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4株AmpC启动子和衰减子突变大肠埃希菌的分析研究

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上海医学检验杂志 2002年第4期17卷 204-207页

作者：蒋燕群倪语星 nancy d.hanson 上海市第六人民医院检验科上海200233 上海第二医科大学附属瑞金医院临床微生物科上海200025 美国Creighton大学抗感染和分子生物学研究中心 omaha68178

目的　研究启动子和衰减子突变与大肠埃希菌高产AmpC酶的关系。方法　用琼脂稀释法、双纸片法、三相试验、等电聚焦电泳 (IEF)和聚合酶链反应 (PCR)检测其耐药机制 ,并对其AmpC的启动子和衰减子直接进行基因序列分析。衰减子的发夹状... 详细信息

目的　研究启动子和衰减子突变与大肠埃希菌高产AmpC酶的关系。方法　用琼脂稀释法、双纸片法、三相试验、等电聚焦电泳 (IEF)和聚合酶链反应 (PCR)检测其耐药机制 ,并对其AmpC的启动子和衰减子直接进行基因序列分析。衰减子的发夹状二级结构用dNASIS软件分析。结果　琼脂稀释法、双纸片法、三相试验、IEF和PCR证明这 4株菌都产染色体AmpC酶 ,基因序列分析提示它们在启动子和衰减子上都有不同程度的突变 ,同时还观察到其中一株菌的衰减子的G C发夹状二级结构由于突变导致△G 7.6kcal/mol的变化。

关键词： AmpC 启动子衰减子大肠埃希菌

Use of Patient-Specific “4d” Tele-Education to Enhance Actual and Perceived Knowledge in Congenital Heart disease (CHd) Patients

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Congenital Heart disease 2024年第1期19卷 5-17页

作者： Molly Clarke Karin Hamann nancy Klein Laura Olivieri Yue-Hin Loke department of Inpatient Cardiology Boston Children’s HospitalBoston02115USA department of Cardiology Children’s National HospitalWashington20001USA department of Cardiology Noninvasive Cardiac ImagingUniversity of Pittsburgh Medical Center Children’s Hospital of PittsburghPittsburgh15224USA

Background:Patients with congenital heart disease(CHd)will transition to lifelong adult congenital cardiac ***,their structural heart disease is challenging to convey via two-dimensional *** study utilized a tele-educational environment,with personalized three-dimensional(3d)modeling and health details(3d+details=“4d”),to improve actual and perceived knowledge,both important components of transition readiness in CHd ***:Participants aged≥13 years with a history of CHd and cardiac magnetic resonance imaging(MRI)studies were *** MRI datasets were then used to segment and create 3d heart models(using Mimics,Materialize Inc.).Participantsﬁrst completed the MyHeart Questionnaire,a validated survey of actual knowledge.A tele-educational session was then scheduled,during which participants were shown a 3d model of a normal heart,followed by their personal 3d heart model and speciﬁc health *** then repeated the actual knowledge survey,in addition to questionnaires assessing perceived knowledge pre-and post-session,as well as a satisfaction ***:Twenty-two patients were *** knowledge increased from 75%±15%to 89%±20%(p=0.00043)and perceived knowledge increased inﬁve of seven *** knowledge correlated with perceived knowledge(r=0.608,pd the 3d model as“very satisfactory”and ninety-ﬁve percent ranked the educational session as“very help-ful”or“extremely helpful.”Conclusions:The use of“4d”tele-education increased both actual and perceived knowledge and may help improve transition readiness in CHd patients.

关键词： Congenital heart disease tele-education transition

A Myosin XI Tail domain Homologous to the Yeast Myosin Vacuole-Binding domain Interacts with Plastids and Stromules in Nicotiana benthamiana

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Molecular Plant 2009年第6期2卷 1351-1358页

作者： Amir Sattarzadeh Johanna Krahmer Arnaud d. Germain Maureen R. hanson department of Molecular Biology and Genetics Biotechnology Building Cornell University Ithaca NY 14853 USA

The actin cytoskeleton plays a role in mobility of many different organelles in plant cells, including chloroplasts, mitochondria, Golgi, and peroxisomes. While progress has been made in identifying the myosin motors involved in trafficking of various plant organelles, not all of the cargoes mobilized by different members of the myosin XI family have yet been identified. The involvement of myosins in chloroplast positioning and mitochondrial movement was demonstrated by expression of a virus-induced gene silencing （VIGS） construct in tobacco. When VIGS with two different conserved sequences from a myosin Xl motor was performed in plants with either GFP-labeled plastids or mitochondria, chloroplast positioning in the dark was abnormal, and mitochondrial movement ceased. Because these and prior obser- vations have implicated a role for myosins and the actin cytoskeleton in plastid and stromule movement, we searched for myosin tail domains that could associate with plastids and stromules. While a yellow fluorescent protein （YFP） fusion with the entire tail region of myosin XI-F was usually found only in the cytoplasm, we observed that an Arabidopsis or Nicotiana benthamiana YFP：：myosin XI-F tail domain homologous to the yeast myo2p vacuole-binding domain associated with plastids and stromules after transient expression in N. benthamiana. Taken together, these observations implicate myosin motor proteins in dynamics of plastids and stromules.

关键词： Chloroplast biology cytoskeleton dynamics mitochondria gene silencing fluorescent protein.

碧口群火山岩的时代——SHRIMP锆石U-Pb测年结果

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地质通报 2003年第6期22卷 456-458页

作者：闫全人王宗起闫臻 Andrew d.hanson Peter A.druschke 刘敦一宋彪简平王涛中国地质科学院地质研究所北京100037 中国科学院地质与地球物理研究所北京100029 department of Geosciences University of Nevada Las Vegas

碧口群火山岩系是从原碧口系(叶连俊等,1944,后称碧口群)中解体出来的一个岩石单元(陶洪祥等,1993).

关键词：碧口群火山岩 SHRIMP锆石U—Pb测年地质时代扬子板块新元古代

Timing and setting of Guan-jiagou conglomerate in South Qinling and their tectonic im-plications

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Chinese Science Bulletin 2004年第16期49卷 1722-1729页

作者： YANQuanren A.d.hanson WANGZongqi P.A.druschkel YANZhen WANGTao LUHaifeng InstituteofGeology.ChineseAcademyofGeologicalSciences Beijing100037China departmentofGeosciences UniversityofNevadaLasVegasLasVegas454010NV89154USA InstituteofGeologyandGeophysics ChineseAcademyofSciencesBeijing100029China

Located in the southern Qinling Mountains of central China, the Guanjiagou Formation has been a con-troversial issue with regard to its depositional age and tec-tonic implications. Being comprised of an approximately 2050 m thick succession of texturally and compositionally immature, presumed marine turbiditic sandstones and con-glomerate, the Guanjiagou Formation consists of an overall prograding- and coarsening-upward megasequence. Al-though bounded by faults on both its northern and southern margins, it is weakly metamorphosed and deformed. To the north is the devonian Sanhekou Group and to the south is the Neoproterozoic Hengdan Group. The lower portion of the sequence contains granitic and volcanic clasts (Guanjiagou conglomerate). The feldspars from these clasts were dated using the 40Ar/39Ar method. Two cooling ages of 219.690.49 and 216.460.59 Ma, for K-feldspar from a granitic clast and plagioclase from a volcanic clast, respectively, were obtained. These ages are identical to the time of regional igneous ac-tivities (ca. 240—220 Ma) and are interpreted as the prod-ucts of magmatism associated with collision in the Qinling orogenic belt in the Early Mesozoic, suggesting that the Guanjiagou Formation was deposited in the Norian of the Late Triassic, ca. 220 Ma. Therefore, 40Ar/39Ar and sedimen-tary analyses suggest that the Guanjiagou Formation con-tains sediments that may have filled in a remnant ocean ba-sin, which might be part of the Anyemaqen-Mianle ocean, or Tethys on the southern side of Central Orogenic Belt in China during the Late Paleozoic to Early Mesozoic.

关键词：关家沟成因 ^40Ar/^39Ar定年残余海洋盆地沉积年代构造意义

扬子板块北缘碧口群火山岩的地球化学特征及其构造环境

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岩石矿物学杂志 2004年第1期23卷 1-11页

作者：闫全人 Andrew d.hanson 王宗起闫臻 Peter A.druschke 王涛刘敦一宋彪姜春发中国地质科学院地质研究所北京100037 department of Geosciences University of Nevada 中国科学院地质与地球物理研究所北京100029

碧口群火山岩系中部的基性熔岩以拉斑系列为主 ,基性火山岩的Sr同位素初始比值和Nd同位素初始比值较低 ,分别为 0 .70 12 4 8～ 0 .70 4 4 13和 0 .5 110 80～ 0 .5 12 341,大部分样品的εNd(t) >0 ,表明母岩浆主要来自地幔源区。岩石... 详细信息

碧口群火山岩系中部的基性熔岩以拉斑系列为主 ,基性火山岩的Sr同位素初始比值和Nd同位素初始比值较低 ,分别为 0 .70 12 4 8～ 0 .70 4 4 13和 0 .5 110 80～ 0 .5 12 341,大部分样品的εNd(t) >0 ,表明母岩浆主要来自地幔源区。岩石显示明显的富2 0 7Pb及2 0 8Pb特征。在以部分熔融作用为主的演化过程中岩浆发生了一定程度的分异 ,LREE、大离子亲石元素等表现了从亏损到富集的变化特点。基性熔岩的稀土与微量元素表现出与俯冲碰撞型的弧区玄武岩相同的特点。分析表明 :碧口群火山岩系形成于岛弧环境 ,它与横丹群浊积岩系在扬子板块西北缘构筑了一个弧盆系统 ,其时代为新元古代。

关键词：地球化学 Sr-Nd—Pb同位素碧口群火山岩弧一盆系统扬子板块新元古代

Bacteriological Profile of Infections Encountered in a Pneumology Service in a Moderate-Income Country [Pneumology department of CHU Cocody (Côte d’Ivoire)]

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Open Journal of Respiratory diseases 2024年第1期14卷 1-11页

作者： Brou Jean Marcel Ahui Alitonde Eudoxie djegbeton Alima Kone Marc-Olivier Koffi Kouame Clarisse Elogne Mobio nancy Hermine Benjamin Kouraogo Erick Akouatia Constante Virginie Brou-Gode Kigninlman Horo Boko Alexandre Kouassi Ngoran Koffi Pneumology Service Teaching Hospital of Cocody Abidjan C&#244te d’Ivoire Medical School of Abidjan University Félix Houphou&#235t Boigny Abidjan C&#244te d’Ivoire Pasteur Institute of Cô te d’Ivoire Abidjan C&#244te d’Ivoire

Introduction: Infections represent a real public health problem aggravating the morbidity and mortality of hospitalized patients. Methods: This was a retrospective study with descriptive purposes over a period of 05 years, in the Pneumology department of the University Hospital of Cocody. Results: The average age in our population was 42 years. We observed a male predo- minance of 64.5%, with a sex ratio of 1.8. Medical history was dominated by HIV infection (23.5%), followed by tuberculosis (15.6%). Concerning lifestyle, smoking was found in 38% of cases. Symptoms progressed chronically in 80% of cases. An infectious syndrome was found in 75% of cases. Microbial culture was positive in 42% of cases. Pseudomonas aeruginosa and Klebsiella pneumoniae were encountered in 26.4% of cases each, followed by Echerichia coli in 10.4% of cases. Klebsiella pneumoniae in the first three years topped the list, but gradually. Pseudomonas aeruginosa maintained its leadership over the last three years. Pseudomonas aeruginosa isolates expressed a resistance rate of 9.8% to ceftazidime and 8.1% to imipenem;to aztreonam (36%), ticarcillin (33.3%) and levofloxacin. These strains were susceptible to fosfomycin (100%), mero- penem (96.6%) and amikacin (96%). For isolated strains of Entero- bacteria- ceae, resistance was observed about ticarcillin (83.3%) and amoxicillin clavula- nic acid (71.2%). Streptococcaceae showed resistance to tetracycline (69.2%) and erythromycin (50%). Over the years there has been an increase in re- sistance to amoxicillin-clavulanic acid ceftriaxone. The death rate was 14%. Conclusion: The bacterial profile of infections is dominated by germs respon- sible for nosocomial infection with significant mortality.

关键词： Bacterial Pleural Pneumonia Nosocomial Infection Sub-Saharan Africa Antibiotic Therapy

SHRIMP Age and Geochemistry of the Bikou Volcanic Terrane:Implications for Neoproterozoic Tectonics on the Northern Margin of the Yangtze Craton

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Acta Geologica Sinica(English Edition) 2003年第4期77卷 479-490页

作者： YANQuanren WANGZongqi A.d.hanson P.A.dRUSCHKE YANZhen LIUdunyi JIANPing SONGBiao WANGTao JIANGChunfa InstituteofGeology ChineseAcademyofGeologicalSciencesBeijing100037 departmentofGeosciences UniversityofNevadaLasVegasLasVegasNV89154-4010USA InstituteofGeologyandGeophysics ChineseAcademyofSciencesBeijing100029

The Bikou volcanic terrane is predominated by subalkaline tholeiitic lavas. Rock samples display lower initial ratios of Sr and Nd, 0.701248-0.704413 and 0.511080-0.512341 respectively. 207Pb and 208Pb are significantly enriched in the lavas. Most samples have positive εNd, which indicates that the magma was derived from EM-type mantle source, while a few samples with negative εNd indicate that there was contamination in the magma evolution. Magma differentiation is demonstrated by variations of LREE and LILE from depletion to enrichment. Additionally, normalized REE patterns and trace elements showed that lavas from the Bikou volcanic terrane have similar characteristics to those of basalts in arc settings caused by subduction and collision. Analyses showed that the Bikou volcanic terrane is a volcanic arc. New evidence proved that the Hengdan Group, north of the Bikou arc, is a turbidite terrane filling a forearc basin. Consequently, the Bikou volcanic terrane and the Hengdan turbidite terrane construct an arc-basin system. New SHRIMP ages showed that this arc-basin system developed on the northern margin of the Yangtze craton in the Neoproterozoic (846-776 Ma), and this arc-basin system is in agreement with the tectonic processes of Rodinia in the Neoproterzoic.

关键词： geochemistry Sr-Nd-Pb isotopes SHRIMP ages Bikou Volcanic Terrane southwestern Qinling Mts. Yangtze Craton Rodinia

Associations of accelerometry-measured and self-reported physical activity and sedentary behavior with skeletal muscle energetics:The Study of Muscle,Mobility and Aging(SOMMA)

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Journal of Sport and Health Science 2024年第5期13卷 621-630页

作者： Yujia(Susanna)Qiao Terri L.Blackwell Peggy M.Cawthon Paul M.Coen Steven R.Cummings Giovanna distefano Samaneh Farsijani daniel E.Forman Bret H.Goodpaster Stephen B.Kritchevsky Theresa Mau Frederico G.S.Toledo Anne B.Newman nancy W.Glynn department of Epidemiology School of Public HealthUniversity of PittsburghPittsburghPA 15261USA San Francisco Coordinating Center California Pacific Medical Center Research InstituteSan FranciscoCA 94143USA department of Epidemiology and Biostatistics University of CaliforniaSan FranciscoCA 94143USA Translational Research Institute AdventHealthOrlandoFL 32804USA department of Medicine(Geriatrics and Cardiology) University of Pittsburghand GeriatricsResearchEducationand Clinical Center(GRECC)VA Pittsburgh Healthcare SystemPittsburghPA 15261USA department of Internal Medicine Section on Gerontology and Geriatric MedicineWake Forest School of MedicineWinston-SalemNC 27109USA division of Endocrinology and Metabolism Department of MedicineUniversity of PittsburghPittsburghPA 15261USA

Background:Skeletal muscle energetics decline with age,and physical activity(PA)has been shown to offset these declines in older ***,many studies reporting these effects were based on self-reported PA or structured exercise ***,we examined the associations of accelerometry-measured and self-reported PA and sedentary behavior(SB)with skeletal muscle energetics and explored the extent to which PA and sedentary behavior would attenuate the associations of age with muscle ***:As part of the Study of Muscle,Mobility and Aging,enrolled older adults(n=879),810(age=76.4±5.0 years old,mean±Sd;58%women)had maximal muscle oxidative capacity measured ex vivo via high-resolution re spirometry of permeabilized myofibers(maximal oxidative phosphorylation(maxOXPHOS))and in vivo by ^(31)phosphorus magnetic resonance spectroscopy(maximal adenosine triphosphate(ATP_(max))).Accelerometry-measured sedentary behavior,light activity,and moderate-to-vigorous PA(MVPA)were assessed using a wrist-worn ActiGraph GT9X over 7 ***-reported sedentary behavior,MVPA,and all PA were assessed with the Community Healthy Activities Model Program for Seniors(CHAMPS)*** regression models with progressive covariate adjustments evaluated the associations of sedentary behavior and PA with muscle energetics,as well as the attenuation of the age/muscle energetics association by MVP A and sedentary *** a sensitivity analysis,we also examined activPAL-measured daily step count and time spent in sedentary behavior and their associations with muscle ***:Every 30 min/day more of ActiGraph-measured MVPA was associated with 0.65 pmol/(s×mg)higher maxOXPHOS and 0.012 mM/s higher ATP_(max)after adjusting for age,site/technician,and sex(pd with maxOXPHOS or ATP_(max).Meanwhile,every 30 min/day spent in ActiGraph-measured sedentary behavior was associated with 0.39 pmol/s×mg lower maxOXPHOS and0.

关键词： Aging Exercise Mitochondria