Clustering of Pathogen-Response Genes in the Genome of Arabidopsis thaliana

作     者：Olga A.Postnikova Natalia Y.Minakova Alexander M.Boutanaev Lev G.Nemchinov 

作者机构：United States Department of AgricultureAgricultural Research ServicePlant Sciences InstituteMolecular Plant Pathology Laboratory Institute of Basic Biological ProblemsRussian Academy of Sciences 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2011年第53卷第10期

页      面：824-834页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 090102[农学-作物遗传育种] 

基　　金：supported by the United States Department of Agriculture Agricultural Research Service 

主　　题：CMV H3K27me3 heterologous EST mapping clustering of defense-related genes. 

摘      要：Previously, we used heterologous expressed sequence tag （EST） mapping to generate a profile of 4 935 pathogen-response genes of Arabidopsis thaliana. In this work, we performed a computer analysis of this profile, revealing 1594 non-homologous clustered genes distributed among all A. thafiana chromosomes, whose co-regulation may be related to host responses to pathogens. To supplement computer data, we arbitrarily selected two clusters and analyzed their expression levels in A. thaliana ecotypes Col-0 and C24 during infection with the yellow strain of Cucumber mosaic virus CMV（Y）. Ecotype Col-0 is susceptible to CMV（Y）, whereas C24 contains the dominant resistance gene RCY1. Upon infection with CMV（Y）, all clustered genes were significantly activated in the resistant ecotype C24. In addition, we demonstrated that posttranslational histone modifications associated with trimethylation of histone H3 lysine 27 are most likely involved in regulation of several cluster genes described in this study. Overall, our experiments indicated that pathogen-response genes in the genome of A. thaliana may be clustered and co-regulated.