct-NA butyrate. No activity was detected on [3-NA laurate. The highest values were detected when using a-NA instead of fI-NA derivatives as substrate. In Pediocoecus strains, wide variability in ESA were observed, which were species- and strain-specific. These results allow us to select strains with biochemical properties and esterase activities to design starter and adjunct cultures that contribute to flavor development during cheese ripening, thus preserving the typical organoleptic characteristics of Argentinean goat cheeses.

关键词： Esterase activity Pediococcus autochthonous starter cultures biochemical properties goat's milk cheese.

Interaction of Coal Humic Acids with Fungal Laccase

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Advances in microbiology 2013年第2期3卷 145-153页

作者： natalia A. Kulikova Valentina N. Davidchik Eugenia A. Tsvetkova Olga V. Koroleva Department of Soil Science M.V. Lomonosov Moscow State University Moscow Russia

Humic acids (HA) are one of the main environmental factors controlling the fate and behavior of the compounds released into the environment. In particular, they are universally considered of great importance in determining soil extracellular enzyme activity and stability via association with essential soil enzymes. The objective of this study was to investigate the interaction of coal HA with an extracellular multicopper oxidase laccase (EC 1.***.3.2) that catalyze the oxidation of a wide range of reducing substances in the environment. Using size-exclusion chromatography analysis and monitoring laccase activity, the formation of a stable and an enzymatically active complex between HA and laccase was shown. Basing the data obtained by isoelectric focusing of HA-laccase complex, non-covalent character of laccase association with HA was considered and binding of laccase to HA by weak dispersive forces such as van der Waals, hydrophobic, π-π, CH-π and others was hypothesized.

关键词： Humic Acids Laccase Activity Size-Exclusion Chromatography Complexes Isoelectric Focusing

Interaction of Hemoglobin with Binuclearcationic Tetranitrosyl Iron Complex with Penicillamine. Cations Binding Sites

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Advances in Biological Chemistry 2015年第3期5卷 169-178页

作者： Lidia Syrtsova natalia Sanina Boris Psikha Ildar Tukhvatullin Natal’ja Shkondina Olesia Pokidova Alexander Kotelnikov Institute of Problems of Chemical Physics of the Russian Academy of Sciences Moscow Region Russian Federation

In this paper, the kinetics of the interaction of the nitrosyl iron complex with the ligands penicillamine [Fe2(SC5H11NО2)2(NO)4]SO4·5H2O (I) with deoxyhemoglobin (Hb) was studied. The kinetic modeling method defined the number of binding (I) molecules and equilibrium constant of the coupling reaction of (Biomedicine, Iron-Sulfur Cluster, Ligand Binding, Heme, Nitric Oxide ) with Hb (Ks). At equimolar concentrations of (I) and Hb (2 × 10−5 m), the Hb molecule binds only one (I) with Ks equal to 4.3 × 107 m−1. When increasing the (Biomedicine, Iron-Sulfur Cluster, Ligand Binding, Heme, Nitric Oxide ) concentration, the number of binding sites of Hb increases and Ks decreases. These results are analyzed in accordance with the data on the existence of cations binding sites in Hb.

关键词： Biomedicine Iron-Sulfur Cluster Ligand Binding Heme Nitric Oxide

The Cyclic Feeding Regime Induces Decaying Age-Dependent Oxidative Stress and Regulates the Cell Chain of the Immunity

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Advances in Aging Research 2016年第6期5卷 151-165页

作者： Anatoly I. Bozhkov Yuriy V. Nikitchenko Katerina N. Lebed’ Olena S. Linkevych natalia I. Kurguzova Olena m. Klimova mohammad A. Y. Al Begai Ali m. m. Al-Bahadly mohammad m. A. Alsardia Research Institute of Biology V.N. Karazin Kharkov National University Kharkov Ukraine

We developed an experimental model of a cyclic feeding regime (CFR) that increased a lifespan in rats. The manifestations of oxidative stress and their interrelation with parameters of cell immunity were assessed in rats at CFR. It is shown that changes of body mass, liver mass and indexes of pro/antioxidant system after periods of starving—ad libitum nutrition at CFR diet in old animals were less pronounced than in young animals. The body mass loss of 30% in 14 days was accompanied by oxidative stress. Indexes of phagocytosis did not change, but activity of oxidase system of neutrophil was increased in 2 times. The response of metabolic and physiological systems on repeat starving—ad libitum nutrition cycles differs from the response to the initial cycle of CFR. This is interpreted as a change of adaptation strategy and the effect of metabolic memory, which influences the choice of organism strategy of adaptation for subsequent starving. The dynamics of change of the studied indexes in response to CFR was age-dependent. It was supposed that different answer to CFR in young and old animals is determined by the different amount of carbon and fat depots in young and old animals.

关键词： Aging Oxidative Stress Cyclic Feeding Regime Primary Immunity Pro-Oxidant System

TP53 codon 72 Arg/Arg polymorphism is associated with a higher risk for inflammatory bowel disease development

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World Journal of Gastroenterology 2015年第36期21卷 10358-10366页

作者： natalia Volodko mohamed Salla Bertus Eksteen Richard N Fedorak Hien Q Huynh Shairaz Baksh Department of Pediatrics Faculty of Medicine and DentistryUniversity of Alberta Department of Biochemistry Faculty of Medicine and DentistryUniversity of Alberta Department of medicine Division of Gastro-enterologyUniversity of Calgary Department of medicine Faculty of Medicine and DentistryUniversity of Alberta Department of Oncology Faculty of Medicine and DentistryUniversity of Alberta

AIm: To investigate the association between tumor protein 53(TP53) codon 72 polymorphisms and the risk for inflammatory bowel disease(IBD) ***: Numerous genetic and epigenetic drivers have been identified for IBD including the TP53 gene. Pathogenic mutations in TP53 gene have only been reported in 50% of colorectal cancer(CRC) patients. A single nucleotide polymorphism(SNP) in the TP53 gene resulting in the presence of either arginine(Arg)or proline(Pro) or both at codon 72 was shown to alter TP53 tumor-suppressor properties. This SNP has been investigated as a risk factor for numerous cancers,including CRC. In this study we analyzed TP53 codon 72 polymorphism distribution in 461 IBD,181 primary sclerosing cholangitis patients and 62 healthy controls. Genotyping of TP53 was performed by sequencing and restriction fragment length polymorphism analysis of genomic DNA extracted from peripheral blood. RESULTS: The most frequent TP53 genotype in IBD patients was Arg/Arg occurring in 54%-64% of cases(and in only 32% of controls). Arg/Pro was the most prevalent genotype in controls(53%) and less common in patients(31%-40%). Pro/Pro frequency was not significantly different between controls and IBD patients. CONCLUSION: The data suggests that the TP53 codon 72 Arg/Arg genotype is associated with increased risk for IBD development.

关键词： Inflammatory bowel disease Colorectal cancer 72 co

Statistical Characteristics of Scattered Radiation in medium With Spatial-temporal Fluctuations of Electron Density and External magnetic Field

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Journal of Electromagnetic Analysis and Applications 2012年第6期4卷 243-251页

作者： George V. Jandieri natalia N. Zhukova Irma G. Takidze Ivane V. Jandieri Department of Physics Georgian Technical University Georgian Technical University Tbilisi Georgia Department of Physics Georgian Technical University Tbilisi Georgia Dynamics of Geophysical fields and Computing Geophysics TSU M. Nodia Institute of Geophysics Tbilisi Georgia.

Influence of temporal fluctuations of both electron density and external magnetic field fluctuations on scattered ordinary and extraordinary waves in magnetized plasma is investigated using the ray-(optics) method. Transport equation for frequency fluctuations of scattered radiation has been derived. Broadening of the spatial power spectrum and amplification of the intensity of frequency fluctuation taking into account geometry of the task and the features of turbulent magnetized plasma is analyzed for the anisotropic Gaussian correlation function using the remote sensing data. It is shown that spatial-temporal fluctuations of electron density and external magnetic field, anisotropy and angle of inclination of prolate irregularities relative to the external magnetic field may lead to the exponential amplification of the intensity of frequency fluctuations of scattered electromagnetic waves in the collisional magnetized plasma.

关键词： Fluctuations Correlation function Scattering magnetized Plasma Phase portrait Extraordinary Wave

基于SPR分析的金纳米粒子-适配体传感器检测梯恩梯

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分析试验室 2018年第12期37卷 1421-1425页

作者：谭俊鹏 natalia Pawlowska 徐升晖蔡能斌杨瑞琴郝红霞证据科学教育部重点实验室(中国政法大学) 2011年计划司法文明协同创新中心北京100088 中国人民公安大学北京100038 张家口市公安局物证鉴定所张家口075000 上海市现场物证重点实验室上海200083

构建了基于表面等离子体共振(SPR)分析的金纳米粒子(Au NPs)-TNT适配体传感器。该方法将TNT适配体运用在SPR检测方法中,并与Au NPs合成复合物以提高灵敏度。传感器芯片使用聚乙二醇结合2,4,6-三硝基苯基-甘氨酸进行修饰,将Au NPs-TNT适... 详细信息

构建了基于表面等离子体共振(SPR)分析的金纳米粒子(Au NPs)-TNT适配体传感器。该方法将TNT适配体运用在SPR检测方法中,并与Au NPs合成复合物以提高灵敏度。传感器芯片使用聚乙二醇结合2,4,6-三硝基苯基-甘氨酸进行修饰,将Au NPs-TNT适配体复合物作为配体,通过间接竞争法检测TNT。实验对溶液pH、进样时间、进样流速以及孵育时间进行优化。最优条件下(溶液pH 9.62、进样时间1500S、进样流速500μL/min、孵育时间30min),检测线性范围为0.5~20ng/mL,检出限58pg/mL(S/N=3)。该传感器可用于检测实际水样中TNT,在公共安全及环境监测领域具有良好的应用前景。

关键词： 2,4,6-三硝基甲苯表面等离子体共振适配体金纳米粒子

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雌激素减少与代谢综合征关系的实验研究

山东大学学报（医学版） 2006年第5期44卷 492-495,500页

作者：王敏王磊任续功单祥鸣吴丽娜吕春梅袁广梅 m.Ram Sairam natalia Danilovich 德州市人民医院内二科山东德州253014 德州市立医院保健科山东德州253000 加拿大蒙特利尔临床研究所分子生殖实验室

目的：探讨雌激素减少与代谢综合征的相关性。方法：在基因库中搜索已知的对脂肪组织有影响的10个基因，筛选其启动子中可能存在的雌激素调控因子的基因。以不同月龄、不同基因型的卵泡刺激素受体敲除（FORKO）雌鼠为动物模型，测量其... 详细信息

目的：探讨雌激素减少与代谢综合征的相关性。方法：在基因库中搜索已知的对脂肪组织有影响的10个基因，筛选其启动子中可能存在的雌激素调控因子的基因。以不同月龄、不同基因型的卵泡刺激素受体敲除（FORKO）雌鼠为动物模型，测量其腹内白色脂肪组织（WAT）重量、体重，计算腹内WAT与体重的比率；口服葡萄糖（2g／kg），测快速血糖（0，30，60，120min）行葡萄糖耐量实验；用组织学方法观察脂肪细胞、肝脏细胞的形态；用放射免疫法测定血清瘦素。结杲：①在7个肥胖相关基因中找到了可能的雌激素调控位点；②FORKO雌鼠于3月龄时出现肥胖，腹内WAT的重量明显高于野生型（P〈0.05）；③FORKO雌鼠随月龄的增长葡萄糖耐量逐渐减低（P〈0．05）；④FORKO雌鼠腹内WAT细胞在3月龄时体积较小，表现为增生（P〈0．05），而在8个月时表现为肥大（P〈0．05）；⑤FORKO雌鼠于8月龄时WAT细胞、棕色脂肪（BAT）细胞及肝细胞内均有大量脂酯颗粒堆积；⑥月龄3～5个月的FORKO雌鼠空腹的血清瘦素水平均明显高于野生雌鼠（P〈0．05）。结论：FORKO雌鼠因雌激素减少而表现出腹型肥胖、高血糖、高瘦素和胰岛素抵抗等代谢紊乱，提示雌激素减少可导致代谢综合征。

关键词：雌激素代谢综合征小鼠,基因敲除