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Clustering of Pathogen-Response Genes in the Genome of Arabidopsis thaliana

Journal of Integrative Plant Biology 2011年第10期53卷 824-834页

作者： Olga A.Postnikova natalia Y.minakova Alexander m.Boutanaev lev G.Nemchinov United States Department of Agriculture Agricultural Research ServicePlant Sciences InstituteMolecular Plant Pathology Laboratory Institute of Basic Biological Problems Russian Academy of Sciences

Previously, we used heterologous expressed sequence tag （EST） mapping to generate a profile of 4 935 pathogen-response genes of Arabidopsis thaliana. In this work, we performed a computer analysis of this profile, revealing 1594 non-homologous clustered genes distributed among all A. thafiana chromosomes, whose co-regulation may be related to host responses to pathogens. To supplement computer data, we arbitrarily selected two clusters and analyzed their expression levels in A. thaliana ecotypes Col-0 and C24 during infection with the yellow strain of Cucumber mosaic virus CmV（Y）. Ecotype Col-0 is susceptible to CmV（Y）, whereas C24 contains the dominant resistance gene RCY1. Upon infection with CmV（Y）, all clustered genes were significantly activated in the resistant ecotype C24. In addition, we demonstrated that posttranslational histone modifications associated with trimethylation of histone H3 lysine 27 are most likely involved in regulation of several cluster genes described in this study. Overall, our experiments indicated that pathogen-response genes in the genome of A. thaliana may be clustered and co-regulated.

关键词： CmV H3K27me3 heterologous EST mapping clustering of defense-related genes.

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Practical obstacles and their mitigation strategies in compressional optical coherence elastography of biological tissues

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Journal of Innovative Optical Health Sciences 2017年第6期10卷 51-63页

作者： Vladimir Y.Zaitsev Alexandr L.matveyev lev A.matveev Ekaterina V.Gubarkova Alexandr A.Sovetsky marina A.Sirotkina Grigory V.Gelikonov Elena V.Zagaynova natalia D.Gladkova Alex Vitkin Institute of Applied Physics Russian Academy of Sciences Ulyanova Street 46 Nizhny Novgorod 603950 Russia Nizhny Novgorod State medical Academy 10/1 minina Square Nizhny Novgorod 603005 Russia University of Toronto and University Health Network 610 University Avenue Toronto ON M5G 2M9Canada

In this paper,we point out some practical obstacles arising in realization of compressional optical coherence elastography(OCE)that have not attracted sufficient attention ***-cally,we discuss(i)complications in quantification of the Young modulus of tissues related to partial adhesion between the OCE probe and soft intervening reference layer sensor,(i)distorting influence of tisue suface curvature/corrugation on the subsurface strain distri-bution mapping,(ii)ways of signal-to-noise mtio(SNR)enhancement in OCE strain mapping when periodic averaging is not realized,and(iv)potentially significant infuence of tissue elastic nonlinearity on quantification of its *** pnactical approaches to mitigate the effects of these complications are also described.

关键词： Optical coherence elastography optical coherence tomography phase-resolved methods strain mapping stiffness characterization

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