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Neural Regeneration Research 2018年第3期13卷 536-540页

作者： Atsushi Takazawa naosuke Kamei Nobuo Adachi Mitsuo Ochi Department of Orthopaedic Surgery Graduate School of Biomedical & Health Sciences Hiroshima University Medical Center for Translational & Clinical Research Hiroshima University Hospital

Old astrocyte specifically induced substance （OASIS） is an endoplasmic reticulum （ER） stress transducer specifically expressed in astrocytes and osteoblasts. OASIS regulates the differentiation of neural precursor cells into astrocytes in the central nervous system. This study aimed to elucidate the involvement of ER stress responses stimulated via OASIS in astrogliosis following spinal cord injury. In a mouse model of spinal cord contusion injury, OASIS mRNA and protein expression were evaluated at days 7 and 14. A significant increase in OASIS mRNA on day 7 and an increase in protein on days 7 and 14 was observed in injured spinal cords. Immunostaining on day 7 revealed co-localization of OASIS and astrocytes in the periphery of the injury site. Furthermore, anti-OASIS small interfering RNA （siRNA） was injected at the injury sites on day 5 to elucidate the function of OASIS. Treatment with anti-OASIS siRNA caused a significant decrease in OASIS mRNA on day 7 and protein on days 7 and 14, and was associated with the inhibition of astrogliosis and hindlimb motor function recovery. Results of our study show that OASIS expression synchronizes with astrogliosis and is functionally associated with astrogliosis after spinal cord injury.

关键词： unfolded protein response cAMP-response element binding protein/activating transcription factor protein family C57BL/6 contusion injury reactive astrocyte functional recovery real-time polymerase chain reaction western blot immunohistochemistry glial fibrillary acidic protein

Isolation of a choline monooxygenase cDNA clone from Amaranthus tricolor and its expressions under stress conditions

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Cell Research 2001年第3期11卷 187-193页

作者： MENG YU LING, YU MEI WANG, DA BING ZHANG, naosuke NII Faculty of Agriculturs, Meijo University, Tempaku-ku, Nagoya, Aichi. 468-8502 Japan Yingdong College of Biotechnology, Shaoguan University, Datang Road, Shaoguan 512005, China Agro-Biotech Center of Sh Faculty of Agriculture Meijo University Tempaku-ku Nagoya Japan Yingdong College of Biotechnology Shaoguan University Shaoguan China Agro-Biotech Center of Shanghai Academy of Agricultural Sciences Shanghai Key Laboratory of genetic breeding Shanghai China

Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine be- taine[1]. Two enzymes are involved in the pathway choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). A full length CMO cDNA (1,643bp) was cloned from Amaranthus tricolor. The open reading frame encoded a 442-amino acid polypeptide, which showed 69% identity with CMOs in Spina- cia oleracea L. and Beta vulgaris L. DNA gel blot analysis indicated the presence of one copy of CMO gene in the A. tricolor genome. The expressions of CMO and BADH proteins in *** leaves significantly increased under salinization, drought and heat stress (42℃), as determined by immunoblot analysis, but did not respond to cold stress (4℃), or exogenous ABA application. The increase of GB content in leaves was parallel to CMO and BADH contents.

关键词： Amaranthus tricolor betaine aldehyde dehydrogenase(BADH) choline monooxygenase (CMO) glycine betaine (GB) stress.

Quantitative Microbiological Evaluation of <i>Salmonella</i>Typhimurium Shed in Diarrhea, Loose, and Normal Stools of Infected Pigs

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Open Journal of Veterinary Medicine 2014年第4期4卷 58-66页

作者： Tsuyoshi Tanaka Yasuo Imai naosuke Kumagae Takashi Sasaki Narutoshi Ochiai Katsuyoshi Uruno Haruki Kitazawa Tadao Saito Shizuo Sato Food and Feed Immunology Group Laboratory of Animal Products Chemistry Graduate School of Agricultural Science Tohoku University Aobaku Sendai Miyagi Japan JA Zen-Noh (National Federation of Agricultural Co-operative Associations) Institute of Animal Health 7 Ohja-machi Sakura Chiba Japan

Control of within-herd transmission of Salmonella is important for reducing the prevalence of this organism on pig farms and for preventing Salmonella-contamination of pork. At the farm level, understanding the within-herd transmission of Salmonella can lead to more effective control. Salmonella infection is dependent on the inoculation dose;hence, quantitative evaluation of Salmonella shed in feces would provide useful information for developing effective measures. In this study, to reproduce and evaluate the number of Salmonella shed in diarrhea, loose stools, and normal feces, weaned pigs were inoculated with 3.2 × 109, 3.2 × 107, and 3.2 × 105 cfu of Salmonella Typhimurium, respectively. The number of S. Typhimurium shed in the feces peaked within 1 week post-inoculation in every group and the most amount of diarrhea and loose stools were observed within 2 weeks post-inoculation. Diarrhea occurred 10 times (six pigs), and loose stools were observed 25 times (11 pigs). The average concentration of S. Typhimurium shed in diarrhea, loose stools, and normal feces was 1.0 × 108, 1.6 × 104, and 7.1 × 101 cfu/g feces, respectively. These data suggest that diarrhea and loose stools are significant sources of within-herd transmission of Salmonella. Moreover, as some of the normal feces contained >1.0 × 106 cfu/g of S. Typhimurium, even normal feces could be a source of within-herd transmission of Salmonella. At Salmonella-positive farms, reduction of the amount of Salmonella shed even in normal feces would lead to better control of within-herd transmission of Salmonella. These data can contribute to the control of within-herd transmission of Salmonella, particularly during the weaning period.

关键词： Fecal Shedding Quantitative Evaluation Salmonella Typhimurium Swine Transmission

Evaluation of the Immunoregulatory Capacities of Feed Microbial Materials in Porcine Intestinal Immune and Epithelial Cells

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Open Journal of Veterinary Medicine 2014年第3期4卷 15-28页

作者： naosuke Kumagae Julio Villena Yohsuke Tomosada Hisakazu Kobayashi Paulraj Kanmani Hisashi Aso Takashi Sasaki Motohiko Yoshida Hiroshi Tanabe Isao Shibata Tadao Saito Haruki Kitazawa Cell Biology Laboratory Graduate School of Agricultural Science Tohoku University Sendai Japan Food and Feed Immunology Group Tohoku University Sendai Japan Food and Feed Immunology Group Tohoku University Sendai Japan Japan Society for the Promotion of Science (JSPS) Tokyo Japan Food and Feed Immunology Group Tohoku University Sendai Japan Laboratory of Immunobiotechnology Reference Centre for Lactobacilli (CERELA-CONICET) Tucuman Argentina Food and Feed Immunology Group Tohoku University Sendai Japan Scientific Feed Laboratory Co. Ltd. Tokyo Japan Scientific Feed Laboratory Co. Ltd. Tokyo Japan

The establishment of drug-free feeding systems has been required for secure and healthy livestock production. Although functional feed materials containing microorganisms as alternatives to enhance intestinal immunity are expected to be beneficial for reducing diarrhoea caused by pathogens in weaned piglets, the effects of such materials on porcine intestinal cells have not been investigated in detail. Therefore, this work evaluated the immunoregulatory functions of microbial feed materials in porcine intestinal immune and epithelial cells. Porcine immune cells isolated from Peyer’s patches and mesenteric lymph nodes were stimulated with six different feed materials containing microorganisms, and evaluated for lymphocyte mitogenicity and cytokine inductions. In addition, porcine intestinal epithelial cells were stimulated with the materials before treatment with heat-killed enterotoxigenic Escherichia coli (ETEC), and analyzed for the proinflammatory cytokine expressions. The material containing Bifidobacterium thermophilum significantly augmented lymphocytes’ mitogenicity and also induced a high expression of IL-2, IL-6 and IFN-γ in immune cells, and inhibited ETEC-induced overexpression of IL-6 and IL-8 via regulation of Toll-like receptor signaling. These results suggest that this feed material stimulates intestinal epithelial and immune cells to exert immunoregulation, suggesting that this feed is expected to contribute to promoting the health of piglets without using antimicrobial feed materials.

关键词： Feed Microbial Material Immunoregulatory Effect Porcine Intestinal Epitheliocytes Porcine Immune Cells

The Protocadherin 17 Gene Affects Cognition, Personality, Amygdala Structure and Function, Synapse Development and Risk of Major Mood Disorders

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The Protocadherin 17 Gene Affects Cognition, Personality, Am...

NaCl胁迫对胡萝卜种子萌发、幼苗生长及叶细胞结构的影响

中国神经科学学会第十二届全国学术会议

作者： Hong Chang naosuke Hoshina Yina Ma Hengyi Cao Andreas Meyer-Lindenberg Heike Tost Xiao Xiao Tadashi Yamamoto Ming Li Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of Zoology Cell Signal Unit Okinawa Institute of Science and Technology Graduate University State Key Laboratory of Cognitive Neuroscience and Learning IDG/McGovern Institute for Brain Research Beijing Normal University Department of Psychiatry and Psychotherapy Central Institute of Mental Health Medical Faculty Mannheim University of Heidelberg

Objective: Major mood disorders, which primarily include bipolar disorder and major depressive disorder, are the leading cause of disability worldwide and pose a major challenge in identifying robust risk genes. Methods: An integrative analysis involving genetics, m RNA expression, cognitive performance, neuroimaging and neurobiology was performed. Results: We present data from independent large-scale clinical datasets（including 29,557 cases and 32,056 controls） revealing brain expressed protocadherin 17（PCDH17） as a susceptibility gene for major mood disorders. Single nucleotide polymorphisms（SNPs） spanning the PCDH17 region are significantly associated with major mood disorders;subjects carrying the risk allele showed impaired cognitive abilities, increased vulnerable personality features, decreased amygdala volume and altered amygdala function as compared with non-carriers. The risk allele predicted higher transcriptional levels of PCDH17 m RNA in postmortem brain samples, which is consistent with increased gene expression in patients with bipolar disorder compared with healthy subjects. Further, overexpression of PCDH17 in primary cortical neurons revealed significantly decreased spine density and abnormal dendritic morphology compared with control groups, which again is consistent with the clinical observations of reduced numbers of dendritic spines in the brains of patients with major mood disorders. Conclusions: Given that synaptic spines are dynamic structures which regulate neuronal plasticity and play crucial roles in myriad brain functions, this study reveals a potential underlying biological mechanism of a novel risk gene for major mood disorders involved in synaptic function and related intermediate phenotypes.

关键词： PCDH17 mood disorders amygdala expression dendritic spines

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华北农学报 2005年第4期20卷 21-24页

作者：崔世茂陈源闽新居直祐赵彦内蒙古农业大学农学院内蒙古呼和浩特010018 内蒙古农科院蔬菜研究所内蒙古呼和浩特010031 日本名城大学农学部

NaCl胁迫明显抑制胡萝卜种子萌发,当NaCl浓度为50 mmol/L时,种子发芽率下降1/2,当NaCl浓度达到150 mmol/L时,种子发芽率下降82.9%;无论NaCl浓度高低,都对胡萝卜幼苗生长产生抑制作用,使幼苗生长量、干物质积累及含水量显著下降。NaCl浓... 详细信息

NaCl胁迫明显抑制胡萝卜种子萌发,当NaCl浓度为50 mmol/L时,种子发芽率下降1/2,当NaCl浓度达到150 mmol/L时,种子发芽率下降82.9%;无论NaCl浓度高低,都对胡萝卜幼苗生长产生抑制作用,使幼苗生长量、干物质积累及含水量显著下降。NaCl浓度对叶细胞结构也有很大影响,随着NaCl浓度的升高,细胞核缩小、变形或降解。

关键词：盐胁迫胡萝卜发芽率幼苗生长细胞核

NaCI胁迫对不同地域胡萝卜种子萌发特性的影响

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内蒙古农业大学学报（自然科学版） 2010年第3期31卷 91-94页

作者：陈源闽崔世茂赵彦新居直祐内蒙古农牧业科学院蔬菜研究所呼和浩特010031 内蒙古农业大学农学院呼和浩特010019 日本名城大学农学部

选择不同地域的11个胡萝卜品种为试材,研究不同程度NaCI胁迫对其种子萌发的影响。结果表明,低浓度NaCI对个别胡萝卜品种种子萌发有促进作用,而对大多数胡萝卜品种的发芽具有抑制作用。不同品种胡萝卜种子萌发受NaCI胁迫抑制的程度不同,... 详细信息

选择不同地域的11个胡萝卜品种为试材,研究不同程度NaCI胁迫对其种子萌发的影响。结果表明,低浓度NaCI对个别胡萝卜品种种子萌发有促进作用,而对大多数胡萝卜品种的发芽具有抑制作用。不同品种胡萝卜种子萌发受NaCI胁迫抑制的程度不同,随着NaCl胁迫浓度的增大,对种子萌发的抑制作用明显增强。在发芽期的耐盐性方面,11个品种间存在明显差异,齐头黄,鞭杆红及06211耐盐性较强,阳明五寸,阳州五寸,yinari五寸,时无五寸,新大型五寸耐盐性较弱,其他品种耐盐性中等。胡萝卜品种的耐盐性强弱与其地域有关。

关键词： NaCI胁迫胡萝卜种子萌发

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水分胁迫对胡萝卜储藏器官糖代谢的影响

华北农学报 2007年第5期22卷 90-92页

作者：陈源闽崔世茂郝春燕新居■ 李晓静内蒙古农牧业科学院蔬菜研究所内蒙古呼和浩特010031 内蒙古农业大学农学院内蒙古呼和浩特010019 上海市宝山区蔬菜科学技术推广站上海200443 日本名城大学农学部

为探讨水分胁迫对胡萝卜储藏器官糖代谢的影响,为缺水地区科学栽培胡萝卜提供理论依据,以栽培胡萝卜品种"向阳二号"的6叶期幼苗为试材,研究了不同程度水分胁迫条件下胡萝卜储藏器官对葡萄糖、果糖、蔗糖及淀粉代谢的变化。结果表明,在... 详细信息

为探讨水分胁迫对胡萝卜储藏器官糖代谢的影响,为缺水地区科学栽培胡萝卜提供理论依据,以栽培胡萝卜品种"向阳二号"的6叶期幼苗为试材,研究了不同程度水分胁迫条件下胡萝卜储藏器官对葡萄糖、果糖、蔗糖及淀粉代谢的变化。结果表明,在水分胁迫下,蔗糖水平明显降低,胁迫程度越大,蔗糖水平越低,而葡萄糖和果糖水平却随之提高。淀粉含量的变化也是随着水分胁迫程度的增强而减少的,其中木质部中淀粉含量的变化要比韧皮部中的大。

关键词：胡萝卜水分胁迫储藏器官糖代谢